Information on EC 3.2.2.15 - DNA-deoxyinosine glycosylase

for references in articles please use BRENDA:EC3.2.2.15
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.2.15
-
RECOMMENDED NAME
GeneOntology No.
DNA-deoxyinosine glycosylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolyses DNA and polynucleotides, releasing free hypoxanthine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of N-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
DNA-deoxyinosine deoxyribohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
68247-62-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
calf
-
-
Manually annotated by BRENDA team
Wistar rats and OXYS rats, the latter showing inherited overgeneration of free radicals
-
-
Manually annotated by BRENDA team
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + H2O
?
show the reaction diagram
DNA + H2O
hypoxanthine + ?
show the reaction diagram
dsDNA + H2O
ethenocytosine + ?
show the reaction diagram
-
-
-
-
?
dsDNA + H2O
hypoxanthine + ?
show the reaction diagram
-
efficiency of its excision is poor from Hx:T or Hx:C pair but better from an Hx:G pair
-
-
?
dsDNA + H2O
uracil + ?
show the reaction diagram
-
no activity with ssDNA
-
-
?
hypoxanthine-containing DNA + H2O
hypoxanthine + DNA
show the reaction diagram
-
incision activity is increased with age above 12 months of the Wistar and OXYS rats, relation to content of free radicals, overview
-
-
?
hypoxanthine-containing double-stranded DNA + H2O
hypoxanthine + double-stranded DNA
show the reaction diagram
hypoxanthine-containing oligodeoxynucleotide + H2O
hypoxanthine + oligodeoxynucleotide
show the reaction diagram
-
synthetic mismatched substrate, hyopxanthine-removing, incision activity
-
-
?
polydeoxynucleotides + H2O
hypoxanthine + ?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA + H2O
?
show the reaction diagram
hypoxanthine-containing DNA + H2O
hypoxanthine + DNA
show the reaction diagram
-
incision activity is increased with age above 12 months of the Wistar and OXYS rats, relation to content of free radicals, overview
-
-
?
hypoxanthine-containing double-stranded DNA + H2O
hypoxanthine + double-stranded DNA
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
-
the enzyme contains Fe-S cluster
KCl
-
activity 4fold stimulated by 0.1 M KCl
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
higher concentrations, above 10 mM CaCl2
DNA
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markedly inhibited by low concentrations of native DNA
dsDNA
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AP-site containing
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KCl
-
2fold inhibited
Mg2+
-
higher concentrations, above 20 mM MgCl2
NaCl
-
70% inhibited by addition of 0.1 M NaCl
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0005
poly(dAdT)
-
-
-
0.0009
poly-dC
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
activity at different ages of both rat races
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 7.8
-
-
7.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
60
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10 min, stable below
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
-
SDS-PAGE
37000
-
SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56
-
half-life of 3 min when heated at 56°C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
fully active in presence of 1-5 mM EDTA
-
relatively sensitive to inhibition by neutral salts
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially
-
partially, preparation of mitochondria from liver
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BH214 cells
overexpression in Escherichia coli
-