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Literature summary for 3.3.2.10 extracted from
- Development and characterization of recombinant whole cells expressing the soluble epoxide hydrolase of Danio rerio and its variant for enantioselective resolution of racemic styrene oxides (2012), J. Indust. Eng. Chem., 18, 384-391.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Danio rerio |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E88R/T102A/M368I/K398E/G412D | the variant possesses (S)-styrene oxide-preferred hydrolytic activity | Danio rerio |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.47 | - |
(R)-styrene oxide | wild type enzyme, at pH 6.0 and 10°C | Danio rerio |  |
| 7.85 | - |
(S)-styrene oxide | wild type enzyme, at pH 6.0 and 10°C | Danio rerio | |
| 7.95 | - |
(R)-styrene oxide | mutant enzyme E88R/T102A/M368I/K398E/G412D, at pH 6.0 and 10°C | Danio rerio | |
| 8.33 | - |
(S)-styrene oxide | mutant enzyme E88R/T102A/M368I/K398E/G412D, at pH 6.0 and 10°C | Danio rerio | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Danio rerio | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64800 | - |
x * 64800, SDS-PAGE | Danio rerio |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-styrene oxide + H2O | Danio rerio | - |
(R)-styrene glycol | - |
? | |
| (S)-styrene oxide + H2O | Danio rerio | the enzyme possesses (S)-styrene oxide-preferred hydrolytic activity | (S)-styrene glycol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | Q5PRC6 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-styrene oxide + H2O | - |
Danio rerio | (R)-styrene glycol | - |
? | |
| (S)-styrene oxide + H2O | the enzyme possesses (S)-styrene oxide-preferred hydrolytic activity | Danio rerio | (S)-styrene glycol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64800, SDS-PAGE | Danio rerio |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EPHX2 | - |
Danio rerio |
| SEH | - |
Danio rerio |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Danio rerio |
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