Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,6alpha-epoxy-5alpha-cholestan-3beta-ol | competitive to 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
5,6beta-epoxy-5beta-cholestan-3beta-ol | competitive to 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
6-oxocholestanol | - |
Rattus norvegicus | |
7-oxocholestanol | - |
Rattus norvegicus | |
7-oxocholesterol | - |
Rattus norvegicus | |
cholestane-3beta,5alpha,6beta-triol | product inhibition | Rattus norvegicus | |
additional information | no inhibition by isoquinoline, styrene oxide, cyclohexane oxide, benzil, cholesterol, and cholic acid | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Rattus norvegicus | |
0.0037 | - |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol | pH 7.4, 37°C, versus 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
0.0044 | - |
5,6beta-epoxy-5beta-cholestan-3beta-ol | pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | Rattus norvegicus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | Rattus norvegicus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
additional information | Rattus norvegicus | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Comment) | Organism |
---|---|
partially by microsome preparation | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00045 | - |
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus |
0.00048 | - |
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
additional information | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cholesterol-epoxide hydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
5,6beta-epoxy-5beta-cholestan-3beta-ol | pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
0.0044 | - |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol | pH 7.4, 37°C, versus 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus |