Application | Comment | Organism |
---|---|---|
medicine | potential target for chemotherapeutic intervention, nucleoside analogues which inhibit the nucleosidase could kill invading microorganisms by perturbing methylation processes and polyamine levels | Escherichia coli |
medicine | potential target for chemotherapeutic intervention, nucleoside analogues which inhibit the nucleosidase could kill invading microorganisms by perturbing methylation processes and polyamine levels | Klebsiella pneumoniae |
Cloned (Comment) | Organism |
---|---|
pfs gene cloned and expressed in pCRII using PCR, subcloned into pAL781 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenine | recombinant MTA/SAH'ase (rMTAN) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00045 | - |
5'-methylthioadenosine | recombinant nucleosidase | Escherichia coli | |
0.00053 | - |
5'-methylthioadenosine | native nucleosidase | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24350 | - |
estimation from pfs gene product | Escherichia coli |
25000 | 31000 | native MTA/SAH'ase | Escherichia coli |
26000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | Escherichia coli | - |
? | - |
? | |
5'-methylthioadenosine + H2O | Klebsiella pneumoniae | - |
? | - |
? | |
S-adenosyl-L-homocysteine + H2O | Escherichia coli | - |
? | - |
? | |
S-adenosyl-L-homocysteine + H2O | Klebsiella pneumoniae | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | - |
Escherichia coli | 5-methylthio-D-ribose + adenine | - |
? | |
5'-methylthioadenosine + H2O | - |
Klebsiella pneumoniae | 5-methylthio-D-ribose + adenine | - |
? | |
5'-methylthioadenosine + H2O | - |
Escherichia coli | ? | - |
? | |
5'-methylthioadenosine + H2O | - |
Klebsiella pneumoniae | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | adenine + S-ribosyl-L-homocysteine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Klebsiella pneumoniae | adenine + S-ribosyl-L-homocysteine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Klebsiella pneumoniae | ? | - |
? |