Literature summary for 3.2.2.B5 extracted from

Fujii, M.; Hata, C.; Ukita, M.; Fukushima, C.; Matsuura, C.; Kawashima-Ohya, Y.; Tomobe, K.; Kawashima, T.
Characterization of a thermostable 8-oxoguanine DNA glycosylase specific for GO/N mismatches from the thermoacidophilic archaeon Thermoplasma volcanium (2016), Archaea, 2016, 8734894 .

Search for more literature for 3.2.2.B5

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Thermoplasma volcanium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00002	-	DNA containing 8-oxoguanine	pH 7.5, 60°C	Thermoplasma volcanium

Organism

Organism	UniProt	Comment	Textmining
Thermoplasma volcanium	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Thermoplasma volcanium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA containing 8-oxoguanine + H2O	-	Thermoplasma volcanium	DNA with an abasic site + 8-oxoguanine	-	?
additional information	enzyme displays DNA glycosylase, and apurinic/apyrimidinic lyase activities on 7,8-dihydro-8-oxoguanine/N (where N is A, T, G, or C) mismatch. The enzyme cannot eliminate U from U/G or T from T/G	Thermoplasma volcanium	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 25000, SDS-PAGE, x * 26400, calculated from sequence, His-tagged protein	Thermoplasma volcanium

Synonyms

Synonyms	Comment	Organism
TVG_RS00315	-	Thermoplasma volcanium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Thermoplasma volcanium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Thermoplasma volcanium

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Release 2023.1 (January 2023)