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Literature summary for 3.2.1.B1 extracted from

  • Zhang, L.; Lu, X.; Han, F.; Ma, C.; Yu, W.
    Purification and refolding of a novel beta-agarase from inclusion body of E. coli (2007), J. Ocean Univ. China, 6, 80-84.
No PubMed abstract available
Search for more literature for 3.2.1.B1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with C-terminal His-tag Pseudoalteromonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Pseudoalteromonas sp.
EDTA
-
 Pseudoalteromonas sp.
Mn2+
-
 Pseudoalteromonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ slight stimulation Pseudoalteromonas sp.
Ca2+ slight stimulation Pseudoalteromonas sp.

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. A1A3Y9 isoform AgaB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purification from inclusion bodies Pseudoalteromonas sp.

Renatured (Commentary)

Renatured (Comment) Organism
refolding of enzyme from aggregates in inclusion bodies is optimal in buffer containing 20 mM phosphate, 1 M urea, 500 mM L-arginine, and 10% glycerol, pH 7.0, at 4°C Pseudoalteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Pseudoalteromonas sp. neoagarooctaose + neoagarodecaose major end products ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudoalteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pseudoalteromonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.7 10.6
-
 Pseudoalteromonas sp.