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1,4-alpha-D-glucan glucanohydrolase
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Alpha-dextrin endo-1,6-alpha-glucosidase
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amylopectin 6-glucanohydrolase
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EC 3.2.1.69
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formerly
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glucanohydrolase, amylopectin 6-
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Nostoc punctiforme debranching enzyme
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PelBsp-PulA
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a nonacylated variant of PulA made by replacing the lipoprotein signal peptide with the signal peptide of pectate lyase PelB from Erwinia chrysanthemi
Pullulan 6-glucanohydrolase
pullulan-6-glucanohydrolase
amylase-pullulanase

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amylopullulanase

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AmyX

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Apu

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ApuK885

truncated enzyme form
ApuK885
truncated enzyme form
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ApuM957

truncated enzyme form
ApuM957
truncated enzyme form
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Ask

EPZ37738
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BmPul

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BNPulA324

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debranching enzyme

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debranching enzyme
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similar enzyme
debranching enzyme
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similar enzyme
Pcal-1616

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PUL US105

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Pul3YH5

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PulASK

EPZ37738
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pulGT

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Pullulan 6-glucanohydrolase

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Pullulan 6-glucanohydrolase
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pullulan-6-glucanohydrolase

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pullulan-6-glucanohydrolase
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pullulanase

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pullulanase type 1

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pullulanase type I

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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan
pullulanase type I
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan
pullulanase type I
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan
pullulanase type I
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan
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pullulanase type I
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan
pullulanase type I
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan
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pullulanase type I
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan
pullulanase type I
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan
pullulanase type I
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan
pullulanase type II

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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
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pullulanase type II
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
pullulanase type II
Thermoanaerobium sp.
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hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides
Saci_1162

locus name
TPApu

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type II pullulanase

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additional information

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the enzyme belongs to a family of 13 glycosyl hydrolases, also called the alpha-amylase family
additional information
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the enzyme belongs to the glycosidase family GH13
additional information
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pullulanase secreton components PulM and PulL
additional information
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the enzyme belongs to the glycosyl hydrolase 13 family, GH13
Promozyme 200 L
additional information
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a heat-stable pullulanase, pullulan 6-glucanohydrolase
pullulanase
additional information
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pullulanase type 1
additional information
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3 glycogen + H2O
4 maltotriose
4-nitrophenyl alpha-D-maltoheptaoside + H2O
4-nitrophenol + alpha-D-maltoheptaose
4-nitrophenyl alpha-D-maltotrioside + H2O
4-nitrophenol + alpha-D-maltotriose
42-O-beta-D-galactosyl-maltose + beta-cyclodextrin
(Gal-G2)-beta-cyclodextrin + (Gal-G2)2-beta-cyclodextrin
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analysis of reaction conditions and structural identification of products
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r
6-O-alpha-maltosyl cyclodextrin + H2O
glucose + cyclodextrin
6-O-alpha-maltotriosyl cyclomaltoheptaose + H2O
glucose + cyclodextrin
6-O-alpha-maltotriosyl cyclomaltohexaose + H2O
glucose + cyclodextrin
alpha-cyclodextrin + H2O
?
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-
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?
amylopectin + H2O
D-glucose + maltose + maltotriose
amylopectin + H2O
malto-2-4-oligosaccharide + ?
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-
-
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?
amylopectin + H2O
maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
amylopectin + H2O
maltooligosaccharides + maltose
amylopectin + H2O
maltotriose
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?
amylopectin + H2O
maltotriose + maltose + ?
amylopectin + H2O
maltotriose + maltose + maltooligosaccharides
amylopectin + H2O
maltotriose + maltotetraose + maltopentaose + maltohexaose
amylopectin beta-limit dextrin + H2O
maltotriose + maltose
amylose + H2O
malto-2-4-oligosaccharide + ?
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?
amylose + H2O
maltooligosaccharides + maltose
amylose + H2O
maltose + maltotriose
amylose + H2O
maltotriose + maltose
beta-cyclodextrin + H2O
?
beta-limit dextrin + H2O
?
beta-limited dextrin + H2O
maltotriose + maltose + maltooligosaccharides
cyclomaltooctaose + H2O
6,6-di-O-alpha-maltosyl-cG8
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gamma-cyclodextrin
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?
dextran + H2O
?
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?
dextrin + H2O
maltooligosaccharides + maltose
gamma-cyclodextrin + H2O
?
glycogen + H2O
malto-2-4-oligosaccharide + ?
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?
glycogen + H2O
maltooligosaccharides + maltose
glycogen + H2O
maltotriose + maltose
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?
glycogen + H2O
maltotriose + maltose + maltooligosaccharides
glycogen beta-limit dextrin + H2O
maltotriose + maltose + maltosetetraose
guar galactomannan + H2O
?
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?
maltoheptaose + H2O
maltotriose + maltose + D-glucose
maltohexaose + H2O
maltotriose + maltose + D-glucose + maltotetraose
maltopentaose + H2O
maltotriose + maltose
maltose + cG8
6-O-alpha-maltosyl-cG8
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through the reversed action
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r
maltotetraose + H2O
maltose + glucose + maltotriose
phytoglycogen + H2O
?
9% of activity compared to pullulan
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?
potato starch + H2O
?
14% of the activity with pullulan
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?
pullulan + H2O
malto-2-4-oligosaccharide + ?
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best substrate
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?
pullulan + H2O
maltotriose + ?
pullulan + H2O
maltotriose + maltose
pullulan + H2O
maltotriose + maltose + D-glucose
this enzyme can attack alpha-1,6- and alpha-1,4-glycosidic linkages in pullulan, and produce a mixture of maltotriose, maltose and glucose
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?
rice flour + H2O
?
15% of the activity with pullulan
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?
soluble starch
maltotriose + maltose
soluble starch + H2O
malto-2-4-oligosaccharide + ?
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?
soluble starch + H2O
maltotriose + maltose
soluble starch + H2O
maltotriose + maltose + maltooligosaccharides
12.8% of the activity with pullulan
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?
starch + H2O
D-glucose + maltose + maltotriose
starch + H2O
maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
starch + H2O
maltose + maltotriose
starch + H2O
maltotriose + ?
starch + H2O
maltotriose + oligosaccharides
metroxylon sagu + H2O
debranched starch
3 glycogen + H2O

4 maltotriose
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?
3 glycogen + H2O
4 maltotriose
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?
4-nitrophenyl alpha-D-maltoheptaoside + H2O

4-nitrophenol + alpha-D-maltoheptaose
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hydrolyzed in an exo-fashion
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?
4-nitrophenyl alpha-D-maltoheptaoside + H2O
4-nitrophenol + alpha-D-maltoheptaose
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hydrolyzed in an exo-fashion
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?
4-nitrophenyl alpha-D-maltoheptaoside + H2O
4-nitrophenol + alpha-D-maltoheptaose
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hydrolyzed in an exo-fashion
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?
4-nitrophenyl alpha-D-maltotrioside + H2O

4-nitrophenol + alpha-D-maltotriose
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hydrolyzed in an exo-fashion
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?
4-nitrophenyl alpha-D-maltotrioside + H2O
4-nitrophenol + alpha-D-maltotriose
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hydrolyzed in an exo-fashion
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?
6-O-alpha-maltosyl cyclodextrin + H2O

glucose + cyclodextrin
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similar enzyme
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-
?
6-O-alpha-maltosyl cyclodextrin + H2O
glucose + cyclodextrin
-
similar enzyme
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-
?
6-O-alpha-maltotriosyl cyclomaltoheptaose + H2O

glucose + cyclodextrin
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-
-
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?
6-O-alpha-maltotriosyl cyclomaltoheptaose + H2O
glucose + cyclodextrin
-
similar enzyme
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-
?
6-O-alpha-maltotriosyl cyclomaltohexaose + H2O

glucose + cyclodextrin
-
similar enzyme
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-
?
6-O-alpha-maltotriosyl cyclomaltohexaose + H2O
glucose + cyclodextrin
-
similar enzyme
-
-
?
amylopectin + H2O

?
35% of activity compared to pullulan
-
-
?
amylopectin + H2O
?
the enzyme specifically attacks alpha-1,6-linkages of branched oligosaccharides
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-
?
amylopectin + H2O
?
the enzyme specifically attacks alpha-1,6-linkages of branched oligosaccharides
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-
?
amylopectin + H2O
?
12% of the activity compared to pullulan
-
-
?
amylopectin + H2O
?
-
-
-
?
amylopectin + H2O
?
the enzyme prefers to debranch the DP6-12 side chains of amylopectin at pH 4.5 and 100°C
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-
?
amylopectin + H2O
?
the enzyme prefers to debranch the DP6-12 side chains of amylopectin at pH 4.5 and 100°C
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-
?
amylopectin + H2O
?
-
-
-
?
amylopectin + H2O
?
-
hydrolyzed at 60% compared to pullulan
-
-
?
amylopectin + H2O
?
20% of activity compared to pullulan
-
-
?
amylopectin + H2O

D-glucose + maltose + maltotriose
the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans
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?
amylopectin + H2O
D-glucose + maltose + maltotriose
the enzyme can degrade both the alpha-1,4 and alpha-1,6-linkages of alpha-glucans
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-
?
amylopectin + H2O

maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
-
-
-
?
amylopectin + H2O
maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose + D-glucose
-
-
-
?
amylopectin + H2O

maltooligosaccharides + maltose
-
-
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
branched polysaccharides
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
-
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
branched polysaccharides
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
-
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
branched polysaccharides
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
-
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
branched polysaccharides
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
-
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
branched polysaccharides
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
-
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
branched polysaccharides
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
-
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
branched polysaccharides
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose
-
-
-
-
?
amylopectin + H2O
maltooligosaccharides + maltose