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Information on EC 3.2.1.45 - glucosylceramidase
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3.2.1.45 glucosylceramidaseIUBMB Comments
Also acts on glucosylsphingosine (cf. EC 3.2.1.62 glycosylceramidase).
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Word Map
- 3.2.1.45
- gaucher
- parkinsonism
- spleen
- marrow
- gcase
-
neuronopathic
- chitotriosidase
-
saposins
- visceral
-
hematological
-
jewish
-
non-neuronopathic
- sphingolipids
-
ashkenazi
- thrombocytopenia
- glycolipids
- dementia
-
splenectomy
- anemia
- platelet
- splenomegaly
-
hepatosplenomegaly
- glycosphingolipids
-
autophagy-lysosomal
-
non-motor
- corneum
- synucleinopathies
- genzyme
-
conduritol
-
organomegaly
- alpha-synuclein
- ambroxol
- medicine
- hepatomegaly
- fabry
-
splenectomized
-
iminosugars
- krabbe
- taurocholate
- niemann-pick
-
stratum
- prosaposin
- biotechnology
-
non-jewish
- sphingomyelinase
-
atp13a2
- deoxynojirimycin
-
hypersplenism
- fetalis
-
cytopenia
-
reticuloendothelial
-
4-methylumbelliferyl
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
glucocerebrosidase, imiglucerase, acid beta-glucosidase, beta-glucocerebrosidase, glucosylceramidase, alglucerase, velaglucerase alfa, taliglucerase alfa, cerezyme, glccerase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acid beta-glucocerebrosidase
acid beta-glucosidase
acid-beta-glucosidase
Alglucerase
-
-
-
-
beta-D-glucocerebrosidase
-
-
-
-
beta-glucocerebrosidase
beta-glucosidase
beta-glucosidase 2
beta-glucosylceramidase
-
-
-
-
bile acid beta-glucosidase
ceramidase, glucosyl-
-
-
-
-
ceramide glucosidase
-
-
-
-
cerebroside beta-glucosidase
-
-
-
-
Ceredase
Cerezyme
D-glucosyl-N-acylsphingosine glucohydrolase
GBA
GBA1
GBA2
Gcase
glcCer-beta-glucosidase
-
-
-
-
GlcCerase
glucocerebrosidase
glucocerebroside beta-glucosidase
-
-
-
-
glucose cerebrosidase
-
-
-
-
glucosphingosine glucosylhydrolase
-
-
-
-
glucosylceramide beta-glucosidase
glucosylcerebrosidase
-
-
-
-
glucosylsphingosine beta-D-glucosidase
-
-
-
-
glucosylsphingosine beta-glucosidase
-
-
-
-
Imiglucerase
microsomal bile acid beta-glucosidase
non-lysosomal glucosylceramidase
psychosine hydrolase
-
-
-
-
velaglucerase alfa
acid beta-glucosidase
-
-
-
-
beta-glucocerebrosidase
-
-
-
-
beta-glucocerebrosidase
-
-
D-glucosyl-N-acylsphingosine glucohydrolase
-
-
-
-
Gcase
-
-
glucocerebrosidase
-
-
-
-
glucocerebrosidase
-
-
glucosylceramide beta-glucosidase
-
-
-
-
Imiglucerase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a D-glucosyl-N-acylsphingosine + H2O = D-glucose + a ceramide
-
-
-
-
a D-glucosyl-N-acylsphingosine + H2O = D-glucose + a ceramide
the enzyme hydrolyses the beta-glucosidic linkage of glucosylceramide with retention of anomeric conformation via a two-step, double-displacement mechanism
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
D-glucosyl-N-acylsphingosine glucohydrolase
Also acts on glucosylsphingosine (cf. EC 3.2.1.62 glycosylceramidase).
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CAS REGISTRY NUMBER
COMMENTARY
37228-64-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide + H2O
(6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)sphingosine + D-glucose
-
-
-
-
?
12(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminododecanoyl-2-N-sphingosyl 1-O-beta-D-glucoside + H2O
D-glucose + 12(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminododecanoyl-2-N-sphingosine
2,4-dinitrophenyl beta-D-glucopyranoside + H2O
p-nitrophenol + beta-D-glucose
-
-
-
-
?
3-amido-(Black Hole Quencher 2)-6-methoxytriazolyl-1'-ethyl-3'-amido-(boron-dipyrromethene 576/589) beta-D-glucopyranoside + H2O
6-methoxytriazolyl-1'-ethyl-3'-amido-(boron-dipyrromethene 576/589) beta-D-glucopyranoside + Black Hole Quencher 2-N-(3-hydroxypropyl)butanamide
-
-
-
?
3-amido-[4-[[4-(dimethylamino)phenyl]azo]-benzoic acid]-L-6-methoxytriazol-5-[(2-azidoethyl)amino]naphthalene-1-sulfonic acid beta-D-glucopyranoside + H2O
6-O-[(1-[2-[(5-sulfonaphthalen-1-yl)amino]ethyl]-1H-1,2,3-triazol-4-yl)methyl]-beta-D-glucopyranose + 4-[(Z)-[4-(dimethylamino)phenyl]diazenyl]-N-(3-hydroxypropyl)benzamide
-
-
-
?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucopyranose
4-methylumbelliferyl beta-D-glucoside
4-methylumbelliferone + beta-D-glucose
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
4-methylumbelliferone + beta-D-glucose
-
-
-
?
4-methylumbelliferyl glucoside + H2O
4-methylumbelliferone + D-glucose
-
-
-
-
?
4-methylumbelliferyl-beta-D-galactopyranoside + H2O
methylumbelliferone + beta-D-galactose
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
methylumbelliferone + beta-D-glucose
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O
4-methylumbelliferone + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-cellobioside + H2O
4-nitrophenol + cellobiose
1.1% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
100% activity
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
4-undecylumbelliferyl beta-D-glucopyranoside + H2O
4-undecylumbelliferone + D-glucose
-
-
-
-
?
5-(pentafluorobenzoylamino)-fluorescein di-beta-D-glucopyranoside + H2O
5-(pentafluorobenzoylamino)-fluorescein + 2 beta-D-glucopyranose
-
-
-
?
5-(pentafluorobenzoylamino)fluorescein di-beta-D-glucopyranoside + H2O
5-(pentafluorobenzoylamino)fluorescein + 2 beta-D-glucopyranose
-
-
-
?
6(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminohexyl-2-N-sphingosyl 1-O-beta-D-glucoside + H2O
D-glucose + 6(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminohexyl-2-N-sphingosine
6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-galactosylceramide + H2O
6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)ceramide + D-galactose
-
-
-
-
?
cellobiose + H2O
?
49% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
cellohexaose + H2O
?
49% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
cellotetraose + H2O
?
55% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
D-glucosyl-beta-(1-1')-N-dodecanoyl-D-erythro-sphingosine + H2O
D-glucose + N-dodecanoyl-D-erythro-sphingosine
-
product and internal standard are quantified using tandem mass spectrometry
-
-
?
gentibiose + H2O
?
8.2% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
heptylumbelliferyl-beta-D-glucopyranoside + H2O
heptylumbelliferone + glucose
-
-
-
-
?
laminaribiose + H2O
2 D-glucose
43% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
laminaripentaose + H2O
?
60% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
laminaritetraose + H2O
?
55% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
methyl beta-D-glucoside + H2O
methanol + D-glucose
0.3% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
N-(6-(7-nitrobenzo-2-oxa-1,3-diazol-4-yl)amino)hexanoyl-D-erythro-glucosylsphingosine + H2O
D-glucose + N-(6-(7-nitrobenzo-2-oxa-1,3-diazol-4-yl)amino)hexanoyl-sphingosine
-
-
-
?
n-heptyl beta-D-glucoside + H2O
heptanol + D-glucose
14% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
n-octyl beta-D-glucoside + H2O
octanol + D-glucose
13% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
N-palmitoyl-dihydroglucocerebroside + H2O
D-glucose + N-palmitoyl-dihydrocerebroside
-
-
-
-
?
N-stearoyl-dihydroglucocerebroside + H2O
D-glucose + N-stearoyl-dihydrocerebroside
-
-
-
-
?
p-nitrophenyl beta-D-glucopyranoside + H2O
p-nitrophenol + beta-D-glucose
-
-
-
-
?
sophorose + H2O
2 D-glucose
15% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
undecylumbelliferyl-beta-D-glucopyranoside + H2O
undecylumbelliferone + glucose
-
-
-
-
?
12(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminododecanoyl-2-N-sphingosyl 1-O-beta-D-glucoside + H2O
D-glucose + 12(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminododecanoyl-2-N-sphingosine
-
-
-
-
?
12(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminododecanoyl-2-N-sphingosyl 1-O-beta-D-glucoside + H2O
D-glucose + 12(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminododecanoyl-2-N-sphingosine
-
-
-
?
3-O-benzoyl-N-stearylglucosphingosine + H2O
D-glucose + 3-O-benzoyl-N-stearylsphingosine
-
not: galactocerebroside
-
-
?
3-O-benzoyl-N-stearylglucosphingosine + H2O
D-glucose + 3-O-benzoyl-N-stearylsphingosine
-
not: galactocerebroside
-
-
?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucopyranose
-
-
-
-
?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucopyranose
-
-
-
?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucopyranose
-
-
-
-
?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucopyranose
-
-
-
?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucopyranose
-
-
-
?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucopyranose
-
-
-
?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucopyranose
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucose
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
4-methylumbelliferone + beta-D-glucose
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
methylumbelliferone + glucose
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
methylumbelliferone + glucose
-
-
136450, 136452, 136453, 136456, 136459, 136462, 136464, 136465, 136470, 136471, 136473, 136475, 136476, 136477, 136478, 136479, 136483, 136484, 682548, 697328
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
methylumbelliferone + glucose
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
methylumbelliferone + glucose
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
methylumbelliferone + glucose
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
methylumbelliferone + glucose
-
-
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
33% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
33% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
0.45% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
6(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminohexyl-2-N-sphingosyl 1-O-beta-D-glucoside + H2O
D-glucose + 6(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminohexyl-2-N-sphingosine
-
NBD-glucocerebroside
-
-
?
6(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminohexyl-2-N-sphingosyl 1-O-beta-D-glucoside + H2O
D-glucose + 6(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl))aminohexyl-2-N-sphingosine
-
complexed with bovine serum albumin
-
-
?
cellotriose + H2O
?
57% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
cellotriose + H2O
?
57% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
glucocerebroside + H2O
glucose + ceramide
-
-
-
-
?
glucocerebroside + H2O
glucose + ceramide
-
-
136450, 136452, 136453, 136456, 136458, 136459, 136462, 136463, 136464, 136465, 136466, 136469, 136470, 136471, 136473, 136474, 136475, 136476, 136477, 136478, 136479, 136483, 136484, 136486
-
-
?
glucocerebroside + H2O
glucose + ceramide
-
-
-
-
?
glucosylceramide + H2O
beta-D-glucose + ceramide
-
-
-
-
?
glucosylceramide + H2O
beta-D-glucose + ceramide
-
-
-
?
glucosylceramide + H2O
beta-D-glucose + ceramide
-
-
-
?
laminaritriose + H2O
?
59% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
laminaritriose + H2O
?
59% activity compared to 4-nitrophenyl beta-D-glucoside
-
-
?
nonylumbelliferyl-beta-D-glucopyranoside + H2O
nonylumbelliferone + glucose
-
-
-
-
?
nonylumbelliferyl-beta-D-glucopyranoside + H2O
nonylumbelliferone + glucose
-
-
-
-
?
nonylumbelliferyl-beta-D-glucopyranoside + H2O