Crystallization (Comment) | Organism |
---|---|
- |
Caldithrix abyssi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete loss of activity | Caldithrix abyssi | |
additional information | activities in phosphate buffers are singnificantly lower than in Tris-Hcl buffer | Caldithrix abyssi | |
o-phenanthroline | complete loss of activity | Caldithrix abyssi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
Arg-Arg-2-naphtylamide | pH 7.0, 25°C | Caldithrix abyssi | |
0.035 | - |
Arg-Arg-2-naphtylamide | pH 7.0, 50°C | Caldithrix abyssi | |
0.329 | - |
Gly-Arg-2-naphtylamide | pH 7.0, 50°C | Caldithrix abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | the zinc ion is essential for the enzyme activity. It is positioned in the lower part of the upper domain where it is pentacoordinated by two histidines | Caldithrix abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldithrix abyssi | H1XW48 | - |
- |
Caldithrix abyssi DSM 13497 | H1XW48 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-2-naphtylamide + H2O | 103% of the activity with Arg-Arg-2-naphtylamide | Caldithrix abyssi | Ala-Ala + 2-naphthylamine | - |
? | |
Ala-Ala-2-naphtylamide + H2O | 103% of the activity with Arg-Arg-2-naphtylamide | Caldithrix abyssi DSM 13497 | Ala-Ala + 2-naphthylamine | - |
? | |
Ala-Arg-2-naphtylamide + H2O | 65% of the activity with Arg-Arg-2-naphtylamide | Caldithrix abyssi | Ala-Arg + 2-naphthylamine | - |
? | |
Ala-Arg-2-naphtylamide + H2O | 65% of the activity with Arg-Arg-2-naphtylamide | Caldithrix abyssi DSM 13497 | Ala-Arg + 2-naphthylamine | - |
? | |
Arg-Arg-2-naphtylamide + H2O | - |
Caldithrix abyssi | Arg-Arg + 2-naphthylamine | - |
? | |
Arg-Arg-2-naphtylamide + H2O | - |
Caldithrix abyssi DSM 13497 | Arg-Arg + 2-naphthylamine | - |
? | |
Gly-Arg-2-naphtylamide + H2O | 167% of the activity with Arg-Arg-2-naphtylamide | Caldithrix abyssi | Gly-Arg + 2-naphthylamine | - |
? | |
Gly-Arg-2-naphtylamide + H2O | 167% of the activity with Arg-Arg-2-naphtylamide | Caldithrix abyssi DSM 13497 | Gly-Arg + 2-naphthylamine | - |
? | |
Phe-Arg-2-naphtylamide + H2O | 129% of the activity with Arg-Arg-2-naphtylamide | Caldithrix abyssi | Phe-Arg + 2-naphthylamine | - |
? | |
Phe-Arg-2-naphtylamide + H2O | 129% of the activity with Arg-Arg-2-naphtylamide | Caldithrix abyssi DSM 13497 | Phe-Arg + 2-naphthylamine | - |
? | |
Pro-Arg-2-naphtylamide + H2O | 143% of the activity with Arg-Arg-2-naphtylamide | Caldithrix abyssi | Pro-Arg + 2-naphthylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cabys_2252 | - |
Caldithrix abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Caldithrix abyssi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Arg-Arg-2-naphtylamide | pH 7.0, 25°C | Caldithrix abyssi | |
2.53 | - |
Gly-Arg-2-naphtylamide | pH 7.0, 50°C | Caldithrix abyssi | |
3.07 | - |
Arg-Arg-2-naphtylamide | pH 7.0, 50°C | Caldithrix abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Caldithrix abyssi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.63 | - |
Arg-Arg-2-naphtylamide | pH 7.0, 25°C | Caldithrix abyssi | |
7.67 | - |
Gly-Arg-2-naphtylamide | pH 7.0, 50°C | Caldithrix abyssi | |
87.3 | - |
Arg-Arg-2-naphtylamide | pH 7.0, 50°C | Caldithrix abyssi |