Information on EC 3.4.17.12 - carboxypeptidase M

647252

Substrates: the enzyme could inactivate or modulate the activity of peptide hormones either before or after their interaction with plasma membrane receptors
Products: -

additional information

Substrates: the enzyme controls the activity of kinins in the kidney, is involved in inflammation, and participates in the metabolism of growth factors that contain C-terminal Arg or Lys residues, e.g. the epidermal growth factor, the nerve growth factor, the hypatocyte growth factor, the macrophage-stimulating protein, or erythropoietin
Products: -

additional information

670291

Substrates: the enzyme might be involved in regulating the biological effects of complement anaphylatoxins at sites of tissue injury
Products: -

additional information

Substrates: the enzyme regulates peptide hormones and growth factors at the cell surface, and is active in the degradation of extracellular proteins in the membrane
Products: -

additional information

Substrates: the enzyme regulates peptide hormones and growth factors at the cell surface, and is active in the degradation of extracellular proteins in the membrane
Products: -

additional information

668124

Substrates: removing of the C-terminal Arg residue of the intact kinins and related peptides
Products: -

additional information

Substrates: using small substrates of the form benzoyl-Xaa-Arg CPM has the highest catalytic efficiency for substrates with the nonpolar aliphatic residues Ala and Met and aromatic amino acids (Phe, Tyr, Trp) in the penultimate place and the lowest with branched side chains (Ile)
Products: -

additional information

show all columns Go to Natural Substrate Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O

Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe + arginine

Rattus norvegicus

683099

Substrates: bradykinin
Products: -

EGF + H2O

des-Arg53-EGF + arginine

683693

Substrates: EGF, epidermal growth factor
Products: -

epidermal growth factor + H2O

dea-Arg53-epidermal growth factor + arginine

Substrates: -
Products: -

additional information

8 entries

additional information

Substrates: the enzyme may be responsible for control of kinins and epidermal growth factor as well as of EGF-like peptides
Products: -

additional information

647252

Substrates: the enzyme could inactivate or modulate the activity of peptide hormones either before or after their interaction with plasma membrane receptors
Products: -

additional information

additional information

670291

Substrates: the enzyme might be involved in regulating the biological effects of complement anaphylatoxins at sites of tissue injury
Products: -

additional information

Substrates: the enzyme regulates peptide hormones and growth factors at the cell surface, and is active in the degradation of extracellular proteins in the membrane
Products: -

additional information

Substrates: the enzyme regulates peptide hormones and growth factors at the cell surface, and is active in the degradation of extracellular proteins in the membrane
Products: -

additional information

additional information

show all columns Go to Metals and Ions Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Co2+

KCl

addition of 0.5 M NaCl to the assay buffer does not significantly alter the activities of the wilde-type enzyme with 0.2 mM dansyl-Ala-Arg or the E260Q mutant enzyme but does substantially increase the activity of the E260A mutant, 219%. The enhancement of E260A is not specific for NaCl, as similar increases are detected with other salts such as NaNO3, 249%, KNO3, 256%, KCl, 256%, or Na2SO4, 297%

KNO3

Na2SO4

NaCl

NaNO3

Zn2+

additional information

the enzyme is a metallopeptidase

Co2+

CoCl2 activates

Co2+

CoCl2 activates more than 6-fold at pH 5.5, less than 2-fold at pH 7.5

81303

Co2+

activates, best at acidic pH

Zn2+

Zn2+

the enzyme is a metallopeptidase, binding to His66

670291

Zn2+

the catalytic center is located in the catalytic domain in a cavity with a funnel-like access that contains the catalytic Zn2+ ion

show all columns Go to Inhibitor Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

(2-guanidinoethylmercapto)succinic acid

1,10-phenanthroline

1,4-dithiothreitol

2-Guanidinoethylmercaptosuccinic acid

strong CPM inhibitor

2-mercaptomethyl-3-guanidinoethyl thiopropanoic acid

2-mercaptomethyl-3-guanidinoethylthiopropanoic acid

697627

bathophenanthroline disulfonic acid

Cadmium acetate

Cd(CH3COO)2

Cd2+

Co2+

DL-2-mercaptomethyl-3-guanidino-ethylthiopropanoic acid

DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid

DL-mercaptomethyl-3-guanidino-ethylthiopropanoic acid

EDTA

Guanidinoethyl mercaptosuccinic acid

Guanidinoethylmercaptosuccinic acid

MERGETPA

Zn2+

additional information

Gallus gallus

expression level of the gene carboxypeptidase M decreased after a Salmonella infection

684105

(2-guanidinoethylmercapto)succinic acid

(2-guanidinoethylmercapto)succinic acid

Cricetulus griseus

1,10-phenanthroline

weak

1,10-phenanthroline

1,10-phenanthroline

1 mM, 96% inhibition

1,10-phenanthroline

1,10-phenanthroline

2-mercaptomethyl-3-guanidinoethyl thiopropanoic acid

647252

2-mercaptomethyl-3-guanidinoethyl thiopropanoic acid

strong CPM inhibitor

DL-2-mercaptomethyl-3-guanidino-ethylthiopropanoic acid

Bos taurus

683200

DL-2-mercaptomethyl-3-guanidino-ethylthiopropanoic acid

683200, 684066

DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid

specific carboxypeptidase M inhibitor

DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid

Cricetulus griseus

specific carboxypeptidase M inhibitor

DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid

inhibits best at acidic pH

Guanidinoethyl mercaptosuccinic acid

1 mM, 97% inhibition

Guanidinoethyl mercaptosuccinic acid

81303

show all columns Go to Activating Compound Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

G-CSF

granulocyte-colony stimulating factor

684066

IFN-gamma

CPM activity can be upregulated approximately two-fold in HLMVECs by IL-1beta plus IFN-gamma treatment

683200

IL-1beta

CPM activity can be upregulated approximately two-fold in HLMVECs by IL-1beta plus IFN-gamma treatment, IL-1beta alone can upregulate CPM expression

683200

lipopolysaccharide

following stimulation with bacterial lipopolysaccharide a marginal upregulation of the expression of carboxypeptidase M is observed

683544

additional information

additional information

there is a spatial proximity and interaction between angiotensin-converting enzyme and endogenous carboxypeptidase M in MDCK cells, as well as a weak but detectable release of glycosylphosphatidylinositol-anchored carboxypeptidase M evoked by co-localized between angiotensin-converting enzyme

additional information

Cricetulus griseus

there is a spatial proximity and interaction between angiotensin-converting enzyme and endogenous carboxypeptidase M in CHO cells, as well as a weak but detectable release of glycosylphosphatidylinositol-anchored carboxypeptidase M evoked by co-localized between angiotensin-converting enzyme

additional information

prolactin/17beta-estradiol does not increase carboxypeptidase M expression

697627

Show Disease (1206 entries)

Go to Disease Search

show all columns Go to KM Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.033

Ala-Ser-His-Leu-Gly-Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

0.115 - 0.18

benzoyl-Ala-Arg

0.343

benzoyl-Asn-Arg

CPMwt, pH 7.4, 37°C

0.127

benzoyl-Gln-Arg

CPMwt, pH 7.4, 37°C

0.255 - 0.29

benzoyl-Gly-Arg

0.046 - 0.049

benzoyl-Gly-argininic acid

10.15 - 10.65

Benzoyl-Gly-Lys

0.239

benzoyl-His-Arg

CPMwt, pH 7.4, 37°C

0.205

benzoyl-Ile-Arg

CPMwt, pH 7.4, 37°C

0.168

benzoyl-Leu-Arg

CPMwt, pH 7.4, 37°C

0.13 - 0.138

benzoyl-Met-Arg

0.0584

benzoyl-Phe-Arg

CPMwt, pH 7.4, 37°C

0.219

benzoyl-Ser-Arg

CPMwt, pH 7.4, 37°C

0.192

benzoyl-Thr-Arg

CPMwt, pH 7.4, 37°C

0.069

benzoyl-Trp-Arg

CPMwt, pH 7.4, 37°C

0.048

benzoyl-Tyr-Arg

CPMwt, pH 7.4, 37°C

0.264

benzoyl-Val-Arg

CPMwt, pH 7.4, 37°C

0.016

bradykinin

0.0277

bradykinin(-Phe-Arg)

CPMwt, pH 7.2, 37°C

0.059 - 0.28

Dansyl-Ala-Arg

5 entries

0.068 - 0.09

furylacryloyl-Ala-Arg

1.152

furylacryloyl-Ala-Lys

CPMwt, pH 7.2, 37°C

1.181

Gly-Gly-Arg

CPMwt, pH 7.2, 37°C

0.05

Gly-Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

0.028

His-Leu-Gly-Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

0.027

Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

0.041

Leu-Gly-Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

0.063

Leu5-Arg6-enkephalin

0.046

Met5-Arg6-enkephalin

0.375

Met5-Lys6-enkephalin

0.206 - 0.241

N-(4-hydroxybenzoyl)-Gly-Arg

0.035

Pro-Gly-Lys-Ala-Arg

CPMwt, pH 7.2, 37°C

0.051

Ser-His-Leu-Gly-Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

0.115

benzoyl-Ala-Arg

CPMwt, pH 7.4, 37°C

0.162

benzoyl-Ala-Arg

CPMwt, pH 7.2, 37°C

0.18

benzoyl-Ala-Arg

hCPM, pH 7.2, 37°C

0.255

benzoyl-Gly-Arg

CPMwt, pH 7.2, 37°C

0.289

benzoyl-Gly-Arg

CPMwt, pH 7.4, 37°C

0.29

benzoyl-Gly-Arg

hCPM, pH 7.2, 37°C

0.046

benzoyl-Gly-argininic acid

CPMwt, pH 7.2, 37°C

0.049

benzoyl-Gly-argininic acid

hCPM, pH 7.2, 37°C

10.15

Benzoyl-Gly-Lys

hCPM, pH 7.2, 37°C

10.65

Benzoyl-Gly-Lys

CPMwt, pH 7.2, 37°C

0.13

benzoyl-Met-Arg

CPMwt, pH 7.2, 37°C

0.137

benzoyl-Met-Arg

hCPM, pH 7.2, 37°C

0.138

benzoyl-Met-Arg

CPMwt, pH 7.4, 37°C

0.016

bradykinin

0.016

bradykinin

purified CPM in vitro

683685

0.059

Dansyl-Ala-Arg

wild-type enzyme lacking the C-terminal hydrophobic membrane anchor signal

0.073

Dansyl-Ala-Arg

hCPM, pH 7.2, 37°C

0.083

Dansyl-Ala-Arg

CPMwt, pH 7.2, 37°C

0.102

Dansyl-Ala-Arg

mutant enzyme E260Q

0.28

Dansyl-Ala-Arg

mutant enzyme E260A

0.068

furylacryloyl-Ala-Arg

hCPM, pH 7.2, 37°C

0.09

furylacryloyl-Ala-Arg

CPMwt, pH 7.2, 37°C

0.206

N-(4-hydroxybenzoyl)-Gly-Arg

CPMwt, pH 7.2, 37°C

show all columns Go to Turnover Number Search

0.241

N-(4-hydroxybenzoyl)-Gly-Arg

hCPM, pH 7.2, 37°C

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

7.4

Ala-Ser-His-Leu-Gly-Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

77 - 107

benzoyl-Ala-Arg

benzoyl-Asn-Arg

CPMwt, pH 7.4, 37°C

benzoyl-Gln-Arg

CPMwt, pH 7.4, 37°C

3.9 - 6.07

benzoyl-Gly-Arg

273 - 316

benzoyl-Gly-argininic acid

9.6 - 11

Benzoyl-Gly-Lys

benzoyl-His-Arg

CPMwt, pH 7.4, 37°C

benzoyl-Ile-Arg

CPMwt, pH 7.4, 37°C

benzoyl-Leu-Arg

CPMwt, pH 7.4, 37°C

87 - 130

benzoyl-Met-Arg

benzoyl-Phe-Arg

CPMwt, pH 7.4, 37°C

benzoyl-Ser-Arg

CPMwt, pH 7.4, 37°C

22.9

benzoyl-Thr-Arg

CPMwt, pH 7.4, 37°C

benzoyl-Trp-Arg

CPMwt, pH 7.4, 37°C

benzoyl-Tyr-Arg

CPMwt, pH 7.4, 37°C

7.7

benzoyl-Val-Arg

CPMwt, pH 7.4, 37°C

2.45

bradykinin

bradykinin(-Phe-Arg)

CPMwt, pH 7.2, 37°C

0.233 - 17.6

Dansyl-Ala-Arg

5 entries

32 - 40

furylacryloyl-Ala-Arg

furylacryloyl-Ala-Lys

CPMwt, pH 7.2, 37°C

1.9

Gly-Gly-Arg

CPMwt, pH 7.2, 37°C

8.2

Gly-Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

6.6

His-Leu-Gly-Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

4.2

Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

6.1

Leu-Gly-Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

1.77

Leu5-Arg6-enkephalin

15.6

Met5-Arg6-enkephalin

11.1

Met5-Lys6-enkephalin

4.9 - 5.7

N-(4-hydroxybenzoyl)-Gly-Arg

Pro-Gly-Lys-Ala-Arg

CPMwt, pH 7.2, 37°C

6.7

Ser-His-Leu-Gly-Leu-Ala-Arg

CPMwt, pH 7.4, 37°C

benzoyl-Ala-Arg

CPMwt, pH 7.4, 37°C

benzoyl-Ala-Arg

hCPM, pH 7.2, 37°C

107

benzoyl-Ala-Arg

CPMwt, pH 7.2, 37°C

3.9

benzoyl-Gly-Arg

CPMwt, pH 7.4, 37°C

5.7

benzoyl-Gly-Arg

CPMwt, pH 7.2, 37°C

6.07

benzoyl-Gly-Arg

hCPM, pH 7.2, 37°C

273

benzoyl-Gly-argininic acid

hCPM, pH 7.2, 37°C

316

benzoyl-Gly-argininic acid

CPMwt, pH 7.2, 37°C

9.6

Benzoyl-Gly-Lys

hCPM, pH 7.2, 37°C

Benzoyl-Gly-Lys

CPMwt, pH 7.2, 37°C

benzoyl-Met-Arg

CPMwt, pH 7.4, 37°C

112

benzoyl-Met-Arg

hCPM, pH 7.2, 37°C

130

benzoyl-Met-Arg

CPMwt, pH 7.2, 37°C

2.45

bradykinin

2.45

bradykinin

purified CPM in vitro

683685

0.233

Dansyl-Ala-Arg

mutant enzyme E260A

5.12

Dansyl-Ala-Arg

wild-type enzyme lacking the C-terminal hydrophobic membrane anchor signal