Cloned (Comment) | Organism |
---|---|
gene APE1, DNA and amino acid sequence determination and analysis, single copy gene, expression of wild-type and mutant precursor enzymes in yeast | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | isolation and/or construction of mutant enzymes defective in autophagocytosis and vacuole biogenesis, the mutant enzyme accumulates in the Cvt vesicles of the secretory pathway in non-native conformation, overview | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | precursor protein of the enzyme | Saccharomyces cerevisiae | 5829 | - |
vacuole | the precursor enzyme is transported into the vacuole via the non-classic Cvt pathway by cytoplasmic double-membrane vesicles, which fuse with the vacuole releasing a single-membrane autophagic body into the vacuolar lumen where it is proteolytically maturated, overview | Saccharomyces cerevisiae | 5773 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | maturation of the cytosolic precursor protein to the mature enzyme in the vacuolar lumen | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant precursor enzymes | Saccharomyces cerevisiae |
Subunits | Comment | Organism |
---|---|---|
dodecamer | homododecamer | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase 1 | - |
Saccharomyces cerevisiae |
APE1 | - |
Saccharomyces cerevisiae |
pApe1p | - |
Saccharomyces cerevisiae |
vacuolar aminopeptidase 1 | - |
Saccharomyces cerevisiae |