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Information on EC 3.2.1.80 - fructan beta-fructosidase
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EC Tree
3.2.1.80 fructan beta-fructosidaseIUBMB Comments
Hydrolyses inulin and levan, and also sucrose.
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Word Map
- 3.2.1.80
- hydrolases
- oligosaccharide
- xylans
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subsite
-
carbohydrate-binding
- pastoris
- cellulases
- xylanases
-
cellulolytic
- fructans
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transglycosylation
- arabinoxylans
- lignocellulose
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carbohydrate-active
- chitinases
- endoglucanases
- xylooligosaccharides
- xyloglucan
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saccharification
- endoxylanase
- avicel
- thermocellum
-
hemicellulases
- xylotriose
- cellulosome
- arabinan
-
beechwood
- cellobiohydrolases
- laminarin
-
cazymes
- lichenan
- exoglucanase
- chitosanase
-
xylanolytic
- caldicellulosiruptor
-
glycone
- agarase
-
dockerins
- spelt
-
endo-acting
- cellotetraose
- arabinofuranosidase
- levan
- mannanase
-
endo-type
- cellotriose
- cellooligosaccharides
- alpha-l-arabinofuranosidase
- fructosyltransferase
- jecorina
- synthesis
- nutrition
- diagnostics
- food industry
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
glycoside hydrolase, 1-feh, exoinulinase, fructanase, fructan exohydrolase, 6-feh, fructan 1-exohydrolase, 1-feh iia, 1-feh i, wfh-sm3, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1-FEH
1-FEH I
1-FEH IIa
6-FEH
beta-D-fructan fructanohydrolase
exo-beta-D-fructosidase
exo-beta-fructosidase
exoinulinase
FEH
fructan 1-exohydrolase
fructan 6-exohydrolase
fructan beta-fructosidase
fructan exohydrolase
Fructanase
fructofuranosidase, polysaccharide beta
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-
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inuD
inuE
inulinase
Lp6-FEHa
P-I
polysaccharide beta-fructofuranosidase
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additional information
exo-beta-D-fructosidase
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exo-beta-fructosidase
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fructan exohydrolase
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Fructanase
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1,1,1-kestose + H2O = beta-D-fructose + sucrose
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1,1,1-kestose + H2O = beta-D-fructose + sucrose
exclusive hydrolysis of beta-2-6 bonds in fructan-producing plants
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis
hydrolysis of O-glycosyl bond
hydrolysis of O-glycosyl bond
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SYSTEMATIC NAME
IUBMB Comments
beta-D-fructan fructohydrolase
Hydrolyses inulin and levan, and also sucrose.
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CAS REGISTRY NUMBER
COMMENTARY
37288-56-5
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1F,6G-di-beta-D-fructofuranosylsucrose + H2O
?
1 and 6G-kestotetraose, 57-69% activity compared to neokestose
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?
6G(1-beta-D-fructofuranosyl)2sucrose + H2O
?
i.e. 1,6G-kestotetraose, 14-15% activity compared to neokestose
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?
6G,6-kestotetraose + H2O
D-fructose + neokestose
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100%, chicorium inulin 11.9%, Aerobacter levan 8.5%, sucrose 0% rate of hydrolysis
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?
Actilight + H2O
?
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highest activity, Actilight is a commercial mixture composed of 4.8% (w/v) of free sugars (sucrose, glucose and fructose), 35.1% w/v of 1-kestose, 53% w/v of 1-nystose and 7.1% w/v of 1-fructofuranosyl-nystose
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-
?
inulobiose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulin is an intermediate product
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?
inuloheptaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulohexaose, inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products
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-
?
inulohexaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products
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?
inulopentaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulotetraose, inulotriose, inulobiose and inulin are intermediate products
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?
inulotetraose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulotriose, inulobiose and inulin are intermediate products
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?
inulotriose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulobiose and inulin are intermediate products
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?
levan + H2O
beta-D-fructofuranose + alpha-D-glucopyranose + ?
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?
levanohexaose + 6 H2O
6 beta-D-fructofuranose
levanopentaose, levanotetraose, levanotriose, levanobiose and levan are intermediate products
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?
levanopentaose + 4 H2O
5 beta-D-fructofuranose
levanotetraose, levanotriose, levanobiose and levan are intermediate products
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?
levanotetraose + 3 H2O
4 beta-D-fructofuranose
levanotriose, levanobiose and levan are intermediate products
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?
levanotriose + 2 H2O
3 beta-D-fructofuranose
levanobiose and levan are intermediate products
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?
neokestose + H2O
beta-D-fructose + sucrose
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4% activity of isoform w1 and 3% of w2 in comparison to substrate 1-kestose
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?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranose
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?
raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
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?
stachyose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranose
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?
sucrose + H2O
?
0.3% activity compared to 1-kestose
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?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
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3% enzyme activity in comparison to levan
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?
wheat graminan + H2O
?
recombinant enzyme Wfh-sm3 is able to hydrolyze all components of wheat graminan
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?
wheat graminan + H2O
beta-D-fructose + ?
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high degree of polymerization
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?
1,1,1-kestose + H2O
beta-D-fructose + sucrose
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45% enzyme activity in comparison to levan
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?
1,1,1-kestose + H2O
beta-D-fructose + sucrose
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45% enzyme activity in comparison to levan
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?
1,1-kestose + H2O
beta-D-fructose + sucrose
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39% enzyme activity in comparison to levan
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?
1,1-kestose + H2O
beta-D-fructose + sucrose
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39% enzyme activity in comparison to levan
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?
1,1-kestotetraose + H2O
?
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gene product Wfh-sm1 shows 8% activity with 1,1-kestotetraose compared to sucrose
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?
1,1-nystose + H2O
beta-D-fructose + sucrose
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72% activity of isoform w1 and 83% of w2 in comparison to substrate 1-kestose
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?
1-kestose + H2O
beta D-fructofuranose + sucrose
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100% activity of both isoforms w1 and w2
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?