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Literature summary for 3.2.1.80 extracted from
- Expression and purification of plant fructan exohydrolases and their potential applications in fructose production (2018), Int. J. Biol. Macromol., 108, 9-17 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene FEH-III, DNA and amino acid sequence determination and analysis, FEH isozyme sequences comparisons, phylogenetic analysis, functional recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 | Helianthus tuberosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus tuberosus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the Ht1-FEH III sequence harbors four potential N-glycosylation sites | Helianthus tuberosus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Pichia pastoris strain X-33 by nickel affinity chromatography and ultrafiltration | Helianthus tuberosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-kestose + H2O | best substrate | Helianthus tuberosus | beta-D-fructose + sucrose | - |
? |  |
| fructofuranosylnystose + H2O | high activity | Helianthus tuberosus | ? | - |
? | |
| inulin + H2O | high activity | Helianthus tuberosus | ? | - |
? | |
| levan + H2O | low to moderate activity | Helianthus tuberosus | ? | - |
? | |
| additional information | substrate specificity, overview | Helianthus tuberosus | ? | - |
? | |
| nystose + H2O | - |
Helianthus tuberosus | ? | - |
? | |
| sucrose + H2O | - |
Helianthus tuberosus | D-glucose + D-fructose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64700, recombinant His-tagged enzyme, SDS-PAGE | Helianthus tuberosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ht1-FEH III | - |
Helianthus tuberosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Helianthus tuberosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 60 | over 50% of maximal activity within this range | Helianthus tuberosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Helianthus tuberosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6.5 | over 60% of maximal activity within this range | Helianthus tuberosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Helianthus tuberosus | sequence calculation | - |
6.45 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogenetic analysis of deduced amino acid sequences of plant fructan metabolism genes | Helianthus tuberosus |
| additional information | the Ht1-FEH III sequence harbors three conserved amino acid motifs (S/NDPNG, FRDP, and ECPD) and four potential N-glycosylation sites | Helianthus tuberosus |
| physiological function | the third member of the Ht1-FEH family in Jerusalem artichoke, i.e., Ht1-FEH III, not only demonstrates hydrolysis activity towards beta-(2->1)-linked fructans and beta-(2->6)-linked levan, but also towards sucrose | Helianthus tuberosus |
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