BRENDA - Enzyme Database
show all sequences of 3.2.1.B23

Classification of glycosidases that hydrolyze the specific positions and types of sugar moieties in ginsenosides

Shin, K.; Oh, D.; Crit. Rev. Biotechnol. 36, 1036-1049 (2016) View publication on PubMed

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ginsenoside C-K + H2O
Rhizobium sp.
-
protopanaxadiol + D-glucose
-
-
?
ginsenoside C-O + H2O
Bifidobacterium choerinum
-
ginsenoside F2 + L-arabinopyranose
-
-
?
ginsenoside G17 + H2O
Thermus thermophilus
-
ginsenoside F2 + D-glucose
-
-
?
ginsenoside G17 + H2O
Bifidobacterium longum
-
ginsenoside F2 + D-glucose
-
-
?
ginsenoside G17 + H2O
Flavobacterium johnsoniae
-
ginsenoside F2 + D-glucose
-
-
?
ginsenoside G17 + H2O
Bifidobacterium longum H-1
-
ginsenoside F2 + D-glucose
-
-
?
ginsenoside G75 + H2O
Thermus thermophilus
-
ginsenoside C-K + D-glucose
-
-
?
ginsenoside Mc + H2O
Caldicellulosiruptor saccharolyticus
-
ginsenoside C-K + L-arabinofuranose
-
-
?
ginsenoside Mc + H2O
Rhodanobacter ginsenosidimutans
-
ginsenoside C-K + L-arabinofuranose
-
-
?
ginsenoside Mc1 + H2O
Rhodanobacter ginsenosidimutans
-
ginsenoside F2 + L-arabinofuranose
-
-
?
ginsenoside Rb1 + H2O
Thermus thermophilus
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Trichoderma viride
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Aspergillus sp.
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Bifidobacterium longum
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Lissachatina fulica
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Passalora fulva
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Pseudothermotoga thermarum
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Flavobacterium johnsoniae
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Aspergillus niger
-
ginsenoside Rg3 + 2 D-glucose
-
-
?
ginsenoside Rb1 + H2O
Pseudonocardia sp.
-
ginsenoside Rg3 + 2 D-glucose
-
-
?
ginsenoside Rb1 + H2O
Microbacterium esteraromaticum
-
ginsenoside Rg3 + 2 D-glucose
-
-
?
ginsenoside Rb1 + H2O
Bifidobacterium longum H-1
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Pseudothermotoga thermarum DSM 5069T
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb2 + H2O
Trichoderma viride
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rb2 + H2O
Bifidobacterium longum
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rb2 + H2O
Bifidobacterium choerinum
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rb2 + H2O
Microbacterium esteraromaticum
-
ginsenoside Rg3 + D-glucose + L-arabinopyranose
-
-
?
ginsenoside Rb3 + H2O
Pseudonocardia sp.
-
ginsenoside Rg3 + D-glucose + D-xylose
-
-
?
ginsenoside Rc + H2O
Bifidobacterium breve
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Trichoderma viride
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Caldicellulosiruptor saccharolyticus
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Bifidobacterium longum
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Panax ginseng
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Leuconostoc sp.
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Rhodanobacter ginsenosidimutans
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Bifidobacterium longum H-1
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rd + H2O
Pseudonocardia sp.
-
ginsenoside Rg3 + D-glucose
-
-
?
ginsenoside Re + H2O
Mucilaginibacter sp. QM49
-
ginsenoside Rg2 + D-glucose
-
-
?
ginsenoside Rg1 + H2O
Mucilaginibacter sp. QM49
-
ginsenoside Rh1 + D-glucose
-
-
?
additional information
Thermus thermophilus
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Trichoderma viride
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Aspergillus sp.
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Bifidobacterium longum
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Lissachatina fulica
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Passalora fulva
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Pseudothermotoga thermarum
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Flavobacterium johnsoniae
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Bifidobacterium breve
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
?
-
-
?
additional information
Trichoderma viride
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
?
-
-
?
additional information
Caldicellulosiruptor saccharolyticus
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
?
-
-
?
additional information
Bifidobacterium longum
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
?
-
-
?
additional information
Bifidobacterium choerinum
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
?
-
-
?
additional information
Bifidobacterium longum H-1
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Bifidobacterium longum H-1
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
?
-
-
?
additional information
Pseudothermotoga thermarum DSM 5069T
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aspergillus niger
-
-
-
Aspergillus sp.
-
-
-
Bifidobacterium breve
-
-
-
Bifidobacterium choerinum
-
-
-
Bifidobacterium longum
-
-
-
Bifidobacterium longum H-1
-
-
-
Caldicellulosiruptor saccharolyticus
-
-
-
Flavobacterium johnsoniae
-
-
-
Leuconostoc sp.
-
-
-
Lissachatina fulica
-
-
-
Microbacterium esteraromaticum
-
-
-
Mucilaginibacter sp. QM49
-
-
-
Panax ginseng
-
-
-
Passalora fulva
-
-
-
Pseudonocardia sp.
-
-
-
Pseudothermotoga thermarum
-
-
-
Pseudothermotoga thermarum DSM 5069T
-
-
-
Rhizobium sp.
-
-
-
Rhodanobacter ginsenosidimutans
-
-
-
Thermus thermophilus
-
-
-
Trichoderma viride
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3
-
pH 5.0, 50°C
Panax ginseng
4
-
pH 8.0, 30°C
Mucilaginibacter sp. QM49
5
-
pH 6.5, 90°C
Thermus thermophilus
8
-
pH 6.8, 37°C
Bifidobacterium longum
9
-
pH 5.5, 37°C
Bifidobacterium breve
15
-
pH 7.5, 37°C
Rhodanobacter ginsenosidimutans
18
-
pH 6.0, 45°C
Passalora fulva
22
-
pH 5.5, 35°C
Bifidobacterium longum
24
-
pH 5.5, 45°C
Passalora fulva
26
-
pH 6.0, 37°C
Leuconostoc sp.
28
-
pH 5.5, 80°C
Caldicellulosiruptor saccharolyticus
40
-
pH 7.0, 37°C
Pseudonocardia sp.
61
-
pH 7.0, 40°C
Microbacterium esteraromaticum
67
-
pH 4.7, 37°C
Bifidobacterium longum
140
-
pH 4.8, 90°C
Pseudothermotoga thermarum
250
-
pH 5.6, 70°C
Lissachatina fulica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ginsenoside C-K + H2O
-
753334
Rhizobium sp.
protopanaxadiol + D-glucose
-
-
-
?
ginsenoside C-O + H2O
-
753334
Bifidobacterium choerinum
ginsenoside F2 + L-arabinopyranose
-
-
-
?
ginsenoside G17 + H2O
-
753334
Thermus thermophilus
ginsenoside F2 + D-glucose
-
-
-
?
ginsenoside G17 + H2O
-
753334
Bifidobacterium longum
ginsenoside F2 + D-glucose
-
-
-
?
ginsenoside G17 + H2O
-
753334
Flavobacterium johnsoniae
ginsenoside F2 + D-glucose
-
-
-
?
ginsenoside G17 + H2O
-
753334
Bifidobacterium longum H-1
ginsenoside F2 + D-glucose
-
-
-
?
ginsenoside G75 + H2O
-
753334
Thermus thermophilus
ginsenoside C-K + D-glucose
-
-
-
?
ginsenoside Mc + H2O
-
753334
Caldicellulosiruptor saccharolyticus
ginsenoside C-K + L-arabinofuranose
-
-
-
?
ginsenoside Mc + H2O
-
753334
Rhodanobacter ginsenosidimutans
ginsenoside C-K + L-arabinofuranose
-
-
-
?
ginsenoside Mc1 + H2O
-
753334
Rhodanobacter ginsenosidimutans
ginsenoside F2 + L-arabinofuranose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Thermus thermophilus
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Trichoderma viride
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Aspergillus sp.
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Bifidobacterium longum
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Lissachatina fulica
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Passalora fulva
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Pseudothermotoga thermarum
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Flavobacterium johnsoniae
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Bifidobacterium longum H-1
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Pseudothermotoga thermarum DSM 5069T
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Aspergillus niger
ginsenoside Rg3 + 2 D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Pseudonocardia sp.
ginsenoside Rg3 + 2 D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Microbacterium esteraromaticum
ginsenoside Rg3 + 2 D-glucose
-
-
-
?
ginsenoside Rb2 + H2O
-
753334
Trichoderma viride
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rb2 + H2O
-
753334
Bifidobacterium longum
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rb2 + H2O
-
753334
Bifidobacterium choerinum
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rb2 + H2O
-
753334
Microbacterium esteraromaticum
ginsenoside Rg3 + D-glucose + L-arabinopyranose
-
-
-
?
ginsenoside Rb3 + H2O
-
753334
Pseudonocardia sp.
ginsenoside Rg3 + D-glucose + D-xylose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Bifidobacterium breve
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Trichoderma viride
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Caldicellulosiruptor saccharolyticus
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Bifidobacterium longum
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Panax ginseng
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Leuconostoc sp.
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Rhodanobacter ginsenosidimutans
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Bifidobacterium longum H-1
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rd + H2O
-
753334
Pseudonocardia sp.
ginsenoside Rg3 + D-glucose
-
-
-
?
ginsenoside Re + H2O
-
753334
Mucilaginibacter sp. QM49
ginsenoside Rg2 + D-glucose
-
-
-
?
ginsenoside Rg1 + H2O
-
753334
Mucilaginibacter sp. QM49
ginsenoside Rh1 + D-glucose
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Thermus thermophilus
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Trichoderma viride
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Aspergillus sp.
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Bifidobacterium longum
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Lissachatina fulica
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Passalora fulva
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Pseudothermotoga thermarum
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Flavobacterium johnsoniae
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Bifidobacterium breve
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Trichoderma viride
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Caldicellulosiruptor saccharolyticus
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Bifidobacterium longum
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Bifidobacterium choerinum
?
-
-
-
?
additional information
hydrolysis of the C-20 inner Glc
753334
Aspergillus niger
?
-
-
-
?
additional information
hydrolysis of the C-20 inner Glc
753334
Pseudonocardia sp.
?
-
-
-
?
additional information
hydrolysis of the C-20 inner Glc
753334
Rhizobium sp.
?
-
-
-
?
additional information
hydrolysis of the C-20 inner Glc
753334
Microbacterium esteraromaticum
?
-
-
-
?
additional information
hydrolysis of the C-20 inner Glc
753334
Mucilaginibacter sp. QM49
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Bifidobacterium longum H-1
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Bifidobacterium longum H-1
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Pseudothermotoga thermarum DSM 5069T
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 56000
Rhodanobacter ginsenosidimutans
?
x * 57000
Bifidobacterium longum
?
x * 57000
Pseudothermotoga thermarum
?
x * 49000
Thermus thermophilus
?
x * 58000
Caldicellulosiruptor saccharolyticus
?
x * 58000
Leuconostoc sp.
?
x * 80000
Caldicellulosiruptor saccharolyticus
?
x * 80000
Passalora fulva
?
x * 88000
Microbacterium esteraromaticum
?
x * 60000
Aspergillus sp.
?
x * 86000
Panax ginseng
?
x * 120000
Lissachatina fulica
?
x * 77000
Bifidobacterium breve
?
x * 79000
Pseudonocardia sp.
?
x * 81000
Bifidobacterium longum
?
x * 82000
Flavobacterium johnsoniae
?
x * 85000
Mucilaginibacter sp. QM49
?
x * 87000
Microbacterium esteraromaticum
?
x * 90000
Passalora fulva
?
x * 95000
Bifidobacterium longum
Synonyms
Synonyms
Commentary
Organism
alpha-L-arabinofuranosidase
-
Caldicellulosiruptor saccharolyticus
alpha-L-arabinofuranosidase
-
Bifidobacterium longum
alpha-L-arabinofuranosidase
-
Panax ginseng
alpha-L-arabinofuranosidase
-
Leuconostoc sp.
alpha-L-arabinofuranosidase
-
Rhodanobacter ginsenosidimutans
alpha-L-arabinopyranosidase
-
Bifidobacterium breve
alpha-L-arabinopyranosidase
-
Caldicellulosiruptor saccharolyticus
alpha-L-arabinopyranosidase
-
Bifidobacterium longum
beta-galactosidase
-
Caldicellulosiruptor saccharolyticus
beta-glucosidase
-
Thermus thermophilus
beta-glucosidase
-
Pseudonocardia sp.
beta-glucosidase
-
Aspergillus sp.
beta-glucosidase
-
Microbacterium esteraromaticum
beta-glucosidase
-
Bifidobacterium longum
beta-glucosidase
-
Lissachatina fulica
beta-glucosidase
-
Pseudothermotoga thermarum
beta-glucosidase
-
Flavobacterium johnsoniae
beta-glucosidase
-
Mucilaginibacter sp. QM49
beta-glucosidase GI
-
Passalora fulva
beta-glucosidase GII
-
Passalora fulva
ginsenosidase type II
-
Thermus thermophilus
ginsenosidase type II
-
Aspergillus niger
ginsenosidase type II
-
Pseudonocardia sp.
ginsenosidase type II
-
Rhizobium sp.
ginsenosidase type II
-
Bifidobacterium breve
ginsenosidase type II
-
Trichoderma viride
ginsenosidase type II
-
Aspergillus sp.
ginsenosidase type II
-
Caldicellulosiruptor saccharolyticus
ginsenosidase type II
-
Microbacterium esteraromaticum
ginsenosidase type II
-
Bifidobacterium longum
ginsenosidase type II
-
Lissachatina fulica
ginsenosidase type II
-
Panax ginseng
ginsenosidase type II
-
Passalora fulva
ginsenosidase type II
-
Leuconostoc sp.
ginsenosidase type II
-
Pseudothermotoga thermarum
ginsenosidase type II
-
Flavobacterium johnsoniae
ginsenosidase type II
-
Bifidobacterium choerinum
ginsenosidase type II
-
Rhodanobacter ginsenosidimutans
ginsenosidase type II
-
Mucilaginibacter sp. QM49
ginsenosidase type II-i-1
-
Thermus thermophilus
ginsenosidase type II-i-1
-
Trichoderma viride
ginsenosidase type II-i-1
-
Aspergillus sp.
ginsenosidase type II-i-1
-
Bifidobacterium longum
ginsenosidase type II-i-1
-
Lissachatina fulica
ginsenosidase type II-i-1
-
Passalora fulva
ginsenosidase type II-i-1
-
Pseudothermotoga thermarum
ginsenosidase type II-i-1
-
Flavobacterium johnsoniae
ginsenosidase type II-i-2
-
Bifidobacterium breve
ginsenosidase type II-i-2
-
Trichoderma viride
ginsenosidase type II-i-2
-
Caldicellulosiruptor saccharolyticus
ginsenosidase type II-i-2
-
Bifidobacterium longum
ginsenosidase type II-i-2
-
Bifidobacterium choerinum
ginsenosidase type II-i-4
-
Trichoderma viride
ginsenosidase type II-i-4
-
Caldicellulosiruptor saccharolyticus
ginsenosidase type II-i-4
-
Bifidobacterium longum
ginsenosidase type II-i-4
-
Panax ginseng
ginsenosidase type II-i-4
-
Leuconostoc sp.
ginsenosidase type II-i-4
-
Rhodanobacter ginsenosidimutans
ginsenosidase type II-ii
-
Aspergillus niger
ginsenosidase type II-ii
-
Pseudonocardia sp.
ginsenosidase type II-ii
-
Rhizobium sp.
ginsenosidase type II-ii
-
Microbacterium esteraromaticum
ginsenosidase type II-ii
-
Mucilaginibacter sp. QM49
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Mucilaginibacter sp. QM49
35
-
-
Bifidobacterium longum
37
-
-
Pseudonocardia sp.
37
-
-
Bifidobacterium breve
37
-
-
Bifidobacterium longum
37
-
-
Leuconostoc sp.
37
-
-
Flavobacterium johnsoniae
37
-
-
Rhodanobacter ginsenosidimutans
40
-
-
Microbacterium esteraromaticum
45
-
-
Passalora fulva
50
-
-
Aspergillus sp.
50
-
-
Panax ginseng
70
-
-
Lissachatina fulica
80
-
-
Caldicellulosiruptor saccharolyticus
90
-
-
Thermus thermophilus
90
-
-
Pseudothermotoga thermarum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.7
-
-
Bifidobacterium longum
4.8
-
-
Pseudothermotoga thermarum
5
-
-
Aspergillus sp.
5
-
-
Panax ginseng
5.5
-
-
Bifidobacterium breve
5.5
-
-
Caldicellulosiruptor saccharolyticus
5.5
-
-
Bifidobacterium longum
5.5
-
-
Passalora fulva
5.6
-
-
Lissachatina fulica
6
-
-
Caldicellulosiruptor saccharolyticus
6
-
-
Passalora fulva
6
-
-
Leuconostoc sp.
6
-
-
Flavobacterium johnsoniae
6.5
-
-
Thermus thermophilus
6.8
-
-
Bifidobacterium longum
7
-
-
Pseudonocardia sp.
7
-
-
Microbacterium esteraromaticum
7.5
-
-
Rhodanobacter ginsenosidimutans
8
-
-
Mucilaginibacter sp. QM49
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ginsenoside C-K + H2O
Rhizobium sp.
-
protopanaxadiol + D-glucose
-
-
?
ginsenoside C-O + H2O
Bifidobacterium choerinum
-
ginsenoside F2 + L-arabinopyranose
-
-
?
ginsenoside G17 + H2O
Thermus thermophilus
-
ginsenoside F2 + D-glucose
-
-
?
ginsenoside G17 + H2O
Bifidobacterium longum
-
ginsenoside F2 + D-glucose
-
-
?
ginsenoside G17 + H2O
Flavobacterium johnsoniae
-
ginsenoside F2 + D-glucose
-
-
?
ginsenoside G17 + H2O
Bifidobacterium longum H-1
-
ginsenoside F2 + D-glucose
-
-
?
ginsenoside G75 + H2O
Thermus thermophilus
-
ginsenoside C-K + D-glucose
-
-
?
ginsenoside Mc + H2O
Caldicellulosiruptor saccharolyticus
-
ginsenoside C-K + L-arabinofuranose
-
-
?
ginsenoside Mc + H2O
Rhodanobacter ginsenosidimutans
-
ginsenoside C-K + L-arabinofuranose
-
-
?
ginsenoside Mc1 + H2O
Rhodanobacter ginsenosidimutans
-
ginsenoside F2 + L-arabinofuranose
-
-
?
ginsenoside Rb1 + H2O
Thermus thermophilus
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Trichoderma viride
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Aspergillus sp.
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Bifidobacterium longum
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Lissachatina fulica
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Passalora fulva
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Pseudothermotoga thermarum
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Flavobacterium johnsoniae
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Aspergillus niger
-
ginsenoside Rg3 + 2 D-glucose
-
-
?
ginsenoside Rb1 + H2O
Pseudonocardia sp.
-
ginsenoside Rg3 + 2 D-glucose
-
-
?
ginsenoside Rb1 + H2O
Microbacterium esteraromaticum
-
ginsenoside Rg3 + 2 D-glucose
-
-
?
ginsenoside Rb1 + H2O
Bifidobacterium longum H-1
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb1 + H2O
Pseudothermotoga thermarum DSM 5069T
-
ginsenoside Rd + D-glucose
-
-
?
ginsenoside Rb2 + H2O
Trichoderma viride
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rb2 + H2O
Bifidobacterium longum
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rb2 + H2O
Bifidobacterium choerinum
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rb2 + H2O
Microbacterium esteraromaticum
-
ginsenoside Rg3 + D-glucose + L-arabinopyranose
-
-
?
ginsenoside Rb3 + H2O
Pseudonocardia sp.
-
ginsenoside Rg3 + D-glucose + D-xylose
-
-
?
ginsenoside Rc + H2O
Bifidobacterium breve
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Trichoderma viride
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Caldicellulosiruptor saccharolyticus
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Bifidobacterium longum
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Panax ginseng
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Leuconostoc sp.
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Rhodanobacter ginsenosidimutans
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rc + H2O
Bifidobacterium longum H-1
-
ginsenoside Rd + L-arabinofuranose
-
-
?
ginsenoside Rd + H2O
Pseudonocardia sp.
-
ginsenoside Rg3 + D-glucose
-
-
?
ginsenoside Re + H2O
Mucilaginibacter sp. QM49
-
ginsenoside Rg2 + D-glucose
-
-
?
ginsenoside Rg1 + H2O
Mucilaginibacter sp. QM49
-
ginsenoside Rh1 + D-glucose
-
-
?
additional information
Thermus thermophilus
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Trichoderma viride
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Aspergillus sp.
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Bifidobacterium longum
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
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-
-
?
additional information
Lissachatina fulica
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Passalora fulva
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
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?
additional information
Pseudothermotoga thermarum
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
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-
?
additional information
Flavobacterium johnsoniae
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
?
-
-
?
additional information
Bifidobacterium breve
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
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?
additional information
Trichoderma viride
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
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?
additional information
Caldicellulosiruptor saccharolyticus
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
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-
?
additional information
Bifidobacterium longum
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
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?
additional information
Bifidobacterium choerinum
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
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?
additional information
Bifidobacterium longum H-1
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
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?
additional information
Bifidobacterium longum H-1
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
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additional information
Pseudothermotoga thermarum DSM 5069T
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
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Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3
-
pH 5.0, 50°C
Panax ginseng
4
-
pH 8.0, 30°C
Mucilaginibacter sp. QM49
5
-
pH 6.5, 90°C
Thermus thermophilus
8
-
pH 6.8, 37°C
Bifidobacterium longum
9
-
pH 5.5, 37°C
Bifidobacterium breve
15
-
pH 7.5, 37°C
Rhodanobacter ginsenosidimutans
18
-
pH 6.0, 45°C
Passalora fulva
22
-
pH 5.5, 35°C
Bifidobacterium longum
24
-
pH 5.5, 45°C
Passalora fulva
26
-
pH 6.0, 37°C
Leuconostoc sp.
28
-
pH 5.5, 80°C
Caldicellulosiruptor saccharolyticus
40
-
pH 7.0, 37°C
Pseudonocardia sp.
61
-
pH 7.0, 40°C
Microbacterium esteraromaticum
67
-
pH 4.7, 37°C
Bifidobacterium longum
140
-
pH 4.8, 90°C
Pseudothermotoga thermarum
250
-
pH 5.6, 70°C
Lissachatina fulica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ginsenoside C-K + H2O
-
753334
Rhizobium sp.
protopanaxadiol + D-glucose
-
-
-
?
ginsenoside C-O + H2O
-
753334
Bifidobacterium choerinum
ginsenoside F2 + L-arabinopyranose
-
-
-
?
ginsenoside G17 + H2O
-
753334
Thermus thermophilus
ginsenoside F2 + D-glucose
-
-
-
?
ginsenoside G17 + H2O
-
753334
Bifidobacterium longum
ginsenoside F2 + D-glucose
-
-
-
?
ginsenoside G17 + H2O
-
753334
Flavobacterium johnsoniae
ginsenoside F2 + D-glucose
-
-
-
?
ginsenoside G17 + H2O
-
753334
Bifidobacterium longum H-1
ginsenoside F2 + D-glucose
-
-
-
?
ginsenoside G75 + H2O
-
753334
Thermus thermophilus
ginsenoside C-K + D-glucose
-
-
-
?
ginsenoside Mc + H2O
-
753334
Caldicellulosiruptor saccharolyticus
ginsenoside C-K + L-arabinofuranose
-
-
-
?
ginsenoside Mc + H2O
-
753334
Rhodanobacter ginsenosidimutans
ginsenoside C-K + L-arabinofuranose
-
-
-
?
ginsenoside Mc1 + H2O
-
753334
Rhodanobacter ginsenosidimutans
ginsenoside F2 + L-arabinofuranose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Thermus thermophilus
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Trichoderma viride
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Aspergillus sp.
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Bifidobacterium longum
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Lissachatina fulica
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Passalora fulva
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Pseudothermotoga thermarum
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Flavobacterium johnsoniae
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Bifidobacterium longum H-1
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Pseudothermotoga thermarum DSM 5069T
ginsenoside Rd + D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Aspergillus niger
ginsenoside Rg3 + 2 D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Pseudonocardia sp.
ginsenoside Rg3 + 2 D-glucose
-
-
-
?
ginsenoside Rb1 + H2O
-
753334
Microbacterium esteraromaticum
ginsenoside Rg3 + 2 D-glucose
-
-
-
?
ginsenoside Rb2 + H2O
-
753334
Trichoderma viride
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rb2 + H2O
-
753334
Bifidobacterium longum
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rb2 + H2O
-
753334
Bifidobacterium choerinum
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rb2 + H2O
-
753334
Microbacterium esteraromaticum
ginsenoside Rg3 + D-glucose + L-arabinopyranose
-
-
-
?
ginsenoside Rb3 + H2O
-
753334
Pseudonocardia sp.
ginsenoside Rg3 + D-glucose + D-xylose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Bifidobacterium breve
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Trichoderma viride
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Caldicellulosiruptor saccharolyticus
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Bifidobacterium longum
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Panax ginseng
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Leuconostoc sp.
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Rhodanobacter ginsenosidimutans
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rc + H2O
-
753334
Bifidobacterium longum H-1
ginsenoside Rd + L-arabinofuranose
-
-
-
?
ginsenoside Rd + H2O
-
753334
Pseudonocardia sp.
ginsenoside Rg3 + D-glucose
-
-
-
?
ginsenoside Re + H2O
-
753334
Mucilaginibacter sp. QM49
ginsenoside Rg2 + D-glucose
-
-
-
?
ginsenoside Rg1 + H2O
-
753334
Mucilaginibacter sp. QM49
ginsenoside Rh1 + D-glucose
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Thermus thermophilus
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Trichoderma viride
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Aspergillus sp.
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Bifidobacterium longum
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Lissachatina fulica
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Passalora fulva
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Pseudothermotoga thermarum
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Flavobacterium johnsoniae
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Bifidobacterium breve
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Trichoderma viride
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Caldicellulosiruptor saccharolyticus
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Bifidobacterium longum
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Bifidobacterium choerinum
?
-
-
-
?
additional information
hydrolysis of the C-20 inner Glc
753334
Aspergillus niger
?
-
-
-
?
additional information
hydrolysis of the C-20 inner Glc
753334
Pseudonocardia sp.
?
-
-
-
?
additional information
hydrolysis of the C-20 inner Glc
753334
Rhizobium sp.
?
-
-
-
?
additional information
hydrolysis of the C-20 inner Glc
753334
Microbacterium esteraromaticum
?
-
-
-
?
additional information
hydrolysis of the C-20 inner Glc
753334
Mucilaginibacter sp. QM49
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Bifidobacterium longum H-1
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
753334
Bifidobacterium longum H-1
?
-
-
-
?
additional information
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
753334
Pseudothermotoga thermarum DSM 5069T
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 56000
Rhodanobacter ginsenosidimutans
?
x * 57000
Bifidobacterium longum
?
x * 57000
Pseudothermotoga thermarum
?
x * 49000
Thermus thermophilus
?
x * 58000
Caldicellulosiruptor saccharolyticus
?
x * 58000
Leuconostoc sp.
?
x * 80000
Caldicellulosiruptor saccharolyticus
?
x * 80000
Passalora fulva
?
x * 88000
Microbacterium esteraromaticum
?
x * 60000
Aspergillus sp.
?
x * 86000
Panax ginseng
?
x * 120000
Lissachatina fulica
?
x * 77000
Bifidobacterium breve
?
x * 79000
Pseudonocardia sp.
?
x * 81000
Bifidobacterium longum
?
x * 82000
Flavobacterium johnsoniae
?
x * 85000
Mucilaginibacter sp. QM49
?
x * 87000
Microbacterium esteraromaticum
?
x * 90000
Passalora fulva
?
x * 95000
Bifidobacterium longum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Mucilaginibacter sp. QM49
35
-
-
Bifidobacterium longum
37
-
-
Pseudonocardia sp.
37
-
-
Bifidobacterium breve
37
-
-
Bifidobacterium longum
37
-
-
Leuconostoc sp.
37
-
-
Flavobacterium johnsoniae
37
-
-
Rhodanobacter ginsenosidimutans
40
-
-
Microbacterium esteraromaticum
45
-
-
Passalora fulva
50
-
-
Aspergillus sp.
50
-
-
Panax ginseng
70
-
-
Lissachatina fulica
80
-
-
Caldicellulosiruptor saccharolyticus
90
-
-
Thermus thermophilus
90
-
-
Pseudothermotoga thermarum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.7
-
-
Bifidobacterium longum
4.8
-
-
Pseudothermotoga thermarum
5
-
-
Aspergillus sp.
5
-
-
Panax ginseng
5.5
-
-
Bifidobacterium breve
5.5
-
-
Caldicellulosiruptor saccharolyticus
5.5
-
-
Bifidobacterium longum
5.5
-
-
Passalora fulva
5.6
-
-
Lissachatina fulica
6
-
-
Caldicellulosiruptor saccharolyticus
6
-
-
Passalora fulva
6
-
-
Leuconostoc sp.
6
-
-
Flavobacterium johnsoniae
6.5
-
-
Thermus thermophilus
6.8
-
-
Bifidobacterium longum
7
-
-
Pseudonocardia sp.
7
-
-
Microbacterium esteraromaticum
7.5
-
-
Rhodanobacter ginsenosidimutans
8
-
-
Mucilaginibacter sp. QM49
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the glycosyl hydrolase family 1, GH1
Thermus thermophilus
evolution
the enzyme belongs to the glycosyl hydrolase family 1, GH1
Pseudothermotoga thermarum
evolution
the enzyme belongs to the glycosyl hydrolase family 2, GH2
Microbacterium esteraromaticum
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3
Pseudonocardia sp.
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3
Microbacterium esteraromaticum
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3
Bifidobacterium longum
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3
Flavobacterium johnsoniae
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3
Mucilaginibacter sp. QM49
evolution
the enzyme belongs to the glycosyl hydrolase family 42, GH42
Caldicellulosiruptor saccharolyticus
evolution
the enzyme belongs to the glycosyl hydrolase family 51, GH51
Caldicellulosiruptor saccharolyticus
evolution
the enzyme belongs to the glycosyl hydrolase family 51, GH51
Bifidobacterium longum
evolution
the enzyme belongs to the glycosyl hydrolase family 51, GH51
Leuconostoc sp.
evolution
the enzyme belongs to the glycosyl hydrolase family 51, GH51
Rhodanobacter ginsenosidimutans
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Trichoderma viride
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Caldicellulosiruptor saccharolyticus
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Bifidobacterium longum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Panax ginseng
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Leuconostoc sp.
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Rhodanobacter ginsenosidimutans
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
Bifidobacterium breve
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
Trichoderma viride
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
Caldicellulosiruptor saccharolyticus
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
Bifidobacterium longum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
Bifidobacterium choerinum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Thermus thermophilus
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Trichoderma viride
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Aspergillus sp.
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Bifidobacterium longum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Lissachatina fulica
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Passalora fulva
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Pseudothermotoga thermarum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Flavobacterium johnsoniae
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides
Aspergillus niger
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides
Pseudonocardia sp.
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides
Rhizobium sp.
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides
Microbacterium esteraromaticum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides
Mucilaginibacter sp. QM49
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the glycosyl hydrolase family 1, GH1
Thermus thermophilus
evolution
the enzyme belongs to the glycosyl hydrolase family 1, GH1
Pseudothermotoga thermarum
evolution
the enzyme belongs to the glycosyl hydrolase family 2, GH2
Microbacterium esteraromaticum
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3
Pseudonocardia sp.
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3
Microbacterium esteraromaticum
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3
Bifidobacterium longum
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3
Flavobacterium johnsoniae
evolution
the enzyme belongs to the glycosyl hydrolase family 3, GH3
Mucilaginibacter sp. QM49
evolution
the enzyme belongs to the glycosyl hydrolase family 42, GH42
Caldicellulosiruptor saccharolyticus
evolution
the enzyme belongs to the glycosyl hydrolase family 51, GH51
Caldicellulosiruptor saccharolyticus
evolution
the enzyme belongs to the glycosyl hydrolase family 51, GH51
Bifidobacterium longum
evolution
the enzyme belongs to the glycosyl hydrolase family 51, GH51
Leuconostoc sp.
evolution
the enzyme belongs to the glycosyl hydrolase family 51, GH51
Rhodanobacter ginsenosidimutans
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Trichoderma viride
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Caldicellulosiruptor saccharolyticus
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Bifidobacterium longum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Panax ginseng
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Leuconostoc sp.
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2
Rhodanobacter ginsenosidimutans
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
Bifidobacterium breve
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
Trichoderma viride
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
Caldicellulosiruptor saccharolyticus
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
Bifidobacterium longum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
Bifidobacterium choerinum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Thermus thermophilus
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Trichoderma viride
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Aspergillus sp.
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Bifidobacterium longum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Lissachatina fulica
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Passalora fulva
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Pseudothermotoga thermarum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1 (Rb1->G17->G75). Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively, with the hydrolysis pattern of B (Rd->F2->C-K or Rg3->Rh2->APPD). Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides with 4 different hydrolysis patterns
Flavobacterium johnsoniae
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides
Aspergillus niger
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides
Pseudonocardia sp.
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides
Rhizobium sp.
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides
Microbacterium esteraromaticum
physiological function
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-ii. Ginsenosides Rd, F2, C-K, Re, Rg1, F1 and notoginsenoside R1 are converted to Rg3, Rh2, APPD, Rg2, Rh1, APPT and notoginsenoside R2 without further hydrolysis, respectively, by ginsenosidase type II-ii enzymes through the hydrolysis of the inner glucose at C-20 but not the sugar residues linked to C-3 and C-6 in the PPD- and PPT-type ginsenosides, respectively. Ginsenosidase type II-ii enzymes also hydrolyze the outer sugar, including glucose, arabinopyranose or arabinofuranose linked to C-20 in the PPD-type ginsenosides
Mucilaginibacter sp. QM49
Other publictions for EC 3.2.1.B23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
753334
Shin
Classification of glycosidase ...
Lissachatina fulica, Pseudonocardia sp., Aspergillus niger, Aspergillus sp., Microbacterium esteraromaticum, Bifidobacterium breve, Bifidobacterium longum, Caldicellulosiruptor saccharolyticus, Thermus thermophilus, Rhizobium sp., Trichoderma viride, Panax ginseng, Passalora fulva, Leuconostoc sp., Pseudothermotoga thermarum, Flavobacterium johnsoniae, Bifidobacterium choerinum, Rhodanobacter ginsenosidimutans, Mucilaginibacter sp. QM49, Bifidobacterium longum H-1, Pseudothermotoga thermarum DSM 5069T
Crit. Rev. Biotechnol.
36
1036-1049
2016
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-
-
-
-
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-
-
-
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55
-
21
-
-
-
-
-
-
16
-
60
20
63
16
-
-
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
55
-
-
-
-
-
-
16
-
60
20
16
-
-
-
19
-
-
-
-
37
37
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-
-
679559
Hu
-
Purification and characterizat ...
Lissachatina fulica
Enzyme Microb. Technol.
40
1358-1366
2007
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-
-
-
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-
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2
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1
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1
1
-
-
1
1
-
5
1
-
1
-
2
-
1
1
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
2
-
-
-
1
1
-
1
1
-
5
1
1
-
2
-
1
1
1
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-
-
-
-
-
-