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alpha-L-Asp-2-naphthylamide + H2O
L-Asp + 2-naphthylamine
-
-
-
-
?
alpha-L-Asp-Val-angiotensin II + H2O
Asp + Val-angiotensin II
-
-
-
-
?
alpha-L-Glu-2-naphthylamide + H2O
alpha-L-Glu + 2-naphthylamine
-
-
-
-
?
alpha-L-Glu-beta-naphthylamide + H2O
?
-
-
-
-
?
alpha-L-Glu-beta-naphthylamide + H2O
L-Glu + 2-naphthylamine
-
-
-
-
?
angiotensin I + H2O
Asp + Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu
-
-
-
-
?
angiotensin II
Asp + Arg-Val-Tyr-Ile-His-Pro-Phe
angiotensin II + H2O
angiotensin III + Arg
angiotensin II + H2O
Asp + angiotensin III
angiotensin II + H2O
aspartic acid + angiotensin III
angiotensin II + H2O
L-Asp + angiotensin III
-
-
-
-
?
angiotensin II + H2O
L-aspartic acid + angiotensin III
angiotensin III + H2O
? + angiotensin IV
-
i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe, low activity in absence of Ca2+
-
-
?
angiotensin III + H2O
angiotensin IV + Arg
Asp-4-methylcoumaryl-7-amide + H2O
Asp + 7-amino-4-methylcoumarin
-
low activity in absence and presence of Ca2+
-
-
?
Asp-4-nitroanilide + H2O
Asp + 4-nitroaniline
-
-
-
-
?
Asp-7-amido-4-methylcoumarin + H2O
Asp + 7-amino-4-methylcoumarin
Q8I2J3;
assay for measuring aspartyl aminopeptidase activity
-
-
?
Asp-beta-naphthylamide + H2O
Asp + naphthylamine
Asp-Phe-NH2 + H2O
Asp + Phe + NH3
-
-
-
-
?
aspartame + H2O
L-Asp + L-Phe methyl ester
-
-
-
-
?
cholecystokinin-8 + H2O
?
cholecystokinin-8 + H2O
Asp + YMGWMDF-NH2
-
i.e. CCK8 or DYMGWMDF-NH2
-
-
?
cholecystokinin-8 + H2O
L-Asp + YMGWMDF-NH2
-
-
-
-
?
chromogranin A + H2O
?
-
-
-
-
?
chromogranin A + H2O
Glu + EEEEMAVVPQGLFRG-NH2
-
i.e. EEEEEMAVVPQGLFRG-NH2
-
-
?
Gln-4-methylcoumaryl-7-amide + H2O
Gln + 7-amino-4-methylcoumarin
-
low activity in absence and presence of Ca2+
-
-
?
Glu-2-naphthylamide + H2O
Glu + 2-naphthylamine
Glu-4-methylcoumaryl-7-amide + H2O
Glu + 7-amino-4-methylcoumarin
Glu-4-nitroanilide + H2O
Glu + 4-nitroaniline
Glu-7-amido-4-methylcoumarin + H2O
Gln + 7-amino-4-methylcoumarin
Glu-7-amido-4-methylcoumarin + H2O
Glu + 7-amino-4-methylcoumarin
Q8I2J3;
assay for measuring aspartyl aminopeptidase activity
-
-
?
Glu-beta-naphthylamide + H2O
Glu + naphthylamine
Glu-Glu + H2O
2 Glu
-
many Glu-Xxx and Asp-Xxx dipeptides are substrates
-
-
?
Glu-p-nitroanilide + H2O
Glu + 4-nitroaniline
Glu-p-nitroanilide + H2O
Glu + p-nitroaniline
Glu-p-nitroanilide + H2O
glutamic acid + p-nitroaniline
kallidin + H2O
?
-
-
-
-
?
kallidin + H2O
Lys + bradykinin
-
i.e. Lys-bradykinin or KRPPGFSPFR, the reaction is performed only in absence of Ca2+
i.e. RPPGFSPFR
-
?
L-Ala-4-methylcoumaryl-7-amide + H2O
L-Ala + 7-amino-4-methylcoumarin
L-Arg-2-naphthylamide + H2O
L-Arg + 2-naphthylamine
L-Asp-peptide + H2O
L-Asp + peptide
L-Glu-2-naphthylamide + H2O
L-Glu + 2-naphthylamine
L-Glu-4-methylcoumaryl-7-amide + H2O
L-Glu + 7-amino-4-methylcoumarin
L-Glu-7-amido-4-methylcoumarin + H2O
L-Glu + 7-amino-4-methylcoumarin
L-Glu-p-nitroanilide + H2O
L-Glu + p-nitroaniline
-
-
-
-
?
L-Glu-peptide + H2O
L-Glu + peptide
L-Leu-4-methylcoumaryl-7-amide + H2O
L-Leu + 7-amino-4-methylcoumarin
Leu-7-amido-4-methylcoumarin + H2O
Leu + 7-amino-4-methylcoumarin
Q8I2J3;
assay for measuring aspartyl aminopeptidase activity
-
-
?
milk proteins + H2O
?
-
-
-
-
-
N-(alpha-L-Ala-L-Leu-L-Lys-Arg)-2-naphthylamide + H2O
?
N-(alpha-L-aspartyl)-2-naphthylamide + H2O
L-aspartic acid + 2-naphthylamine
N-(alpha-L-aspartylyl)-2-naphthylamide + H2O
L-aspartic acid + 2-naphthylamine
N-(alpha-L-glutamyl)-2-naphthylamide + H2O
L-glutamic acid + 2-naphthylamine
N-(alpha-L-glutamyl)-4-nitroanilide + H2O
L-glutamic acid + 4-nitroaniline
N-(alpha-L-glutamyl)-beta-naphthylamide + H2O
L-glutamic acid + beta-naphthylamine
N-Glu-L-Phe-4-nitroanilide + H2O
L-Glu + L-Phe-4-nitroanilide
-
-
-
-
?
neurokinin B + H2O
?
-
-
-
-
?
neurokinin B + H2O
Asp + MHDFFVGLM-NH2
-
i.e. DMHDFFVGLM-NH2
-
-
?
additional information
?
-
Angiotensin I + H2O

?
-
cleavage only in vitro
-
-
?
Angiotensin I + H2O
?
-
cleavage only in vitro
-
-
?
Angiotensin I + H2O
?
-
cleavage only in vitro
-
-
?
Angiotensin I + H2O
?
-
cleavage only in vitro
-
-
?
angiotensin II

Asp + Arg-Val-Tyr-Ile-His-Pro-Phe
-
-
-
?
angiotensin II
Asp + Arg-Val-Tyr-Ile-His-Pro-Phe
-
-
-
?
Angiotensin II + H2O

?
-
-
-
-
-
Angiotensin II + H2O
?
-
-
-
-
?
angiotensin II + H2O

angiotensin III + Arg
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
i.e. Arg-Val-Tyr-Ile-His-Pro-Phe
-
ir
angiotensin II + H2O
angiotensin III + Arg
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
i.e. Arg-Val-Tyr-Ile-His-Pro-Phe
-
ir
angiotensin II + H2O
angiotensin III + Arg
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
i.e. Arg-Val-Tyr-Ile-His-Pro-Phe
-
ir
angiotensin II + H2O
angiotensin III + Arg
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
i.e. Arg-Val-Tyr-Ile-His-Pro-Phe
-
ir
angiotensin II + H2O

Asp + angiotensin III
-
angiotensin is the most active compound of the renin-angiotensin-system, RAS, involved in regulation of blood pressure in the brain
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
angiotensin II + H2O
Asp + angiotensin III
-
angiotensin is the most active compound of the renin-angiotensin-system, RAS, involved in renal development
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
angiotensin II + H2O
Asp + angiotensin III
-
the enzyme is responsible for the convertion of angiotensin II into angiotensin III in the brain
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
angiotensin is the most active compound of the renin-angiotensin-system, RAS, involved in renal development
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
angiotensin II + H2O
Asp + angiotensin III
-
angiotensin II is the principal effector of the renin-angiotensin-system, RAS, which induces vasoconstriction and increases sodium and water retention thereby leading to increased blood pressure
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
aspartyl aminopeptidase and several other aminopeptidases, i.e. angiotensinases, are involved in angiotensin II and cholecystokinin, CCK, metabolism, angiotensin II is the principal effector of the renin-angiotensin-system, RAS, which induces vasoconstriction and increases sodium and water retention thereby leading to increased blood pressure
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
angiotensin II + H2O

aspartic acid + angiotensin III
-
-
-
-
?
angiotensin II + H2O
aspartic acid + angiotensin III
-
angiotensin II: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
angiotensin III: Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
angiotensin II + H2O
aspartic acid + angiotensin III
-
-
-
-
?
angiotensin II + H2O
aspartic acid + angiotensin III
-
angiotensin II: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
angiotensin III: Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
angiotensin II + H2O
aspartic acid + angiotensin III
-
angiotensin II: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
angiotensin III: Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
angiotensin II + H2O
aspartic acid + angiotensin III
-
angiotensin II: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
angiotensin III: Arg-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
angiotensin II + H2O

L-aspartic acid + angiotensin III
-
-
-
-
?
angiotensin II + H2O
L-aspartic acid + angiotensin III
-
-
-
-
?
angiotensin III + H2O

angiotensin IV + Arg
-
the enzyme is involved in regulation of blood pressure in the renin-angiotensin system in the brain causing hypertension
-
-
ir
angiotensin III + H2O
angiotensin IV + Arg
-
the enzyme is involved in regulation of blood pressure in the renin-angiotensin system in the brain causing hypertension
-
-
ir
angiotensin III + H2O
angiotensin IV + Arg
-
the enzyme is involved in regulation of blood pressure in the renin-angiotensin system in the brain causing hypertension
-
-
ir
angiotensin III + H2O
angiotensin IV + Arg
-
the enzyme is involved in regulation of blood pressure in the renin-angiotensin system in the brain causing hypertension
-
-
ir
Asp-Ala + H2O

Asp + Ala
-
-
-
-
-
Asp-Ala + H2O
Asp + Ala
-
74% of the activity with Glu-Ala
-
-
?
Asp-beta-naphthylamide + H2O

Asp + naphthylamine
-
dog kidney enzyme 3 times more active compared to Glu-beta-naphthylamide
-
-
?
Asp-beta-naphthylamide + H2O
Asp + naphthylamine
-
-
-
-
?
Asp-beta-naphthylamide + H2O
Asp + naphthylamine
-
-
-
-
?
cholecystokinin-8 + H2O

?
-
i.e. CCK8
-
-
?
cholecystokinin-8 + H2O
?
-
cleavage only in vitro
-
-
?
cholecystokinin-8 + H2O
?
-
CCK8
-
-
?
cholecystokinin-8 + H2O
?
-
cleavage only in vitro
-
-
?
cholecystokinin-8 + H2O
?
-
cleavage only in vitro
-
-
?
cholecystokinin-8 + H2O
?
-
cleavage only in vitro
-
-
?
Glu-2-naphthylamide + H2O

Glu + 2-naphthylamine
-
synthetic substrate used in the activity assay
-
-
?
Glu-2-naphthylamide + H2O
Glu + 2-naphthylamine
-
synthetic substrate used in the activity assay
-
-
?
Glu-4-methylcoumaryl-7-amide + H2O

Glu + 7-amino-4-methylcoumarin
-
-
-
-
?
Glu-4-methylcoumaryl-7-amide + H2O
Glu + 7-amino-4-methylcoumarin
-
low activity in absence of Ca2+
-
-
?
Glu-4-nitroanilide + H2O

Glu + 4-nitroaniline
-
-
-
-
?
Glu-4-nitroanilide + H2O
Glu + 4-nitroaniline
Q04IU1;
-
-
-
?
Glu-7-amido-4-methylcoumarin + H2O

Gln + 7-amino-4-methylcoumarin
-
-
-
-
?
Glu-7-amido-4-methylcoumarin + H2O
Gln + 7-amino-4-methylcoumarin
-
activity assay
-
-
?
Glu-7-amido-4-methylcoumarin + H2O
Gln + 7-amino-4-methylcoumarin
-
-
-
-
?
Glu-7-amido-4-methylcoumarin + H2O
Gln + 7-amino-4-methylcoumarin
-
-
-
-
?
Glu-Ala + H2O

Glu + Ala
-
-
-
-
-
Glu-Ala + H2O
Glu + Ala
-
high activity
-
-
?
Glu-beta-naphthylamide + H2O

Glu + naphthylamine
-
aminopeptidase A six times more active compared to Asp-beta-naphthylamide
-
-
?
Glu-beta-naphthylamide + H2O
Glu + naphthylamine
-
-
-
-
?
Glu-beta-naphthylamide + H2O
Glu + naphthylamine
-
-
-
-
?
Glu-beta-naphthylamide + H2O
Glu + naphthylamine
-
-
-
-
?
Glu-beta-naphthylamide + H2O
Glu + naphthylamine
-
-
-
-
?
Glu-p-nitroanilide + H2O

Glu + 4-nitroaniline
-
peptidase activity assay
-
-
?
Glu-p-nitroanilide + H2O
Glu + 4-nitroaniline
-
peptidase activity assay
-
-
?
Glu-p-nitroanilide + H2O

Glu + p-nitroaniline
-
-
-
-
?
Glu-p-nitroanilide + H2O
Glu + p-nitroaniline
-
-
-
-
?
Glu-p-nitroanilide + H2O
Glu + p-nitroaniline
-
-
-
-
?
Glu-p-nitroanilide + H2O
Glu + p-nitroaniline
-
activity assay, cleaved p-nitroanilide is measured
-
-
?
Glu-p-nitroanilide + H2O
Glu + p-nitroaniline
-
-
-
-
?
Glu-p-nitroanilide + H2O
Glu + p-nitroaniline
-
-
-
-
?
Glu-p-nitroanilide + H2O
Glu + p-nitroaniline
-
-
-
-
?
Glu-p-nitroanilide + H2O

glutamic acid + p-nitroaniline
-
peptidase activity assay
-
-
?
Glu-p-nitroanilide + H2O
glutamic acid + p-nitroaniline
-
peptidase activity assay
-
-
?
Glu-p-nitroanilide + H2O
glutamic acid + p-nitroaniline
-
peptidase activity assay
-
-
?
Glu-p-nitroanilide + H2O
glutamic acid + p-nitroaniline
-
peptidase activity assay
-
-
?
Glu-p-nitroanilide + H2O
glutamic acid + p-nitroaniline
-
peptidase activity assay
-
-
?
Glu-p-nitroanilide + H2O
glutamic acid + p-nitroaniline
-
peptidase activity assay
-
-
?
Glu-p-nitroanilide + H2O
glutamic acid + p-nitroaniline
-
peptidase activity assay
-
-
?
Glu-p-nitroanilide + H2O
glutamic acid + p-nitroaniline
-
peptidase activity assay
-
-
?
Glu-p-nitroanilide + H2O
glutamic acid + p-nitroaniline
-
peptidase activity assay
-
-
?
L-Ala-4-methylcoumaryl-7-amide + H2O

L-Ala + 7-amino-4-methylcoumarin
about 50% of the activity with L-Glu-4-methylcoumaryl-7-amide
-
-
?
L-Ala-4-methylcoumaryl-7-amide + H2O
L-Ala + 7-amino-4-methylcoumarin
about 50% of the activity with L-Glu-4-methylcoumaryl-7-amide
-
-
?
L-Arg-2-naphthylamide + H2O

L-Arg + 2-naphthylamine
-
-
-
-
?
L-Arg-2-naphthylamide + H2O
L-Arg + 2-naphthylamine
-
-
-
-
?
L-Arg-2-naphthylamide + H2O
L-Arg + 2-naphthylamine
-
-
-
-
?
L-Asp-peptide + H2O

L-Asp + peptide
-
-
-
-
?
L-Asp-peptide + H2O
L-Asp + peptide
-
-
-
-
?
L-Asp-peptide + H2O
L-Asp + peptide
-
free gamma-carboxyl group of N-terminal amino acid essential
-
-
?
L-Asp-peptide + H2O
L-Asp + peptide
-
specificity for di- and tri-peptides with Asp or Glu at N-terminal end
-
-
?
L-Glu-2-naphthylamide + H2O

L-Glu + 2-naphthylamine
-
-
-
-
?
L-Glu-2-naphthylamide + H2O
L-Glu + 2-naphthylamine
-
-
-
-
?
L-Glu-4-methylcoumaryl-7-amide + H2O

L-Glu + 7-amino-4-methylcoumarin
-
-
-
?
L-Glu-4-methylcoumaryl-7-amide + H2O
L-Glu + 7-amino-4-methylcoumarin
-
-
-
?
L-Glu-7-amido-4-methylcoumarin + H2O

L-Glu + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Glu-7-amido-4-methylcoumarin + H2O
L-Glu + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Glu-peptide + H2O

L-Glu + peptide
-
-
-
-
?
L-Glu-peptide + H2O
L-Glu + peptide
-
-
-
-
?
L-Glu-peptide + H2O
L-Glu + peptide
-
specificity for di-and tripeptides with Asp or Glu at N-terminal end
-
-
?
L-Leu-4-methylcoumaryl-7-amide + H2O

L-Leu + 7-amino-4-methylcoumarin
-
about 20% of the activity with L-Glu-4-methylcoumaryl-7-amide
-
?
L-Leu-4-methylcoumaryl-7-amide + H2O
L-Leu + 7-amino-4-methylcoumarin
-
about 20% of the activity with L-Glu-4-methylcoumaryl-7-amide
-
?
N-(alpha-L-Ala-L-Leu-L-Lys-Arg)-2-naphthylamide + H2O

?
-
-
-
-
?
N-(alpha-L-Ala-L-Leu-L-Lys-Arg)-2-naphthylamide + H2O
?
-
-
-
-
?
N-(alpha-L-Ala-L-Leu-L-Lys-Arg)-2-naphthylamide + H2O
?
-
-
-
-
?
N-(alpha-L-Ala-L-Leu-L-Lys-Arg)-2-naphthylamide + H2O
?
-
-
-
-
?
N-(alpha-L-aspartyl)-2-naphthylamide + H2O

L-aspartic acid + 2-naphthylamine
-
-
-
-
?
N-(alpha-L-aspartyl)-2-naphthylamide + H2O
L-aspartic acid + 2-naphthylamine
-
-
-
-
?
N-(alpha-L-aspartylyl)-2-naphthylamide + H2O

L-aspartic acid + 2-naphthylamine
-
-
-
-
?
N-(alpha-L-aspartylyl)-2-naphthylamide + H2O
L-aspartic acid + 2-naphthylamine
-
-
-
-
?
N-(alpha-L-aspartylyl)-2-naphthylamide + H2O
L-aspartic acid + 2-naphthylamine
-
-
-
-
?
N-(alpha-L-aspartylyl)-2-naphthylamide + H2O
L-aspartic acid + 2-naphthylamine
-
-
-
-
?
N-(alpha-L-glutamyl)-2-naphthylamide + H2O

L-glutamic acid + 2-naphthylamine
-
-
-
-
?
N-(alpha-L-glutamyl)-2-naphthylamide + H2O
L-glutamic acid + 2-naphthylamine
-
-
-
-
?
N-(alpha-L-glutamyl)-2-naphthylamide + H2O
L-glutamic acid + 2-naphthylamine
-
-
-
-
?
N-(alpha-L-glutamyl)-2-naphthylamide + H2O
L-glutamic acid + 2-naphthylamine
-
-
-
-
?
N-(alpha-L-glutamyl)-4-nitroanilide + H2O

L-glutamic acid + 4-nitroaniline
-
-
-
-
?
N-(alpha-L-glutamyl)-4-nitroanilide + H2O
L-glutamic acid + 4-nitroaniline
-
-
-
-
?
N-(alpha-L-glutamyl)-4-nitroanilide + H2O
L-glutamic acid + 4-nitroaniline
-
-
-
-
?
N-(alpha-L-glutamyl)-beta-naphthylamide + H2O

L-glutamic acid + beta-naphthylamine
-
-
-
-
?
N-(alpha-L-glutamyl)-beta-naphthylamide + H2O
L-glutamic acid + beta-naphthylamine
-
-
-
-
?
N-(alpha-L-glutamyl)-beta-naphthylamide + H2O
L-glutamic acid + beta-naphthylamine
-
-
-
-
?
Ser-Ala + H2O

Ser + Ala
-
-
-
-
?
Ser-Ala + H2O
Ser + Ala
-
less than 2% of the activity with Glu-Ala
-
-
?
additional information

?
-
-
the enzyme catalyzes the hydrolysis of N-(alpha-L-glutamyl)- moieties more efficiently than the hydrolysis of alpha-L-aspartyl derivatives
-
-
-
additional information
?
-
-
slow hydrolysis of Ala-beta-naphthylamide, Arg-beta-naphthylamide
-
-
-
additional information
?
-
-
the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides, the enzyme is involved in metabolism of angiotensin II and angiotensin III in the brain, overview
-
-
-
additional information
?
-
-
substrate specificity in presence or absence of Ca2+
-
-
-
additional information
?
-
-
substrate specificity, overview, the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
the enzyme removes N-terminal Glu, Asp, and to a lesser extent also Ser residues from peptide substrates, no activity with Glu-substrate with a blocked N-terminus
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
the enzyme removes N-terminal Glu, Asp, and to a lesser extent also Ser residues from peptide substrates
-
-
-
additional information
?
-
-
association between elevated BP-1 expression and cell growth. The enzyme is not involved in immune system development
-
-
-
additional information
?
-
-
the S1' subsite is hydrophobic whereas the S2' subsite recognizes preferentially negatively charged residues derived from aspartic acid
-
-
-
additional information
?
-
-
The C-terminal domain of aminopeptidase A is an intramolecular chaperone required for the correct folding, cell surface expression, and activity of the enzyme
-
-
-
additional information
?
-
-
the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides, the enzyme is involved in metabolism of angiotensin II and angiotensin III in the brain, overview
-
-
-
additional information
?
-
-
the enzyme is involved in angiotensin metabolism
-
-
-
additional information
?
-
-
the enzyme is involved in conversion of angiotensin II to angiotensin III and thus in controlling of vasopressin release and blood pressure, overview
-
-
-
additional information
?
-
-
substrate specificity, overview, the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides
-
-
-
additional information
?
-
-
the enzyme catalyzes the hydrolysis of N-(alpha-L-glutamyl)- moieties more efficiently than the hydrolysis of alpha-L-aspartyl derivatives
-
-
-
additional information
?
-
-
administration of purified enzyme into hypertensive rats leads to a decrease in blood pressure, while enzyme inhibition increases blood pressure
-
-
-
additional information
?
-
-
the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides, the enzyme is involved in metabolism of angiotensin II and angiotensin III in the brain, overview
-
-
-
additional information
?
-
-
the enzyme is involved in conversion of angiotensin II to angiotensin III and thus in controlling of vasopressin release and blood pressure, overview
-
-
-
additional information
?
-
-
substrate specificity, overview, the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides
-
-
-
additional information
?
-
-
the enzyme catalyzes the hydrolysis of N-(alpha-L-glutamyl)- moieties more efficiently than the hydrolysis of alpha-L-aspartyl derivatives
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
the enzyme removes N-terminal Glu, Asp, and to a lesser extent also Ser residues from peptide substrates
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
the enzyme removes N-terminal Glu, Asp, and to a lesser extent also Ser residues from peptide substrates
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
the enzyme removes N-terminal Glu, Asp, and to a lesser extent also Ser residues from peptide substrates
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
the enzyme removes N-terminal Glu, Asp, and to a lesser extent also Ser residues from peptide substrates
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
the enzyme removes N-terminal Glu, Asp, and to a lesser extent also Ser residues from peptide substrates
-
-
-
additional information
?
-
-
the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides, the enzyme is involved in metabolism of angiotensin II and angiotensin III in the brain, overview
-
-
-
additional information
?
-
-
substrate specificity, overview, the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Angiotensin II + H2O
?
-
-
-
-
-
angiotensin II + H2O
Asp + angiotensin III
angiotensin II + H2O
aspartic acid + angiotensin III
angiotensin III + H2O
angiotensin IV + Arg
cholecystokinin-8 + H2O
?
-
i.e. CCK8
-
-
?
chromogranin A + H2O
?
-
-
-
-
?
milk proteins + H2O
?
-
-
-
-
-
neurokinin B + H2O
?
-
-
-
-
?
additional information
?
-
angiotensin II + H2O

Asp + angiotensin III
-
angiotensin is the most active compound of the renin-angiotensin-system, RAS, involved in regulation of blood pressure in the brain
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
angiotensin is the most active compound of the renin-angiotensin-system, RAS, involved in renal development
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
the enzyme is responsible for the convertion of angiotensin II into angiotensin III in the brain
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
angiotensin is the most active compound of the renin-angiotensin-system, RAS, involved in renal development
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
angiotensin II is the principal effector of the renin-angiotensin-system, RAS, which induces vasoconstriction and increases sodium and water retention thereby leading to increased blood pressure
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
aspartyl aminopeptidase and several other aminopeptidases, i.e. angiotensinases, are involved in angiotensin II and cholecystokinin, CCK, metabolism, angiotensin II is the principal effector of the renin-angiotensin-system, RAS, which induces vasoconstriction and increases sodium and water retention thereby leading to increased blood pressure
-
-
?
angiotensin II + H2O

aspartic acid + angiotensin III
-
angiotensin II: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
angiotensin III: Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
angiotensin II + H2O
aspartic acid + angiotensin III
-
angiotensin II: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
angiotensin III: Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
angiotensin II + H2O
aspartic acid + angiotensin III
-
angiotensin II: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
angiotensin III: Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
angiotensin III + H2O

angiotensin IV + Arg
-
the enzyme is involved in regulation of blood pressure in the renin-angiotensin system in the brain causing hypertension
-
-
ir
angiotensin III + H2O
angiotensin IV + Arg
-
the enzyme is involved in regulation of blood pressure in the renin-angiotensin system in the brain causing hypertension
-
-
ir
angiotensin III + H2O
angiotensin IV + Arg
-
the enzyme is involved in regulation of blood pressure in the renin-angiotensin system in the brain causing hypertension
-
-
ir
angiotensin III + H2O
angiotensin IV + Arg
-
the enzyme is involved in regulation of blood pressure in the renin-angiotensin system in the brain causing hypertension
-
-
ir
additional information

?
-
-
the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides, the enzyme is involved in metabolism of angiotensin II and angiotensin III in the brain, overview
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
association between elevated BP-1 expression and cell growth. The enzyme is not involved in immune system development
-
-
-
additional information
?
-
-
The C-terminal domain of aminopeptidase A is an intramolecular chaperone required for the correct folding, cell surface expression, and activity of the enzyme
-
-
-
additional information
?
-
-
the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides, the enzyme is involved in metabolism of angiotensin II and angiotensin III in the brain, overview
-
-
-
additional information
?
-
-
the enzyme is involved in angiotensin metabolism
-
-
-
additional information
?
-
-
the enzyme is involved in conversion of angiotensin II to angiotensin III and thus in controlling of vasopressin release and blood pressure, overview
-
-
-
additional information
?
-
-
administration of purified enzyme into hypertensive rats leads to a decrease in blood pressure, while enzyme inhibition increases blood pressure
-
-
-
additional information
?
-
-
the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides, the enzyme is involved in metabolism of angiotensin II and angiotensin III in the brain, overview
-
-
-
additional information
?
-
-
the enzyme is involved in conversion of angiotensin II to angiotensin III and thus in controlling of vasopressin release and blood pressure, overview
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
potential biological functions, overview
-
-
-
additional information
?
-
-
the enzyme catalyses the hydrolysis of N-terminal glutamic or aspartic acid residues from regulatory peptides, the enzyme is involved in metabolism of angiotensin II and angiotensin III in the brain, overview
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(1-aminopropane-1,3-diyl)bis(phosphonic acid)
Q8I2J3;
-
(3R)-3-amino-3-phosphonopropanoic acid
Q8I2J3;
-
(R)-3-amino-4-mercaptobutane-1-sulfonic acid
-
i.e. EC33
(S)-3-amino-4-mercapto-butylsulfonic acid
(S)-3-amino-4-mercaptobutyl sulfonic acid
2-amino-4-methylsulfonyl butane thiol
3-amino-3-[hydroxy(phenyl)phosphoryl]propanoic acid
Q8I2J3;
-
3-amino-4-thio-butyl sulfonate
-
-
3-amino-4-thio-butyl sulfonic acid
-
EC33, specific and selective APA inhibitor
3-[(1-amino-2-carboxyethyl)(hydroxy)phosphoryl]-2-methylpropanoic acid
Q8I2J3;
-
3-[[amino(carboxy)methyl](hydroxy)phosphoryl]-2-methylpropanoic acid
Q8I2J3;
-
4,4'-dithio [bis[(3S)-3-aminobutyl sulfonic acid]]
-
i.e. RB150
4,4'-dithio[bis(3)-aminobutylsulfonic acid]
4,4'-dithio[bis(3-aminobutyl sulfonic acid)]
-
RB150, a prodrug of EC33
5-amino-5-phosphonopentanoic acid
Q8I2J3;
-
Ala-PSI[PO2-CH2]-Leu-Ala
-
-
amastin
-
3% residual activity at a concentration of 0.001 mM
-
amino(phosphono)acetic acid
Q8I2J3;
-
amino[hydroxy(methyl)phosphoryl]acetic acid
Q8I2J3;
-
angiotensin II
-
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.065 mM in presence of Ca2+
Angiotensin III
-
competitive against Glu-4-methylcoumaryl-7-amide
aspartate hydroxamate
-
-
cholecystokinin-8
-
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.024 mM in presence of Ca2+
chromogranin A
-
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.049 mM in presence of Ca2+
-
D-glutamate phosphonic acid
-
-
diisopropylfluorophosphate
-
-
DTT
-
32% activity at 0.1 mM
E64
-
slight inhibition at 1 mM
Glu-PSI[PO2-CH2]-Leu-Ala
-
-
Glu-PSI[PO2-CH2]-Leu-Arg
-
-
Glu-PSI[PO2-CH2]-Leu-Asp
-
-
Glu-PSI[PO2-CH2]-Leu-Leu
-
-
glutamate phosphonic acid
-
-
kallidin
-
competitive against Glu-4-methylcoumaryl-7-amide
L-alpha-glutaryl-L-phenylalanine
-
-
L-glutamate phosphinic acid
L-glutamate phosphonic acid
-
-
leupeptin
-
slight inhibition at 1 mM
Mg2+
-
inhibits the activity with Glu-substrate moderately
N-(2-aminoethyl)-4-methyl-5-sulfanyl-pentanamide
-
-
neurokinin B
-
competitive against Glu-4-methylcoumaryl-7-amide,IC50 is 0.060 mM in presence of Ca2+
p-hydroxymercuribenzoate
-
-
parathyroid hormone residues 69-84
-
-
-
phenylmethylsulfonyl fluoride
-
-
ZnCl2
-
0% residual activity at a concentration of 1 mM
[Ag2(E-6-(hydroxyimino)ethyl-1,3,7-trimethyl-pteridine-2,4(1H,3H)-dione)2(CF3SO3)2(EtOH)]n x nEtOH
-
compound shows cytotoxicity against both U373-MG abd NB-69 cell lines. NB-69 cells need higher concentrations of silver complexes than U373-MG cells to obtain a 50% growth inhibition. All compounds tested show apoptotic effects, with U373-MG cells being more susceptible. The silver complexes tested also show a dose-dependent inhibitory effect on aminopeptidase A activity in NB-69 and U373-MG cells, although U373-MG cells are more sensitive
-
[Ag2(E-6-(hydroxyimino)ethyl-1,3,7-trimethyl-pteridine-2,4(1H,3H)-dione)2(ClO4)2]n
-
compound shows cytotoxicity against both U373-MG abd NB-69 cell lines. NB-69 cells need higher concentrations of silver complexes than U373-MG cells to obtain a 50% growth inhibition. All compounds tested show apoptotic effects, with U373-MG cells being more susceptible. The silver complexes tested also show a dose-dependent inhibitory effect on aminopeptidase A activity in NB-69 and U373-MG cells, although U373-MG cells are more sensitive
-
[Ag2(E-6-(hydroxyimino)ethyl-1,3,7-trimethyl-pteridine-2,4(1H,3H)-dione)2(NO3)2]n
-
compound shows cytotoxicity against both U373-MG abd NB-69 cell lines. NB-69 cells need higher concentrations of silver complexes than U373-MG cells to obtain a 50% growth inhibition. All compounds tested show apoptotic effects, with U373-MG cells being more susceptible. The silver complexes tested also show a dose-dependent inhibitory effect on aminopeptidase A activity in NB-69 and U373-MG cells, although U373-MG cells are more sensitive
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-D-Ile-Asp-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-3-sulfoalanine-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-D-Asp-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-NH2
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-Asp-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-homosulfoalanine-OH
-
-
[[(2R,3R)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
[[(2R,3R)-3-amino-5-phosphonate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
[[(2R,3S)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
-
[[(2R,3S)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentane]-Ile-Asp-OH
-
-
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentanoyl]-Ile-Asp-OH
-
-
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentanoyl]-Tyr-Asp-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-D-Ile-Asp-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-3-sulfoalanine-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-D-Asp-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-NH2
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-Asp-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-homosulfoalanine-OH
-
-
[[(2S,3R)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
[[(2S,3R)-3-amino-5-phosphonate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
[[(2S,3S)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
-
[[(2S,3S)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
additional information
-
inhibitory effects of peptide hormones on enzyme activity in presence or absence of Ca2+, overview, no or poor inhibition by 4-(amidinophenyl)-methanesulfonyl fluoride, pepstatin, and bestatin
-
(S)-3-amino-4-mercapto-butylsulfonic acid

-
specific and selective aminopeptidase A inhibitor
(S)-3-amino-4-mercapto-butylsulfonic acid
-
specific and selective APA inhibitor
(S)-3-amino-4-mercaptobutyl sulfonic acid

-
EC33, specific and selective APA inhibitor
(S)-3-amino-4-mercaptobutyl sulfonic acid
-
EC33, specific and selective APA inhibitor
1,10-phenanthroline

-
-
1,10-phenanthroline
-
0% residual activity at a concentration of 1 mM
1,10-phenanthroline
-
IC50 is 0.06 mM
1,10-phenanthroline
-
reactivation by Co2+ or Zn2+
2-amino-4-methylsulfonyl butane thiol

-
weak inhibitor of aminopeptidase A
2-amino-4-methylsulfonyl butane thiol
-
weak inhibitor of APA
4,4'-dithio[bis(3)-aminobutylsulfonic acid]

-
RB150, a prodrug of EC33
4,4'-dithio[bis(3)-aminobutylsulfonic acid]
-
RB150, a prodrug of EC33
amastatin

-
-
amastatin
-
stereoisomers and analogues
amastatin
-
inhibits the enzyme and blocks the metabolism of brain angiotensin II and III
amastatin
-
IC50 is 157 nM
amastatin
-
inhibits the enzyme and blocks the metabolism of brain angiotensin II and III
amastatin
-
inhibits APA and APN
amastatin
-
inhibits the enzyme and blocks the metabolism of brain angiotensin II and III
amastatin
-
an APA inhibitor, enzyme inhibition leads to increased blood pressure
amastatin
-
inhibits APA and APN
amastatin
-
inhibits the enzyme and blocks the metabolism of brain angiotensin II and III
angiotensin IV

-
competitive feedback inhibition, negative regulatory function in absence or presence of Ca2+; IC50 is 0.010 mM
bestatin

-
38% residual activity at a concentration of 0.03 mM
bestatin
-
inhibits the enzyme and blocks the metabolism of brain angiotensin II and III
bestatin
-
inhibits the enzyme and blocks the metabolism of brain angiotensin II and III
bestatin
-
broad specificity for various aminopeptidases
bestatin
1 mM, 13% residual activity
bestatin
-
inhibits the enzyme and blocks the metabolism of brain angiotensin II and III
bestatin
-
broad specificity for various aminopeptidases
bestatin
-
inhibits the enzyme and blocks the metabolism of brain angiotensin II and III
Ca2+

-
substrate-specific inhibition, overview
Ca2+
-
inhibits activity with Gln-substrate, regulatory function and influence on substrate specificity
Ca2+
-
Ca2+ up- or down-regulates the enzyme activity depending on the substrate tested
Ca2+
-
substrate-specific inhibition, overview
Ca2+
-
Ca2+ up- or down-regulates the enzyme activity depending on the substrate tested
Ca2+
-
substrate-specific inhibition, overview
Ca2+
-
Ca2+ up- or down-regulates the enzyme activity depending on the substrate tested
Ca2+
-
substrate-specific inhibition, overview
captopril

-
-
Cd2+

-
complete inhibition at 1 mM
Cd2+
-
complete inhibition at 1 mM
Cd2+
-
complete inhibition at 1 mM
Cu2+

-
complete inhibition at 1 mM
Cu2+
-
complete inhibition at 1 mM
Cu2+
5 mM, 3.5% residual activiy
Cu2+
-
complete inhibition at 1 mM
dithiothreitol

-
-
EC33

-
specific inhibition
EC33
-
highly selective APA inhibitor
EC33
-
highly selective APA inhibitor
EC33
-
specific inhibition
EC33
-
highly selective APA inhibitor
EC33
-
specific inhibition
EC33
-
highly selective APA inhibitor
EDTA

-
-
EDTA
-
7% residual activity at a concentration of 5 mM
EDTA
-
83.5% activity at 5 mM
EDTA
-
reactivation by Co2+
EDTA
-
reactivation by Co2+
EDTA
10 mM, 3.7% residual activiy
EDTA
Q8I2J3;
activity is reduced to 14% after incubating with 10 mM EDTA
EGTA

-
-
glutamate phosphonate

-
0.001 mM, activity of wild-type enzyme is completely abolished
glutamate phosphonate
-
specific aminopeptidase A inhibitor
glutamate phosphonate
-
-
Hg2+

-
complete inhibition at 1 mM
Hg2+
-
complete inhibition at 1 mM
Hg2+
-
complete inhibition at 1 mM
L-glutamate phosphinic acid

-
-
L-glutamate phosphinic acid
-
-
methionine thiol

-
-
Mn2+

-
inhibits the activity with Glu-substrate moderately
Mn2+
5 mM, 15% residual activiy
Ni2+

-
complete inhibition at 1 mM
Ni2+
-
complete inhibition at 1 mM
Ni2+
-
complete inhibition at 1 mM
o-phenanthroline

-
47.1% activity at 2 mM
o-phenanthroline
Q8I2J3;
activity is reduced to 8.3% after incubating with 20 mM o-phenanthroline
RB150

-
selective and specific prodrug of the enzyme inhibitor EC33, inhibits the enzyme given i.v. to rats
RB150
-
selective and specific prodrug of the enzyme inhibitor EC33, inhibits the enzyme given i.v. to rats
RB150
-
selective and specific prodrug of the enzyme inhibitor EC33, inhibits the enzyme given i.v. to mice
RB150
-
selective and specific prodrug of the enzyme inhibitor EC33, inhibits the enzyme given i.v. to rats
Zn2+

-
complete inhibition at 1 mM
Zn2+
-
68.3% activity at 0.4 mM
Zn2+
-
zinc-metallopeptidase, inhibits the activity with Glu- and Gln-substrates
Zn2+
-
complete inhibition at 1 mM
Zn2+
Q8I2J3;
Zn2+ abolishes enzyme activity at a concentration of 1 mM
Zn2+
-
complete inhibition at 1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.515 - 2.307
alpha-L-Asp-2-naphthylamide
0.243 - 2.687
alpha-L-Glu-2-naphthylamide
0.1 - 5.94
alpha-L-Glu-beta-naphthylamide
0.062 - 2.85
angiotensin II
1.4 - 2.3
Asp-4-methylcoumaryl-7-amide
0.3273 - 0.3848
Asp-7-amido-4-methylcoumarin
0.72 - 0.846
Asp-beta-naphthylamide
-
maternal and normal serum
1.21 - 3.25
cholecystokinin-8
0.6 - 7.1
Gln-4-methylcoumaryl-7-amide
0.4 - 1.2
Glu-4-methylcoumaryl-7-amide
0.1351 - 4.36
Glu-7-amido-4-methylcoumarin
0.031 - 1.2
Glu-beta-naphthylamide
0.87 - 2.05
Glu-p-nitroanilide
0.159 - 2.034
L-Glu-2-naphthylamide
0.152 - 1.475
L-Glu-p-nitroanilide
additional information
additional information
-
kinetics and substrate specificity in presence or absence of Ca2+
-
0.515
alpha-L-Asp-2-naphthylamide

-
pH 7.4, 37°C, wild-type enzyme
2.272
alpha-L-Asp-2-naphthylamide
-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q
2.307
alpha-L-Asp-2-naphthylamide
-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A
0.243
alpha-L-Glu-2-naphthylamide

-
pH 7.4, 37°C, wild-type enzyme
1.943
alpha-L-Glu-2-naphthylamide
-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A
2.687
alpha-L-Glu-2-naphthylamide
-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q
0.1
alpha-L-Glu-beta-naphthylamide

-
pH 7.4, 25°C
0.16
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, wild-type enzyme
0.171
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, wild-type enzyme, in presence of 4 mM Ca2+
0.432
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, wild-type enzyme, in presence of 0.025 mM Ca2+
0.697
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, wild-type enzyme, without Ca2+
0.87
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, mutant enzyme E215Q
1.5
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, mutant enzyme E215D
2.8
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, mutant enzyme E215A
4.383
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, mutant enzyme H450F enzyme, in presence of 4 mM Ca2+
5.94
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, mutant enzyme H450F, in presence of 0.025 mM Ca2+
0.062
angiotensin II

-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A
0.073
angiotensin II
-
pH 7.4, 37°C, wild-type enzyme
0.161
angiotensin II
-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q
0.84
angiotensin II
-
wild-type, in the presence of 1.0 mM CaCl2
1.18
angiotensin II
-
mutant D221N, in the absence of CaCl2
1.22
angiotensin II
-
mutant D221N, in the presence of 1.0 mM CaCl2
2.85
angiotensin II
-
wild-type, in the absence of CaCl2
1.4
Asp-4-methylcoumaryl-7-amide

-
recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+
2.3
Asp-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+
0.3273
Asp-7-amido-4-methylcoumarin

Q8I2J3;
in the presence of 1 mM cobalt
0.3848
Asp-7-amido-4-methylcoumarin
Q8I2J3;
in the absence of cobalt
0.21
Asp-Ala

-
-
1.21
cholecystokinin-8

-
wild-type, in the presence of 1.0 mM CaCl2
2.9
cholecystokinin-8
-
mutant D221N, in the presence of 1.0 mM CaCl2
2.93
cholecystokinin-8
-
mutant D221N, in the absence of CaCl2
3.25
cholecystokinin-8
-
wild-type, in the absence of CaCl2
0.6
Gln-4-methylcoumaryl-7-amide

-
recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+
7.1
Gln-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+
0.4
Glu-4-methylcoumaryl-7-amide

-
recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+
1.2
Glu-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+
0.1351
Glu-7-amido-4-methylcoumarin

Q8I2J3;
in the absence of cobalt
0.1363
Glu-7-amido-4-methylcoumarin
Q8I2J3;
in the presence of 1 mM cobalt
0.5
Glu-7-amido-4-methylcoumarin
-
wild-type, in the presence of 1.0 mM CaCl2
1.54
Glu-7-amido-4-methylcoumarin
-
mutant D221N, in the absence of CaCl2
1.65
Glu-7-amido-4-methylcoumarin
-
mutant D221N, in the presence of 1.0 mM CaCl2
1.67
Glu-7-amido-4-methylcoumarin
-
mutant D221Q, in the absence of CaCl2
1.84
Glu-7-amido-4-methylcoumarin
-
wild-type, in the absence of CaCl2
1.95
Glu-7-amido-4-methylcoumarin
-
mutant D221Q, in the presence of 1.0 mM CaCl2
2.87
Glu-7-amido-4-methylcoumarin
-
mutant D221E, in the presence of 1.0 mM CaCl2
3.61
Glu-7-amido-4-methylcoumarin
-
mutant D221E, in the absence of CaCl2
4.31
Glu-7-amido-4-methylcoumarin
-
mutant D221A, in the presence of 1.0 mM CaCl2
4.36
Glu-7-amido-4-methylcoumarin
-
mutant D221A, in the absence of CaCl2
0.28
Glu-Ala

-
-
0.031
Glu-beta-naphthylamide

-
-
0.091 - 1.2
Glu-beta-naphthylamide
-
value depending on source of enzyme and presence of divalent cations
0.091 - 1.2
Glu-beta-naphthylamide
-
value depending on source of enzyme and presence of divalent cations
0.87
Glu-p-nitroanilide

-
ATA II
2.05
Glu-p-nitroanilide
-
ATA I
0.36
kallidin

-
wild-type, in the absence of CaCl2
0.37
kallidin
-
mutant D221N, in the absence of CaCl2
0.6
kallidin
-
mutant D221N, in the presence of 1.0 mM CaCl2
1.13
kallidin
-
wild-type, in the presence of 1.0 mM CaCl2
0.159
L-Glu-2-naphthylamide

-
recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.198
L-Glu-2-naphthylamide
-
recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.258
L-Glu-2-naphthylamide
-
recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.282
L-Glu-2-naphthylamide
-
recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
1.136
L-Glu-2-naphthylamide
-
recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
1.481
L-Glu-2-naphthylamide
-
recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
1.919
L-Glu-2-naphthylamide
-
recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
2.034
L-Glu-2-naphthylamide
-
recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.152
L-Glu-p-nitroanilide

-
wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C
1.475
L-Glu-p-nitroanilide
-
wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C
1.84
neurokinin B

-
wild-type, in the presence of 1.0 mM CaCl2
3.97
neurokinin B
-
mutant D221N, in the absence of CaCl2
4.3
neurokinin B
-
mutant D221N, in the presence of 1.0 mM CaCl2
4.94
neurokinin B
-
wild-type, in the absence of CaCl2
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
14 - 102
alpha-L-Asp-2-naphthylamide
184 - 404
alpha-L-Glu-2-naphthylamide
1.8 - 1800
alpha-L-Glu-beta-naphthylamide
2.8 - 12.2
Asp-4-methylcoumaryl-7-amide
0.05 - 0.354
Asp-7-amido-4-methylcoumarin
0.031 - 9.95
cholecystokinin-8
9.5 - 14.1
Gln-4-methylcoumaryl-7-amide
13.3 - 33.3
Glu-4-methylcoumaryl-7-amide
0.011 - 5.25
Glu-7-amido-4-methylcoumarin
2 - 640
L-Glu-2-naphthylamide
295 - 720
L-Glu-p-nitroanilide
0.052 - 10.47
neurokinin B
14
alpha-L-Asp-2-naphthylamide

-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A
25
alpha-L-Asp-2-naphthylamide
-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q
102
alpha-L-Asp-2-naphthylamide
-
pH 7.4, 37°C, wild-type enzyme
184
alpha-L-Glu-2-naphthylamide

-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q
227
alpha-L-Glu-2-naphthylamide
-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A
404
alpha-L-Glu-2-naphthylamide
-
pH 7.4, 37°C, wild-type enzyme
1.8
alpha-L-Glu-beta-naphthylamide

-
pH 7.4, 37°C, mutant enzyme H450F, in presence of 0.025 mM Ca2+
7.5
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, mutant enzyme H450F enzyme, in presence of 4 mM Ca2+
19
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, mutant enzyme E215D
48
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, mutant enzyme E215A
93
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, wild-type enzyme, without Ca2+
149
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, wild-type enzyme, in presence of 0.025 mM Ca2+
166
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, wild-type enzyme, in presence of 4 mM Ca2+
270
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, wild-type enzyme
1800
alpha-L-Glu-beta-naphthylamide
-
pH 7.4, 37°C, mutant enzyme E215Q
2.4
angiotensin II

-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353Q
2.76
angiotensin II
-
wild-type, in the absence of CaCl2
3 - 6
angiotensin II
-
wild-type, in the absence of CaCl2
3.01
angiotensin II
-
wild-type, in the presence of 1.0 mM CaCl2
3.22
angiotensin II
-
mutant D221N, in the presence of 1.0 mM CaCl2
3.88
angiotensin II
-
mutant D221N, in the absence of CaCl2
4.8
angiotensin II
-
pH 7.4, 37°C, mutant enzyme wild-type enzyme N353A
20
angiotensin II
-
pH 7.4, 37°C, wild-type enzyme
2.8
Asp-4-methylcoumaryl-7-amide

-
recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+
12.2
Asp-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+
0.05
Asp-7-amido-4-methylcoumarin

Q8I2J3;
in the absence of cobalt
0.354
Asp-7-amido-4-methylcoumarin
Q8I2J3;
in the presence of 1 mM cobalt
0.031 - 0.51
cholecystokinin-8

-
mutant D221N, in the absence of CaCl2; wild-type, in the presence of 1.0 mM CaCl2
0.052 - 2.1
cholecystokinin-8
-
mutant D221N, in the presence of 1.0 mM CaCl2
7.71
cholecystokinin-8
-
mutant D221N, in the absence of CaCl2
8.22
cholecystokinin-8
-
wild-type, in the presence of 1.0 mM CaCl2
9.18
cholecystokinin-8
-
wild-type, in the absence of CaCl2
9.95
cholecystokinin-8
-
mutant D221N, in the presence of 1.0 mM CaCl2
9.5
Gln-4-methylcoumaryl-7-amide

-
recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+
14.1
Gln-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+
13.3
Glu-4-methylcoumaryl-7-amide

-
recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+
33.3
Glu-4-methylcoumaryl-7-amide
-
recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+
0.011
Glu-7-amido-4-methylcoumarin

Q8I2J3;
in the absence of cobalt
0.33
Glu-7-amido-4-methylcoumarin
Q8I2J3;
in the presence of 1 mM cobalt
0.58
Glu-7-amido-4-methylcoumarin
-
mutant D221A, in the presence of 1.0 mM CaCl2
0.72
Glu-7-amido-4-methylcoumarin
-
mutant D221A, in the absence of CaCl2
0.88
Glu-7-amido-4-methylcoumarin
-
mutant D221E, in the absence of CaCl2
1.23
Glu-7-amido-4-methylcoumarin
-
mutant D221E, in the presence of 1.0 mM CaCl2
2.78
Glu-7-amido-4-methylcoumarin
-
mutant D221Q, in the presence of 1.0 mM CaCl2
2.96
Glu-7-amido-4-methylcoumarin
-
mutant D221Q, in the absence of CaCl2
3.4
Glu-7-amido-4-methylcoumarin
-
mutant D221N, in the absence of CaCl2
3.41
Glu-7-amido-4-methylcoumarin
-
wild-type, in the absence of CaCl2
3.68
Glu-7-amido-4-methylcoumarin
-
mutant D221N, in the presence of 1.0 mM CaCl2
5.25
Glu-7-amido-4-methylcoumarin
-
wild-type, in the presence of 1.0 mM CaCl2
0.7
kallidin

-
wild-type, in the absence of CaCl2
0.79
kallidin
-
wild-type, in the presence of 1.0 mM CaCl2
1.33
kallidin
-
mutant D221N, in the presence of 1.0 mM CaCl2
1.55
kallidin
-
mutant D221N, in the absence of CaCl2
2 - 8
L-Glu-2-naphthylamide

-
recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
40
L-Glu-2-naphthylamide
-
recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
92
L-Glu-2-naphthylamide
-
recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
167
L-Glu-2-naphthylamide
-
recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
256
L-Glu-2-naphthylamide
-
recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
292
L-Glu-2-naphthylamide
-
recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
575
L-Glu-2-naphthylamide
-
recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
640
L-Glu-2-naphthylamide
-
recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
295
L-Glu-p-nitroanilide

-
wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C
720
L-Glu-p-nitroanilide
-
wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C
0.052 - 2.1
neurokinin B

-
mutant D221N, in the absence of CaCl2
7.81
neurokinin B
-
wild-type, in the absence of CaCl2
8.87
neurokinin B
-
wild-type, in the presence of 1.0 mM CaCl2
9.8
neurokinin B
-
mutant D221N, in the presence of 1.0 mM CaCl2
10.47
neurokinin B
-
mutant D221N, in the absence of CaCl2
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0044
(1-aminopropane-1,3-diyl)bis(phosphonic acid)
Q8I2J3;
-
0.00034
(3R)-3-amino-3-phosphonopropanoic acid
Q8I2J3;
-
0.00025
(S)-3-amino-4-mercaptobutyl sulfonic acid
-
-
2
3-amino-3-[hydroxy(phenyl)phosphoryl]propanoic acid
Q8I2J3;
-
0.0427
3-[(1-amino-2-carboxyethyl)(hydroxy)phosphoryl]-2-methylpropanoic acid
Q8I2J3;
-
0.1831
3-[[amino(carboxy)methyl](hydroxy)phosphoryl]-2-methylpropanoic acid
Q8I2J3;
-
0.105
5-amino-5-phosphonopentanoic acid
Q8I2J3;
-
0.0000009
Ala-PSI[PO2-CH2]-Leu-Ala
-
pH 7.4, 25°C
0.0000059 - 0.0001927
amastatin
0.0271
amino(phosphono)acetic acid
Q8I2J3;
-
0.395
amino[hydroxy(methyl)phosphoryl]acetic acid
Q8I2J3;
-
0.00026 - 0.001297
angiotensin IV
0.0141 - 0.0289
captopril
0.0019 - 0.016
D-glutamate phosphonic acid
0.000194
Glu-PSI[PO2-CH2]-Leu
-
pH 7.4, 25°C
0.0000008
Glu-PSI[PO2-CH2]-Leu-Ala
-
pH 7.4, 25°C
0.00003
Glu-PSI[PO2-CH2]-Leu-Arg
-
pH 7.4, 25°C
0.0000057
Glu-PSI[PO2-CH2]-Leu-Asp
-
pH 7.4, 25°C
0.000037
Glu-PSI[PO2-CH2]-Leu-Leu
-
pH 7.4, 25°C
0.000038 - 0.056
glutamate phosphonic acid
0.00038 - 0.00206
glutamate thiol
0.00012 - 0.01
glutamate-thiol
0.000462 - 0.00202
glutamine-thiol
0.000045 - 0.021
L-glutamate phosphonic acid
0.0001 - 0.00394
lysine thiol
0.00046 - 0.0199
lysine-thiol
0.00019 - 0.0084
methionine thiol
0.00007
N-(2-aminoethyl)-4-methyl-5-sulfanyl-pentanamide
-
wild type enzyme, in 50 mM Tris/HCl buffer (pH 7.4), 4 mM Ca2+, at 37°C
0.00025
[(S)-3-amino-4-mercaptobutyl sulfonic acid]
-
-
0.000292
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-D-Ile-Asp-OH
-
pH 7.3, 37°C
0.0000833
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-3-sulfoalanine-OH
-
pH 7.3, 37°C
0.000053 - 0.00047
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
0.000283
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-D-Asp-OH
-
pH 7.3, 37°C
0.0042
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-NH2
-
pH 7.3, 37°C
0.0012
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-OH
-
pH 7.3, 37°C
0.000027
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-Asp-OH
-
pH 7.3, 37°C
0.0000733
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-homosulfoalanine-OH
-
pH 7.3, 37°C
0.000267
[[(2R,3R)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37°C
0.00091
[[(2R,3R)-3-amino-5-phosphonate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37°C
0.00005
[[(2R,3S)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37°C
0.000016
[[(2R,3S)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37°C
0.000051
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentane]-Ile-Asp-OH
-
pH 7.3, 37°C
0.00008
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37°C
0.00015
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentanoyl]-Tyr-Asp-OH
-
pH 7.3, 37°C
0.0000147
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-D-Ile-Asp-OH
-
pH 7.3, 37°C
0.0000036
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-3-sulfoalanine-OH
-
pH 7.3, 37°C
0.0000032 - 0.000015
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
0.00000536
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-D-Asp-OH
-
pH 7.3, 37°C
0.000145
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-NH2
-
pH 7.3, 37°C
0.00004
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-OH
-
pH 7.3, 37°C
0.0000043
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-Asp-OH
-
pH 7.3, 37°C
0.0000093
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-homosulfoalanine-OH
-
pH 7.3, 37°C
0.00000356
[[(2S,3R)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37°C
0.000012
[[(2S,3R)-3-amino-5-phosphonate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37°C
0.00002
[[(2S,3S)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37°C
0.000013
[[(2S,3S)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37°C
additional information
additional information
-
inhibition constant of 3-amino-4-thio-butyl sulfonic acid for aminopeptidase A: 0.29 microM/L
-
0.0000059
amastatin

-
mutant D221, in the presence of 1 mM CaCl2
0.0000177
amastatin
-
mutant D221, in the absence of CaCl2
0.0000243
amastatin
-
wild-type enzyme, in the absence of CaCl2
0.0001927
amastatin
-
wild-type enzyme, in the presence of 1 mM CaCl2
0.00026
angiotensin IV

-
mutant D221, in the absence of CaCl2
0.000324
angiotensin IV
-
mutant D221, in the presence of 1 mM CaCl2
0.000386
angiotensin IV
-
wild-type enzyme, in the absence of CaCl2
0.001297
angiotensin IV
-
wild-type enzyme, in the presence of 1 mM CaCl2
0.0141
captopril

-
pH 7.4, 37°C, mutant enzyme N363Q
0.0248
captopril
-
pH 7.4, 37°C, mutant enzyme N363A
0.0289
captopril
-
pH 7.4, 37°C, wild-type enzyme
0.0019
D-glutamate phosphonic acid

-
pH 7.4, 37°C, wild-type enzyme
0.00331
D-glutamate phosphonic acid
-
pH 7.4, 37°C, wild-type enzyme
0.00489
D-glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme N363Q
0.005
D-glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme E215D
0.011
D-glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme E215A
0.0112
D-glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme N363A
0.016
D-glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme E215Q
0.000038
glutamate phosphonic acid

-
pH 7.4, 37°C, wild-type enzyme, 4 mM Ca2+
0.00061
glutamate phosphonic acid
-
pH 7.4, 37°C, wild-type enzyme, 0.025 mM Ca2+
0.00084
glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme H450F, 4 mM Ca2+
0.00287
glutamate phosphonic acid
-
pH 7.4, 37°C, wild-type enzyme, no Ca2+
0.056
glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme H450F, 0.025 mM Ca2+
0.00038
glutamate thiol

-
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00085
glutamate thiol
-
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00103
glutamate thiol
-
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.0011
glutamate thiol
-
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00194
glutamate thiol
-
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00197
glutamate thiol
-
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.002
glutamate thiol
-
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00206
glutamate thiol
-
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00012
glutamate-thiol

-
pH 7.4, 37°C, wild-type enzyme, 4 mM Ca2+
0.000147
glutamate-thiol
-
pH 7.4, 37°C, wild-type enzyme
0.000216
glutamate-thiol
-
pH 7.4, 37°C, wild-type enzyme
0.00034
glutamate-thiol
-
pH 7.4, 37°C, mutant enzyme E215Q
0.00036
glutamate-thiol
-
pH 7.4, 37°C, wild-type enzyme, 0.025 mM Ca2+
0.00137
glutamate-thiol
-
pH 7.4, 37°C, mutant enzyme N363Q
0.00167
glutamate-thiol
-
pH 7.4, 37°C, wild-type enzyme, no Ca2+
0.002
glutamate-thiol
-
pH 7.4, 37°C, mutant enzyme N363A
0.00227
glutamate-thiol
-
pH 7.4, 37°C, mutant enzyme H450F, 4 mM Ca2+
0.00524
glutamate-thiol
-
pH 7.4, 37°C, mutant enzyme H450F, 0.025 mM Ca2+
0.0072
glutamate-thiol
-
pH 7.4, 37°C, mutant enzyme E215D
0.01
glutamate-thiol
-
pH 7.4, 37°C, mutant enzyme E215A
0.000462
glutamine-thiol

-
pH 7.4, 37°C, wild-type enzyme
0.00199
glutamine-thiol
-
pH 7.4, 37°C, mutant enzyme N363A
0.00202
glutamine-thiol
-
pH 7.4, 37°C, mutant enzyme N363Q
0.000045
L-glutamate phosphonic acid

-
pH 7.4, 37°C, wild-type enzyme
0.00006
L-glutamate phosphonic acid
-
pH 7.4, 37°C, wild-type enzyme
0.000214
L-glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme N363Q
0.00026
L-glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme E215Q
0.000395
L-glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme N363A
0.012
L-glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme E215D
0.021
L-glutamate phosphonic acid
-
pH 7.4, 37°C, mutant enzyme E215A
0.0001
lysine thiol

-
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00022
lysine thiol
-
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00026
lysine thiol
-
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.0009
lysine thiol
-
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00275
lysine thiol
-
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00298
lysine thiol
-
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.0036
lysine thiol
-
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00394
lysine thiol
-
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00046
lysine-thiol

-
pH 7.4, 37°C, wild-type enzyme, Ca2+
0.00047
lysine-thiol
-
pH 7.4, 37°C, wild-type enzyme, 0.025 mM Ca2+
0.0012
lysine-thiol
-
pH 7.4, 37°C, mutant enzyme H450F, 0.025 mM Ca2+
0.0072
lysine-thiol
-
pH 7.4, 37°C, wild-type enzyme, 4 mM Ca2+
0.0199
lysine-thiol
-
pH 7.4, 37°C, mutant enzyme H450F, 4 mM Ca2+
0.00019
methionine thiol

-
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00026
methionine thiol
-
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00029
methionine thiol
-
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00058
methionine thiol
-
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+
0.00307
methionine thiol
-
mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00403
methionine thiol
-
mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.00667
methionine thiol
-
mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.0084
methionine thiol
-
wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+
0.000053
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH

-
pH 7.3, 37°C
0.00047
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37°C
0.0000032
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH

-
pH 7.3, 37°C
0.000015
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.000003
-
substrate Glu-4-methyl-coumaryl-7-amide
0.000004
-
soluble glutamyl aminopeptidase activity in the atrium of euthyroid adult male rats; soluble glutamyl aminopeptidase activity in the atrium of hyperthyroid adult male rats; soluble glutamyl aminopeptidase activity in the atrium of hypothyroid adult male rats
0.0000138
-
substrate Glu-4-methyl-coumaryl-7-amide
0.000028
-
plasma enzyme, 37°C
0.000045
-
membrane-bound glutamyl aminopeptidase activity in the atrium of hypothyroid adult male rats
0.000046
-
plasma enzyme, 37°C
0.00005
-
membrane-bound glutamyl aminopeptidase activity in the atrium of euthyroid adult male rats
0.00006
-
plasma enzyme, 20°C
0.000065
-
membrane-bound glutamyl aminopeptidase activity in the atrium of hyperthyroid adult male rats
0.000069
-
plasma enzyme, 20°C
0.00007
-
plasma enzyme, 37°C
0.000686
-
DOCA-salt rat, 30 mg/kg RB150, 3.5 h after po
0.00071
-
DOCA-salt rat, 50 mg/kg RB150, 3.5 h after po
0.000726
-
DOCA-salt rat, 15 mg/kg RB150, 3.5 h after po
0.000728
-
normotensive WKY rat, 15 mg/kg RB150, 3.5 h after po
0.000752
-
normotensive WKY rat, 3.5 h after administration of saline po
0.00077
-
normotensive Sham rat, 3.5 h after administration of saline po
0.001083
-
DOCA-salt rat, 7.5 mg/kg RB150, 3.5 h after po
0.001525
-
DOCA-salt rat, 3.5 h after administration of saline po
0.001557
-
DOCA-salt rat, 15 mg/kg RB150, 24 h after po
0.06
-
aminopeptidase A activity, cortex, aminopeptidase A-deficient mice; aminopeptidase A activity, striatum, aminopeptidase A-deficient mice
0.09
-
aminopeptidase A activity, tegmentum/colliculi, aminopeptidase A-deficient mice
0.25
-
mutant enzyme T348Y, in the absence of Ca2+
0.4
-
aminopeptidase A activity, striatum, wild-type mice
0.45
-
aminopeptidase A activity, cortex, wild-type mice
0.8
-
mutant enzyme T348D, in the absence of Ca2+
1.4
-
aminopeptidase A activity, tegmentum/colliculi, wild-type mice
1.6
-
mutant enzyme T348D, in the presence of 4 mM Ca2+
1.98
-
wild type enzyme, in the absence of Ca2+
3.05
-
mutant enzyme T348S, in the absence of Ca2+
7
-
aminopeptidase A activity depending on alcohol, lung, wild-type mice, with access to a 10% ethanol solution
7.4
-
mutant enzyme T348Y, in the presence of 4 mM Ca2+
9.88
-
1 mM Glu-p-nitranilide in 0.05 M Tris/HCl, pH 7.5
11
-
aminopeptidase A activity depending on alcohol, lung, wild-type mice, without access to a 10% ethanol solution
14.67
-
wild type enzyme, in the presence of 4 mM Ca2+
21
-
aminopeptidase A activity depending on alcohol, kidney, wild-type mice, without access to a 10% ethanol solution
26
-
aminopeptidase A activity depending on alcohol, kidney, wild-type mice, with access to a 10% ethanol solution
31.54
-
mutant enzyme T348S, in the presence of 4 mM Ca2+
66.3
-
substrate Glu-Glu, pH 7.2, 37°C
132
-
substrate Asp-4-nitroanilide, pH 7.2, 37°C
319
-
substrate Glu-4-nitroanilide, pH 7.2, 37°C
607
Q8I2J3;
Glu-7-amido-4-methylcoumarin
662.5
Q8I2J3;
Asp-7-amido-4-methylcoumarin
0.0000071

-
substrate Glu-4-methyl-coumaryl-7-amide
0.0000071
-
substrate Glu-4-methyl-coumaryl-7-amide
0.000014

-
substrate Glu-4-methyl-coumaryl-7-amide
0.000014
-
plasma enzyme, 20°C
additional information
