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Literature summary for 3.2.1.41 extracted from
- Purification and preliminary characterization of an extracellular pullulanase from Thermoanaerobium Tok6-B1 (1987), Appl. Microbiol. Biotechnol., 26, 427-433.
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermoanaerobium sp. | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Thermoanaerobium sp. |  |
| Sr2+ | - |
Thermoanaerobium sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
gel filtration | Thermoanaerobium sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Thermoanaerobium sp. | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Thermoanaerobium sp. | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobium sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoanaerobium sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1352 | 1790 | - |
Thermoanaerobium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Thermoanaerobium sp. | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Thermoanaerobium sp. | maltooligosaccharides + maltose | - |
? | |
| amylose + H2O | - |
Thermoanaerobium sp. | maltooligosaccharides + maltose | - |
? | |
| maltotetraose + H2O | slowly | Thermoanaerobium sp. | maltose + glucose + maltotriose | - |
? | |
| pullulan + H2O | - |
Thermoanaerobium sp. | maltotriose + ? | - |
? | |
| soluble starch | - |
Thermoanaerobium sp. | maltotriose + maltose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Thermoanaerobium sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
instable above | Thermoanaerobium sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4830 | - |
pullulan | - |
Thermoanaerobium sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Thermoanaerobium sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | - |
Thermoanaerobium sp. |
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