Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermoanaerobacterium xylanolyticum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 15% (w/v) PEG 6000, 0.1 M Bis-Tris propane, pH 8.0 | Thermoanaerobacterium xylanolyticum |
Protein Variants | Comment | Organism |
---|---|---|
D508H | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
D508N | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
D593A | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
E441A | the mutant shows strongly reduced activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
R544W | the mutant shows slightly increased activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
R786H | the mutant shows reduced activity compared to the wild type enzyme | Thermoanaerobacterium xylanolyticum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-dinitrophenyl 2-deoxy-2-fluoro-beta-D-glucoside | - |
Thermoanaerobacterium xylanolyticum | |
deoxynojirimycin | - |
Thermoanaerobacterium xylanolyticum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
0.21 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme with N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
0.25 | - |
cellobiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
0.26 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R544W, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
0.27 | - |
laminaribiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
15.7 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R786H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
16.3 | - |
4-nitrophenyl beta-D-galactoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
41 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508N, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
110 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucosylceramide + H2O | Thermoanaerobacterium xylanolyticum | - |
beta-D-glucose + ceramide | - |
? | |
glucosylceramide + H2O | Thermoanaerobacterium xylanolyticum GH116 | - |
beta-D-glucose + ceramide | - |
? | |
glucosyllysosphingosine + H2O | Thermoanaerobacterium xylanolyticum | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium xylanolyticum | F6BL85 | - |
- |
Thermoanaerobacterium xylanolyticum GH116 | F6BL85 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon cobalt affinity resin column chromatography, HisTrap column chromatography, and Superdex 200 gel filtration | Thermoanaerobacterium xylanolyticum |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0817 | - |
with methyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
0.123 | - |
with 4-nitrophenyl N-acetyl-beta-D-glucosaminide as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
2.23 | - |
with gentibiose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.58 | - |
with n-octyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
3.74 | - |
with n-heptyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
4.1 | - |
with sophorose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
8.9 | - |
with 4-nitrophenyl beta-D-galactoside as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
11.7 | - |
with laminaribiose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
13.4 | - |
with cellobiose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
13.5 | - |
with cellohexaose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
13.8 | - |
with cellopentaose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
14.9 | - |
with cellotetraose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
15 | - |
with laminaritetraose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
15.6 | - |
with cellotriose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
16.1 | - |
with laminaritriose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
16.5 | - |
with laminaripentaose as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
27.3 | - |
with 4-nitrophenyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-cellobioside + H2O | 1.1% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | 33% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | 33% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum GH116 | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | 100% activity | Thermoanaerobacterium xylanolyticum | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | 0.45% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
cellobiose + H2O | 49% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
cellohexaose + H2O | 49% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
cellotetraose + H2O | 55% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
cellotriose + H2O | 57% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
cellotriose + H2O | 57% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum GH116 | ? | - |
? | |
gentibiose + H2O | 8.2% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
glucosylceramide + H2O | - |
Thermoanaerobacterium xylanolyticum | beta-D-glucose + ceramide | - |
? | |
glucosylceramide + H2O | - |
Thermoanaerobacterium xylanolyticum GH116 | beta-D-glucose + ceramide | - |
? | |
glucosyllysosphingosine + H2O | - |
Thermoanaerobacterium xylanolyticum | ? | - |
? | |
laminaribiose + H2O | 43% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | 2 D-glucose | - |
? | |
laminaripentaose + H2O | 60% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
laminaritetraose + H2O | 55% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
laminaritriose + H2O | 59% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
laminaritriose + H2O | 59% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum GH116 | ? | - |
? | |
methyl beta-D-glucoside + H2O | 0.3% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | methanol + D-glucose | - |
? | |
additional information | the enzyme shows less than 0.04% activity with 4-nitrophenyl alpha-D-galactoside, 4-nitrophenyl alpha-L-arabinoside, 4-nitrophenyl beta-D-fucoside, 4-nitrophenyl alpha-D-glucoside, 4-nitrophenyl beta-D-mannoside, and 4-nitrophenyl beta-D-xyloside | Thermoanaerobacterium xylanolyticum | ? | - |
? | |
additional information | the enzyme shows less than 0.04% activity with 4-nitrophenyl alpha-D-galactoside, 4-nitrophenyl alpha-L-arabinoside, 4-nitrophenyl beta-D-fucoside, 4-nitrophenyl alpha-D-glucoside, 4-nitrophenyl beta-D-mannoside, and 4-nitrophenyl beta-D-xyloside | Thermoanaerobacterium xylanolyticum GH116 | ? | - |
? | |
n-heptyl beta-D-glucoside + H2O | 14% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | heptanol + D-glucose | - |
? | |
n-octyl beta-D-glucoside + H2O | 13% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | octanol + D-glucose | - |
? | |
sophorose + H2O | 15% activity compared to 4-nitrophenyl beta-D-glucoside | Thermoanaerobacterium xylanolyticum | 2 D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase | - |
Thermoanaerobacterium xylanolyticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermoanaerobacterium xylanolyticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
81.3 | - |
melting temperature | Thermoanaerobacterium xylanolyticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.47 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
30.2 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508N, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
37.6 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme with N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
41.4 | - |
laminaribiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
44.4 | - |
cellobiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
48.2 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R544W, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
49 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
193 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R786H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
255 | - |
4-nitrophenyl beta-D-galactoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Thermoanaerobacterium xylanolyticum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
0.74 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D508N, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
12.3 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R786H, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
15.6 | - |
4-nitrophenyl beta-D-galactoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
153 | - |
laminaribiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
178 | - |
cellobiose | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
179 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme with N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
186 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme R544W, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum | |
272 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme without N-terminal tag, at pH 5.5 and 60°C | Thermoanaerobacterium xylanolyticum |