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Literature summary for 3.2.1.B1 extracted from

  • Gupta, V.; Trivedi, N.; Kumar, M.; Reddy, CRK.
    Purification and characterization of exo-beta-agarase from an endophytic marine bacterium and its catalytic potential in bioconversion of red algal cell wall polysaccharides into galactans (2013), Biomass Bioenergy, 49, 290-298 .
No PubMed abstract available
Search for more literature for 3.2.1.B1

General Stability

General Stability Organism
the enzyme activity remains stable (more than 80% activity) in the presence of 1-4 M NaCl Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
acetone about 25% residual activity at 10% (v/v) Pseudomonas sp.
Benzene about 65% residual activity at 10% (v/v) Pseudomonas sp.
butanol about 33% residual activity at 10% (v/v) Pseudomonas sp.
chloroform about 20% residual activity at 10% (v/v) Pseudomonas sp.
Co2+ about 30% residual activity at 10 mM Pseudomonas sp.
Cyclohexane about 35% residual activity at 10% (v/v) Pseudomonas sp.
Dichloromethane about 18% residual activity at 10% (v/v) Pseudomonas sp.
DMSO about 45% residual activity at 10% (v/v) Pseudomonas sp.
EDTA about 20% residual activity at 10 mM Pseudomonas sp.
ethanol about 35% residual activity at 10% (v/v) Pseudomonas sp.
heptane about 50% residual activity at 10% (v/v) Pseudomonas sp.
Hg+ about 80% residual activity at 10 mM Pseudomonas sp.
Isopropanol about 40% residual activity at 10% (v/v) Pseudomonas sp.
Li+ about 80% residual activity at 10 mM Pseudomonas sp.
methanol about 36% residual activity at 10% (v/v) Pseudomonas sp.
Mg2+ about 80% residual activity at 10 mM Pseudomonas sp.
Pb2+ about 55% residual activity at 10 mM Pseudomonas sp.
phenylmethylsulfonyl fluoride about 65% residual activity at 10 mM Pseudomonas sp.
SDS about 40% residual activity at 10% (v/v) Pseudomonas sp.
Toluene about 43% residual activity at 10% (v/v) Pseudomonas sp.
Triton X-100 about 59% residual activity at 10% (v/v) Pseudomonas sp.
Tween-20 about 90% residual activity at 10% (v/v) Pseudomonas sp.
Tween-80 about 60% residual activity at 10% (v/v) Pseudomonas sp.
Zn2+ about 30% residual activity at 10 mM Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ about 140% activity at 10 mM Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 native PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agar + H2O Pseudomonas sp.
-
 neoagarobiose + D-galactose
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and DEAE Sephadex A-50 gel filtration Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agar + H2O
-
 Pseudomonas sp. neoagarobiose + D-galactose
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
exo-beta-agarase
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 about 75% activity 25°C, about 90% activity 30°C, 100% activity at 35°C, about 62% activity 40°C, about 58% activity 45°C Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 the enzyme loses 50% of its activity at 35°C after an incubation of 1 h Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 11 the enzyme shows residual activities of about 65%, 75%, 85% and 78% at pH 7.0, 8.0, 10.0 and 11.0, respectively Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
12 13 the enzyme retains about 62% of its activity at pH 12.0 and almost 50% at pH 13.0 after a pre-incubation of 1 h Pseudomonas sp.