Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.40 extracted from

  • Li, B.; Ji, Y.; Li, Y.; Ding, G.
    Characterization of a glycoside hydrolase family 78 alpha-L-rhamnosidase from Bacteroides the taiotaomicron VPI-5482 and identification of functional residues (2018), 3 Biotech, 8, 120 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.40

Application

Application Comment Organism
industry the enzyme is a promising alternative biocatalyst for industrial applications due to good pH stability, relatively high thermostability and tolerance to low concentration of alcohols Bacteroides thetaiotaomicron

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3) Bacteroides thetaiotaomicron

Protein Variants

Protein Variants Comment Organism
D330A 2350fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron
D335A 560fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron
D335E 2770fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron
D335N 1170fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron
D342A 7670fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron
E595A 6970fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron
E595Q 19170fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron
H620A 40fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron
R334A 280fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron
W339A 1230fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron
W440A 530fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron
Y383A 740fold reduction in specific activity as compared to the wild-type enzyme Bacteroides thetaiotaomicron

Inhibitors

Inhibitors Comment Organism Structure
acetone 1% (v/v), about 50% loss of activity, 5% (v/v), about 75% loss of activity Bacteroides thetaiotaomicron
acetonitrile 1% (v/v), about 55% loss of activity, 5% (v/v), about 90% loss of activity Bacteroides thetaiotaomicron
DMSO 1% (v/v), about 45% loss of activity, 5% (v/v), about 75% loss of activity Bacteroides thetaiotaomicron
ethanol 1% (v/v), about 25% loss of activity, 5% (v/v), about 55% loss of activity Bacteroides thetaiotaomicron
ethylene glycol 1% (v/v), about 15% loss of activity, 5% (v/v), about 30% loss of activity Bacteroides thetaiotaomicron
Isopropanol 1% (v/v), about 25% loss of activity, 5% (v/v), about 55% loss of activity Bacteroides thetaiotaomicron
methanol 1% (v/v), about 15% loss of activity, 5% (v/v), about 50% loss of activity Bacteroides thetaiotaomicron
N,N-Dimethylformamide 1% (v/v), about 70% loss of activity, 5% (v/v), about 90% loss of activity Bacteroides thetaiotaomicron

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
 4-nitrophenyl alpha-L-rhamnopyranoside 50°C, pH 6.5 Bacteroides thetaiotaomicron

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 SDS-PAGE Bacteroides thetaiotaomicron

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron Q8A916
-
-
Bacteroides thetaiotaomicron VPI-5482 Q8A916
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Bacteroides thetaiotaomicron 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?

Subunits

Subunits Comment Organism
? x * 86000, SDS-PAGE Bacteroides thetaiotaomicron

Synonyms

Synonyms Comment Organism
BtRha78A
-
 Bacteroides thetaiotaomicron

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacteroides thetaiotaomicron

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 65 35°C: about 50% of maximal activity, 65°C: about 70% of maximal activity Bacteroides thetaiotaomicron

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life: approximately 100 min Bacteroides thetaiotaomicron

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
69.8
-
 4-nitrophenyl alpha-L-rhamnopyranoside 50°C, pH 6.5 Bacteroides thetaiotaomicron

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Bacteroides thetaiotaomicron

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.6 pH 5.5: about 80% of maximal activity, high activity (more than 60%) at alkaline pH 7.5-8.6 Bacteroides thetaiotaomicron

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 1 h, 40 min, enzyme retains 75% of its initial activity Bacteroides thetaiotaomicron
5.5 9 1 h, 40°C, more than 95% of the activity is retained Bacteroides thetaiotaomicron
8.6 9 the activity of BtRha78A is enhanced significantly after the incubation in alkaline environment. The enzyme retains 142% and 133% of initial activity after the incubation of 1 h at 40 °C in 50 mM glycine-NaOH buffer (pH 8.6 and pH 9.0), respectively Bacteroides thetaiotaomicron

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
130
-
 4-nitrophenyl alpha-L-rhamnopyranoside 50°C, pH 6.5 Bacteroides thetaiotaomicron