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Literature summary for 3.2.1.96 extracted from

  • Li, S.C.; Asakawa, M.; Hirabayashi, Y.; Li, Y.T.
    Isolation of two endo-beta-N-acetylglucosaminidases from fig latex (1981), Biochim. Biophys. Acta, 660, 278-283.
    View publication on PubMed
Search for more literature for 3.2.1.96

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17500
-
 enzyme type F-II, gel filtration Ficus carica
52000
-
 enzyme type F-I, gel filtration Ficus carica

Organism

Organism UniProt Comment Textmining
Ficus carica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
endo-beta-N-acetylglucosaminidase F-I and F-II Ficus carica

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
 Ficus carica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Man)3(GlcNAc)2Asn-Ac + H2O di-N-acetylchitobiosyl linkage is hydrolyzed faster than the linkage in the glycopeptides (Man)5(GlcNAc)3Asn and (Man)6(GlcNAc)2Asn by enzyme form F-I. No activity with the enzyme form F-II Ficus carica (Man)3GlcNAc + GlcNAc-Asn-Ac
-
 ?
(Man)5(GlcNAc)2-N-acetyl-Asn + H2O
-
 Ficus carica (Man)5GlcNAc + GlcNAc-N-acetyl-Asn
-
 ?
(Man)5(GlcNAc)2Asn-Ac + H2O di-N-acetylchitobiosyl linkage is hydrolyzed faster than the linkage in the glycopeptides (Man)5(GlcNAc)3Asn and (Man)6(GlcNAc)2Asn by enzyme form F-I Ficus carica (Man)5GlcNAc + GlcNAc-Asn-Ac
-
 ?
(Man)6(GlcNAc)2Asn-Ac + H2O di-N-acetylchitobiosyl linkage is hydrolyzed faster than the linkage in the glycopeptides (Man)5(GlcNAc)3Asn and (Man)6(GlcNAc)2Asn by enzyme form F-I Ficus carica (Man)6GlcNAc + GlcNAc-Asn-Ac
-
 ?
additional information enzyme type F-I hydrolyzes the tri-mannosyl derivatives di-N-acetylglycosaminylasparagine faster than the penta-mannosyl compounds and the hexamannosyl compounds. Enzyme type F-II hydrolyzes the penta-mannosyl derivatives and hexa-mannosyl derivatives, but not the tri-mannosyl compounds Ficus carica ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 hydrolysis of (Man)5(GlcNAc)2Asn-acetyl, enzyme type F-II Ficus carica
5.9
-
 hydrolysis of (Man)5(GlcNAc)2Asn-acetyl, enzyme type F-I Ficus carica

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.2 8 room temperature, 24 h, enzyme type F-II retains 80-100% of its activity Ficus carica
5 8 room temperature, 24 h, enzyme type F-I is stable, unstable below pH 5 Ficus carica