Cloned (Comment) | Organism |
---|---|
gene pepA, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Xanthomonas oryzae pv. oryzae KACC 10331 |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, sitting drop vapour diffusion method, 10 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 1 M NaCl, 3 mM 2-mercaptoethanol, 40 mM imidazole and 20% v/v glycerol, is mixed with precipitant solution containing 0.02 M CaCl2, 0.1 M sodium acetate, pH 4.6-5.0, and 15%, 20% or 40% v/v MPD, different conditions resulting in different crystal forms, X-ray diffraction structure determination and analysis at 2.6 A resolution | Xanthomonas oryzae pv. oryzae KACC 10331 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000, recombinant His-tagged enzyme, SDS-PAGE | Xanthomonas oryzae pv. oryzae KACC 10331 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Xanthomonas oryzae pv. oryzae KACC 10331 | LAP is an exopeptidase that catalyzes the hydrolysis of leucine residues from the N-terminus of a protein or peptide | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas oryzae pv. oryzae KACC 10331 | - |
gene pepA or Xoo0834 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Xanthomonas oryzae pv. oryzae KACC 10331 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | LAP is an exopeptidase that catalyzes the hydrolysis of leucine residues from the N-terminus of a protein or peptide | Xanthomonas oryzae pv. oryzae KACC 10331 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000, recombinant His-tagged enzyme, SDS-PAGE | Xanthomonas oryzae pv. oryzae KACC 10331 |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Xanthomonas oryzae pv. oryzae KACC 10331 |
leucine aminopeptidase | - |
Xanthomonas oryzae pv. oryzae KACC 10331 |