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Literature summary for 3.4.11.7 extracted from

  • Asano, M.; Nakamura, N.; Kawai, M.; Miwa, T.; Nio, N.
    Purification and characterization of an N-terminal acidic amino acid-specific aminopeptidase from soybean cotyledons (Glycine max) (2010), Biosci. Biotechnol. Biochem., 74, 113-118.
    View publication on PubMed
Search for more literature for 3.4.11.7

Inhibitors

Inhibitors Comment Organism Structure
DTT 32% activity at 0.1 mM Glycine max
EDTA 83.5% activity at 5 mM Glycine max
o-phenanthroline 47.1% activity at 2 mM Glycine max
Zn2+ 68.3% activity at 0.4 mM Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Glycine max
510000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
 Merril cv. Kegon
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, anion exchange chromatography, hydrophobic interaction chromatography, gel filtration Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
66.3
-
 substrate Glu-Glu, pH 7.2, 37°C Glycine max
132
-
 substrate Asp-4-nitroanilide, pH 7.2, 37°C Glycine max
319
-
 substrate Glu-4-nitroanilide, pH 7.2, 37°C Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Asp-4-nitroanilide + H2O
-
 Glycine max Asp + 4-nitroaniline
-
 ?
Glu-4-nitroanilide + H2O
-
 Glycine max Glu + 4-nitroaniline
-
 ?
Glu-Glu + H2O many Glu-Xxx and Asp-Xxx dipeptides are substrates Glycine max 2 Glu
-
 ?

Subunits

Subunits Comment Organism
multimer x * 56000, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
N-terminal acidic amino acid-specific aminopeptidase
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 pH 6.0 Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable for 80 min at pH 6.0 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
7 9 more than 80% activity at 30°C Glycine max