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Information on EC 3.2.1.49 - alpha-N-acetylgalactosaminidase

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3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.49 alpha-N-acetylgalactosaminidase

IUBMB Comments

The human lysosomal enzyme is involved in the degradation of blood type A epitope.

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3.2.1.49
n-acetylgalactosamine
sialidase
fabry
schindler
exoglycosidase
neuroaxonal
d3-binding
gcmaf
angiokeratoma
alpha-linked
beta-galnac
macrophage-activating
pomatia
corporis
diffusum
biflorus
mucin-type
medicine
alpha-n-acetylgalactosaminyltransferase
alpha-n-acetylgalactosamine
asialo
diagnostics
biotechnology

The enzyme appears in viruses and cellular organisms

Reaction Schemes

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alpha-D-GalNAc-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide

H2O

D-GalNAc

beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide

Synonyms

alpha-n-acetylgalactosaminidase, alpha-galnac, nagalase, envelope glycoprotein gp160, alpha-naga, alpha-galactosidase b, alpha-nagalase, alpha-galnacase i, alpha-nagal, alpha-galnacase ii, show all synonyms

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2-acetamido-2-deoxy-alpha-D-galactoside acetamidodeoxygalactohydrolase

4-nitrophenyl-alpha-N-acetylgalactosaminidase

AglA

Aspergillus niger

A2QL72

715130

alpha-acetylgalactosaminidase

alpha-galactosidase B

alpha-GalNAc

alpha-GalNAc-ase

alpha-GalNAcase

alpha-GalNAcase I

alpha-GalNAcase II

alpha-N acetylgalactosaminidase

Elizabethkingia meningoseptica

731531

alpha-N-acetylgalactosaminidase blood group A2 degrading enzyme

Clostridium perfringens

663810

alpha-N-galactosaminidase IV

alpha-NAGA

alpha-NAGAL

alpha-NaGalase

envelope glycoprotein gp160

2 entries

exo-alpha-N-acetylgalactosaminidase

N-acetyl-alpha-D-galactosaminidase

N-acetyl-alpha-galactosaminidase

NagA

3 entries

NaGalase

2 entries

alpha-galactosidase B

alpha-GalNAc-ase

alpha-GalNAc-ase

Aspergillus niger CCIM K2

alpha-GalNAcase I

alpha-GalNAcase I

alpha-GalNAcase II

alpha-GalNAcase II

alpha-N-galactosaminidase IV

Arenibacter latericius

654777

alpha-N-galactosaminidase IV

Arenibacter latericius KMM 426

alpha-NAGA

alpha-NAGA

alpha-NAGAL

Elizabethkingia meningoseptica

alpha-NAGAL

alpha-NAGAL

alpha-NaGalase

alpha-NaGalase

envelope glycoprotein gp160

Homo sapiens

663573

envelope glycoprotein gp160

Human immunodeficiency virus 1

663573

NagA

Elizabethkingia meningoseptica

NagA

NagA

NaGalase

NaGalase

Human immunodeficiency virus 1

663573

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

alpha-D-GalNAc-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide + H2O = D-GalNAc + beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide

6 entries

double-displacement mechanism. First step: an oxygen atom from Asp140 attacks the electrophilic C1 of the hexose ring, cleaving the glycosidic linkage and creating a covalent enzyme-substrate intermediate. The second step involves a nucleophilic attack by a water molecule, which has been deprotonated by Asp201, on the covalent intermediate.

Gallus gallus

657357

the enzyme exhibits an endo-enzyme activity in addition to an exo-activity

Homo sapiens

655748

double-displacement, or ping-pong, reaction mechanism and active site structure, overview

Homo sapiens

P17050

709530

a hydride at C3, activated by the interaction of the OH-3 hydroxyl with the conserved His228, is abstracted by NAD+ with oxidation of the OH-3 hydroxyl to a ketone. This activates H2 for proton abstraction by Tyr179, accompanied by elimination of the aglycone, thereby generating a 1,2-unsaturated intermediate. Water is then added to the anomeric center, followed by the reduction of the C3 ketone by the on-board NADH, completing the catalytic sequence and restoring NADH to NAD+ and the enzyme to its starting state to bind another substrate

Elizabethkingia meningoseptica

A4Q8F7

714006

E2 elimination following hydride transfer from C3

Elizabethkingia meningoseptica

732454

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

hydrolysis of O-glycosyl bond

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PATHWAY SOURCE

PATHWAYS

KEGG

Glycosphingolipid biosynthesis - globo and isoglobo series

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SYSTEMATIC NAME

IUBMB Comments

alpha-N-acetyl-D-galactosaminide N-acetylgalactosaminohydrolase

The human lysosomal enzyme is involved in the degradation of blood type A epitope.

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CAS REGISTRY NUMBER

COMMENTARY

9075-63-2

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

2-nitrophenyl 2-acetyl-2-deoxy-alpha-D-galactosamine + H2O

2-nitrophenol + 2-acetyl-2-deoxy-alpha-D-galactosamine

2 entries

2-nitrophenyl alpha-D-fucopyranoside + H2O

2-nitrophenol + alpha-D-fucopyranose

Homo sapiens

171228

2-nitrophenyl-alpha-D-galactoside + H2O

2-nitrophenol + alpha-D-galactose

Aspergillus niger

A2QL72

715130

2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O

2-nitrophenol + alpha-N-acetylgalactosamine

6 entries

4-methylumbelliferyl-alpha-D-galactopyranoside + H2O

4-methylumbelliferone + alpha-D-galactopyranose

2 entries

4-methylumbelliferyl-alpha-D-galactosaminide + H2O

4-methylumbelliferone + alpha-D-galactosamine

Homo sapiens

665838

4-nitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside + H2O

4-nitrophenol + 2-acetamido-2-deoxy-alpha-D-galactopyranose

Elizabethkingia meningoseptica

A4Q8F7

750729

4-nitrophenyl alpha-D-N-acetylgalactosaminide + H2O

4-nitrophenol + N-acetyl-D-galactosamine

3 entries

4-nitrophenyl alpha-N-acetylgalactosaminide + H2O

4-nitrophenol + alpha-N-acetylgalactosamine

6 entries

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

24 entries

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-galactosamine

Elizabethkingia meningoseptica

732454

4-nitrophenyl N-acetyl-beta-D-galactosamine + H2O

4-nitrophenol + N-acetyl-beta-galactosamine

Elizabethkingia meningoseptica

731531

4-nitrophenyl N-acetyl-D-galactosamine + H2O

4-nitrophenol + N-acetyl-galactosamine

2 entries

4-nitrophenyl-N-acetyl-alpha-D-galactosamide + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Clostridium perfringens

663810

blood group A glycoconjugate + H2O

2 entries

Forssman hapten + H2O

4 entries

GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide + H2O

alpha-N-acetylgalactosamine + GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide

2 entries

GalNAc-alpha1-O-Ser + H2O

Homo sapiens

665838

GalNAc-alpha1-O-Thr + H2O

Homo sapiens

665838

globotriaosylceramide + H2O

Homo sapiens

P17050

wild-type and engineered mutant enzymes

707033

o-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O

o-nitrophenol + N-acetyl-alpha-D-galactosamine

2 entries

o-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O

o-nitrophenol + N-acetyl-D-galactosamine

2 entries

ovine submaxillary asialoglycoprotein + H2O

Katsuwonus pelamis

208649

p-nitrophenyl 2-acetamide-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside + H2O

p-nitrophenol + 2-acetamide-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside

Homo sapiens

655748

p-nitrophenyl alpha-D-galactopyranoside + H2O

p-nitrophenol + alpha-D-galactopyranose

Homo sapiens

655748

p-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O

p-nitrophenol + N-acetyl-alpha-D-galactosamine

Homo sapiens

655748

p-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O

p-nitrophenol + N-acetyl-D-galactosamine

Homo sapiens

663573

p-nitrophenyl-2-deoxy-alpha-D-galactopyranoside + H2O

p-nitrophenol + 2-deoxy-alpha-D-galactopyranose

Homo sapiens

171228

p-nitrophenyl-alpha-N-acetyl-D-galactoaminide + H2O

p-nitrophenol + alpha-N-acetyl-D-galactosamine

Homo sapiens

the Hep-G2 cell lysate hydrolyzes the exo-type substrate

655254

p-nitrophenyl-alpha-N-acetyl-D-galactosaminide + H2O

p-nitrophenol + alpha-N-acetyl-D-galactosamine

2 entries

p-nitrophenyl-alpha-N-acetylgalactosaminide + H2O

p-nitrophenol + alpha-N-acetylgalactosamine

3 entries

p-nitrophenyl-N-acetyl-alpha-D-galactosaminide + H2O

p-nitrophenol + N-acetyl-alpha-D-galactosamine

2 entries

phenyl-alpha-N-acetylglucosaminide + H2O

N-acetylgalactosamine + phenol

Bos taurus

208666

serum vitamin D3-binding protein + H2O

3 entries

additional information

25 entries

2-nitrophenyl 2-acetyl-2-deoxy-alpha-D-galactosamine + H2O

2-nitrophenol + 2-acetyl-2-deoxy-alpha-D-galactosamine

Aspergillus niger

G5D7B5

716839

2-nitrophenyl 2-acetyl-2-deoxy-alpha-D-galactosamine + H2O

2-nitrophenol + 2-acetyl-2-deoxy-alpha-D-galactosamine

Aspergillus niger CCIM K2

G5D7B5

716839

2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O

2-nitrophenol + alpha-N-acetylgalactosamine

Aspergillus niger

G5D7B5

716839

2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O

2-nitrophenol + alpha-N-acetylgalactosamine

Aspergillus niger

A2QL72

715130

2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O

2-nitrophenol + alpha-N-acetylgalactosamine

Aspergillus niger

enzyme acts with retention of the anomeric configuration

695797

2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O

2-nitrophenol + alpha-N-acetylgalactosamine

Aspergillus niger CCIM K2

enzyme acts with retention of the anomeric configuration

695797

2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O

2-nitrophenol + alpha-N-acetylgalactosamine

Aspergillus niger CCIM K2

G5D7B5

716839

2-nitrophenyl-alpha-N-acetylgalactosaminide + H2O

2-nitrophenol + alpha-N-acetylgalactosamine

Elizabethkingia meningoseptica

A4Q8F7

714006

4-methylumbelliferyl-alpha-D-galactopyranoside + H2O

4-methylumbelliferone + alpha-D-galactopyranose

Homo sapiens

171227, 208660

4-methylumbelliferyl-alpha-D-galactopyranoside + H2O

4-methylumbelliferone + alpha-D-galactopyranose

Homo sapiens

P17050

substrate of engineered enzyme mutant S188E/A191L, not of the wild-type enzyme

707033

4-nitrophenyl alpha-D-N-acetylgalactosaminide + H2O

4-nitrophenol + N-acetyl-D-galactosamine

Architeuthis sp.

707632

4-nitrophenyl alpha-D-N-acetylgalactosaminide + H2O

4-nitrophenol + N-acetyl-D-galactosamine

Homo sapiens

707963

4-nitrophenyl alpha-D-N-acetylgalactosaminide + H2O

4-nitrophenol + N-acetyl-D-galactosamine

Homo sapiens

P17050

substrate binding and active site structure, active-site interactions and ligand binding, overview. Inactive enzyme conformation, overview

709530

4-nitrophenyl alpha-N-acetylgalactosaminide + H2O

4-nitrophenol + alpha-N-acetylgalactosamine

Acremonium sp.

208648

4-nitrophenyl alpha-N-acetylgalactosaminide + H2O

4-nitrophenol + alpha-N-acetylgalactosamine

Katsuwonus pelamis

208649

4-nitrophenyl alpha-N-acetylgalactosaminide + H2O

4-nitrophenol + alpha-N-acetylgalactosamine

Patella vulgata

208661

4-nitrophenyl alpha-N-acetylgalactosaminide + H2O

4-nitrophenol + alpha-N-acetylgalactosamine

Solenocera melantho

208650

4-nitrophenyl alpha-N-acetylgalactosaminide + H2O

4-nitrophenol + alpha-N-acetylgalactosamine

Sus scrofa

208656, 208665

4-nitrophenyl alpha-N-acetylgalactosaminide + H2O

4-nitrophenol + alpha-N-acetylgalactosamine

Todarodes pacificus

208652

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Arenibacter latericius

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Arenibacter latericius KMM 3523

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Arenibacter latericius KMM 3528

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Arenibacter palladensis

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Arenibacter palladensis KMM 3961

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Arenibacter palladensis KMM 3980

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Arenibacter sp. KMM 6041

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Aspergillus niger

G5D7B5

716839

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Aspergillus niger CCIM K2

G5D7B5

716839

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Cellulophaga sp.

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Cellulophaga sp. KMM 6483

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Cellulophaga sp. KMM 6488

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Formosa sp. KMM 6408

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Kriegella aquimaris

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Kriegella aquimaris KMM 3942

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Maribacter sp.

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Maribacter sp. KMM 3952

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Maribacter sp. KMM 6484

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Maribacter sp. KMM 6485

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Maribacter sp. KMM 6486

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Maribacter sp. KMM 6487

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Maribacter sp. KMM 6489

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Zobellia laminariae

732464

4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O

4-nitrophenol + N-acetyl-alpha-D-galactosamine

Zobellia laminariae KMM 6205

732464

4-nitrophenyl N-acetyl-D-galactosamine + H2O

4-nitrophenol + N-acetyl-galactosamine

Homo sapiens

P17050

751448, 754583

4-nitrophenyl N-acetyl-D-galactosamine + H2O

4-nitrophenol + N-acetyl-galactosamine

Homo sapiens

754583

blood group A glycoconjugate + H2O

Clostridium perfringens

alpha-N-acetylgalactosaminidase blood group A2 degrading activity, substrate from human erythrocytes and plasma

663810

blood group A glycoconjugate + H2O

Clostridium perfringens

alpha-N-acetylgalactosaminidase blood group A2 degrading activity, substrate from human and murine erythrocytes and plasma

663810

Forssman hapten + H2O

Homo sapiens

208660

Forssman hapten + H2O

Patella vulgata

208661

Forssman hapten + H2O

Sus scrofa

208656

Forssman hapten + H2O

[Ruminococcus] torques

208673

GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide + H2O

alpha-N-acetylgalactosamine + GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide

Asterina amurensis

i.e. Forssman hapten glycolipid, production of globoside by both isozymes

707791

GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide + H2O

alpha-N-acetylgalactosamine + GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide

Homo sapiens

i.e. Forssman hapten glycolipid

707791

o-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O

o-nitrophenol + N-acetyl-alpha-D-galactosamine

Clostridium perfringens

655755

o-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O

o-nitrophenol + N-acetyl-alpha-D-galactosamine

Clostridium perfringens

substrate from the blood type A2 antigen producing H antigen, blood type O

657266

o-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O

o-nitrophenol + N-acetyl-D-galactosamine

Gallus gallus

208669

o-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O

o-nitrophenol + N-acetyl-D-galactosamine

Homo sapiens

208660

p-nitrophenyl-alpha-N-acetyl-D-galactosaminide + H2O

p-nitrophenol + alpha-N-acetyl-D-galactosamine

Arenibacter latericius

654777

p-nitrophenyl-alpha-N-acetyl-D-galactosaminide + H2O

p-nitrophenol + alpha-N-acetyl-D-galactosamine

Arenibacter latericius KMM 426

654777

p-nitrophenyl-alpha-N-acetylgalactosaminide + H2O

p-nitrophenol + alpha-N-acetylgalactosamine

Aspergillus niger

208674

p-nitrophenyl-alpha-N-acetylgalactosaminide + H2O

p-nitrophenol + alpha-N-acetylgalactosamine

Gallus gallus

208671

p-nitrophenyl-alpha-N-acetylgalactosaminide + H2O

p-nitrophenol + alpha-N-acetylgalactosamine

[Ruminococcus] torques

208673

p-nitrophenyl-N-acetyl-alpha-D-galactosaminide + H2O

p-nitrophenol + N-acetyl-alpha-D-galactosamine

Clostridium perfringens

208655, 655755, 657266

p-nitrophenyl-N-acetyl-alpha-D-galactosaminide + H2O

p-nitrophenol + N-acetyl-alpha-D-galactosamine

Clostridium perfringens

Q8XNK8

655609

serum vitamin D3-binding protein + H2O

Homo sapiens

i.e. Gc protein, human substrate, deglycosylation of the substrate

663573

serum vitamin D3-binding protein + H2O

Human immunodeficiency virus 1

i.e. Gc protein, the human substrate is the precursor of the principal macrophage activating factor, MAF, the deglycosylated prrecursor cannot be converted, which leads to immunosuppression

663573

serum vitamin D3-binding protein + H2O

Human immunodeficiency virus 1

i.e. Gc protein, human substrate, deglycosylation of the substrate

663573

additional information

Aspergillus niger

the enzyme glycosylizes free serine and threonine residues in the native and the N-tert-butoxycarbonyl-protected analogue protein to gain the Tn antigen. The enzyme catalyzes glycosylations yielding to a series of 2-acetamido-2-deoxy-alpha-D-galactobiosides using 2-acetamido-2-deoxy-D-galactopyranose as a glycosyl donor, identification of isomers alpha-D-GalpNAc-1,6-D-GalpNAc, alpha-D-GalpNAc-1,3-D-GalpNAc, and alpha-D-GalpNAc-1,6-D-GalfNAc

707347

additional information

Aspergillus niger

A2QL72

preference for alpha-N-acetyl-D-galactosaminide over alpha-D-galactoside. alpha-N-acetylgalactosaminidase activity uses a retaining mechanism

715130

additional information

Aspergillus niger

G5D7B5

an exoglycosidase specific for the hydrolysis of terminal alpha-linked N-acetylgalactosamine in various sugar chains

716839

additional information

Aspergillus niger

G5D7B5

the enzyme also exhibits alpha-galactosidase activity

716839

additional information

Aspergillus niger CCIM K2

G5D7B5

an exoglycosidase specific for the hydrolysis of terminal alpha-linked N-acetylgalactosamine in various sugar chains

716839

additional information

Aspergillus niger CCIM K2

G5D7B5

the enzyme also exhibits alpha-galactosidase activity

716839

additional information

Asterina amurensis

the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins

707791

additional information

Clostridium perfringens

the enzyme hydrolyzes the terminal N-actyl-alpha-D-galactosamine from the blood type A2 antigen producing H antigen, blood type O

655075

additional information

Clostridium perfringens

the enzyme is highly selective for terminal N-acetylgalactosamine residues, sugars other than N-acetyl-alpha-D-galactosaminide are no substrates

657266

additional information

Clostridium perfringens

the enzyme is highly selective for terminal N-acetylgalactosamine residues, sugars other than N-acetyl-alpha-D-galactosaminide are not substrates. the enzyme degrades blood A epitope

655755

additional information

Elizabethkingia meningoseptica

A4Q8F7

enzyme efficiently cleaves blood group A antigen. Unusual catalytic mechanism involving NAD+. NagA might adopt a mechanism similar to that of glycosyl hydrolase family 4 enzymes utilizing an oxidation-elimination-addition-reduction sequence

714006

additional information

Elizabethkingia meningoseptica

the enzyme catalyses the hydrolysis of aryl 2-acetamido-2-deoxy-alpha-D-galactopyranosides

732454

additional information

Homo sapiens

Gal-beta-GalNAc-alpha-PNP is no substrate for Hep-G2 cell lysate, the enzyme of tumor cell lysate reduces the macrophage activing potency of GcMAF

655254

additional information

Homo sapiens

the enzyme inactivates the macrophage activing factor produced from Gc protein and this results in reduced phagocytic activity and superoxide-generation capacity of monocytes/macrophages

655748

additional information

Homo sapiens

the enzyme is highly selective for terminal N-acetylgalactosamine residues

655678

additional information

Homo sapiens

enzyme deficiency, due to missense mutations at residue R329 causing structural changes in the enzyme leading to differing substrate specificity, causes the Kanzaki disease, an autosomal recessine storage disorder with accumulation of GalNAcalpha1-O-Ser/Thr in the lysosome and increased urinary excretion of O-linked sialylglycopeptides, phenotype, overview

665838

additional information

Homo sapiens

P17050

alpha-NAGAL is a lysosomal exoglycosidase that cleaves terminal alpha-N-acetylgalactosamine residues from glycopeptides and glycolipids

709530

additional information

Homo sapiens

alpha-NAGAL is a lysosomal exoglycosidase that cleaves terminal alpha-N-acetylgalactosamine residues from glycopeptides and glycolipids

709530

additional information

Homo sapiens

the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins

707791

additional information

Homo sapiens

P17050

Ser188 and Ala191 play important roles in the recognition of an N-acetylgalactosamine residue in NAGA

707033

additional information

Homo sapiens

Ser188 and Ala191 play important roles in the recognition of an N-acetylgalactosamine residue in NAGA

707033

additional information

Homo sapiens

P17050

the enzyme also shows activity with 4-nitrophenyl alpha-D-galactosaminide

709530

additional information

Homo sapiens

the enzyme also shows activity with 4-nitrophenyl alpha-D-galactosaminide

709530

additional information

Human immunodeficiency virus 1

the enzyme plays a dual role in regulating both infectivity and immunosuppression

663573

additional information

Human immunodeficiency virus 1

the HIV-1 viral envelope glycoprotein gp160 shows alpha-N-galactosaminidase activity

663573

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

blood group A glycoconjugate + H2O

Clostridium perfringens

alpha-N-acetylgalactosaminidase blood group A2 degrading activity, substrate from human erythrocytes and plasma

663810

serum vitamin D3-binding protein + H2O

Human immunodeficiency virus 1

i.e. Gc protein, the human substrate is the precursor of the principal macrophage activating factor, MAF, the deglycosylated prrecursor cannot be converted, which leads to immunosuppression

663573

additional information

8 entries

additional information

Aspergillus niger

G5D7B5

an exoglycosidase specific for the hydrolysis of terminal alpha-linked N-acetylgalactosamine in various sugar chains

716839

additional information

Aspergillus niger CCIM K2

G5D7B5

an exoglycosidase specific for the hydrolysis of terminal alpha-linked N-acetylgalactosamine in various sugar chains

716839

additional information

Asterina amurensis

the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins

707791

additional information

Homo sapiens

665838

additional information

Homo sapiens

P17050

alpha-NAGAL is a lysosomal exoglycosidase that cleaves terminal alpha-N-acetylgalactosamine residues from glycopeptides and glycolipids

709530

additional information

Homo sapiens

alpha-NAGAL is a lysosomal exoglycosidase that cleaves terminal alpha-N-acetylgalactosamine residues from glycopeptides and glycolipids

709530

additional information

Homo sapiens

the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins

707791

additional information

Human immunodeficiency virus 1

the enzyme plays a dual role in regulating both infectivity and immunosuppression

663573

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

NAD+

Elizabethkingia meningoseptica

A4Q8F7

binds 1 NAD+ per subunit. The NAD+ cannot dissociate

714006

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Ca2+

Asterina amurensis

slight activation of both isozymes

707791

Cu2+

Asterina amurensis

slight activation of both isozymes

707791

K+

Asterina amurensis

slight activation of both isozymes

707791

Mg2+

2 entries

Na+

Asterina amurensis

slight activation of both isozymes

707791

Zn2+

Asterina amurensis

slight activation of both isozymes

707791

Mg2+

Arenibacter latericius

activates

654777

Mg2+

Asterina amurensis

slight activation of both isozymes

707791

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2,2'-dianisidine

Arenibacter latericius

2 mM, 70% of original activity

654777

Acetylimidazole

Arenibacter latericius

5 mM, 30% of original activity

654777

Ag+

5 entries

Ca2+

2 entries

carbodiimide

Arenibacter latericius

1.8 mM, complete loss of activity

Cu2+

D-galactose

D-glucose

diethylpyrocarbonate

Arenibacter latericius

2 mM, 60% of original activity

Fe2+

Hg2+

K+

Mg2+

Mn2+

N-acetyl-D-galactosamine

2 entries

N-acetyl-D-glucosamine

Homo sapiens

707791

N-acetylgalactosamine

4 entries

N-bromosuccinimide

Arenibacter latericius

1.8 mM, complete loss of activity

654777

N-ethylmaleimide

Arenibacter latericius

1.8 mM, 60% of original activity

Na+

NaN3

p-chloromercuribenzoate

Arenibacter latericius

1.8 mM, complete loss of activity

654777

Pb2+

Aspergillus niger

10 mM, 30% residual activity

SDS

Zn2+

additional information

2 entries

Ag+

Asterina amurensis

moderate inhibition of isozyme alpha-GalNAcase I, strong inhibition of isozyme alpha-GalNAcase II

Ag+

Ag+

Ag+

Ag+

isoenzyme II

Ca2+

Arenibacter latericius

1 mM, complete loss of activity

Ca2+

Cu2+

Cu2+

isoenzyme II

Cu2+

[Ruminococcus] torques

208673

D-galactose

Asterina amurensis

about 10% inhibition of isozymes alpha-GalNAcase I and isozyme alpha-GalNAcase II

D-galactose

D-galactose

D-galactose

D-galactose

isoenzyme I

Hg2+

Hg2+

10 mM, 12% residual activity

695797

Hg2+

Asterina amurensis

slight inhibition of isozyme alpha-GalNAcase I, strong inhibition of isozyme alpha-GalNAcase II

707791

Hg2+

Clostridium perfringens

Hg2+

Hg2+

Hg2+

isoenzyme II

Hg2+

[Ruminococcus] torques

208673

N-acetyl-D-galactosamine

Asterina amurensis

57% and 62% inhibition of isozyme alpha-GalNAcase I and isozyme alpha-GalNAcase II

707791

N-acetyl-D-galactosamine

Homo sapiens

707791

N-acetylgalactosamine

Patella vulgata

208661

N-acetylgalactosamine

Solenocera melantho

208650

N-acetylgalactosamine

Sus scrofa

208665

N-acetylgalactosamine

NaN3

NaN3

additional information

Aspergillus niger

not inhibitory: Ca2+, Mg2+, Mn2+, Ag+

695797

additional information

Asterina amurensis

no inhibition by D-glucose, N-acetyl-D-glucosamine, and Mn2+

707791

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

All organisms
Acremonium sp.
Architeuthis sp.
Arenibacter latericius
Arenibacter latericius KMM 3523
Arenibacter latericius KMM 3528
Arenibacter latericius KMM 426
Arenibacter palladensis
Arenibacter palladensis KMM 3961
Arenibacter palladensis KMM 3980
Arenibacter sp. KMM 6041
Aspergillus niger
Aspergillus niger CCIM K2
Asterina amurensis
Bacteroides fragilis
Bos taurus
Butyrivibrio fibrisolvens
Cellulophaga sp.
Cellulophaga sp. KMM 6483
Cellulophaga sp. KMM 6488
Clostridium perfringens
Elizabethkingia meningoseptica
Entodinium caudatum
Entodinium simplex
Epidinium caudatum
Eremoplastron bovis
Eudiplodinium maggii
Fibrobacter succinogenes
Formosa sp. KMM 6408
Gallus gallus
Helix pomatia
Homo sapiens
Human immunodeficiency virus 1
Katsuwonus pelamis
Kriegella aquimaris
Kriegella aquimaris KMM 3942
Lachnospira multipara
Maribacter sp.
Maribacter sp. KMM 3952
Maribacter sp. KMM 6484
Maribacter sp. KMM 6485
Maribacter sp. KMM 6486
Maribacter sp. KMM 6487
Maribacter sp. KMM 6489
Megasphaera elsdenii
Mus musculus
Patella vulgata
Ruminobacter amylophilus
Ruminococcus albus
Ruminococcus flavefaciens
Selenomonas ruminantium
Solenocera melantho
Streptococcus equinus
Succinivibrio dextrinosolvens
Sus scrofa
Todarodes pacificus
Zobellia laminariae
Zobellia laminariae KMM 6205
[Ruminococcus] torques