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Literature summary for 3.4.11.21 extracted from
- Purification, characterization and cloning of a cytosolic aspartyl aminopeptidase (1998), J. Biol. Chem., 273, 15961-15970.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bacitracin | activating at 0.2 mM | Oryctolagus cuniculus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | after preincubation total loss of activity | Oryctolagus cuniculus | |
| dithiothreitol | 88% inhibition at 1 mM | Oryctolagus cuniculus | |
| p-chloromercuribenzoate | - |
Oryctolagus cuniculus | |
| Zn2+ | 50% inhibition at 0.4 M, after preincubation total inhibition | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | 2.8 | Asp-Xaa-Xaa-Xaa | - |
Oryctolagus cuniculus | |
| 0.25 | - |
angiotensin II | - |
Oryctolagus cuniculus | |
| 2 | - |
Asp-Ala-Pro-sulfamethoxazole | - |
Oryctolagus cuniculus | |
| 2 | - |
Asp-Ala-Pro-naphthylamide | - |
Oryctolagus cuniculus | |
| 2.3 | - |
Asp-Ala-Pro-sulfamethoxazole | overexpressed enzyme | Homo sapiens | |
| 8.4 | - |
Glu-Ala-Pro-naphthylamide | - |
Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Oryctolagus cuniculus | 5829 | - |
| cytosol | - |
Rattus sp. | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Oryctolagus cuniculus |
| 440000 | - |
native gel filtration | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| angiotensin + H2O | Homo sapiens | intracellular protein and peptide metabolism | Asp + des-Asp-angiotensin | - |
? | |
| angiotensin + H2O | Oryctolagus cuniculus | intracellular protein and peptide metabolism | Asp + des-Asp-angiotensin | - |
? | |
| angiotensin + H2O | Rattus sp. | intracellular protein and peptide metabolism | Asp + des-Asp-angiotensin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Oryctolagus cuniculus | - |
rabbit | - |
| Rattus sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 722fold near homogeneity by conventional chromatography techniques | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Oryctolagus cuniculus | - |
| brain | - |
Rattus sp. | - |
| heart | - |
Rattus sp. | - |
| intestine | - |
Rattus sp. | - |
| kidney | - |
Rattus sp. | - |
| liver | - |
Rattus sp. | - |
| lung | - |
Rattus sp. | - |
| spleen | - |
Rattus sp. | - |
| testis | - |
Rattus sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.013 | 0.058 | - |
Rattus sp. |
| 0.272 | 0.332 | overexpression in Escherichia coli | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| angiotensin + H2O | intracellular protein and peptide metabolism | Homo sapiens | Asp + des-Asp-angiotensin | - |
? | |
| angiotensin + H2O | intracellular protein and peptide metabolism | Oryctolagus cuniculus | Asp + des-Asp-angiotensin | - |
? | |
| angiotensin + H2O | intracellular protein and peptide metabolism | Rattus sp. | Asp + des-Asp-angiotensin | - |
? | |
| angiotensin II + H2O | octapeptides preferred to tetrapeptides | Oryctolagus cuniculus | Asp + angiotensin III | - |
? | |
| Asp-Ala-Pro-2-naphthylamide + H2O | preferring acidic amino acids favouring aspartyl residues, requires a free alpha-amino group | Oryctolagus cuniculus | Asp + Ala-Pro-2-naphthylamide | - |
? | |
| Asp-Ala-Pro-sulfamethoxazole + H2O | - |
Homo sapiens | Asp + Ala-Pro-sulfamethoxazole | - |
? | |
| Asp-Ala-Pro-sulfamethoxazole + H2O | - |
Oryctolagus cuniculus | Asp + Ala-Pro-sulfamethoxazole | - |
? | |
| Asp-Xaa-Xaa-Xaa + H2O | neutral or hydrophobic amino acids in P1 position preferred | Oryctolagus cuniculus | Asp + Xaa-Xaa-Xaa | - |
? | |
| Glu-Ala-Pro-naphthylamide + H2O | - |
Oryctolagus cuniculus | Glu + Ala-Pro-naphthylamide | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Oryctolagus cuniculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
Zn2+ | after preincubation total inhibition | Oryctolagus cuniculus | |
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