Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Bacillus subtilis and Escherichia coli | Novosphingobium capsulatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ferrocyanide | - |
Novosphingobium capsulatum | |
iodate | - |
Novosphingobium capsulatum | |
additional information | no inhibition by EDTA and PMSF | Novosphingobium capsulatum | |
o-phenanthroline | 96% inhibition | Novosphingobium capsulatum | |
phosphate | - |
Novosphingobium capsulatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
Ala beta-naphthylamide | pH 7.5, 21°C, native enzyme | Novosphingobium capsulatum | |
14 | - |
Ala-4-nitroanilide | pH 7.5, 21°C, native enzyme | Novosphingobium capsulatum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion to culture medium | Novosphingobium capsulatum | - |
- |
additional information | not in the periplasm | Novosphingobium capsulatum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no detectable content of calcium and cobalt | Novosphingobium capsulatum | |
Zinc | 1 atom per enzyme molecule, contains the HEXXH motif | Novosphingobium capsulatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Novosphingobium capsulatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Novosphingobium capsulatum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native from culture supernatant | Novosphingobium capsulatum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Release of an N-terminal amino acid, preferably a neutral or hydrophobic one, from a polypeptide. Aminoacyl-arylamides are poor substrates | Gly-Gly bond is preferably hydrolyzed | Novosphingobium capsulatum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
105 | - |
purified native enzyme, substrate Ala-4-nitroanilide | Novosphingobium capsulatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-4-nitroanilide + H2O | best Xaa-4-nitroanilide substrate | Novosphingobium capsulatum | Ala + 4-nitroaniline | - |
? | |
Ala-4-nitroanilide + H2O | best Xaa-4-nitroanilide substrate | Novosphingobium capsulatum IFO 12533 | Ala + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Tyr-Lys-amide + H2O | - |
Novosphingobium capsulatum | Ala + Ala-Pro-Tyr-Lys-amide | - |
? | |
Ala-beta-naphthylamide + H2O | - |
Novosphingobium capsulatum | Ala + beta-naphthylamine | - |
? | |
Asp-4-nitroanilide + H2O | - |
Novosphingobium capsulatum | Asp + 4-nitroaniline | - |
? | |
Glu-4-nitroanilide + H2O | - |
Novosphingobium capsulatum | Glu + 4-nitroaniline | - |
? | |
Glu-Ala-Pro-Tyr-Lys-amide + H2O | - |
Novosphingobium capsulatum | Glu + Ala-Pro-Tyr-Lys-amide | - |
? | |
Gly-4-nitroanilide + H2O | - |
Novosphingobium capsulatum | Gly + 4-nitroaniline | - |
? | |
Gly-Ala-Pro-Tyr-Lys-amide + H2O | best pentapeptide substrate | Novosphingobium capsulatum | Gly + Ala-Pro-Tyr-Lys-amide | - |
? | |
Leu-4-nitroanilide + H2O | - |
Novosphingobium capsulatum | Leu + 4-nitroaniline | - |
? | |
Leu-4-nitroanilide + H2O | - |
Novosphingobium capsulatum IFO 12533 | Leu + 4-nitroaniline | - |
? | |
Leu-Ala-Pro-Tyr-Lys-amide + H2O | - |
Novosphingobium capsulatum | Leu + Ala-Pro-Tyr-Lys-amide | - |
? | |
leucine enkephalin + H2O | recombinant enzyme, release of Tyr, Gly, Phe and Leu | Novosphingobium capsulatum | ? | - |
? | |
Lys-4-nitroanilide + H2O | low activity | Novosphingobium capsulatum | Lys + 4-nitroaniline | - |
? | |
Lys-4-nitroanilide + H2O | low activity | Novosphingobium capsulatum IFO 12533 | Lys + 4-nitroaniline | - |
? | |
Met-4-nitroanilide + H2O | - |
Novosphingobium capsulatum | Met + 4-nitroaniline | - |
? | |
Met-4-nitroanilide + H2O | - |
Novosphingobium capsulatum IFO 12533 | Met + 4-nitroaniline | - |
? | |
additional information | no activity with 4-nitroanilides of Pro, Ile, Val, and Phe | Novosphingobium capsulatum | ? | - |
? | |
additional information | no activity with 4-nitroanilides of Pro, Ile, Val, and Phe | Novosphingobium capsulatum IFO 12533 | ? | - |
? | |
Pro-Ala-Pro-Tyr-Lys-amide + H2O | - |
Novosphingobium capsulatum | Pro + Ala-Pro-Tyr-Lys-amide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Novosphingobium capsulatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
- |
Novosphingobium capsulatum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
20 min, loss of 40% activity | Novosphingobium capsulatum |
50 | - |
20 min, complete loss of activity | Novosphingobium capsulatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
Ala-beta-naphthylamide | pH 7.5, 21°C, native enzyme | Novosphingobium capsulatum | |
126.7 | - |
Ala-4-nitroanilide | pH 7.5, 21°C, native enzyme | Novosphingobium capsulatum | |
138 | - |
Ala-4-nitroanilide | pH 7.5, 21°C, native enzyme | Novosphingobium capsulatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.4 | - |
Novosphingobium capsulatum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
phosphate | pH 7.5, 21°C | Novosphingobium capsulatum | |
4.2 | - |
ferrocyanide | pH 7.5, 21°C | Novosphingobium capsulatum | |
11 | - |
iodate | pH 7.5, 21°C | Novosphingobium capsulatum |