Literature summary for 3.4.11.9 extracted from

Giannoglou, M.; Alexandrakis, Z.; Stavros, P.; Katsaros, G.; Katapodis, P.; Nounesis, G.; Taoukis, P.
Effect of high pressure on structural modifications and enzymatic activity of a purified X-prolyl dipeptidyl aminopeptidase from Streptococcus thermophilus (2018), Food Chem., 248, 304-311 .

Search for more literature for 3.4.11.9

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	activation of the enzyme is observed after processing at 100-200 MPa and 20-30°C	Streptococcus thermophilus

Application

Application	Comment	Organism
food industry	PepX aminopeptidase from Streptococcus thermophilus ACA DC 0022 is used in Greek Feta cheese manufacturing	Streptococcus thermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	more intense processing conditions, above 100-200 MPa and 20-30°C, lead to enzyme inactivation with PepX HP-induced conformational changes, investigated by circular dichroism spectroscopy, kinetic analysis, overview	Streptococcus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
175000	-	native PAGE	Streptococcus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Streptococcus thermophilus	-	-	-
Streptococcus thermophilus ACA DC 0022	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme by anion exchange chromatography, ultrafiltration, gel filtration, and hydroxyapatite chromatography, to homogeneity	Streptococcus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Gly-Pro-4-nitroanilide + H2O	-	Streptococcus thermophilus	Gly + Pro-4-nitroanilide	-	?
Gly-Pro-4-nitroanilide + H2O	-	Streptococcus thermophilus ACA-DC 0022	Gly + Pro-4-nitroanilide	-	?
Gly-Pro-4-nitroanilide + H2O	-	Streptococcus thermophilus ACA DC 0022	Gly + Pro-4-nitroanilide	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 86000, SDS-PAGE	Streptococcus thermophilus
More	pressures up to 200 MPa result in a structurally molten globule-like state where PepX maintains its secondary structure but the tertiary structure is substantially affected and enzyme activity increases. Both secondary and tertiary structures are affected severely by higher pressures (450 MPa), which reduce enzyme activity	Streptococcus thermophilus

Synonyms

Synonyms	Comment	Organism
PepX aminopeptidase	-	Streptococcus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	activation of the enzyme is observed after processing at 100-200 MPa and 20-30°C, kinetic analysis, overview	Streptococcus thermophilus
30	-	assay at	Streptococcus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Streptococcus thermophilus

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Release 2023.1 (January 2023)