Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Rhodococcus sp. M777 |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 1% (v/v) Triton X-100, 10% (v/v) glycerol, and 25% (w/v) PEG | Rhodococcus sp. M777 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nonyl-deoxynojirimycin | - |
Rhodococcus sp. M777 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GM3 ganglioside + H2O | Rhodococcus sp. M777 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. M777 | O33853 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Rhodococcus sp. M777 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-lactopyranoside + H2O | - |
Rhodococcus sp. M777 | 4-methylumbelliferol + beta-D-lactopyranose | - |
? | |
GM3 ganglioside + H2O | - |
Rhodococcus sp. M777 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer or dimer | x * 34000 + x * 25000, SDS-PAGE | Rhodococcus sp. M777 |
Synonyms | Comment | Organism |
---|---|---|
EGCII | - |
Rhodococcus sp. M777 |
endoglycoceramidase II | - |
Rhodococcus sp. M777 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Rhodococcus sp. M777 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
after 1 h at 25°C in 150mM McIlvaine's buffer (pH 5.5), the enzyme remains stable with no changes in its secondary structure and its active state | Rhodococcus sp. M777 |
42 | - |
melting temperature | Rhodococcus sp. M777 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
in 150 mM McIlvaine's buffer | Rhodococcus sp. M777 |