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Literature summary for 3.4.11.9 extracted from

  • Simmons, W.H.; Orawski, A.T.
    Membrane-bound aminopeptidase P from bovine lung. Its purification, properties, and degradation of bradykinin (1992), J. Biol. Chem., 267, 4897-4903.
    View publication on PubMed
Search for more literature for 3.4.11.9

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Bos taurus
NaCl above 0.25 M Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.076
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 Bos taurus
0.15
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe
-
 Bos taurus
0.15
-
 Arg-Pro-Pro-Gly-Phe-Ser
-
 Bos taurus
0.25
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro
-
 Bos taurus
0.34
-
 Arg-Pro-Pro-Gly-Phe
-
 Bos taurus
0.76
-
 Arg-Pro-Pro
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane attached to the membranes via a glycosylphosphatidylinositol anchor Bos taurus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 x * 95000, reducing conditions, SDS-PAGE Bos taurus
360000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bos taurus the enzyme is involved in the pulmonary inactivation of circulating bradykinin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification the enzyme is attached to the membranes via a glycosylphosphatidylinositol anchor Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
-20°C, pH 6.8, stable for at least 1 month Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Pro-Pro + H2O
-
 Bos taurus Arg + Pro-Pro
-
 ?
Arg-Pro-Pro-Gly-Phe + H2O
-
 Bos taurus ?
-
 ?
Arg-Pro-Pro-Gly-Phe-Ser + H2O
-
 Bos taurus ?
-
 ?
Arg-Pro-Pro-Gly-Phe-Ser-Pro + H2O
-
 Bos taurus ?
-
 ?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe + H2O
-
 Bos taurus ?
-
 ?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O i.e. bradykinin Bos taurus Arg + Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 ?
additional information the enzyme favors peptides with 2 proline residues or proline analogs in position 2 and 3 of the substrate Bos taurus ?
-
 ?
additional information the enzyme is involved in the pulmonary inactivation of circulating bradykinin Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
tetramer x * 95000, reducing conditions, SDS-PAGE Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 6.8, 30 min, stable up to Bos taurus
75
-
 pH 6.8, 30 min, complete loss of activity Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 Bos taurus
30
-
 Arg-Pro-Pro-Gly-Phe-Ser
-
 Bos taurus
39
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe
-
 Bos taurus
41
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro
-
 Bos taurus
48
-
 Arg-Pro-Pro-Gly-Phe
-
 Bos taurus
120
-
 Arg-Pro-Pro
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 hydrolysis of Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg Bos taurus
7.5
-
-
 Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 11 37°C, 2 h, maximal stability Bos taurus