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release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids
active site structure involving His97, Asp117, Asp179, Glu151, Glu152, and His256
-
release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids
-
-
-
-
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
-
-
-
-
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
presence of at least one active Tyr and one carboxyl group demonstrated by chemical modifications of enzyme: nitration, azotization, diethyldirocarbonate treatment, 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide treatment
-
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
mechanism. Substrate only binds to the first metal binding site
-
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
catalytic mechanism
-
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
catalytic mechanism
-
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
active site structure and mechanism
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
active site structure, reaction mechanism
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
substrate binding mechanism and structure
-
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
active site residue Glu151 is essential for activity acting as a general acid/base during the catalytic reaction, mechanism of peptide hydrolysis
-
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
active site residue Glu151 is essential for activity acting as a general acid/base during the catalytic reaction, mechanism of peptide hydrolysis
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
active site structure, reaction mechanism involving divalent metal ions, overview
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
active site structure, reaction mechanism involving the two zinc ions
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Glu354 is essential for Zn2+-binding and catalytic activity, Arg356 is involved in catalysis acting as a general base that accepts a proton and activates the water nucleophile, other active site residues are Lys270, Glu354, ASp352, Lys282, Asp275, and Asp293, mechanism
-
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
residues His345 and His378 play an important role in catalysis
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
structure-activity relationship, active site structure involving zinc-binding ligands His97, Asp117, Asp179, Glu151, Glu152, and His256
-
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Ala-Gly + H2O
Ala + Gly
-
-
-
ir
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
Arg-Phe + H2O
Arg + Phe
-
-
-
ir
casein + H2O
?
-
assay at pH 8.0, 37°C
-
-
?
Cys-Gly + H2O
L-Cys + Gly
-
preferred substrate
-
-
?
DL-Ala-Gly-Gly + H2O
DL-Ala + Gly-Gly
-
-
-
ir
DL-Leu-Gly-DL-Phe + H2O
DL-Leu + Gly-DL-Phe
-
-
-
ir
DL-Leu-Gly-Gly + H2O
DL-Leu + Gly-Gly
-
-
-
ir
Glu-Phe + H2O
Glu + Phe
-
-
-
ir
glutathione + H2O
?
-
the enzyme is involved in turnover of glutathione
-
-
?
Gly-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Gly
-
assay at pH 8.0, 37°C
-
-
?
Gly-7-amido-4-methylcoumarin + H2O
Gly + 7-amino-4-methylcoumarin
-
very weak activity
-
-
?
Ile-amide + H2O
Ile + NH3
-
-
-
ir
Ile-Phe + H2O
Ile + Phe
-
-
-
ir
L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
low activity
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Ala-L-Ala-L-Phe
-
assay at pH 8.0, 37°C
-
-
?
L-Ala-p-nitroanilide + H2O
L-Ala + p-nitroaniline
-
1% activity compared to L-Leu-p-nitroanilide
-
-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
L-Arg-p-nitroanilide + H2O
L-Arg + p-nitroaniline
-
3% activity compared to L-Leu-p-nitroanilide
-
-
?
L-arginyl 7-amido-4-carbamoylmethylcoumarin + H2O
L-arginine + 7-amino-4-carbamoylmethylcoumarin
L-Asp-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Asp
-
assay at pH 8.0, 37°C
-
-
?
L-Asp-7-amido-4-methylcoumarin + H2O
L-Asp + 7-amino-4-methylcoumarin
L-Cys-4-nitroanilide + H2O
L-Cys + 4-nitroaniline
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
-
-
-
-
?
L-cysteinyl 7-amido-4-carbamoylmethylcoumarin + H2O
L-cysteine + 7-amino-4-carbamoylmethylcoumarin
L-cystinyl-p-nitroanilide + H2O
L-cystine + p-nitroaniline
-
10% activity compared to L-Leu-p-nitroanilide
-
-
?
L-Gln-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Gln
-
assay at pH 8.0, 37°C
-
-
?
L-Glu-7-amido-4-methylcoumarin + H2O
L-Glu + 7-amino-4-methylcoumarin
-
very weak activity
-
-
?
L-Ile-7-amido-4-methylcoumarin + H2O
L-Ile + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
L-Leu-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Leu
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
L-Leu-Gly-Gly + H2O
L-Leu + Gly-Gly
L-Leu-p-nitroanilide + H2O
L-Leu + p-nitroaniline
L-leucine 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
L-leucine anilide + H2O
L-leucine + aniline
-
-
-
?
L-leucine ethyl ester + H2O
L-leucine + ethanol
-
-
-
?
L-leucine-4-anisidide + H2O
L-leucine + anisidine
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
L-leucine-p-nitroanilide + H2O
L-Leu + p-nitroaniline
-
-
-
-
?
L-leucyl 7-amido-4-carbamoylmethylcoumarin + H2O
L-leucine + 7-amino-4-carbamoylmethylcoumarin
L-leucyl-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
L-leucyl-L-leucyl-L-leucine + H2O
L-leucine + L-leucyl-L-leucine
-
-
-
?
L-Lys-4-nitroanilide + H2O
4-nitroaniline + L-Lys
-
assay at pH 8.0, 37°C
-
-
?
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
-
-
-
?
L-Lys-p-nitroanilide + H2O
L-Lys + p-nitroaniline
-
24% activity compared to L-Leu-p-nitroanilide
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Met
-
assay at pH 8.0, 37°C
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
L-methionyl 7-amido-4-carbamoylmethylcoumarin + H2O
L-methionine + 7-amino-4-carbamoylmethylcoumarin
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Phe-p-nitroanilide + H2O
L-Phe + p-nitroaniline
-
23% activity compared to L-Leu-p-nitroanilide
-
-
?
L-Pro-4-nitroanilide + H2O
L-Pro + 4-nitroaniline
-
-
-
-
?
L-Pro-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Pro
-
assay at pH 8.0, 37°C
-
-
?
L-Pro-7-amido-4-methylcoumarin + H2O
L-Pro + 7-amino-4-methylcoumarin
L-Ser-7-amido-4-methylcoumarin + H2O
L-Ser + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Thr-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Thr
-
assay at pH 8.0, 37°C
-
-
?
L-Thr-7-amido-4-methylcoumarin + H2O
L-Thr + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Trp-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Trp
-
assay at pH 8.0, 37°C
-
-
?
L-Tyr-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Tyr
-
assay at pH 8.0, 37°C
-
-
?
L-Tyr-7-amido-4-methylcoumarin + H2O
L-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Val-4-nitroanilide + H2O
L-Val + 4-nitroaniline
low activity
-
-
?
L-Val-7-amido-4-methylcoumarin + H2O
L-Val + 7-amino-4-methylcoumarin
-
very weak activity
-
-
?
L-Val-L-Phe + H2O
L-Val + L-Phe
-
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
Leu-amide + H2O
Leu + NH3
Leu-beta-naphthylamide + H2O
Leu + beta-naphthylamine
Leu-Gly + H2O
Leu + Gly
-
-
-
ir
Leu-methyl ester + H2O
Leu + methanol
-
-
-
ir
leucine-4-nitroaniline + H2O
?
-
enzyme also shows activity towards Arg-4-nitroaniline, Lys-4-nitroaniline and Met-4-nitroaniline
-
-
?
Lys-4-nitroanilide + H2O
Lys + 4-nitroaniline
Lys-Gly + H2O
Lys + Gly
-
-
-
ir
Met-4-nitroanilide + H2O
Met + 4-nitroaniline
Met-amide + H2O
Met + NH3
-
-
-
ir
Met-beta-naphthylamide + H2O
Met + beta-naphthylamine
-
-
-
ir
Met-Leu-4-nitroanilide + H2O
Met-Leu + 4-nitroaniline
-
-
-
-
?
Met-Leu-7-amido-4-methylcoumarin + H2O
Met-Leu + 7-amino-4-methylcoumarin
-
-
-
-
?
Met-Phe + H2O
Met + Phe
-
-
-
ir
norleucinamide + H2O
norleucine + NH3
norvalinamide + H2O
norvaline + NH3
-
-
-
-
?
oxidized insulin B chain + H2O
?
-
assay at pH 8.0, 37°C
-
-
?
peptides + H2O
N-terminal amino acid + peptide(n-1)
-
-
-
?
Phe-amide + H2O
Phe + NH3
-
-
-
ir
Phe-Asp-Ser-Ala-Val + H2O
Phe + Asp-Ser-Ala-Val
-
-
-
ir
Phe-beta-naphthylamide + H2O
Phe + beta-naphthylamine
Phe-Gly + H2O
Phe + Gly
-
-
-
ir
Phe-methyl ester + H2O
Phe + methanol
-
-
-
ir
Phe-Phe + H2O
Phe + Phe
-
-
-
ir
Pro-Phe-Gly-Lys + H2O
Pro + Phe-Gly-Lys
-
-
-
ir
Pro-Phe-Pro + H2O
Pro + Phe-Pro
-
-
-
ir
recombinant human methionine-interferon alpha-2b + H2O
L-methionine + recombinant human interferon alpha-2b
-
-
-
?
thionoleucine-4-anisidide + H2O
thionoleucine + anisidine
-
-
-
?
thionoleucine-S-anilide + H2O
thionoleucine + aniline
-
-
-
?
Thr-beta-naphthylamide + H2O
Thr + beta-naphthylamine
-
3.3% of rate of hydrolysis of Leu-beta-naphthylamide
-
ir
Tyr-Gly + H2O
Tyr + Gly
-
-
-
ir
Val-amide + H2O
Val + NH3
-
-
-
ir
Val-beta-naphthylamide + H2O
Val + beta-naphthylamine
-
-
-
ir
Val-Gly + H2O
Val + Gly
-
-
-
ir
additional information
?
-
albomycin + H2O
?
-
-
-
-
?
albomycin + H2O
?
-
PepA activates albomycin
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
74.5% activity compared to Leu-4-nitroanilide
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
74.5% activity compared to Leu-4-nitroanilide
-
-
?
Cys-Gly + H2O
Cys + Gly
-
-
-
-
?
Cys-Gly + H2O
Cys + Gly
-
glutathione-derived substrate, PepA is involved in the cysteine salvage pathway
-
-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
best substrate
-
-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
-
-
-
-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
-
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
?
L-arginyl 7-amido-4-carbamoylmethylcoumarin + H2O
L-arginine + 7-amino-4-carbamoylmethylcoumarin
-
-
-
?
L-arginyl 7-amido-4-carbamoylmethylcoumarin + H2O
L-arginine + 7-amino-4-carbamoylmethylcoumarin
-
-
-
?
L-arginyl 7-amido-4-carbamoylmethylcoumarin + H2O
L-arginine + 7-amino-4-carbamoylmethylcoumarin
-
-
-
?
L-Asp-7-amido-4-methylcoumarin + H2O
L-Asp + 7-amino-4-methylcoumarin
-
very weak activity
-
-
?
L-Asp-7-amido-4-methylcoumarin + H2O
L-Asp + 7-amino-4-methylcoumarin
-
-
-
?
L-Asp-7-amido-4-methylcoumarin + H2O
L-Asp + 7-amino-4-methylcoumarin
-
-
-
?
L-Cys-4-nitroanilide + H2O
L-Cys + 4-nitroaniline
-
-
-
-
?
L-Cys-4-nitroanilide + H2O
L-Cys + 4-nitroaniline
-
-
-
-
?
L-cysteinyl 7-amido-4-carbamoylmethylcoumarin + H2O
L-cysteine + 7-amino-4-carbamoylmethylcoumarin
-
-
-
?
L-cysteinyl 7-amido-4-carbamoylmethylcoumarin + H2O
L-cysteine + 7-amino-4-carbamoylmethylcoumarin
-
-
-
?
L-cysteinyl 7-amido-4-carbamoylmethylcoumarin + H2O
L-cysteine + 7-amino-4-carbamoylmethylcoumarin
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
3.4.11.10 bacterial leucyl aminopeptidase
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