Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.15 extracted from

  • Pang, P.; Yang, Y.; Li, J.; Wang, Z.; Cao, W.; Xie, W.
    SMUG2 DNA glycosylase from Pedobacter heparinus as a new subfamily of the UDG superfamily (2017), Biochem. J., 474, 923-938 .
    View publication on PubMed
Search for more literature for 3.2.2.15

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the apoenzyme and inactive mutant G65Y Pedobacter heparinus

Protein Variants

Protein Variants Comment Organism
G65Y inactive, due to potential misalignment at the active site Pedobacter heparinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 uracil-containing double-stranded DNA G/U pairing, pH 7.5, 37°C Pedobacter heparinus
0.0006
-
 xanthine-containing double-stranded DNA C/xanthine pairing, pH 7.5, 37°C Pedobacter heparinus
0.0011
-
 uracil-containing double-stranded DNA A/U pairing, pH 7.5, 37°C Pedobacter heparinus
0.0029
-
 hypoxanthine-containing double-stranded DNA T/hypoxanthine pairing, pH 7.5, 37°C Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus C6Y1J8
-
-
Pedobacter heparinus DSM 2366 C6Y1J8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hypoxanthine-containing double-stranded DNA + H2O
-
 Pedobacter heparinus hypoxanthine + double-stranded DNA with abasic site
-
 ?
hypoxanthine-containing double-stranded DNA + H2O
-
 Pedobacter heparinus DSM 2366 hypoxanthine + double-stranded DNA with abasic site
-
 ?
additional information substrate is double-stranded DNA containing uracil or hypoxanthine/xanthine. No substrate: single-stranded DNA Pedobacter heparinus ?
-
 ?
additional information substrate is double-stranded DNA containing uracil or hypoxanthine/xanthine. No substrate: single-stranded DNA Pedobacter heparinus DSM 2366 ?
-
 ?
uracil-containing double-stranded DNA + H2O
-
 Pedobacter heparinus uracil + double-stranded DNA with abasic site
-
 ?
uracil-containing double-stranded DNA + H2O
-
 Pedobacter heparinus DSM 2366 uracil + double-stranded DNA with abasic site
-
 ?
xanthine-containing double-stranded DNA + H2O
-
 Pedobacter heparinus xanthine + double-stranded DNA with abasic site
-
 ?
xanthine-containing double-stranded DNA + H2O
-
 Pedobacter heparinus DSM 2366 xanthine + double-stranded DNA with abasic site
-
 ?

Synonyms

Synonyms Comment Organism
Phep_0752
-
 Pedobacter heparinus
SMUG2
-
 Pedobacter heparinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
 hypoxanthine-containing double-stranded DNA T/hypoxanthine pairing, pH 7.5, 37°C Pedobacter heparinus
0.026
-
 uracil-containing double-stranded DNA A/U pairing, pH 7.5, 37°C Pedobacter heparinus
0.059
-
 xanthine-containing double-stranded DNA C/xanthine pairing, pH 7.5, 37°C Pedobacter heparinus
0.57
-
 uracil-containing double-stranded DNA G/U pairing, pH 7.5, 37°C Pedobacter heparinus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2
-
 hypoxanthine-containing double-stranded DNA T/hypoxanthine pairing, pH 7.5, 37°C Pedobacter heparinus
24.2
-
 uracil-containing double-stranded DNA A/U pairing, pH 7.5, 37°C Pedobacter heparinus
96.5
-
 xanthine-containing double-stranded DNA C/xanthine pairing, pH 7.5, 37°C Pedobacter heparinus
1118
-
 uracil-containing double-stranded DNA G/U pairing, pH 7.5, 37°C Pedobacter heparinus