Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 M, 1.1fold activation | unidentified | |
EDTA | 1 M, 1.3fold activation | unidentified |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.1 M, 50% loss of activity | unidentified | |
Hg2+ | 0.1 M, 70% loss of activity | unidentified | |
iodoacetate | 1 M, 10% loss of activity | unidentified | |
Sodium fluoride | 1 M, 10% loss of activity | unidentified |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
pullulan | pH 6, 40°C | unidentified |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.1 M, 1.4fold activation | unidentified | |
Fe2+ | 0.1 M, 1.6fold activation | unidentified | |
Mn2+ | 0.1 M, 1.5fold activation | unidentified |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
112000 | - |
SDS-PAGE | unidentified |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
unidentified | - |
edible white mushrooms | - |
Purification (Comment) | Organism |
---|---|
- |
unidentified |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | hydrolyzed at 60% compared to pullulan | unidentified | ? | - |
? | |
glycogen + H2O | hydrolyzed at 30% compared to pullulan | unidentified | ? | - |
? | |
pullulan + H2O | - |
unidentified | maltotriose + ? | - |
? | |
soluble starch + H2O | hydrolyzed at 80% compared to pullulan | unidentified | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 112000, SDS-PAGE | unidentified |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
unidentified |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30°C: about 60% of maximal activity, 70°C: about 40% of maximal activity | unidentified |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, 50% loss of activity | unidentified |
70 | - |
45 min, 80% loss of activity | unidentified |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
unidentified |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 30% of maximal activity, pH 8.0: about 30% of maximal activity | unidentified |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
unidentified | isoelectric focusing | - |
6.2 |