Literature summary for 3.2.1.55 extracted from

  • Margolles, A.; de los Reyes-Gavillan, C.G.
    Purification and functional characterization of a novel alpha-L-arabinofuranosidase from Bifidobacterium longum B667 (2003), Appl. Environ. Microbiol., 69, 5096-5103.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, overexpression of the C-terminally His-tagged enzyme in Lactococcus lactis NZ9000 under control of nisin-inducible nisA promotor Bifidobacterium longum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Bifidobacterium longum
Hg2+
-
Bifidobacterium longum
additional information not affected by chelating or reducing agents Bifidobacterium longum
Zn2+
-
Bifidobacterium longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.295
-
4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 45°C Bifidobacterium longum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
260000
-
detagged enzyme, gel filtration Bifidobacterium longum
64000
-
4 * 64000, SDS-PAGE Bifidobacterium longum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bifidobacterium longum enzyme might have a role in the degradation of L-arabinose-containing polysaccharides together with other glycosidases ?
-
?
additional information Bifidobacterium longum B667 enzyme might have a role in the degradation of L-arabinose-containing polysaccharides together with other glycosidases ?
-
?

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum Q841V6
-
-
Bifidobacterium longum B667 Q841V6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Lactococcus lactis by Ni2+-chelate affinity chromatography Bifidobacterium longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme might have a role in the degradation of L-arabinose-containing polysaccharides together with other glycosidases Bifidobacterium longum ?
-
?
additional information substrate specificity, no endoarabinanase activity Bifidobacterium longum ?
-
?
additional information enzyme might have a role in the degradation of L-arabinose-containing polysaccharides together with other glycosidases Bifidobacterium longum B667 ?
-
?
additional information substrate specificity, no endoarabinanase activity Bifidobacterium longum B667 ?
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Bifidobacterium longum 4-nitrophenol + alpha-L-arabinofuranose
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Bifidobacterium longum B667 4-nitrophenol + alpha-L-arabinofuranose
-
?
alpha-L-arabinofuranobiose + H2O
-
Bifidobacterium longum alpha-L-arabinofuranose
-
?
alpha-L-arabinofuranobiose + H2O
-
Bifidobacterium longum B667 alpha-L-arabinofuranose
-
?
alpha-L-arabinofuranopentaose + H2O
-
Bifidobacterium longum alpha-L-arabinofuranose
-
?
alpha-L-arabinofuranopentaose + H2O
-
Bifidobacterium longum B667 alpha-L-arabinofuranose
-
?
alpha-L-arabinofuranoptetraose + H2O
-
Bifidobacterium longum alpha-L-arabinofuranose
-
?
alpha-(1->5)-L-arabinofuranotriose + H2O
-
Bifidobacterium longum alpha-L-arabinofuranose
-
?
arabinan + H2O
-
Bifidobacterium longum alpha-L-arabinose + ?
-
?
arabinoxylan + H2O
-
Bifidobacterium longum alpha-L-arabinose + ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 64000, SDS-PAGE Bifidobacterium longum

Synonyms

Synonyms Comment Organism
More enzyme belongs to the family 51 of glycosidases Bifidobacterium longum
alpha-L-arabinofuranosidase
-
Bifidobacterium longum
AbfB
-
Bifidobacterium longum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Bifidobacterium longum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70
-
Bifidobacterium longum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
half-life: 3 h Bifidobacterium longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Bifidobacterium longum

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5
-
Bifidobacterium longum