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Information on EC 3.2.1.B23 - ginsenosidase type II
for references in articles please use BRENDA:EC3.2.1.B23preliminary BRENDA-supplied EC number
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EC Tree
3.2.1.B23 ginsenosidase type IISpecify your search results
Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Sequential hydrolysis of the 20-O-beta-D-(1->6)-glucopyranosyl bond (leaving a single glucosyl residue at that position) followed by hydrolysis of the 3-O-beta-D-(1->2)-glucopyranosyl bond and the 3-O-beta-D-glucopyranosyl bond in protopanaxadiol-type ginsenosides
Synonyms
alpha-l-arabinopyranosidase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alpha-L-arabinofuranosidase
alpha-L-arabinopyranosidase
beta-glucosidase
ginsenosidase type II
ginsenosidase type II-i-1
ginsenosidase type II-i-2
ginsenosidase type II-i-4
ginsenosidase type II-ii
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Sequential hydrolysis of the 20-O-beta-D-(1->6)-glucopyranosyl bond (leaving a single glucosyl residue at that position) followed by hydrolysis of the 3-O-beta-D-(1->2)-glucopyranosyl bond and the 3-O-beta-D-glucopyranosyl bond in protopanaxadiol-type ginsenosides
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ginsenoside Mc1 + H2O
ginsenoside F2 + L-arabinofuranose
-
-
-
-
?
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
-
the enzyme hydrolyzes the 20-O-beta-D-(1->6)-glucoside and both glucosidic linkages at 3-C. The enzyme prefers the 20-C, beta-(1->6)-glucosidic bond to the 3-C, beta-(1->2)-glucosidic bond. Ginsenoside Rb1 is firstly converted to ginsenoside Rd, subsequently to ginsenoside F2, and finally to ginsenoside C-K after 24 h of incubation
-
-
?
ginsenoside Rb2 + 2 H2O
ginsenoside C-Y + 2 D-glucose
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the enzyme hydrolyzes both glucosidic linkages at 3-C. Ginsenoside Rb2 is firstly converted to ginsenoside C-O and subsequently to ginsenoside C-Y
-
-
?
ginsenoside Rb2 + H2O
ginsenoside Rg3 + D-glucose + L-arabinopyranose
-
-
-
-
?
ginsenoside Rb3 + H2O
ginsenoside Mx + 2 D-glucose
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the enzyme hydrolyzes both glucosidic linkages at 3-C
-
-
?
ginsenoside Rc + H2O
ginsenoside Mc + 2 D-glucose
-
the enzyme hydrolyzes both glucosidic linkages at 3-C
-
-
?
ginsenoside Mc + H2O
ginsenoside C-K + L-arabinofuranose
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-
-
-
?
ginsenoside Mc + H2O
ginsenoside C-K + L-arabinofuranose
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-
-
-
?
ginsenoside Rc + H2O
ginsenoside Rd + L-arabinofuranose
-
-
-
-
?
ginsenoside Rc + H2O
ginsenoside Rd + L-arabinofuranose
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-
-
-
?
additional information
?
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ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
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-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
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-
?
additional information
?
-
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ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
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-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
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ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
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-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
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ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
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-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
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-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
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-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
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?
additional information
?
-
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the enzyme does not cleave the 20-C-beta-D-(6->1)-arabinopyranoside of ginsenoside Rb2, the 20-C-beta-D-(6->1)-xyloside of ginsenoside Rb3, the 20-O-beta-D-(6->1)-arabinofuranoide of ginsenoside Rc. The enzyme also hydrolyses 4-nitrophenyl-beta-D-glycosides, cellobiose, sophorose, methyl beta-glucopyranoside, hexyl beta-D-glucopyranoside, heptyl beta-D-glucopyranoside, decyl beta-D-glucopyranoside, dodecyl beta-D-glucopyranoside
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?
additional information
?
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ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
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?
additional information
?
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hydrolysis of the C-20 inner Glc
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?
additional information
?
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hydrolysis of the C-20 inner Glc
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?
additional information
?
-
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ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
Pseudothermotoga thermarum DSM 5069T
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ginsenoside Mc1 + H2O
ginsenoside F2 + L-arabinofuranose
-
-
-
-
?
ginsenoside Rb2 + H2O
ginsenoside Rg3 + D-glucose + L-arabinopyranose
-
-
-
-
?
ginsenoside Mc + H2O
ginsenoside C-K + L-arabinofuranose
-
-
-
-
?
ginsenoside Mc + H2O
ginsenoside C-K + L-arabinofuranose
-
-
-
-
?
ginsenoside Rc + H2O
ginsenoside Rd + L-arabinofuranose
-
-
-
-
?
ginsenoside Rc + H2O
ginsenoside Rd + L-arabinofuranose
-
-
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
Pseudothermotoga thermarum DSM 5069T
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-1 enzymes convert ginsenosides Rb1, G17 and G75 to ginsenosides Rd, F2 and CK as final products, respectively, by hydrolyzing only the outer glucose linked to C-20 but not the inner glucose linked to C-20 with the hydrolysis pattern of A-1. Ginsenosidase type II-i-1 catalyze hydrolysis of C-20 outer Glc (hydrolysis pattern A-1)
-
-
?
additional information
?
-
-
ginsenosidase type II enzymes hydrolyze the sugars linked to C-20 in the PPD- and PPT-type ginsenosides, respectively. Type II enzymes are divided into types II-i-1, II-i-2, II-i-3, II-i-4 and II-2. Ginsenosidase type II-i-2 enzymes convert ginsenosides Rb2, C-O and C-Y to ginsenosides Rd, F2 and C-K as final products without further hydrolysis, respectively, through the hydrolysis of the outer arabinopyranose linked to C-20 with the hydrolysis pattern of A-2 (Rb2->C-O->C-Y)
-
-
?
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 6.5
-
pH 4.0: about 40% of maximal activity, pH 6.5: about 50% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
50
90
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
