Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
2 * 110000, SDS-PAGE | Lissachatina fulica |
220000 | - |
native PAGE | Lissachatina fulica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lissachatina fulica | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | might be a glycoprotein | Lissachatina fulica |
Purification (Comment) | Organism |
---|---|
- |
Lissachatina fulica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
viscus | - |
Lissachatina fulica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
162.8 | - |
pH 5.0, 50°C, substrate: 4-nitrophenyl-beta-D-glucopyranoside | Lissachatina fulica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ginsenoside Rb1 + H2O | the enzyme hydrolyzes the 20-O-beta-D-(1->6)-glucoside and both glucosidic linkages at 3-C. The enzyme prefers the 20-C, beta-(1->6)-glucosidic bond to the 3-C, beta-(1->2)-glucosidic bond. Ginsenoside Rb1 is firstly converted to ginsenoside Rd, subsequently to ginsenoside F2, and finally to ginsenoside C-K after 24 h of incubation | Lissachatina fulica | ginsenoside C-K + 3 D-glucose | - |
? | |
ginsenoside Rb2 + 2 H2O | the enzyme hydrolyzes both glucosidic linkages at 3-C. Ginsenoside Rb2 is firstly converted to ginsenoside C-O and subsequently to ginsenoside C-Y | Lissachatina fulica | ginsenoside C-Y + 2 D-glucose | - |
? | |
ginsenoside Rb3 + H2O | the enzyme hydrolyzes both glucosidic linkages at 3-C | Lissachatina fulica | ginsenoside Mx + 2 D-glucose | - |
? | |
ginsenoside Rc + H2O | the enzyme hydrolyzes both glucosidic linkages at 3-C | Lissachatina fulica | ginsenoside Mc + 2 D-glucose | - |
? | |
additional information | the enzyme does not cleave the 20-C-beta-D-(6->1)-arabinopyranoside of ginsenoside Rb2, the 20-C-beta-D-(6->1)-xyloside of ginsenoside Rb3, the 20-O-beta-D-(6->1)-arabinofuranoide of ginsenoside Rc. The enzyme also hydrolyses 4-nitrophenyl-beta-D-glycosides, cellobiose, sophorose, methyl beta-glucopyranoside, hexyl beta-D-glucopyranoside, heptyl beta-D-glucopyranoside, decyl beta-D-glucopyranoside, dodecyl beta-D-glucopyranoside | Lissachatina fulica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 110000, SDS-PAGE | Lissachatina fulica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Lissachatina fulica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 8 h, enzyme retains full activity | Lissachatina fulica |
60 | - |
stable for 30 min, 72% loss of activity after 30 min | Lissachatina fulica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Lissachatina fulica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | pH 4.0: about 40% of maximal activity, pH 6.5: about 50% of maximal activity | Lissachatina fulica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | retains more than 82% of its original activity after 24 h at 30°C | Lissachatina fulica |