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Information on EC 3.2.1.96 - mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase
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3.2.1.96 mannosyl-glycoprotein endo-beta-N-acetylglucosaminidaseIUBMB Comments
A group of related enzymes.
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Word Map
- 3.2.1.96
- oligosaccharide
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n-linked
- lysosomal
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high-mannose
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deglycosylation
- n-acetylglucosamine
- glycopeptides
- beta-glucuronidase
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analbuminemic
- n-glycans
- alpha-mannosidase
- glycosidases
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concanavalin
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asparagine-linked
- tunicamycin
- mastitis
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complex-type
- neuraminidase
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pngase
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exoglycosidases
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transglycosylation
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sialylated
- autolysins
- phytolacca
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oxazoline
- swainsonine
- mucor
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3.2.1.30
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mannose-type
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h-sensitive
- alpha-fucosidase
-
udder
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biantennary
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high-mannose-type
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3hmannose
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hybrid-type
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intramammary
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h-resistant
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chitinolytic
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lipid-linked
- 1-deoxymannojirimycin
- beta-n-acetyl-d-glucosaminidase
- glcnac2
- man8glcnac2
- synthesis
- nutrition
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tetraantennary
- meningosepticum
- chitobiose
-
oligomannosidic
- analysis
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holins
- medicine
- glc3man9glcnac2
- food industry
The enzyme appears in viruses and cellular organisms
Synonyms
nagase, beta-n-acetylglucosaminidase, endo-beta-n-acetylglucosaminidase h, endo h, endo-beta-n-acetylglucosaminidase, murein hydrolase, endod, engase, endo-beta-n-acetylglucosaminidase f, endo-m, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylglucosaminidase, endo-beta
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beta-N-acetylglucosaminidase
CBM32
family 32 carbohydrate-binding module of endo-beta-1,4-N-acetylglucosamidase EndoD
di-N-acetylchitobiosyl beta-N-acetylglucosaminidase
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DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F1
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DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F2
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DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase F3
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Endo D
Endo H
Endo-A
endo-beta-(1->4)-N-acetylglucosaminidase
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endo-beta-acetylglucosaminidase
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endo-beta-GlcNAc-ase
endo-beta-N-acetylglucosaminidase
endo-beta-N-acetylglucosaminidase H
Endo-BH
Endo-CC1
Endo-CC2
endo-M
endo-N-acetyl-beta-D-glucosaminidase
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endo-N-acetyl-beta-glucosaminidase
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EndoBI-1
EndoBI-2
endoE
endoglycosidase F1
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endoglycosidase F2
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endoglycosidase F3
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endoglycosidase S
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endohexosaminidase
ENGase
ENGase St
mannosyl-glycoprotein 1,4-N-acetamidodeoxy-beta-D-glycohydrolase
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Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase
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Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F1
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Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F2
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Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase F3
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murein hydrolase
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additional information
endo-beta-N-acetylglucosaminidase
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Endo-BH
Halalkalibacterium halodurans C-125
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additional information
the enzyme belongs to the glycoside hydrolase family 85, GH85
additional information
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the enzyme belongs to the glycoside hydrolase family 85, GH85
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(Man)3(GlcNAc)2Asn + H2O = (Man)3GlcNAc + GlcNAc-Asn
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(Man)3(GlcNAc)2Asn + H2O = (Man)3GlcNAc + GlcNAc-Asn
active site structure, catalytic mechanism in which residue E173 acts as the catalytic acid/base for reaction via an oxazoline intermediate, overview
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
glycopeptide-D-mannosyl-N4-(N-acetyl-D-glucosaminyl)2-asparagine 1,4-N-acetyl-beta-glucosaminohydrolase
A group of related enzymes.
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CAS REGISTRY NUMBER
COMMENTARY
37278-88-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2S)-3-(benzyloxy)butane-1,2,4-triol + sialylglycopeptide
?
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transglycosylation
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-
?
(GlcNAc)2(Man)6(GlcNAc)2 + H2O
GlcNAc + (GlcNAc)1(Man)6(GlcNAc)2
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?
(Man)3(GlcNAc)2-N-Asn-dansyl + H2O
?
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endo-beta-N-acetylglucosaminidase PII, no activity with endo-beta-N-acetylglucosaminidase PII
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?
(Man)3(GlcNAc)2-N-dansyl-Asn + H2O
GlcNAc-N-dansyl-Asn + (Man)3-GlcNAc
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-
-
?
(Man)3(GlcNAc)2Asn + H2O
(Man)3GlcNAc + GlcNAc-Asn
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di-N-acetylchitobiosyl linkage is hydrolyzed faster than the linkage in the glycopeptides (Man)5(GlcNAc)3Asn and (Man)6(GlcNAc)2Asn by enzyme form F-I. No activity with the enzyme form F-II
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?
(Man)3(GlcNAc)2Asn-Ac + H2O
(Man)3GlcNAc + GlcNAc-Asn-Ac
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di-N-acetylchitobiosyl linkage is hydrolyzed faster than the linkage in the glycopeptides (Man)5(GlcNAc)3Asn and (Man)6(GlcNAc)2Asn by enzyme form F-I. No activity with the enzyme form F-II
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?
(Man)5(GlcNAc)2-N-dansyl-Asn + H2O
GlcNAc-N-dansyl-Asn + (Man)5-GlcNAc
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?
(Man)5(GlcNAc)2-pyridylamino + H2O
(Man)5(GlcNAc) + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
(Man)5(GlcNAc)2Asn-Ac + H2O
(Man)5GlcNAc + GlcNAc-Asn-Ac
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di-N-acetylchitobiosyl linkage is hydrolyzed faster than the linkage in the glycopeptides (Man)5(GlcNAc)3Asn and (Man)6(GlcNAc)2Asn by enzyme form F-I
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-
?
(Man)6(GlcNAc)2-Asn + D-glucose
(Man)6GlcNAcGlc + ?
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?
(Man)6(GlcNAc)2Asn-Ac + H2O
(Man)6GlcNAc + GlcNAc-Asn-Ac
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di-N-acetylchitobiosyl linkage is hydrolyzed faster than the linkage in the glycopeptides (Man)5(GlcNAc)3Asn and (Man)6(GlcNAc)2Asn by enzyme form F-I
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?
1,3-di-O-benzyl-D-erythritol + sialylglycopeptide
?
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transglycosylation
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?
2-methyl-[alpha-D-mannopyranosyl-(1,3)-beta-D-mannopyranosyl-(1,4)-alpha-D-glucopyrano]-[2,1-d]-oxazoline + N4-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-1-methyl-N2-(benzyloxycarbonyl)-L-asparagine
(N4-(alpha-D-mannopyranosyl)-(1,3)-beta-D-mannopyranosyl-(1,4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-1-methyl-N2-benzyloxycarbonyl)-L-asparagine + ?
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yield 81%
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?
4-methylumbelliferyl N,N',N''-triacetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N,N',N''-triacetyl-beta-D-glucosaminide
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?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferol + alpha-D-mannopyranose
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?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranose
4-methylumbelliferyl-beta-D-mannopyranoside + H2O
4-methylumbelliferone + beta-D-mannopyranose
4-methylumbelliferyl-beta-D-N-acetylglucosaminide + H2O
4-methylumbelliferone + beta-D-N-acetylglucosamine
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?
4-methylumbelliferyl-beta-mannopyranoside + H2O
4-methylumbelliferol + beta-D-mannopyranose
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?
4-nitrophenyl beta-N-acetyl-D-glucosamine + H2O
4-nitrophenol + N-acetyl-D-glucosamine
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?
4-nitrophenyl-GlcNAc + Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-ManGlcNAc2]-Lys-Thr
?
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?
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide + 4-nitrophenyl-beta-D-glucopyranoside
?
transglycosylation activity
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?
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide + H2O
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked N-acetylglucosamine (GlcNAc) + ?
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?
9H-fluoren-9-ylmethyl [(3R)-1,3-dihydroxybutan-2-yl]carbamate + H2O
?
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transglycosylation
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?
alpha-NeuAc-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)[alpha-NeuAc-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)]beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-Asn + H2O
?
asialo-N-acetyl-lactosaminic-type glycoasparagine + H2O
GlcNAc-Asn + ?
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?
Asn-Tyr-Asn(GlcNAc)2(Man)3(GlcNAc)2-(Gal)2-Lys + H2O
Asn-Tyr-Asn(GlcNAc)Lys + (Gal)2(GlcNAc)2(Man)3(GlcNAc)
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?
beta-D-GlcNAc-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)]-alpha-D-Man-(1,6)]-beta-D-Man-(1-4)-beta-D-GlcNAc-(1-4)-beta-D-GlcNAc-pyridylamino
?
beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-Asn
beta-D-Man-(1->4)-beta-D-GlcNAc + beta-D-GlcNAc-Asn
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?
beta-Glcp-(1-4)-GlcpNAc-oxazoline
?
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Endo-A shows only marginal activity for transglycosylation with the disaccharide oxazoline. When used in a relatively large quantity, Endo-A can promote the transglycosylation of the disaccharide oxazoline to a GlcpNAc-Asn acceptor (it catalyzes the transfer of alpha-Manp-(1-3)-beta-Glcp-(1-4)-GlcpNAc-oxazoline to the acceptor). Endo-A promotes polymerization of beta-Glcp-(1-4)-GlcpNAc-oxazoline
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?
calf fetuin + 4-nitrophenyl-beta-D-glucoside
?
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transfer of triantennary complex type oligosaccharide, yield 3.4%
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?
carboxypeptidase Y + Lys-Val-Ala-Asn((NeuAc-Gal-GlcNAc-Man)2Man-GlcNAc2)-Lys-Thr
?
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change of hyperglycosylated protein to a sialyl complex type glycoprotein
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?
complex-type aspargine-linked oligosaccharide + H2O
?
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hydrolysis of the di-N-acetylchitobiose structure. The specificity for complex type oligosaccharides is probably defined by multiple domains in Endo D structure
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?
dansyl-Asn-(GlcNAc)2(Man)5 + H2O
dansyl-Asn-GlcNAc + ?
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?
dansyl-Lys-Val-Ala-Asn((NeuAc-Gal-GlcNAc-Man)2Man-GlcNAc2)-Lys-Thr + H2O
?
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?
dansyl-ovomucoid-glycoprotein + H2O
dansyl asparaginyl N-acetylglucosamine + ?
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?
dolichyl pyrophosphoryl oligosaccharides + H2O
?
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both glucosylated and unglucosylated polymannose oligosaccharides are released by the enzyme through cleavage of the di-N-acetylchitobiose squence
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?
fluoresceinyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
fluorescein + ?
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?
fluoresceinyl di-N-acetyl-beta-chitobioside + H2O
fluorescein + ?
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?
GlcNAc-Asn + Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc-thiazoline is a transition state mimic and is resistant to Endo-A hydrolysis
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?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + (1S)-1,5-anhydro-1-(2,4,6-trimethoxyphenyl)-D-glucitol
?
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-
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?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 1-deoxy-1-phenyl-alpha-D-gluco-2-heptulopyranose
?
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-
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?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 2-benzamido-2-deoxy-D-glucopyranose
?
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?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 2-benzoylcarbonylamino-2-deoxy-D-glucopyranose
?
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-
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-
?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 2-deoxy-2-naphthoamido-D-glucopyranose
?
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-
-
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?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 2-deoxy-2-phenoxyacetamido-D-glucopyranose
?
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-
-
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?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 2-deoxy-2-phthalimido-D-glucopyranose
?
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-
-
-
?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + benzyl beta-D-glucopyranoside
?
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-
-
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?
hen egg glycopeptide + 4-nitrophenyl-beta-D-glucose
?
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transfer of asparagine-linked oligosaccharide
-
?
human alpha1-acid glycoprotein + 4-nitrophenyl-beta-D-glucose
?
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transfer of bi-, tri- and tetraantennary complex type oligosaccharide, yield 1.7%
-
?
invertase + Lys-Val-Ala-Asn((NeuAc-Gal-GlcNAc-Man)2Man-GlcNAc2)-Lys-Thr
?
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change of hyperglycosylated protein to a sialyl complex type glycoprotein
-
?
Lys-Val-Ala-Asn((NeuAc-Gal-GlcNAc-Man)2Man-GlcNAc2)-Lys-Thr + 4-methylumbelliferyl-beta-D-N-acetylglucosaminide
?
-
-
-
-
?
Man3GlcNAc-oxazoline + Fmoc-Asn(Fucalpha(1->6)GlcNAc)-OH
?
-
-
-
?
Manalpha1-2Manalpha1-6(Manalpha1-2Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
Manalpha1-2Manalpha1-6(Manalpha1-2Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
-
-
-
-
?
Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
-
-
-
-
?
Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
?
-
-
-
-
?
Manalpha1-3Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
?
-
-
-
-
?
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
-
-
-
-
?
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
?
-
-
-
-
?
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-pyridylamino + H2O
?
-
-
-
-
?
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
?
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-