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Literature summary for 3.2.2.B5 extracted from

  • Faucher, F.; Duclos, S.; Bandaru, V.; Wallace, S.S.; Doublie, S.
    Crystal structures of two archaeal 8-oxoguanine DNA glycosylases provide structural insight into guanine/8-oxoguanine distinction (2009), Structure, 17, 703-712.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.B5

Cloned(Commentary)

Cloned (Comment) Organism
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 Methanocaldococcus jannaschii
-
 Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor diffusion at 12°C Saccharolobus solfataricus
hanging-drop vapor diffusion at 12°C. A structural comparison with 8-oxoguanine DNA glycosylase 1 and archaeal GO glycosylase suggests that the C-terminal lysine of 8-oxoguanine DNA glycosylase 2 may play a key role in discriminating between guanine and 8-oxoguanine Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
K128Q glycosylase-deficient mutant Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA containing 8-oxoguanine + H2O Methanocaldococcus jannaschii base excision repair pathway DNA with an abasic site + 8-oxoguanine
-
 ?
DNA containing 8-oxoguanine + H2O Methanocaldococcus jannaschii DSM 2661 base excision repair pathway DNA with an abasic site + 8-oxoguanine
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 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58134
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-
Methanocaldococcus jannaschii DSM 2661 Q58134
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-
Saccharolobus solfataricus Q97ZK2
-
-
Saccharolobus solfataricus P2 Q97ZK2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing 8-oxoguanine + H2O base excision repair pathway Methanocaldococcus jannaschii DNA with an abasic site + 8-oxoguanine
-
 ?
DNA containing 8-oxoguanine + H2O DNA substrate used for glycosylase assay: 5'-TGTCAATAGCAAG-X-GGAGAAGTCAATCGTAGTCT-3' for the damage-containing strand, where X is either 8-oxoguanine or uracil, and 5'-AGACTCACGATTGACTTCTCC-Y-CTTGCTATTGACA-3' for the complementary strand, where Y is either one of the four bases. The enzyme displays little preference for the base opposite 8-oxoguanine Methanocaldococcus jannaschii DNA with an abasic site + 8-oxoguanine
-
 ?
DNA containing 8-oxoguanine + H2O base excision repair pathway Methanocaldococcus jannaschii DSM 2661 DNA with an abasic site + 8-oxoguanine
-
 ?
DNA containing 8-oxoguanine + H2O DNA substrate used for glycosylase assay: 5'-TGTCAATAGCAAG-X-GGAGAAGTCAATCGTAGTCT-3' for the damage-containing strand, where X is either 8-oxoguanine or uracil, and 5'-AGACTCACGATTGACTTCTCC-Y-CTTGCTATTGACA-3' for the complementary strand, where Y is either one of the four bases. The enzyme displays little preference for the base opposite 8-oxoguanine Methanocaldococcus jannaschii DSM 2661 DNA with an abasic site + 8-oxoguanine
-
 ?

Synonyms

Synonyms Comment Organism
8-oxoguanine DNA glycosylase bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) Saccharolobus solfataricus
MjaOgg2 bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) Methanocaldococcus jannaschii
Ogg2
-
 Saccharolobus solfataricus
SSO0904 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Methanocaldococcus jannaschii

General Information

General Information Comment Organism
physiological function base excision repair pathway Methanocaldococcus jannaschii