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Literature summary for 3.4.11.7 extracted from
- Characterization and cDNA cloning of aminopeptidase A from the venom of Gloydius blomhoffi brevicaudus (2007), Toxicon, 49, 1172-1181.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Gloydius brevicaudus |  |
| BaCl2 | 319% residual activity at a concentration of 1 mM | Gloydius brevicaudus | |
| Ca2+ | - |
Gloydius brevicaudus | |
| CaCl2 | 473% residual activity at a concentration of 1 mM | Gloydius brevicaudus | |
| Mn2+ | - |
Gloydius brevicaudus | |
| MnCl2 | 181% residual activity at a concentration of 1 mM | Gloydius brevicaudus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Gloydius brevicaudus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 0% residual activity at a concentration of 1 mM | Gloydius brevicaudus | |
| amastin | 3% residual activity at a concentration of 0.001 mM | Gloydius brevicaudus | |
| bestatin | 38% residual activity at a concentration of 0.03 mM | Gloydius brevicaudus | |
| EDTA | 7% residual activity at a concentration of 5 mM | Gloydius brevicaudus | |
| Zn2+ | - |
Gloydius brevicaudus | |
| ZnCl2 | 0% residual activity at a concentration of 1 mM | Gloydius brevicaudus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bothrops jararaca | - |
- |
- |
| Crotalus atrox | - |
- |
- |
| Gloydius blomhoffii | - |
- |
- |
| Gloydius brevicaudus | - |
- |
- |
| Protobothrops flavoviridis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Gloydius blomhoffi brevicaudus venom is centrifuged and the resulting supernatant is subjected to gel-filtration on a Sephacryl S-300 column | Gloydius brevicaudus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000003 | - |
substrate Glu-4-methyl-coumaryl-7-amide | Crotalus atrox |
| 0.0000071 | - |
substrate Glu-4-methyl-coumaryl-7-amide | Protobothrops flavoviridis |
| 0.0000071 | - |
substrate Glu-4-methyl-coumaryl-7-amide | Gloydius blomhoffii |
| 0.0000138 | - |
substrate Glu-4-methyl-coumaryl-7-amide | Bothrops jararaca |
| 0.000014 | - |
substrate Glu-4-methyl-coumaryl-7-amide | Gloydius brevicaudus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glu-4-methylcoumaryl-7-amide + H2O | - |
Crotalus atrox | Glu + 7-amino-4-methylcoumarin | - |
? | |
| Glu-7-amido-4-methylcoumarin + H2O | - |
Gloydius brevicaudus | Glu + 7-amino-4-methylcoumarin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminopeptidase A | - |
Protobothrops flavoviridis |
| aminopeptidase A | - |
Crotalus atrox |
| aminopeptidase A | - |
Bothrops jararaca |
| aminopeptidase A | - |
Gloydius blomhoffii |
| aminopeptidase A | - |
Gloydius brevicaudus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Protobothrops flavoviridis |
| 37 | - |
activity assay | Crotalus atrox |
| 37 | - |
activity assay | Bothrops jararaca |
| 37 | - |
activity assay | Gloydius blomhoffii |
| 37 | - |
activity assay | Gloydius brevicaudus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
activity assay | Protobothrops flavoviridis |
| 7.4 | - |
activity assay | Crotalus atrox |
| 7.4 | - |
activity assay | Bothrops jararaca |
| 7.4 | - |
activity assay | Gloydius blomhoffii |
| 7.4 | - |
activity assay | Gloydius brevicaudus |
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