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Information on EC 3.2.2.15 - DNA-deoxyinosine glycosylase
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3.2.2.15 DNA-deoxyinosine glycosylaseSpecify your search results
Word Map
- 3.2.2.15
-
deamination
- cytosine
- glycosylases
- endonuclease
- deaminase
-
abasic
-
single-stranded
-
activation-induced
- mismatch
- thymine
- duplex
- ung2
-
hypermutation
-
misincorporation
-
uracil-containing
-
excises
- deoxyuridine
-
uracil-dna
- vpr
- ape1
-
extrahelical
-
base-excision
-
poxvirus
-
mispaired
- dutpase
-
apyrimidinic
-
translesion
- apobec3s
-
aid-induced
-
aid-dependent
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
uracil dna glycosylase, hypoxanthine dna glycosylase, hypoxanthine-dna glycosylase, family 5 udgb, deoxyribonucleic acid glycosylase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
deoxyribonucleic acid glycosylase
DNA(hypoxanthine)glycohydrolase
-
-
-
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family 5 UDGb
hypoxanthine DNA glycosylase
hypoxanthine-DNA-glycosylase
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-
-
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Phep_0752
SMUG2
deoxyribonucleic acid glycosylase
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-
-
-
family 5 UDGb
the enzyme is an uracil, hypoxanthine, and xanthine DNA glycosylase
family 5 UDGb
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
the enzyme is an uracil, hypoxanthine, and xanthine DNA glycosylase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hypoxanthine-containing DNA + H2O = hypoxanthine + DNA containing an abasic site
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-
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hypoxanthine-containing DNA + H2O = hypoxanthine + DNA containing an abasic site
specific enzymatic cleavage of the glycosyl bond
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of N-glycosyl bond
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-
-
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SYSTEMATIC NAME
IUBMB Comments
DNA-deoxyinosine deoxyribohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
68247-62-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
dsDNA + H2O
hypoxanthine + ?
-
efficiency of its excision is poor from Hx:T or Hx:C pair but better from an Hx:G pair
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-
?
hypoxanthine-containing DNA + H2O
hypoxanthine + DNA
-
incision activity is increased with age above 12 months of the Wistar and OXYS rats, relation to content of free radicals, overview
-
-
?
hypoxanthine-containing double-stranded DNA + H2O
hypoxanthine + double-stranded DNA with abasic site
hypoxanthine-containing oligodeoxynucleotide + H2O
hypoxanthine + oligodeoxynucleotide
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synthetic mismatched substrate, hyopxanthine-removing, incision activity
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-
?
xanthine-containing double-stranded DNA + H2O
xanthine + double-stranded DNA with abasic site
DNA + H2O
hypoxanthine + ?
-
preference for double-stranded substrates
liberated from DNA directly as free base, direct cleavage of the base-sugar bonds of dIMP residues in the polymer
?
DNA + H2O
hypoxanthine + ?
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more efficient on double-stranded than of single-stranded substrates, activity 10-15 times higher, removal of nonconventional residues from DNA
-
-
?
hypoxanthine-containing double-stranded DNA + H2O
hypoxanthine + double-stranded DNA
strongest activity on the G/I base pair but no activity detected on the C/I base pair
-
-
?
hypoxanthine-containing double-stranded DNA + H2O
hypoxanthine + double-stranded DNA
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
strongest activity on the G/I base pair but no activity detected on the C/I base pair
-
-
?
hypoxanthine-containing double-stranded DNA + H2O
hypoxanthine + double-stranded DNA with abasic site
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-
-
?
hypoxanthine-containing double-stranded DNA + H2O
hypoxanthine + double-stranded DNA with abasic site
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-
-
?
polydeoxynucleotides + H2O
hypoxanthine + ?
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synthetic oligonucleotides
cleaves the dIMP residue into free hypoxanthine and a deoxyribosephosphate residue remaining in DNA
?
uracil-containing double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
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-
-
?
uracil-containing double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
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-
-
?
xanthine-containing double-stranded DNA + H2O
xanthine + double-stranded DNA with abasic site
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-
-
?
xanthine-containing double-stranded DNA + H2O
xanthine + double-stranded DNA with abasic site
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-
-
?
additional information
?
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DNA repair by initiating a base excision-repair at a damaged or misincorporated residue
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-
?
additional information
?
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no detectable enzyme activity on double-stranded oligonucleotides containing mismatched base pairs
-
-
?
additional information
?
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DNA repair by initiating a base excision-repair at a damaged or misincorporated residue
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-
?
additional information
?
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DNA repair by initiating a base excision-repair at a damaged or misincorporated residue
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-
?
additional information
?
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substrate is double-stranded DNA containing uracil or hypoxanthine/xanthine. No substrate: single-stranded DNA
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-
?
additional information
?
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substrate is double-stranded DNA containing uracil or hypoxanthine/xanthine. No substrate: single-stranded DNA
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
hypoxanthine-containing DNA + H2O
hypoxanthine + DNA
-
incision activity is increased with age above 12 months of the Wistar and OXYS rats, relation to content of free radicals, overview
-
-
?
hypoxanthine-containing double-stranded DNA + H2O
hypoxanthine + double-stranded DNA
strongest activity on the G/I base pair but no activity detected on the C/I base pair
-
-
?
hypoxanthine-containing double-stranded DNA + H2O
hypoxanthine + double-stranded DNA
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
strongest activity on the G/I base pair but no activity detected on the C/I base pair
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Anemia, Hypochromic
Advanced loop-mediated isothermal amplification method for sensitive and specific detection of Tomato chlorosis virus using a uracil DNA glycosylase to control carry-over contamination.
Bloom Syndrome
Immunological alteration of the Bloom's syndrome uracil DNA glycosylase in Epstein-Barr virus-transformed human lymphoblastoid cells.
Bloom Syndrome
Immunological lesions in human uracil DNA glycosylase: association with Bloom syndrome.
Bloom Syndrome
Monoclonal antibodies detect conformational abnormality of uracil DNA glycosylase in Bloom's syndrome cells.
Bloom Syndrome
Purification and properties of the uracil DNA glycosylase from Bloom's syndrome.
Bloom Syndrome
Regulation of hypoxanthine DNA glycosylase in normal human and Bloom's syndrome fibroblasts.
Cardiovascular Diseases
Exercise Modulates Oxidative Stress and Inflammation in Aging and Cardiovascular Diseases.
Chickenpox
Identification and characterization of the simian varicella virus uracil DNA glycosylase.
Chickenpox
The simian varicella virus uracil DNA glycosylase and dUTPase genes are expressed in vivo, but are non-essential for replication in cell culture.
Colorectal Neoplasms
Inhibition of Human Uracil DNA Glycosylase Sensitizes a Large Fraction of Colorectal Cancer Cells to 5-Fluorodeoxyuridine and Raltitrexed but Not Fluorouracil.
Ecthyma, Contagious
Characterization of GM-CSF-inhibitory factor and Uracil DNA glycosylase encoding genes from camel pseudocowpoxvirus.
Esophageal Neoplasms
The efficacy of uracil DNA glycosylase pretreatment in amplicon-based massively parallel sequencing with DNA extracted from archived formalin-fixed paraffin-embedded esophageal cancer tissues.
Genetic Diseases, Inborn
Purification and properties of the uracil DNA glycosylase from Bloom's syndrome.
Hepatitis B
Uracil DNA glycosylase counteracts APOBEC3G-induced hypermutation of hepatitis B viral genomes: excision repair of covalently closed circular DNA.
Herpes Simplex
Association between the herpes simplex virus-1 DNA polymerase and uracil DNA glycosylase.
Herpes Simplex
Evidence that the herpes simplex virus type 1 uracil DNA glycosylase is required for efficient viral replication and latency in the murine nervous system.
Herpes Simplex
Mutations in accessory DNA replicating functions alter the relative mutation frequency of herpes simplex virus type 1 strains in cultured murine cells.
Herpes Simplex
Reconstitution of uracil DNA glycosylase initiated base excision repair in herpes simplex virus-1.
Infarction, Middle Cerebral Artery
Upregulation of mitochondrial base-excision repair capability within rat brain after brief ischemia.
Infections
Deficient uracil base excision repair leads to persistent dUMP in HIV proviruses during infection of monocytes and macrophages.
Infections
X4 and R5 HIV-1 have distinct post-entry requirements for uracil DNA glycosylase during infection of primary cells.
Lung Diseases
O(6)-methylguanine-DNA methyltransferase and uracil DNA glycosylase in human broncho-alveolar lavage cells and peripheral blood mononuclear cells from tobacco smokers and non-smokers.
Neoplasms
Genomically Incorporated 5-Fluorouracil that Escapes UNG-Initiated Base Excision Repair Blocks DNA Replication and Activates Homologous Recombination.
Neoplasms
Inhibition of Human Uracil DNA Glycosylase Sensitizes a Large Fraction of Colorectal Cancer Cells to 5-Fluorodeoxyuridine and Raltitrexed but Not Fluorouracil.
Neoplasms
Inhibition of uracil DNA glycosylase sensitizes cancer cells to 5-fluorodeoxyuridine through replication fork collapse-induced DNA damage.
Neoplasms
Loss of Uracil DNA Glycosylase Selectively Resensitizes p53-Mutant and -Deficient Cells to 5-FdU.
Neoplasms
The dumbbell probe mediated triple cascade signal amplification strategy for sensitive and specific detection of uracil DNA glycosylase activity.
Neoplasms
Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA glycosylase.
Neoplasms
Uracil DNA glycosylase (UNG) loss enhances DNA double strand break formation in human cancer cells exposed to pemetrexed.
Plasmacytoma
Mammalian mitochondrial endonuclease activities specific for ultraviolet-irradiated DNA.
Precursor Cell Lymphoblastic Leukemia-Lymphoma
[Frequently ABL kinase domain G:C?A:T mutation and uracil DNA glycosylase abnormal expression in TKI-resistant acute lymphoblastic leukemia of Chinese population].
Prostatic Neoplasms
Small interfering RNA-directed knockdown of uracil DNA glycosylase induces apoptosis and sensitizes human prostate cancer cells to genotoxic stress.
Tauopathies
Identification of non-Alzheimer's disease tauopathies-related proteins by proteomic analysis.
Tuberculosis
Substrate specificities and functional characterization of a thermo-tolerant uracil DNA glycosylase (UdgB) from Mycobacterium tuberculosis.
Tuberculosis
Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening.
Tumor Virus Infections
A poxvirus-encoded uracil DNA glycosylase is essential for virus viability.
Vaccinia
Construction of a vaccinia virus deficient in the essential DNA repair enzyme uracil DNA glycosylase by a complementing cell line.
Vaccinia
Crystallization and preliminary X-ray diffraction analysis of three recombinant mutants of Vaccinia virus uracil DNA glycosylase.
Vaccinia
Dominant host range selection of vaccinia recombinants by rescue of an essential gene.
Vaccinia
Effects of vaccinia virus uracil DNA glycosylase catalytic site and deoxyuridine triphosphatase deletion mutations individually and together on replication in active and quiescent cells and pathogenesis in mice.
Vaccinia
Evaluation of the role of the vaccinia virus uracil DNA glycosylase and A20 proteins as intrinsic components of the DNA polymerase holoenzyme.
Vaccinia
Highly efficient induction of protective immunity by a vaccinia virus vector defective in late gene expression.
Vaccinia
The vaccinia virus-encoded uracil DNA glycosylase has an essential role in viral DNA replication.
Vaccinia
Vaccinia virus uracil DNA glycosylase has an essential role in DNA synthesis that is independent of its glycosylase activity: catalytic site mutations reduce virulence but not virus replication in cultured cells.
Vaccinia
Vaccinia virus uracil DNA glycosylase interacts with the A20 protein to form a heterodimeric processivity factor for the viral DNA polymerase.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0029
hypoxanthine-containing double-stranded DNA
T/hypoxanthine pairing, pH 7.5, 37°C
-
0.0006
xanthine-containing double-stranded DNA
C/xanthine pairing, pH 7.5, 37°C
-
0.0005
uracil-containing double-stranded DNA
G/U pairing, pH 7.5, 37°C
-
0.0011
uracil-containing double-stranded DNA
A/U pairing, pH 7.5, 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.004
hypoxanthine-containing double-stranded DNA
T/hypoxanthine pairing, pH 7.5, 37°C
-
0.059
xanthine-containing double-stranded DNA
C/xanthine pairing, pH 7.5, 37°C
-
0.026
uracil-containing double-stranded DNA
A/U pairing, pH 7.5, 37°C
-
0.57
uracil-containing double-stranded DNA
G/U pairing, pH 7.5, 37°C
-
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.2
hypoxanthine-containing double-stranded DNA
T/hypoxanthine pairing, pH 7.5, 37°C
-
96.5
xanthine-containing double-stranded DNA
C/xanthine pairing, pH 7.5, 37°C
-
24.2
uracil-containing double-stranded DNA
A/U pairing, pH 7.5, 37°C
-
1118
uracil-containing double-stranded DNA
G/U pairing, pH 7.5, 37°C
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Wistar rats and OXYS rats, the latter showing inherited overgeneration of free radicals
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
normal human skin fibroblasts CRL-1222, progeroid skin fibroblasts AG-3199 and AG-6917
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
a repair enzyme involved in the removal of base damage
physiological function
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
a repair enzyme involved in the removal of base damage
-
Show AA Sequence and Transmembrane Helices (2085 entries)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30000
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
G65Y
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Karran, P.; Lindahl, T.
Hypoxanthine in deoxyribonucleic acid: generation by heat-induced hydrolysis of adenine residues and release in free form by a deoxyribonucleic acid glycosylase from calf thymus
Biochemistry
19
6005-6011
1980
Bos taurus, Escherichia coli, Homo sapiens
brenda
Karran, P.; Lindahl, T.
Enzymatic excision of free hypoxanthine from polydeoxynucleotides and DNA containing deoxyinosine monophosphate residues
J. Biol. Chem.
253
5877-5879
1978
Escherichia coli
brenda
Cool, B.L.; Sirover, M.A.
Proliferation-dependent regulation of DNA-glycosylases in progeroid cells
Mutat. Res.
237
211-220
1990
Homo sapiens
brenda
Dianov, G.; Lindahl, T.
Preferential recognition of I-T base-pairs in the initiation of excision-repair by hypoxanthine-DNA glycosylase
Nucleic Acids Res.
19
3829-3833
1991
Bos taurus, Escherichia coli, Homo sapiens
brenda
Ishchenko, A.; Sinitsyna, O.; Krysanova, Z.; Vasyunina, E.; Saparbaev, M.; Sidorkina, O.; Nevinsky, G.
Age-dependent increase of 8-oxoguanine-, hypoxanthine-, and uracil-DNA glycosylase activities in liver extracts from OXYS rats with inherited overgeneration of free radicals and Wistar rats
Med. Sci. Monit.
9
16-24
2003
Rattus norvegicus
brenda
Srinath, T.; Bharti, S.K.; Varshney, U.
Substrate specificities and functional characterization of a thermo-tolerant uracil DNA glycosylase (UdgB) from Mycobacterium tuberculosis
DNA Repair
6
1517-1528
2007
Mycobacterium tuberculosis
brenda
Xia, B.; Liu, Y.; Li, W.; Brice, A.; Dominy, B.; Cao, W.
Specificity and catalytic mechanism in family 5 uracil DNA glycosylase
J. Biol. Chem.
289
18413-18426
2014
Thermus thermophilus (Q5SJ65), Thermus thermophilus HB8 / ATCC 27634 / DSM 579 (Q5SJ65)
brenda
Pang, P.; Yang, Y.; Li, J.; Wang, Z.; Cao, W.; Xie, W.
SMUG2 DNA glycosylase from Pedobacter heparinus as a new subfamily of the UDG superfamily
Biochem. J.
474
923-938
2017
Pedobacter heparinus (C6Y1J8), Pedobacter heparinus DSM 2366 (C6Y1J8)
brenda
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