Cloned (Comment) | Organism |
---|---|
into the pET21b vector for expression in Escherichia coli BL21DE3RIL+ cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y318F | the potential functional role of the well-conserved tyrosine 318 residue in the active center of human DPP III is investigated | Homo sapiens |
Y395F | the potential functional role of the well-conserved tyrosine 395 residue in the active center of human DPP III is investigated | Homo sapiens |
Y644F | the potential functional role of the well-conserved tyrosine 644 residue in the active center of human DPP III is investigated | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tyr-Phe-NHOH | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
Arg-Arg-2-naphthylamide | mutant Y644F | Homo sapiens | |
0.0084 | - |
Arg-Arg-2-naphthylamide | wild-type enzyme | Homo sapiens | |
0.0104 | - |
Arg-Arg-2-naphthylamide | mutant Y318F | Homo sapiens | |
0.0114 | - |
Arg-Arg-2-naphthylamide | mutant Y395F | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
predicted, and verified by SDS-PAGE and Western Blot analysis, purified human erythtocyte DPP III and recombinant DPP III | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NY33 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration on a Sephacryl S-200 column, followed by FPLC on a Mono Q column, recombinant and human erythtocyte DPP III is purified | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Arg-2-naphthylamide + H2O | substrate used in the activity assay | Homo sapiens | Arg-Arg + 2-naphthylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dipeptidyl peptidase III | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
Arg-Arg-2-naphthylamide | mutant Y318F | Homo sapiens | |
8 | - |
Arg-Arg-2-naphthylamide | mutant Y395F | Homo sapiens | |
27.3 | - |
Arg-Arg-2-naphthylamide | mutant Y644F | Homo sapiens | |
31.5 | - |
Arg-Arg-2-naphthylamide | wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
activity assay | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000146 | - |
Tyr-Phe-NHOH | wild-type DPP III at pH 8.0 | Homo sapiens | |
0.000234 | - |
Tyr-Phe-NHOH | mutant Y318F at pH 8.0 | Homo sapiens |