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4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
agar + H2O
neoagarobiose + ?
agar + H2O
neoagarobiose + D-galactose
-
-
-
-
?
agar + H2O
neoagarobiose + neoagarotetraose
-
-
-
-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
agar + H2O
neoagarohexaose + neoagarotetraose + neoagarobiose + 3,6-anhydro-alpha-L-galactose
-
-
-
-
?
agar + H2O
neoagarotriose + ?
-
-
-
-
?
agar + H2O
neoagarotriose + neoagarohexaose + neoagaroheptaose
agarose + H2O
neoagarobiose + neoagarohexaose + neoagarotetraose
agarose + H2O
neoagarobiose + neoagarotetraose
agarose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
agarose + H2O
neoagarohexaose + neoagarotetraose
agarose + H2O
neoagarohexaose + neoagarotetraose + D-galactose
agarose + H2O
neoagarohexaose + neoagarotetraose + neoagarobiose
agarose + H2O
neoagarooctaose + neoagarodecaose
agarose + H2O
neoagarooctaose + neoagarodecaose + neoagarododecaose
-
36.9% neoagarooctaose, 32% neoagarodecaose, 16,7% neoagarododecaose and small amounts of neoagarohexaose and neoagarotetraose
-
?
agarose + H2O
neoagarooctaose + neoagarodecaose + neoagarododecaose + neoagarotetradecaose
-
in presence of high amount of enzyme, products are predominantly neoagarooctaose plus some neoagarohexaose and neoagarotetraose
-
?
agarose + H2O
neoagarooctaose + neoagarohexaose
agarose + H2O
neoagarotetraose + neoagarohexaose
agarose + H2O
neoagarotetraose + neoagarohexaose + ?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose
alginate + H2O
?
-
-
-
-
?
carboxymethyl cellulose + H2O
?
Gracilaria lemaneiformis + H2O
neoagarobiose + neoagarotetraose
-
-
-
-
?
Gracilaria lemaneiformis + H2O
neoagarohexaose + neoagarotetraose
neoagarodecaose + H2O
?
-
-
-
?
neoagarodecaose + H2O
neoagarohexaose + neoagarotetraose
enzyme attacks preferentially the sixth linkage and subsequently the eighth linkage from the non-reducing end of neoagarodecaose
-
-
?
neoagarododecaose + H2O
?
-
-
-
?
neoagarohexaose + H2O
neoagarotetraose + neoagarobiose
neoagarotetradecaose + H2O
?
-
-
-
?
neoagarotetraose + H2O
neoagarobiose
porphyran + H2O
neoagarooctaose
-
main product, plus neoagarotetraose and neoagarohexaose
-
?
starch + H2O
?
1.8% activity compared to agarose
-
-
?
additional information
?
-
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
negligible activity
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
negligible activity
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
strong hydrolytic activity
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
strong hydrolytic activity
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
-
-
-
?
agar + H2O
?
-
-
-
-
?
agar + H2O
neoagarobiose + ?
-
-
-
-
?
agar + H2O
neoagarobiose + ?
-
-
-
-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
100% activity towards biological agar. The relative activities to agarose for electrophoresis, edible agar and low-melting agar are 75.9, 70.9 and 49.3%, respectively
neoagarobiose is the main product
-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
100% activity towards biological agar. The relative activities to agarose for electrophoresis, edible agar and low-melting agar are 75.9, 70.9 and 49.3%, respectively
neoagarobiose is the main product
-
?
agar + H2O
neoagarotriose + neoagarohexaose + neoagaroheptaose
-
-
-
-
?
agar + H2O
neoagarotriose + neoagarohexaose + neoagaroheptaose
-
-
-
-
?
agarose + H2O
?
-
-
the enzyme mainly produces trisaccharide, as well as a small amount of disaccharides, tetrose, pentasaccharide, and hexose
-
?
agarose + H2O
?
-
-
-
-
?
agarose + H2O
?
-
beta-agarase hydrolyzes beta-1,4-linkages of agarose
-
-
?
agarose + H2O
neoagarobiose + neoagarohexaose + neoagarotetraose
-
-
neoagarobiose is the main product
-
?
agarose + H2O
neoagarobiose + neoagarohexaose + neoagarotetraose
-
-
neoagarobiose is the main product
-
?
agarose + H2O
neoagarobiose + neoagarotetraose
-
-
products of isoform agarase-b
-
?
agarose + H2O
neoagarobiose + neoagarotetraose
-
-
products of isoform agarase-b
-
?
agarose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
-
-
products of isoform agarase-a
-
?
agarose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
-
-
products of isoform agarase-a
-
?
agarose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
-
the enzyme cleaves the beta-1,4-linkage in agarose to produce neoagarooligosaccharides mainly composed of neoagarobiose, neoagarotetraose, and neoagarohexaose
main products
-
?
agarose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
-
the enzyme cleaves the beta-1,4-linkage in agarose to produce neoagarooligosaccharides mainly composed of neoagarobiose, neoagarotetraose, and neoagarohexaose
main products
-
?
agarose + H2O
neoagarohexaose + neoagarotetraose
-
-
-
-
?
agarose + H2O
neoagarohexaose + neoagarotetraose
-
-
-
-
?
agarose + H2O
neoagarohexaose + neoagarotetraose + D-galactose
100% activity
-
-
?
agarose + H2O
neoagarohexaose + neoagarotetraose + D-galactose
-
-
-
-
?
agarose + H2O
neoagarohexaose + neoagarotetraose + neoagarobiose
-
-
-
-
?
agarose + H2O
neoagarohexaose + neoagarotetraose + neoagarobiose
-
-
-
-
?
agarose + H2O
neoagarooctaose + neoagarodecaose
-
major end products
-
?
agarose + H2O
neoagarooctaose + neoagarodecaose
-
main end products, plus small amounts of neoagarotetraose and neoagarohexaose
-
?
agarose + H2O
neoagarooctaose + neoagarohexaose
-
-
main hydrolysates of agarose by agarase AG-b are neoagarooctaose (30%) and neoagarohexaose (67%)
-
?
agarose + H2O
neoagarooctaose + neoagarohexaose
-
-
main hydrolysates of agarose by agarase AG-b are neoagarooctaose (30%) and neoagarohexaose (67%)
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose
the recombinant enzyme hydrolyzes the beta-1,4-glycosidic linkages of agarose
main products
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose
-
the purified enzyme is an endo-type beta-agarase able to hydrolyze the beta-1,4 glycosidic linkages of agarose, releasing neoagarotetraose and neoagarohexaose
major end products
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose
-
-
-
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + ?
-
main products
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + ?
-
main products
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose
-
-
neoagarotetraose and neoagarohexaose are main products, while neoagarooctaose is a minor product
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose
-
-
neoagarotetraose and neoagarohexaose are main products, while neoagarooctaose is a minor product
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose
-
neoagarotetraose and neoagarohexaose are the main products
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose
-
neoagarotetraose and neoagarohexaose are the main products
-
?
carboxymethyl cellulose + H2O
?
1.4% activity compared to agarose
-
-
?
carboxymethyl cellulose + H2O
?
-
-
-
-
?
carrageenan + H2O
?
-
-
-
-
?
carrageenan + H2O
?
-
-
-
-
?
carrageenan + H2O
?
-
-
-
-
?
Gracilaria lemaneiformis + H2O
neoagarohexaose + neoagarotetraose
-
-
-
-
?
Gracilaria lemaneiformis + H2O
neoagarohexaose + neoagarotetraose
-
-
-
-
?
neoagarohexaitol + H2O
?
-
the enzyme the cleaves the beta-1,4 linkage near a sugar alcohol in neoagarohexaitol
-
-
?
neoagarohexaitol + H2O
?
-
the enzyme the cleaves the beta-1,4 linkage near a sugar alcohol in neoagarohexaitol
-
-
?
neoagarohexaose + H2O
neoagarotetraose + neoagarobiose
-
-
-
-
?
neoagarohexaose + H2O
neoagarotetraose + neoagarobiose
-
-
-
-
?
neoagarotetraose + H2O
neoagarobiose
-
-
-
?
neoagarotetraose + H2O
neoagarobiose
-
-
-
?
seaweed + H2O
?
-
-
-
-
?
seaweed + H2O
?
-
-
-
-
?
additional information
?
-
the enzyme cannot cleave neoagarohexaose and shorter oligomers
-
-
?
additional information
?
-
the enzyme does not hydrolyze 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
additional information
?
-
the enzyme does not hydrolyze 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
additional information
?
-
-
the purified enzyme fails to hydrolyze carboxymethylcellulose, dextran, soluble starch, pectin, and polygalacturonic acid
-
-
?
additional information
?
-
-
the purified enzyme fails to hydrolyze carboxymethylcellulose, dextran, soluble starch, pectin, and polygalacturonic acid
-
-
?
additional information
?
-
enzyme has a large substrate binding cleft that accomodates 12 sugar units, with 8 sugar units toward the reducing end spanning subsites +1 to +8 and 4 sugar units toward the non-reducing end spanning subsites -4 to -1. No substrates are kappa-carrageenan, iota-carrageenan, lambda-carrageenan, alginate, and chitosan and oligosaccharides with a degree of polymerization below 10
-
-
?
additional information
?
-
-
enzyme has a large substrate binding cleft that accomodates 12 sugar units, with 8 sugar units toward the reducing end spanning subsites +1 to +8 and 4 sugar units toward the non-reducing end spanning subsites -4 to -1. No substrates are kappa-carrageenan, iota-carrageenan, lambda-carrageenan, alginate, and chitosan and oligosaccharides with a degree of polymerization below 10
-
-
?
additional information
?
-
-
no activity with 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
additional information
?
-
does not hydrolyze lambda-carrageenan, kappa-carrageenan and, iota-carrageenan
-
-
?
additional information
?
-
does not hydrolyze lambda-carrageenan, kappa-carrageenan and, iota-carrageenan
-
-
?
additional information
?
-
enzyme does not hydrolyze neoagarooctaose or smaller neoagarooligosaccharides. No substrate: kappa-carrageenan
-
-
?
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agar + H2O
neoagarobiose + ?
-
-
-
-
?
agar + H2O
neoagarobiose + D-galactose
-
-
-
-
?
agar + H2O
neoagarobiose + neoagarotetraose
-
-
-
-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
agar + H2O
neoagarotriose + ?
-
-
-
-
?
agarose + H2O
neoagarobiose + neoagarohexaose + neoagarotetraose
agarose + H2O
neoagarobiose + neoagarotetraose
agarose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
agarose + H2O
neoagarohexaose + neoagarotetraose + D-galactose
agarose + H2O
neoagarohexaose + neoagarotetraose + neoagarobiose
agarose + H2O
neoagarotetraose + neoagarohexaose + ?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose
carboxymethyl cellulose + H2O
?
1.4% activity compared to agarose
-
-
?
Gracilaria lemaneiformis + H2O
neoagarobiose + neoagarotetraose
-
-
-
-
?
neoagarotetraose + H2O
neoagarobiose
starch + H2O
?
1.8% activity compared to agarose
-
-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
100% activity towards biological agar. The relative activities to agarose for electrophoresis, edible agar and low-melting agar are 75.9, 70.9 and 49.3%, respectively
neoagarobiose is the main product
-
?
agar + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
100% activity towards biological agar. The relative activities to agarose for electrophoresis, edible agar and low-melting agar are 75.9, 70.9 and 49.3%, respectively
neoagarobiose is the main product
-
?
agarose + H2O
?
-
-
the enzyme mainly produces trisaccharide, as well as a small amount of disaccharides, tetrose, pentasaccharide, and hexose
-
?
agarose + H2O
?
-
-
-
-
?
agarose + H2O
neoagarobiose + neoagarohexaose + neoagarotetraose
-
-
neoagarobiose is the main product
-
?
agarose + H2O
neoagarobiose + neoagarohexaose + neoagarotetraose
-
-
neoagarobiose is the main product
-
?
agarose + H2O
neoagarobiose + neoagarotetraose
-
-
products of isoform agarase-b
-
?
agarose + H2O
neoagarobiose + neoagarotetraose
-
-
products of isoform agarase-b
-
?
agarose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
-
-
products of isoform agarase-a
-
?
agarose + H2O
neoagarobiose + neoagarotetraose + neoagarohexaose
-
-
products of isoform agarase-a
-
?
agarose + H2O
neoagarohexaose + neoagarotetraose + D-galactose
100% activity
-
-
?
agarose + H2O
neoagarohexaose + neoagarotetraose + D-galactose
-
-
-
-
?
agarose + H2O
neoagarohexaose + neoagarotetraose + neoagarobiose
-
-
-
-
?
agarose + H2O
neoagarohexaose + neoagarotetraose + neoagarobiose
-
-
-
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + ?
-
main products
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + ?
-
main products
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose
-
-
neoagarotetraose and neoagarohexaose are main products, while neoagarooctaose is a minor product
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose
-
-
neoagarotetraose and neoagarohexaose are main products, while neoagarooctaose is a minor product
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose
-
neoagarotetraose and neoagarohexaose are the main products
-
?
agarose + H2O
neoagarotetraose + neoagarohexaose + neoagarooctaose + neoagarodecaose
-
neoagarotetraose and neoagarohexaose are the main products
-
?
neoagarotetraose + H2O
neoagarobiose
-
-
-
?
neoagarotetraose + H2O
neoagarobiose
-
-
-
?
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5,5'-dithiobis-(2-nitrobenzoic acid)
69% residual activity at 1 mM
butan-1-ol
-
about 30% residual activity at 10% (v/v)
butanol
-
about 33% residual activity at 10% (v/v)
Cyclohexane
-
about 35% residual activity at 10% (v/v)
ethenol
-
about 30% residual activity at 10% (v/v)
ethyl acetate
-
12.1% residual activity at 5% (v/v)
heptane
-
about 50% residual activity at 10% (v/v)
hexane
-
76.9% residual activity at 5% (v/v)
Hg+
-
about 80% residual activity at 10 mM
Isopropanol
-
about 40% residual activity at 10% (v/v)
K+
-
97.26% residual activity at 5 mM
Na+
-
60% residual activity at 1 mM, 72% residual activity at 10 mM, 91% residual activity at 100 mM, 83% residual activity at 1 M, complete inhibition at 3 M
Ni2+
1% residual activity at 10 mM
phenylmethylsulfonyl fluoride
Tween-20
-
about 90% residual activity at 10% (v/v)
acetone
-
86.9% residual activity at 5% (v/v)
acetone
-
about 30% residual activity at 10% (v/v)
acetone
-
about 25% residual activity at 10% (v/v)
Ag+
-
15% residual activity at 1 mM
Ag+
-
15% residual activity at 1 mM
Ag+
-
5 mM, complete inhibition of agarase AG-b
Al3+
strong inhibition
Al3+
-
56.3% residual activity at 10 mM
Ba2+
-
50% residual activity at 2 mM
Ba2+
-
85% residual activity at 1 mM
Ba2+
-
16% residual activity at 1 mM
Ba2+
-
16% residual activity at 1 mM
Benzene
-
about 70% residual activity at 10% (v/v)
Benzene
-
about 65% residual activity at 10% (v/v)
Ca2+
complete inhibition at 5 mM
Ca2+
-
89% residual activity at 2 mM
Ca2+
-
96.78% residual activity at 5 mM
Ca2+
-
about 90% residual activity at 2 mM
Cd2+
-
about 85% residual activity at 2 mM
Cd2+
-
50.9% residual activity at 10 mM
chloroform
-
about 25% residual activity at 10% (v/v)
chloroform
-
about 20% residual activity at 10% (v/v)
Co2+
-
at 1 mM, isoforms agarase-a and agarase-b show 81.31% and 91.81% residual activity, respectively
Co2+
-
complete inhibition at 2mM
Co2+
-
complete inhibition at 1 mM
Co2+
-
about 30% residual activity at 1 mM
Co2+
-
about 30% residual activity at 10 mM
Co2+
-
87.1% residual activity at 10 mM
Cu2+
-
at 1 mM, isoforms agarase-a and agarase-b show 72.2% and 64.54% residual activity, respectively
Cu2+
69% residual activity at 1 mM
Cu2+
-
complete inhibition at 2mM
Cu2+
34% residual activity at 1 mM
Cu2+
-
complete inhibition at 1 mM
Cu2+
-
6.2% residual activity at 5 mM
Cu2+
-
about 40% residual activity at 2 mM
Cu2+
-
15.8% residual activity at 10 mM
Dichloromethane
-
about 20% residual activity at 10% (v/v)
Dichloromethane
-
about 18% residual activity at 10% (v/v)
DMSO
-
79.3% residual activity at 5% (v/v)
DMSO
-
about 40% residual activity at 10% (v/v)
DMSO
-
about 45% residual activity at 10% (v/v)
EDTA
-
EDTA reduces the agarase activity by 58% at 5 mM
EDTA
-
at 2 mM, isoforms agarase-a shows 71.32% residual activity
EDTA
69% residual activity at 10 mM
EDTA
-
complete inhibition at 2mM
EDTA
81% residual activity at 1 mM
EDTA
-
46% residual activity at 1 mM
EDTA
-
92% residual activity at 2 mM
EDTA
-
86.47% residual activity at 5 mM
EDTA
-
about 22% residual activity at 2 mM
EDTA
-
about 20% residual activity at 1 mM
EDTA
-
about 20% residual activity at 10 mM
EDTA
-
77% residual activity at 1 mM
EDTA
-
77% residual activity at 1 mM
EDTA
-
68.1% residual activity at 10 mM
EDTA
-
5 mM, 15% inhibition of agarase AG-b
ethanol
-
76.9% residual activity at 5% (v/v)
ethanol
-
about 35% residual activity at 10% (v/v)
Fe2+
-
at 1 mM, isoform agarase-b shows 86.36% residual activity
Fe2+
-
complete inhibition at 2mM
Fe2+
-
complete inhibition at 1 mM
Fe2+
-
77% residual activity at 2 mM
Fe2+
-
about 68% residual activity at 2 mM
Fe2+
-
53% residual activity at 1 mM
Fe2+
-
53% residual activity at 1 mM
Fe2+
-
79.6% residual activity at 10 mM
Fe3+
14% residual activity at 1 mM
Fe3+
-
34.65% residual activity at 5 mM
Fe3+
-
15% residual activity at 1 mM
Fe3+
-
15% residual activity at 1 mM
Fe3+
-
4.4% residual activity at 10 mM
Fe3+
-
1 mM, 65% inhibition of agarase AG-b
Hg2+
-
complete inhibition at 1 mM
Hg2+
-
about 80% residual activity at 1 mM
Hg2+
-
5 mM, complete inhibition of agarase AG-b
Li+
-
92% residual activity at 5 mM
Li+
-
about 80% residual activity at 1 mM
Li+
-
about 80% residual activity at 10 mM
methanol
-
72.6% residual activity at 5% (v/v)
methanol
-
about 35% residual activity at 10% (v/v)
methanol
-
about 36% residual activity at 10% (v/v)
Mg2+
65% residual activity at 1 mM
Mg2+
78% residual activity at 1 and 5 mM
Mg2+
88% residual activity at 1 mM
Mg2+
-
76.92% residual activity at 5 mM
Mg2+
-
about 80% residual activity at 1 mM
Mg2+
-
about 80% residual activity at 10 mM
Mg2+
-
46% residual activity at 1 mM
Mg2+
-
46% residual activity at 1 mM
Mn2+
-
at 1 mM, isoforms agarase-a and agarase-b show 86.14% and 62.72% residual activity, respectively
Mn2+
complete inhibition at 5 mM
Mn2+
-
complete inhibition at 2mM
Mn2+
28% residual activity at 1 mM
Mn2+
-
70% residual activity at 2 mM
Mn2+
-
28.6% residual activity at 10 mM
Pb2+
-
about 50% residual activity at 1 mM
Pb2+
-
about 55% residual activity at 10 mM
Pb2+
-
7% residual activity at 1 mM
Pb2+
-
7% residual activity at 1 mM
phenylmethylsulfonyl fluoride
-
about 60% residual activity at 1 mM
phenylmethylsulfonyl fluoride
-
about 65% residual activity at 10 mM
SDS
-
about 83% residual activity at 5 mM
SDS
53% residual activity at 10 mM
SDS
-
91% residual activity at 2 mM
SDS
-
about 60% residual activity at 10% (w/v)
SDS
-
about 40% residual activity at 10% (v/v)
SDS
-
54% residual activity at 1 mM
SDS
-
54% residual activity at 1 mM
SDS
-
72.8% residual activity at 1% (w/v)
SDS
-
5 mM, 16% inhibition of agarase AG-b
Sn2+
-
30% residual activity at 1 mM
Sn2+
-
30% residual activity at 1 mM
Toluene
-
about 40% residual activity at 10% (v/v)
Toluene
-
about 43% residual activity at 10% (v/v)
Triton X-100
-
58% residual activity at 10% (v/v)
Triton X-100
-
about 59% residual activity at 10% (v/v)
Tween 20
-
89.2% residual activity at 10% (v/v)
Tween 20
-
95.7% residual activity at 1% (v/v)
Tween 80
-
60.2% residual activity at 10% (v/v)
Tween 80
-
97.5% residual activity at 1% (v/v)
Tween-80
-
89.72% residual activity at 0.5% (v/v)
Tween-80
-
about 60% residual activity at 10% (v/v)
Urea
-
about 80% residual activity at 5 mM
Urea
-
84.8% residual activity at 5 M
Zn2+
strong inhibition
Zn2+
% residual activity at 47 mM
Zn2+
-
complete inhibition at 2mM
Zn2+
-
complete inhibition at 1 mM
Zn2+
-
81% residual activity at 2 mM
Zn2+
-
14.97% residual activity at 5 mM
Zn2+
-
about 30% residual activity at 1 mM
Zn2+
-
about 30% residual activity at 10 mM
Zn2+
-
23% residual activity at 1 mM
Zn2+
-
23% residual activity at 1 mM
Zn2+
-
17.5% residual activity at 10 mM
additional information
-
isoforms agarase-a and agarase-b are not inhibited by urea and SDS
-
additional information
not influenced by EDTA, 2-mercaptoethanol, dithiothreitol, and urea
-
additional information
no significant activation or inhibition is observed urea
-
additional information
not inhibited by EDTA
-
additional information
-
not inhibited by EDTA
-
additional information
urea and SDS do not have obvious effects on the activity
-
additional information
-
urea and SDS do not have obvious effects on the activity
-
additional information
-
not inhibited by urea and SDS
-
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10 - 50
-
up to 80% thermostability of the enzyme is retained at 10°C and 30 °C for 180 min. When the temperature is increased to 50°C for 30 min, the enzyme relative activity is decreased to 10-20%. Nevertheless, the enzyme is fairly stable (almost 70% relative activity) at 40°C for 30 min
20 - 50
-
isoform garase-a is stable at 30°C, while agarase-b is stable at 50°C. Agarase-a shows residual activity remaining above 70% after 60 min at 2030°C. After incubation at 40°C for 60 min, the remaining activity decreases to 28%. Agarase-b shows more than 75% of the maximal activity at 50°C
20 - 60
-
the enzyme is relatively stable at 20°C and 30°C after incubation for 1 h. Incubation at higher temperatures results in enzyme activity loss. The enzyme retains 64% and 46% of residual activities after incubation at 40°C and 50°C for 1 h, individually. It has no activity at 60°C for 30 min
20 - 80
100% activity between 20 and40°C, about 50% activity at 50°C, about 10% activity between 60-80°C (after incubation for 1 h)
30 - 40
the residual activity is above 79% at 30 and 35°C. The half-life is 56 min at 40°C
30 - 50
-
the enzyme is very stable after 30 min incubation at 30-50°C. At 60°C, the enzyme retains 40% activity
35 - 50
the enzyme is thermostable at temperatures from 35-50°C with more than 80% activity remaining after 30 min incubation
37 - 45
-
the enzyme retains 90.12% activity at 37°C after 2 h, more than 55% activity at 40 and 42°C after 1 h, and 36.16% activity at 45°C after 1 h
40
-
half-life of mutant S2 is 350 min, which was 18.4fold longer than that of wild-type AgaB
40 - 45
the enzyme is stable up to 40°C (100%), and retains more than 70% of its initial activity at 45°C after heat treatment for 30 min. The enzyme stability dramatically declines at 50°C to 19%
40 - 80
-
91.9% and 84.5% of activity are retained after pre-incubation at 40°C and 50°C for 2 h, respectively. 62.5% of the enzyme activity is lost after pre-incubation at 60°C for 2 h, and the enzyme activity is completely lost after pre-incubation at 70°C and 80°C
45
-
30 min, about 10% loss of activity, agarase AG-b
49
-
melting temperature of wild-type enzyme is 49.2°C
50
-
the enzyme is stable up to 1 h at 50°C
50 - 70
-
the agarase retains 62% of its activity after incubating at 50°C for 30 min. Further, 45% of the agarase activity is still retained at 60°C. The agarase activity is completely abolished at 70°C
50 - 80
the enzyme retains more than 80% agarolytic activity after being kept in 50°C for 1 h, 13% residual activity after incubation in 80°C for 1 h, and 12% residual activity if boiled for 5 min
54
-
melting temperature of mutant enzyme S2 is 53.8°C
55
-
30 min, about 20% loss of activity, agarase AG-b
65
-
30 min, about 50% loss of activity, agarase AG-b
70
-
30 min, about 90% loss of activity, agarase AG-b
80
-
more than 50% activity is retained at 80°C for 15 min
10 - 40
-
the activity of agarase is stable at a low temperature and retains more than 90% of its activity up to a temperature of 40°C, after 30 min incubation
10 - 40
-
more than 90% activity is retained after 30 min at 10-40°C
35
stable up to
35
-
wild-type AgaB has low thermostability when temperature is above 35°C
35
-
the enzyme loses 50% of its activity at 35°C after an incubation of 1 h
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74-79
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-
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-
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-
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