Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 15% residual activity at 1 mM | Rhodococcus sp. Q5 | |
Ba2+ | 16% residual activity at 1 mM | Rhodococcus sp. Q5 | |
EDTA | 77% residual activity at 1 mM | Rhodococcus sp. Q5 | |
Fe2+ | 53% residual activity at 1 mM | Rhodococcus sp. Q5 | |
Fe3+ | 15% residual activity at 1 mM | Rhodococcus sp. Q5 | |
Mg2+ | 46% residual activity at 1 mM | Rhodococcus sp. Q5 | |
Pb2+ | 7% residual activity at 1 mM | Rhodococcus sp. Q5 | |
SDS | 54% residual activity at 1 mM | Rhodococcus sp. Q5 | |
Sn2+ | 30% residual activity at 1 mM | Rhodococcus sp. Q5 | |
Zn2+ | 23% residual activity at 1 mM | Rhodococcus sp. Q5 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Rhodococcus sp. Q5 | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no significant activation or inhibition of the enzyme is observed in the presence of Na+, K+ or Ca2+ | Rhodococcus sp. Q5 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
agar + H2O | Rhodococcus sp. Q5 | - |
neoagarotriose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. Q5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification by ammonium sulfate precipitation | Rhodococcus sp. Q5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agar + H2O | - |
Rhodococcus sp. Q5 | neoagarotriose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, SDS-PAGE | Rhodococcus sp. Q5 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Rhodococcus sp. Q5 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | about 45% activity 20°C, about 70% activity 30°C, 100% activity 40°C, about 45% activity 50°C | Rhodococcus sp. Q5 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | more than 90% activity is retained after 30 min at 10-40°C | Rhodococcus sp. Q5 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Rhodococcus sp. Q5 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | more than 50% activity between pH 5.0 and 7.0 | Rhodococcus sp. Q5 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | the enzyme is stable for 30 min in the pH range from pH 5.0 to 8.0, and more than 87% of the residual activity is retained | Rhodococcus sp. Q5 |