Cloned (Comment) | Organism |
---|---|
expression in Chines hamster ovary cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N124Q | 35% activity in comparison to 100% activity of wild-type enzyme | Homo sapiens |
N177Q | 48% activity in comparison to 100% activity of wild-type enzyme | Homo sapiens |
N201Q | 28% activity in comparison to 100% activity of wild-type enzyme | Homo sapiens |
N359Q | 57% activity in comparison to 100% activity of wild-type enzyme | Homo sapiens |
N385Q | 4% activity in comparison to 100% activity of wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is highly selective for terminal N-acetylgalactosamine residues | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | study of oligosaccharide structure, the first five: N124, N177, N201, N359 and N385, of six potential N-glycosylation sites are occupied | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alpha-galactosidase B | - |
Homo sapiens |
alpha-GalNAc | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
comparison of stability at 42°C of mutant and wild-type enzymes | Homo sapiens |