Literature summary for 3.2.1.41 extracted from

Wang, Q.Y.; Xie, N.Z.; Du, Q.S.; Qin, Y.; Li, J.X.; Meng, J.Z.; Huang, R.B.
Active hydrogen bond network (AHBN) and applications for improvement of thermal stability and pH-sensitivity of pullulanase from Bacillus naganoensis (2017), PLoS ONE, 12, e0169080 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21(DE3)	Pullulanibacillus naganoensis

Protein Variants

Protein Variants	Comment	Organism
additional information	the active hydrogen bond network is applied for engineering of pullulanase and improvement of thermal stability and pH-sensitivity of the enzyme	Pullulanibacillus naganoensis
N680D	mutation shows positive effects for the hydrolysis reaction of pullulanase	Pullulanibacillus naganoensis
N680D/T477N	mutation improves thermal stability, pH-sensitivity, and catalysis activity of pullulanase	Pullulanibacillus naganoensis
T477N	mutation shows positive effects for the hydrolysis reaction of pullulanase	Pullulanibacillus naganoensis

Organism

Organism	UniProt	Comment	Textmining
Pullulanibacillus naganoensis	G9JLV4	-	-
Pullulanibacillus naganoensis ATCC 53909	G9JLV4	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pullulanibacillus naganoensis

Synonyms

Synonyms	Comment	Organism
BNPulA324	-	Pullulanibacillus naganoensis

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Release 2023.1 (January 2023)