General Stability | Organism |
---|---|
at neutral pH and 37°C, lactase acitivity rapidly lost, partially prevented by p-chloromercuribenzoate, more stable if attached to its border-membrane | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41.7 | - |
4-O-beta-D-galactopyranosyl-D-glucopyranoside | - |
Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
brush border | - |
Rattus sp. | 5903 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-fucosyllactose + H2O | Rattus sp. | only oligosaccharides containing galactose as the glycone in beta-linkage hydrolysed | beta-D-glucose + 3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-galactopyranose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | Rattus sp. | two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis | D-galactose + beta-D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Rattus sp. | cellobiose | beta-D-glucose + D-glucose | - |
? | |
lacto-N-tetraose + H2O | Rattus sp. | - |
beta-D-glucose + ? | - |
? | |
phlorizin + H2O | Rattus sp. | only substrate that is hydrolysed at the active site for phlorizin hydrolysis | phloretin + beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 17% carbohydrate content, consisting of galactose, mannose, fucose, N-acetylglucosamine | Rattus sp. |
Purification (Comment) | Organism |
---|---|
partial | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Rattus sp. | - |
Storage Stability | Organism |
---|---|
-20°C, several months | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-fucosyllactose + H2O | only oligosaccharides containing galactose as the glycone in beta-linkage hydrolysed | Rattus sp. | beta-D-glucose + 3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-galactopyranose | - |
? | |
4-methylumbelliferyl-beta-D-galactoside + H2O | - |
Rattus sp. | 4-methylumbelliferone + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Rattus sp. | 4-nitrophenol + beta-D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis | Rattus sp. | D-galactose + beta-D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis | Rattus sp. | D-galactose + beta-D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Rattus sp. | beta-D-glucose + D-glucose | - |
? | |
lacto-N-tetraose + H2O | - |
Rattus sp. | beta-D-glucose + ? | - |
? | |
phlorizin + H2O | only substrate that is hydrolysed at the active site for phlorizin hydrolysis | Rattus sp. | phloretin + beta-D-glucose | - |
? |