Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.399 | - |
Inosine | in 50 mM HEPES (pH 7.3), at 25°C | Bacillus thuringiensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
exosporium | - |
Bacillus thuringiensis | 43592 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36200 | - |
calculated from amino acid sequence | Bacillus thuringiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | A7UHH1 | subspecies Bacillus thuringiensis kurstaki | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Bacillus thuringiensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spore | - |
Bacillus thuringiensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.32 | - |
in 50 mM HEPES, pH 7.3, with guanosine as substrate | Bacillus thuringiensis |
11.76 | - |
in 50 mM HEPES, pH 7.3, with adenosine as substrate | Bacillus thuringiensis |
57.2 | - |
in 50 mM HEPES, pH 7.3, with inosine as substrate | Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | - |
Bacillus thuringiensis | D-ribose + adenine | - |
? | |
guanosine + H2O | - |
Bacillus thuringiensis | D-ribose + guanine | - |
? | |
inosine + H2O | - |
Bacillus thuringiensis | D-ribose + hypoxanthine | - |
? | |
additional information | hydrolase activity could not be detected when uridine or cytidine is used as a substrate | Bacillus thuringiensis | ? | - |
? | |
additional information | IunH can hydrolyse inosine, adenosine and guanosine with the activity order: inosine Bacillus thuringiensis |
? |
- ? |
| |
Synonyms | Comment | Organism |
---|---|---|
inosine/adenosine/guanosinepreferring NH | - |
Bacillus thuringiensis |
IunH | IunH is important in moderating inosine- or adenosine-induced germination of Bacillus thuringiensis spores | Bacillus thuringiensis |
purine-specific NH | - |
Bacillus thuringiensis |
purine-specific nucleoside hydrolase | - |
Bacillus thuringiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Bacillus thuringiensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 100 | the activity is stable over a wide temperature range, the highest activity being at 80°C with high activity being maintained up to 100°C | Bacillus thuringiensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48.9 | - |
Inosine | in 50 mM HEPES (pH 7.3), at 25°C | Bacillus thuringiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus thuringiensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | inosine hydrolase activity of the purified recombinant IunH is detected over a wide range of pH values, the highest activity being obtained at pH 6 | Bacillus thuringiensis |