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2-phenylethyl beta-D-rutinoside + H2O
2-phenylethyl beta-D-glucopyranoside + alpha-L-rhamnose
-
-
-
-
?
20(S)-ginsenoside Rg2 + H2O
20(S)-ginsenoside Rh1 + alpha-L-rhamnose
-
low activity cleaving the alpha-1,2-linkage
-
-
?
3-O-alpha-L-rhamnopyranosyl-alpha-D-mannopyranoside + H2O
alpha-L-rhamnose + alpha-D-mannose
-
-
-
-
?
4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnose
4-nitrophenyl alpha-L-rhamnoside + H2O
alpha-L-rhamnose + 4-nitrophenol
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
2.1% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside
-
?
4-nitrophenyl-alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
4-nitrophenyl-alpha-L-rhamnopyranoside + H2O
alpha-L-rhamnose + 4-nitrophenol
-
-
-
?
6-O-alpha-L-rhamnosyl-D-glucopyranose + H2O
alpha-L-rhamnose + D-glucose
-
rutinose
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
convallotoxin + H2O
strophantidin + alpha-L-rhamnose
-
-
-
-
?
dulcoside A + H2O
rubusoside + alpha-L-rhamnose
no hydrolytic activity against dulcoside A analogues. No transrhamnosylation activity is observed
-
-
?
epimedin C + H2O
icariin + alpha-L-rhamnopyranose
frangulin + H2O
frangula emodin + alpha-L-rhamnose
-
-
-
-
?
gellan + H2O
alpha-L-rhamnose + ?
gellan + H2O
L-rhamnose + ?
geranyl-beta-D-rutinoside + H2O
geranyl-beta-D-glycoside + alpha-L-rhamnose
-
-
-
-
?
ginsenoside Rg2 + H2O
ginsenoside Rh1 + alpha-L-rhamnose
gypenoside-5 + H2O
ginsenoside Rd + alpha-L-rhamnose
hesperidin + H2O
?
-
34% of activity compared to naringin
-
-
?
hesperidin + H2O
? + alpha-L-rhamnose
cleavage of the alpha-1,6-linkage
-
-
?
hesperidin + H2O
hesperetin 7-glucoside + alpha-L-rhamnose
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + L-rhamnose
-
-
-
-
?
hesperidin + H2O
hesperetin 7-O-glucoside + alpha-L-rhamnose
hesperidin + H2O
L-rhamnose + ?
-
-
-
?
high-methoxyl pectin + H2O
pyranoside + alpha-L-rhamnose
-
-
-
-
?
methyl 3-O-alpha-L-rhamnopyranosyl-alpha-D-mannopyranoside + H2O
alpha-L-rhamnose + methyl alpha-D-mannose
-
-
-
-
?
methyl 3-O-alpha-L-rhamnopyranosyl-alpha-D-xylopyranoside + H2O
alpha-L-rhamnose + methyl alpha-D-xylose
-
-
-
-
?
methyl 3-O-alpha-L-rhamnopyranosyl-alpha-L-rhamnopyranoside + H2O
alpha-L-rhamnose + methyl alpha-L-rhamnose
-
-
-
-
?
methyl 4-O-alpha-L-rhamnopyranosyl-alpha-D-galactopyranoside + H2O
alpha-L-rhamnose + methyl alpha-D-galactose
-
-
-
-
?
methyl 4-O-alpha-L-rhamnopyranosyl-alpha-D-mannopyranoside + H2O
alpha-L-rhamnose + methyl alpha-D-mannose
-
-
-
-
?
methyl 4-O-alpha-L-rhamnopyranosyl-alpha-D-xylopyranoside + H2O
alpha-L-rhamnose + alpha-D-xylose
-
-
-
-
?
muscat glycoside extract
linalool + alpha-terpineol + citronellol + nerol + geraniol + ?
-
-
-
-
?
myricetrin + H2O
3,5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-chromen-4-one + alpha-L-rhamnose
22.5% of the activity compared to naringin
-
-
?
myricitrin + H2O
3,5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-chromen-4-one + alpha-L-rhamnose
low activity
-
-
?
myricitrin + H2O
L-rhamnose + myricetin
-
myricitrin is identical with myricetin-3-L-rhamnoside
-
?
naringin + H2O
4',5,7-trihydroxyflavanone 7-O-beta-D-glucoside + L-rhamnose
-
-
-
-
?
naringin + H2O
4',5,7-trihydroxyflavanone-7-beta-D-glucoside + alpha-L-rhamnose
naringin + H2O
4',5,7-trihydroxyflavanone-7-beta-D-glucoside + L-rhamnose
substrate naringin is preferred over hesperidin
-
-
?
naringin + H2O
? + alpha-L-rhamnose
preferred substrate, cleavage of the alpha-1,2-linkage
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
naringin + H2O
L-rhamnose + ?
naringin dihydrochalcone + H2O
trilobatin + alpha-L-rhamnose
neohesperidin + H2O
5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl beta-D-glucopyranoside + alpha-L-rhamnose
neohesperidin dihydrochalcone + H2O
3,5-dihydroxy-4-[3-(3-hydroxy-4-methoxyphenyl)propanoyl]phenyl beta-D-glucopyranoside + alpha-L-rhamnose
-
-
-
?
p-nitrophenol-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
p-nitrophenol + alpha-L-arabinofuranose
-
-
6.1% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
p-nitrophenyl-beta-D-fucopyranoside + H2O
p-nitrophenol + beta-D-fucopyranose
p-nitrophenyl-beta-D-galactopyranoside + H2O
p-nitrophenol + beta-D-galactopyranose
-
1.6% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside
-
?
periplor alpha-L-rhamnoside + H2O
periplogenin + alpha-L-rhamnose
-
-
-
-
?
polygalacturonic acid + H2O
D-galacturonic acid
-
-
-
-
?
poncirin + H2O
poncirein + alpha-L-rhamnose
Bacteroides JY-6
-
-
-
-
?
proscillaridin A + H2O
pyranosyl 3,14-dihydroxybufa-4,20,22-trienolid + alpha-L-rhamnose
-
-
-
-
?
quercetin 3-alpha-L-rhamnopyranoside + H2O
quercetin + L-rhamnose
-
31% of activity compared to naringin
-
-
?
quercitrin + H2O
quercetin + alpha-L-rhamnose
quercitrin + H2O
quercetin + L-rhamnose
rhamnogalacturonan tetramer + H2O
?
-
-
-
?
rhamnosyl-glucose + H2O
glucose + alpha-L-rhamnose
robinin + H2O
rhamnose + kaempferol-3-robinoside
-
robinin is identical with kaempferol-3-robinoside-7-rhamnoside
-
?
rutin + H2O
3-glycosylquercetin + alpha-L-rhamnose
rutin + H2O
?
-
63% of activity compared to naringin
-
-
?
rutin + H2O
? + alpha-L-rhamnose
low activity, cleavage of the alpha-1,6-linkage
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
rutin + H2O
L-rhamnose + ?
-
-
-
?
saikosaponin C + H2O
(3beta,16beta)-16-hydroxy-13,28-epoxyolean-11-en-3-yl) 6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside + alpha-L-rhamnose
saikosaponin C + H2O
? + alpha-L-rhamnose
scilliglaucosidin alpha-L-rhamnoside + H2O
scilliglaucosidin + alpha-L-rhamnose
-
-
-
-
?
scilliphaeosidin alpha-L-rhamnoside + H2O
scilliphaeosidin + alpha-L-rhamnose
-
-
-
-
?
additional information
?
-
4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
-
-
-
-
?
4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
-
-
-
-
?
4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
-
rhamnosidase N, naringin
-
-
?
4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
Bacteroides JY-6
-
-
-
-
?
4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
-
-
-
-
?
4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
-
-
-
-
?
4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
-
-
-
-
?
4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
70.1% of the activity compared to naringin
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
70.1% of the activity compared to naringin
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
no activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-xyloside, 4-nitrophenyl beta-D-arabinofuranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
no activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-xyloside, 4-nitrophenyl beta-D-arabinofuranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl beta-D-galactopyranoside
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnose
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnose
-
-
-
?
4-nitrophenyl alpha-L-rhamnoside + H2O
alpha-L-rhamnose + 4-nitrophenol
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnoside + H2O
alpha-L-rhamnose + 4-nitrophenol
-
-
-
?
4-nitrophenyl alpha-L-rhamnoside + H2O
alpha-L-rhamnose + 4-nitrophenol
-
-
-
?
4-nitrophenyl alpha-L-rhamnoside + H2O
alpha-L-rhamnose + 4-nitrophenol
-
-
-
-
?
4-nitrophenyl-alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
4-nitrophenyl-alpha-L-rhamnopyranoside + H2O
4-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
Bacteroides JY-6
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
alpha-L-rhamnoside + H2O
alpha-L-rhamnose + ?
-
alpha-L-rhamnose in glycolipids or glycosides
-
-
?
epimedin C + H2O
icariin + alpha-L-rhamnopyranose
-
-
-
?
epimedin C + H2O
icariin + alpha-L-rhamnopyranose
-
-
-
?
gellan + H2O
alpha-L-rhamnose + ?
hydrolytic reaction, release of rhamnose from the disaccharide
-
-
?
gellan + H2O
alpha-L-rhamnose + ?
release of rhamnose from the disaccharide, hydrolytic reaction
residues interacting with rhamnose that are crucial for enzyme catalysis and/or substrate binding identified by crystallization
-
?
gellan + H2O
alpha-L-rhamnose + ?
release of rhamnose from the substrate, hydrolytic reaction
-
-
?
gellan + H2O
alpha-L-rhamnose + ?
hydrolytic reaction, release of rhamnose from the disaccharide
-
-
?
gellan + H2O
alpha-L-rhamnose + ?
release of rhamnose from the substrate, hydrolytic reaction
-
-
?
gellan + H2O
alpha-L-rhamnose + ?
release of rhamnose from the disaccharide, hydrolytic reaction
residues interacting with rhamnose that are crucial for enzyme catalysis and/or substrate binding identified by crystallization
-
?
gellan + H2O
L-rhamnose + ?
-
-
-
?
gellan + H2O
L-rhamnose + ?
-
-
-
?
ginsenoside Rg2 + H2O
ginsenoside Rh1 + alpha-L-rhamnose
low activity
-
-
?
ginsenoside Rg2 + H2O
ginsenoside Rh1 + alpha-L-rhamnose
low activity
-
-
?
gypenoside-5 + H2O
ginsenoside Rd + alpha-L-rhamnose
-
-
-
-
?
gypenoside-5 + H2O
ginsenoside Rd + alpha-L-rhamnose
-
cleavage of the alpha-1,6-linkage
-
-
?
hesperidin + H2O
hesperetin 7-glucoside + alpha-L-rhamnose
-
-
-
-
?
hesperidin + H2O
hesperetin 7-glucoside + alpha-L-rhamnose
substrate naringin is preferred over hesperidin
-
-
?
hesperidin + H2O
hesperetin 7-glucoside + alpha-L-rhamnose
-
-
-
-
?
hesperidin + H2O
hesperetin 7-glucoside + alpha-L-rhamnose
Bacteroides JY-6
-
-
-
-
?
hesperidin + H2O
hesperetin 7-glucoside + alpha-L-rhamnose
-
-
-
-
?
hesperidin + H2O
hesperetin 7-glucoside + alpha-L-rhamnose
-
-
-
-
?
hesperidin + H2O
hesperetin 7-glucoside + alpha-L-rhamnose
-
-
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
-
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
144.9% of the activity compared to naringin
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
144.9% of the activity compared to naringin
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
134% of the activity compared to 4-nitrophenyl-alpha-L-rhamnopyranoside
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
240.6% of the activity with rutin
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
240.6% of the activity with rutin
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
-
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
-
-
-
?
hesperidin + H2O
hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
-
-
?
hesperidin + H2O
hesperetin 7-O-glucoside + alpha-L-rhamnose
11.2% relative activity compared to rutin
-
-
?
hesperidin + H2O
hesperetin 7-O-glucoside + alpha-L-rhamnose
isoform RhaB2 exhibits highest activity against hesperidin
-
-
?
hesperidin + H2O
hesperetin 7-O-glucoside + alpha-L-rhamnose
isoform RhaB2 exhibits highest activity against hesperidin
-
-
?
hesperidin + H2O
hesperetin 7-O-glucoside + alpha-L-rhamnose
11.2% relative activity compared to rutin
-
-
?
naringin + H2O
4',5,7-trihydroxyflavanone-7-beta-D-glucoside + alpha-L-rhamnose
bitter flavour source in fruit
-
-
?
naringin + H2O
4',5,7-trihydroxyflavanone-7-beta-D-glucoside + alpha-L-rhamnose
bitter flavour source in fruit
-
-
?
naringin + H2O
4',5,7-trihydroxyflavanone-7-beta-D-glucoside + alpha-L-rhamnose
bitter flavour source in fruit, design of a bioreactor producing alpha-L-rhamnose
-
-
?
naringin + H2O
?
-
-
-
-
?
naringin + H2O
?
citrus peel naringin
-
-
?
naringin + H2O
?
the free and immobilized enzymes achieve 76% and 67% hydrolysis of the naringin in Kinnow juice, respectively
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
21% of the activity with rutin
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
26% of the activity compared to 4-nitrophenyl-alpha-L-rhamnopyranoside
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
21% of the activity with rutin
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
the enzyme shows a strong ability to hydrolyze naringin but scarcely acts on other substrates. It shows negligible activities on rutin, hesperidin, quercitrin, ginsenoside Rg2, myricitrin, saikosaponin C and 4-nitrophenyl-alpha-L-rhamnoside
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
the enzyme shows a strong ability to hydrolyze naringin but scarcely acts on other substrates. It shows negligible activities on rutin, hesperidin, quercitrin, ginsenoside Rg2, myricitrin, saikosaponin C and 4-nitrophenyl-alpha-L-rhamnoside
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
Bacteroides JY-6
-
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
-
?
naringin + H2O
beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
-
-
-
?
naringin + H2O
L-rhamnose + ?
-
-
-
?
naringin + H2O
L-rhamnose + ?
-
-
-
?
naringin + H2O
L-rhamnose + ?
-
-
-
?
naringin dihydrochalcone + H2O
trilobatin + alpha-L-rhamnose
-
-
-
?
naringin dihydrochalcone + H2O
trilobatin + alpha-L-rhamnose
-
-
-
?
neohesperidin + H2O
5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl beta-D-glucopyranoside + alpha-L-rhamnose
-
-
-
-
?
neohesperidin + H2O
5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl beta-D-glucopyranoside + alpha-L-rhamnose
-
-
-
-
?
neohesperidin + H2O
5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl beta-D-glucopyranoside + alpha-L-rhamnose
Bacteroides JY-6
-
-
-
-
?
p-nitrophenol-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
assay reaction system to determine enzyme activity in psychrophile bacteria, at 25°C and at 5°C
-
-
?
p-nitrophenol-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
assay reaction system to determine enzyme activity in psychrophile bacteria
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
single displacement reaction mechanism
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
Bacteroides JY-6
-
-
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
p-nitrophenyl alpha-L-rhamnoside + H2O
p-nitrophenol + alpha-L-rhamnose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
assay reaction system to determine activity of wild-type and mutant enzymes
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
assay reaction system to determine activity of wild-type and mutant enzymes
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
Bacteroides JY-6
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
assay reaction system to determine enzyme activity in small and large experiments for bioreactor efficiency
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
p-nitrophenol + alpha-L-rhamnopyranose
-
-
-
-
?
p-nitrophenyl-beta-D-fucopyranoside + H2O
p-nitrophenol + beta-D-fucopyranose
-
2.9% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside
-
?
p-nitrophenyl-beta-D-fucopyranoside + H2O
p-nitrophenol + beta-D-fucopyranose
-
2.9% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside
-
?
proscillaridin A + H2O
?
0.4% relative activity compared to hesperidin
-
-
?
proscillaridin A + H2O
?
4.5% relative activity compared to rutin
-
-
?
quercitrin + H2O
quercetin + alpha-L-rhamnose
-
-
-
-
?
quercitrin + H2O
quercetin + alpha-L-rhamnose
-
-
-
?
quercitrin + H2O
quercetin + L-rhamnose
-
-
-
-
?
quercitrin + H2O
quercetin + L-rhamnose
-
quercitrin is identical with quercetin-3-L-rhamnoside
-
?
quercitrin + H2O
quercetin + L-rhamnose
-
quercitrin is identical with quercetin 3-L-rhamnoside, growth on quercitrin as carbon source
-
?
quercitrin + H2O
quercetin + L-rhamnose
-
-
-
-
?
quercitrin + H2O
quercetin + L-rhamnose
-
-
-
-
?
quercitrin + H2O
quercetin + L-rhamnose
-
-
-
-
?
quercitrin + H2O
quercetin + L-rhamnose
-
-
-
-
?
quercitrin + H2O
quercetin + L-rhamnose
-
-
-
-
?
quercitrin + H2O
quercetin + L-rhamnose
-
rhamnosidase T
-
-
?
rhamnosyl-glucose + H2O
glucose + alpha-L-rhamnose
-
gellan as carbon-source
-
-
?
rhamnosyl-glucose + H2O
glucose + alpha-L-rhamnose
-
gellan as carbon-source
-
-
?
rutin + H2O
3-glycosylquercetin + alpha-L-rhamnose
-
-
-
-
?
rutin + H2O
3-glycosylquercetin + alpha-L-rhamnose
-
rhamnosidase N
-
-
?
rutin + H2O
3-glycosylquercetin + alpha-L-rhamnose
Bacteroides JY-6
-
-
-
-
?
rutin + H2O
3-glycosylquercetin + alpha-L-rhamnose
-
-
-
-
?
rutin + H2O
3-glycosylquercetin + alpha-L-rhamnose
-
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
136.7% of the activity compared to naringin
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
136.7% of the activity compared to naringin
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
68% of the activity compared to 4-nitrophenyl-alpha-L-rhamnopyranoside
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
the yield of isoquercitrin for the wild-type enzyme reached the maximum at 4 h. However, the yield of isoquercitrin for the mutants D594Q and G827K/D594Q enzyme reached the maximum at 6 h. The D594Q and G827K/D594Q mutant enzymes produce 13.5% and 11.0% more isoquercitrin than the wild-type, respectively
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
10.1% relative activity compared to hesperidin
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
isoform RhaB1 exhibits highest activity against rutin
-
-
?
rutin + H2O
isoquercitrin + alpha-L-rhamnose
-
-
-
?
saikosaponin C + H2O
(3beta,16beta)-16-hydroxy-13,28-epoxyolean-11-en-3-yl) 6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside + alpha-L-rhamnose
low activity
-
-
?
saikosaponin C + H2O
(3beta,16beta)-16-hydroxy-13,28-epoxyolean-11-en-3-yl) 6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside + alpha-L-rhamnose
9.19% of the activity compared to naringin
-
-
?
saikosaponin C + H2O
? + alpha-L-rhamnose
Bacteroides JY-6
-
-
-
-
?
saikosaponin C + H2O
? + alpha-L-rhamnose
-
-
-
-
?
additional information
?
-
-
enzyme is able to hydrolyze alpha-1,2- and alpha-1,6-linkages to beta-D-glucosides in addition to direct linkages from the L-rhamnose residue to the aglycon
-
-
?
additional information
?
-
-
the enzyme can not hydrolyze ginsenoside Rg2 or saikosaponin C
-
-
?
additional information
?
-
-
the enzyme can not hydrolyze ginsenoside Rg2 or saikosaponin C
-
-
?
additional information
?
-
no substrate: quercitrin
-
-
?
additional information
?
-
no substrate: quercitrin
-
-
?
additional information
?
-
-
no substrate: quercitrin
-
-
?
additional information
?
-
-
RhaA and RhaB selectively remove terminal alpha-L-rhamnosyl groups in situ in orange juice, blackcurrant juice, and green tea infusion resulting in a decrease of flavonoid rutinosides, i.e. anthocyanins, flavanones, and flavonols, respectively, and concomitant increase in their flavonoid glucoside counterparts, product identification and quantification by HPLC-MS, overview
-
-
?
additional information
?
-
-
RhaA and RhaB selectively remove terminal alpha-L-rhamnosyl groups in situ in orange juice, blackcurrant juice, and green tea infusion resulting in a decrease of flavonoid rutinosides, i.e. anthocyanins, flavanones, and flavonols, respectively, and concomitant increase in their flavonoid glucoside counterparts, product identification and quantification by HPLC-MS, overview
-
-
?
additional information
?
-
enzyme is able to hydrolyze alpha-(1-2) amd alpha-(1-6) glycosidic bonds
-
-
?
additional information
?
-
-
acts both on alpha-1,2- and on alpha-1,6-linkages
-
-
?
additional information
?
-
the enzyme posseses broad substrate specificities by hydrolyzing alpha-1,2, alpha-1,3 alpha-1,4, and alpha-1,6 linkages to beta-D-glucosides
-
-
?
additional information
?
-
the enzyme posseses broad substrate specificities by hydrolyzing alpha-1,2, alpha-1,3 alpha-1,4, and alpha-1,6 linkages to beta-D-glucosides
-
-
?
additional information
?
-
-
no activity with myricetrin
-
-
?
additional information
?
-
-
no activity with myricetrin
-
-
?
additional information
?
-
isozyme plays a crucial role in complete metabolism of gellan
-
-
?
additional information
?
-
isozyme plays a crucial role in complete metabolism of gellan
-
-
?
additional information
?
-
-
isozyme plays a crucial role in complete metabolism of gellan
-
-
?
additional information
?
-
isozyme plays a crucial role in complete metabolism of gellan
-
-
?
additional information
?
-
isozyme plays a crucial role in complete metabolism of gellan
-
-
?
additional information
?
-
no the enzyme exhibits high selectivity to cleave the alpha-1,2 and alpha-1,6 glycosidic bond between rhamnoside and rhamnoside, rhamnoside and glycoside
-
-
?
additional information
?
-
-
no the enzyme exhibits high selectivity to cleave the alpha-1,2 and alpha-1,6 glycosidic bond between rhamnoside and rhamnoside, rhamnoside and glycoside
-
-
?
additional information
?
-
no the enzyme exhibits high selectivity to cleave the alpha-1,2 and alpha-1,6 glycosidic bond between rhamnoside and rhamnoside, rhamnoside and glycoside
-
-
?
additional information
?
-
-
no the enzyme exhibits high selectivity to cleave the alpha-1,2 and alpha-1,6 glycosidic bond between rhamnoside and rhamnoside, rhamnoside and glycoside
-
-
?
additional information
?
-
no activity is detected towards the alpha-1,2 linkage in naringin
-
-
?
additional information
?
-
no activity is detected towards the alpha-1,2 linkage in naringin
-
-
?
additional information
?
-
-
no activity is detected towards the alpha-1,2 linkage in naringin
-
-
?
additional information
?
-
no activity towards quercitin, gellan gum, vitexin 2-O-rhamnoside, and alpha-chaconine
-
-
?
additional information
?
-
no activity towards quercitin, gellan gum, vitexin 2-O-rhamnoside, and alpha-chaconine
-
-
?
additional information
?
-
-
no activity towards quercitin, gellan gum, vitexin 2-O-rhamnoside, and alpha-chaconine
-
-
?
additional information
?
-
no activity is detected towards the alpha-1,2 linkage in naringin
-
-
?
additional information
?
-
no activity is detected towards the alpha-1,2 linkage in naringin
-
-
?
additional information
?
-
no activity towards quercitin, gellan gum, vitexin 2-O-rhamnoside, and alpha-chaconine
-
-
?
additional information
?
-
no activity towards quercitin, gellan gum, vitexin 2-O-rhamnoside, and alpha-chaconine
-
-
?
additional information
?
-
the enzyme is able to hydrolyze rhamnose from natural flavonoids. It hydrolyzes both alpha-1,2 and alpha-1,6 glycosidic linkages
-
-
?
additional information
?
-
no activity with quercitrin
-
-
?
additional information
?
-
no activity with quercitrin
-
-
?
additional information
?
-
no activity with rutin and quercitrin
-
-
?
additional information
?
-
no activity with rutin and quercitrin
-
-
?
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(1R,2S,6R,8S,8aS)-6-methyloctahydroindolizine-1,2,8-triol
-
-
(2S,3R,4S)-benzyl-3,4-dihydroxy pyrrolidine
-
IC50 is 0.168 mM
(2S,3R,4S)-deacetyl anisomycin
-
IC50 is 0.137 mM
(2S,3S,4R)-benzyl-3,4-dihydroxy pyrrolidine
-
competitive
(2S,3S,4R)-deacetyl anisomycin
-
competitive
(2S,3S,4S)-2-methyl-3,4-dihydro-2H-pyrrole-3,4-diol
-
-
(2S,3S,4S)-2-methylpyrrolidine-3,4-diol
-
-
(2S,3S,4S)-anisomycin
-
competitive
(2S,3S,4S)-deacetyl anisomycin
-
competitive
(3S,4R)-3,4-dihydroxy-5-spirocyclopropyl-DELTA-pyrroline
-
-
(3S,4S)-3,4-dihydroxy-5-spirocyclopropyl-DELTA-pyrroline
-
72% inhibition at 1 mM
(6S,7S,8S)-4-azaspiro[2.5]octane-6,7,8-triol
-
72% inhibition at 1 mM
1,10-phenanthroline
less effective inhibitor, when the 1,10-phenanthroline concentration is raised to 10 mM, the activity of isoform RhaB1 decreases by 50%; less effective inhibitor, when the 1,10-phenanthroline concentration is raised to 10 mM, the activity of isoform RhaB2 decreases by 50%
4-Chloromercuriphenylsulfonate
-
strong inhibition
acetonitrile
1% (v/v), about 55% loss of activity, 5% (v/v), about 90% loss of activity
Citric acid
-
no other organic acids, highest inhibition at low pH
dimethyl sulfoxide
the addition of organic solvent can enhance solubility of naringin dihydrochalcone, but it also decreases enzyme activity. Dimethyl sulfoxide, acetone and methanol decrease enzyme activity more seriously than that of ethanol. When necessary, ethanol as a co-solvent with quantity less than 5% (v/v) is ecommended to improve the hydrolysis reaction of naringin dihydrochalcone
DMSO
1% (v/v), about 45% loss of activity, 5% (v/v), about 75% loss of activity
ethylene glycol
1% (v/v), about 15% loss of activity, 5% (v/v), about 30% loss of activity
FeCl3
1 mM, 76% residual activity
five-membered ring azasugars
-
with L-rhamnose configuration, substitution at the nitrogen shifts the inhibition mechanism from mixed to competitive
-
Isopropanol
1% (v/v), about 25% loss of activity, 5% (v/v), about 55% loss of activity
K+
-
10 mM, 5% loss of activity
L-Mannose
Bacteroides JY-6
-
competitive
Li+
-
10 mM, 8% loss of activity
methyl-alpha-D-mannoside
-
competitive
N,N-Dimethylformamide
1% (v/v), about 70% loss of activity, 5% (v/v), about 90% loss of activity
Na+
-
10 mM, 10% loss of activity
p-chlormercuriphenylsulfonic acid
Bacteroides JY-6
-
modification of cysteines
p-chloromercuribenzoate
-
1 mM, 11% residual activity
p-chloromercuribenzoic acid
0.1 mM, 50% residual activity
p-hydroxymercuribenzoate
-
-
phosphoramidon
isoform RhaB1 shows 23.8% residual activity at 1 mM; isoform RhaB2 shows 42.5% residual activity at 1 mM
polyhexamethylene biguanide
1 mM reduces the activity of isoform RhaB1 to 10.9% and isoform RhaB2 to 36.0% residual activity; 1 mM reduces the activity of isoform RhaB1 to 10.9% residual activity
Sodium acetate
-
above 0.3 M , at pH 5
thiorphan
less effective inhibitor; less effective inhibitor
ZnCl2
1 mM, 73% residual activity
[4-([[(2S,3S,4R)-3,4-dihydroxypyrrolidin-2-yl]methyl]amino)-5-hydroxy-6-methylpyridin-2-yl]methyl dihydrogen phosphate
-
-
[4-([[(2S,3S,4S)-3,4-dihydroxypyrrolidin-2-yl]methyl]amino)-5-hydroxy-6-methylpyridin-2-yl]methyl dihydrogen phosphate
-
-
6-deoxy-D-glucose
Bacteroides JY-6
-
competitive
6-deoxy-D-glucose
-
competitive
6-deoxy-D-glucose
-
competitive
acetone
the addition of organic solvent can enhance solubility of naringin dihydrochalcone, but it also decreases enzyme activity. Dimethyl sulfoxide, acetone and methanol decrease enzyme activity more seriously than that of ethanol. When necessary, ethanol as a co-solvent with quantity less than 5% (v/v) is ecommended to improve the hydrolysis reaction of naringin dihydrochalcone
acetone
1% (v/v), about 50% loss of activity, 5% (v/v), about 75% loss of activity
alpha-L-rhamnose
-
competitive
alpha-L-rhamnose
Bacteroides JY-6
-
competitive
alpha-L-rhamnose
-
competitive
Cd2+
-
-
Co2+
-
2 mM, 36% loss of activity
Co2+
-
10 mM, 17% loss of activity
Cu2+
-
activates 1.4fold at 20 mM, inhibits completely at 100 mM
Cu2+
-
2 mM, 53% loss of activity
Cu2+
-
10 mM, 68% loss of activity
Cu2+
Bacteroides JY-6
-
50% inhibition at 2 mM
Cu2+
5 mM, 92% loss of activity. 1 mM, 83% loss of activity
CuCl2
1 mM, 4% residual activity; 1 mM, 70% residual activity
CuCl2
-
1 mM, 11% residual activity
D-glucose
-
21% w/v, decrease in activity by 20%
D-glucose
10 mM, 56% residual activity
D-glucose
-
500 mM, 76% residual activity
D-glucose
-
IC50 value above 0.5 M
D-glucose
about 500 mM D-glucose concentration in grape juice inhibits RhmA by 39%; about 500 mM D-glucose concentration in grape juice inhibits RhmB by 38%
ethanol
-
12% v/v, decrease in activity by 20%
ethanol
1% (v/v), about 25% loss of activity, 5% (v/v), about 55% loss of activity
ethanol
-
12% v/v, 20% inhibition
ethanol
-
presence of 12% ethanol reveals a decreased activity of 48%
ethanol
at 12% v/v, 51% inhibition of RhmB; at 12% v/v, 60% inhibition of RhmA
Fe2+
100 mM, complete inactivation
Fe2+
-
10 mM, 54% loss of activity
Fe2+
5 mM, 12% loss of activity
Fe3+
-
activates 1.4fold at 10 mM, inhibits completely at 50 mM
Fe3+
10 mM, 46% inhibition. 100 mM, 91% inhibition
Fe3+
-
10 mM, 97% loss of activity
Fe3+
Bacteroides JY-6
-
-
Fe3+
5 mM, 12% loss of activity
Hg2+
-
10 mM, 99% loss of activity
Hg2+
1 mM, 99% loss of activity
HgCl2
0.1 mM, 0.23% residual activity
HgCl2
-
1 mM, 11% residual activity
L-fucose
Bacteroides JY-6
-
competitive
L-fucose
-
IC50 value above 0.2 M
L-rhamnose
-
compeitive
L-rhamnose
10 mM, 14% residual activity; 10 mM, 37% residual activity
L-rhamnose
crystallized complex with interacting site determined, for kinetic assay different concentrations ranging between 0 and 100 mM of rhamnose used
L-rhamnose
-
strong product inhibition
L-rhamnose
isoform RhaB1 is slightly inhibited by L-rhamnose, showing noncompetitive inhibition
L-rhamnose
complete inhibition, competitive product inhibition; complete inhibition, competitive product inhibition
methanol
the addition of organic solvent can enhance solubility of naringin dihydrochalcone, but it also decreases enzyme activity. Dimethyl sulfoxide, acetone and methanol decrease enzyme activity more seriously than that of ethanol. When necessary, ethanol as a co-solvent with quantity less than 5% (v/v) is recommended to improve the hydrolysis reaction of naringin dihydrochalcone
methanol
1% (v/v), about 15% loss of activity, 5% (v/v), about 50% loss of activity
Mg2+
-
2 mM, slight inhibition
Mg2+
-
2 mM, 63% inhibition
Mn2+
-
2 mM, slight inhibition
Mn2+
-
10 mM, 5% loss of activity
Ni2+
-
2 mM, complete loss of activity
Ni2+
-
10 mM, 41% loss of activity
Ni2+
5 mM, 28% loss of activity
Pb2+
Bacteroides JY-6
-
-
SDS
SDS produces partial inactivation; SDS produces partial inactivation
Zn2+
-
0.2 mM, complete inhibition
Zn2+
-
2 mM, slight inhibition
Zn2+
-
10 mM, 13 % loss of activity
Zn2+
Bacteroides JY-6
-
-
Zn2+
5 mM, 32% loss of activity. 1 mM, 55% loss of activity
Zn2+
30-35% inhibition at 2 mM, reversible by 10 mM EDTA
additional information
-
neither divalent cations such as Ca2+, Mg2+, Mn2+, and Co2+ nor reducing agents such as 2-mercaptoethanol and dithiothreitol show effects on enzyme activtiy
-
additional information
dithiothreitol and beta-mercaptoethanol have no or little effect on activity; dithiothreitol and beta-mercaptoethanol have no or little effect on activity
-
additional information
dithiothreitol and beta-mercaptoethanol have no or little effect on activity; dithiothreitol and beta-mercaptoethanol have no or little effect on activity
-
additional information
-
dithiothreitol and beta-mercaptoethanol have no or little effect on activity; dithiothreitol and beta-mercaptoethanol have no or little effect on activity
-
additional information
-
not inhibitory at 1 mM: EDTA, CaCl2, 2-mercaptoethanol
-
additional information
-
design from aromatic amino acids and inhibitory potency of polyhydroxylated pyrrolidines, stereochemistry, overview
-
additional information
-
not inhibited by deoxyrhamnojirimycin and 5-C-spirocyclopropyl-(5-demethyl-1-deoxy)-L-fuconojirimycin
-
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Kaji, A.; Ichimi, T.
alpha-L-Rhamnosidase activity in culture filtrate of Corticium rolfsii. Enzymatic activity at low pH
Agric. Biol. Chem.
37
431-432
1973
Athelia rolfsii
-
brenda
Kurosawa, Y.; Ikeda, K.; Egami, F.
alpha-L-Rhamnosidases of the liver of Turbo cornutus and Aspergillus niger
J. Biochem.
73
31-37
1973
Aspergillus niger, Turbo cornutus
brenda
Bourbouze, R.; Pratviel-Sosa, F.; Percheron, F.
Rhamnodiastese et alpha-L-rhamnosidase de Fagopyrum esculentum
Phytochemistry
14
1279-1282
1975
Fagopyrum esculentum
-
brenda
Bourbouze, R.; Percheron, F.; Courtois, J.E.
alpha-L-Rhamnosidase from Fagopyrum esculentum: purification and some properties
Eur. J. Biochem.
63
331-337
1976
Fagopyrum esculentum
brenda
Ono, M.
Inhibitory effects of citric acid in enzymatic debittering of citrus fruit juice
J. Ferment. Technol.
58
387-389
1980
Aspergillus niger
-
brenda
Romero, C.; Manjon, A.; Bastida, J.; Iborra, J.L.
A method for assaying the rhamnosidase activity of naringinase
Anal. Biochem.
149
566-571
1985
Penicillium sp.
brenda
Manjon, A.; Bastida, J.; Romero, A.; Jimeno, A.; Iborra, J.L.
Immobilization of nariginase on glycophase-coated porous glass
Biotechnol. Lett.
7
477-482
1985
Penicillium sp.
-
brenda
Kamiya, S.; Esaki, S.; Ito-Tanaka, R.
Synthesis of certain disaccharides containing a alpha- or beta-L-rhamnopyranosidic group and the substrate specificity of alpha-L-rhamnosidase from Aspergillus niger
Agric. Biol. Chem.
49
2351-2358
1985
Aspergillus niger
-
brenda
Turecek, P.; Pittner, F.
Simple enzyme reactors suitable for the byproduct-free preparation of the agyclones of naturally occurring glycosides under mild conditions
Appl. Biochem. Biotechnol.
13
1-13
1986
Penicillium sp.
-
brenda
Bokkenheuser, V.D.; Shackleton, C.H.L.; Winter, J.
Hydrolysis of dietary flavonoid glycosides by strains of intestinal Bacteroides from humans
Biochem. J.
248
953-956
1987
Parabacteroides distasonis
brenda
Jimeno, A.; Manjon, A.; Canovas, M.; Iborra, J.L.
Use of narginase immobilized on glycophase-coated glass for fruit juice debittering
Process Biochem.
22
13-16
1987
Penicillium sp.
-
brenda
Mutter, M.; Beldman, G.; Schols, H.A.; Voragen, A.G.
Rhamnogalacturonan alpha-L-rhamnopyranohydrolase. A novel enzyme specific for the terminal nonreducing rhamnosyl unit in rhamnogalacturonan regions of pectin
Plant Physiol.
106
241-250
1994
Aspergillus aculeatus
brenda
Provencher, L.; Steensma, D.H.; Wong, C.H.
Five-membered ring azasugars as potent inhibitors of alpha-L-rhamnosidase (naringinase) from Penicillium decumbens
Bioorg. Med. Chem.
2
1179-1188
1994
Penicillium sp.
brenda
Jang, I.S.; Kim, D.H.
Purification and characterization of alpha-L-rhamnosidase from Bacteroides JY-6, a human intestinal bacterium
Biol. Pharm. Bull.
19
1546-1549
1996
Bacteroides JY-6
brenda
Manzanares, P.; de Graaff, L.H.; Visser, J.
Purification and characterization of an alpha-L-rhamnosidase from Aspergillus niger
FEMS Microbiol. Lett.
157
279-283
1997
Aspergillus niger
brenda
Pitson, S.M.; Mutter, M.; van den Broek, L.A.; Voragen, A.G.; Beldman, G.
Stereochemical course of hydrolysis catalysed by alpha-L-rhamnosyl and alpha-D-galacturonosyl hydrolases from Aspergillus aculeatus
Biochem. Biophys. Res. Commun.
242
552-559
1998
Aspergillus aculeatus
brenda
Hashimoto, W.; Nankai, H.; Sato, N.; Kawai, S.; Murata, K.
Characterization of alpha-L-rhamnosidase of Bacillus sp. GL1 responsible for the complete depolymerization of gellan
Arch. Biochem. Biophys.
368
56-60
1999
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) GL1
brenda
Zverlov, V.V.; Hertel, C.; Bronnenmeier, K.; Hroch, A.; Kellermann, J.; Schwarz, W.H.
The thermostable alpha-L-rhamnosidase RamA of Clostridium stercorarium: biochemical characterization and primary structure of a bacterial alpha-L-rhamnoside hydrolase, a new type of inverting glycoside hydrolase
Mol. Microbiol.
35
173-179
2000
Thermoclostridium stercorarium
brenda
Miake, F.; Satho, T.; Takesue, H.; Yanagida, F.; Kashige, N.; Watanabe, K.
Purification and characterization of intracellular alpha-L-rhamnosidase from Pseudomonas paucimobilis FP2001
Arch. Microbiol.
173
65-70
2000
Sphingomonas paucimobilis, Sphingomonas paucimobilis FP2001
brenda
Westlake, D.W.S.
Microbial degradation of quercitrin
Can. J. Microbiol.
9
211-220
1963
Aspergillus flavus
-
brenda
Manzanares, P.; van den Broeck, H.C.; de Graaff, L.H.; Visser, J.
Purification and characterization of two different alpha-L-rhamnosidases, RhaA and RhaB, from Aspergillus aculeatus
Appl. Environ. Microbiol.
67
2230-2234
2001
Aspergillus aculeatus (Q9C1M9), Aspergillus aculeatus (Q9HFW5), Aspergillus aculeatus
brenda
Hashimoto, W.; Miyake, O.; Nankai, H.; Murata, K.
Molecular identification of an alpha-L-rhamnosidase from Bacillus sp strain GL1 as an enzyme involved in complete metabolism of gellan
Arch. Biochem. Biophys.
415
235-244
2003
Bacillus sp. (in: Bacteria) (Q93RE7), Bacillus sp. (in: Bacteria) (Q93RE8), Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) GL1 (Q93RE7), Bacillus sp. (in: Bacteria) GL1 (Q93RE8)
brenda
Yanai, T.; Sato, M.
Purification and characterization of an alpha-L-rhamnosidase from Pichia angusta X349
Biosci. Biotechnol. Biochem.
64
2179-2185
2000
Ogataea angusta
brenda
Soria, F.; Ellenrieder, G.
Thermal inactivation and product inhibition of Aspergillus terreus CECT 2663 alpha-L-rhamnosidase and their role on hydrolysis of naringin solutions
Biosci. Biotechnol. Biochem.
66
1442-1449
2002
Aspergillus terreus, Aspergillus terreus CECT 2663
brenda
Gallego, M.V.; Pinaga, F.; Ramon, D.; Valles, S.
Purification and characterization of an alpha-L-rhamnosidase from Aspergillus terreus of interest in winemaking.
J. Food Sci.
66
204-209
2001
Aspergillus terreus
brenda
Manzanares, P.; Orejas, M.; Ibanez, E.; Valles, S.; Ramon, D.
Purification and characterization of an alpha-L-rhamnosidase from Aspergillus nidulans
Lett. Appl. Microbiol.
31
198-202
2000
Aspergillus nidulans
brenda
Scaroni, E.; Cuevas, C.; Carrillo, L.; Ellenrieder, G.
Hydrolytic properties of crude alpha-L-rhamnosidases produced by several wild strains of mesophilic fungi
Lett. Appl. Microbiol.
34
461-465
2002
Aspergillus flavus, Aspergillus luchuensis, Fusarium sambucinum, Fusarium solani, Mucor racemosus, Penicillium aurantiogriseum, Trichoderma longibrachiatum, Fusarium sambucinum 310, Penicillium aurantiogriseum 695, Fusarium solani 706, Aspergillus flavus S1, Mucor racemosus 709, Aspergillus luchuensis IFO 4308, Trichoderma longibrachiatum 708
brenda
Kim, J.H.; Curtis-Long, M.J.; Seo, W.D.; Lee, J.H.; Lee, B.W.; Yoon, Y.J.; Kang, K.Y.; Park, K.H.
alpha-Rhamnosidase inhibitory activities of polyhydroxylated pyrrolidine
Bioorg. Med. Chem. Lett.
15
4282-4285
2005
Penicillium decumbens
brenda
Miyata, T.; Kashige, N.; Satho, T.; Yamaguchi, T.; Aso, Y.; Miake, F.
Cloning, sequence analysis, and expression of the gene encoding Sphingomonas paucimobilis FP2001 alpha-L -rhamnosidase
Curr. Microbiol.
51
105-109
2005
Sphingomonas paucimobilis (Q76LC4)
brenda
Birgisson, H.; Hreggvidsson, G.O.; Fridjonsson, O.H.; Mort, A.; Kristjansson, J.K.; Mattiasson, B.
Two new thermostable alpha-L-rhamnosidases from a novel thermophilic bacterium
Enzyme Microb. Technol.
34
561-571
2004
Thermomicrobia bacterium (Q6RCI8), Thermomicrobia bacterium (Q6RCI9)
-
brenda
Gonzalez-Barrio, R.; Trindade, L.M.; Manzanares, P.; de Graaff, L.H.; Tomas-Barberan, F.A.; Espin, J.C.
Production of bioavailable flavonoid glucosides in fruit juices and green tea by use of fungal alpha-L-rhamnosidases
J. Agric. Food Chem.
52
6136-6142
2004
Aspergillus aculeatus, Aspergillus aculeatus NW240
brenda
Park, S.; Kim, J.; Kim, D.
Purification and characterization of quercitrin-hydrolyzing alpha-L-rhamnosidase from Fusobacterium K-60, a human intestinal bacterium
J. Microbiol. Biotechnol.
15
519-524
2005
Fusobacterium sp., Fusobacterium sp. K-60
-
brenda
Yu, H.; Liu, H.; Zhang, C.; Tan, D.; Lu, M.; Jin, F.
Purification and characterization of gypenoside-alpha-L-rhamnosidase hydrolyzing gypenoside-5 into ginsenoside Rd
Process Biochem.
39
861-867
2004
Absidia sp. (in: Fungi)
-
brenda
Cui, Z.; Maruyama, Y.; Mikami, B.; Hashimoto, W.; Murata, K.
Crystallization and preliminary crystallographic analysis of the family GH78 alpha-L-rhamnosidase RhaB from Bacillus sp. GL1
Acta Crystallogr. Sect. F
62
646-648
2006
Bacillus sp. (in: Bacteria) (Q93RE7), Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) GL1 (Q93RE7)
brenda
Birgisson, H.; Wheat, J.O.; Hreggvidsson, G.O.; Kristjansson, J.K.; Mattiasson, B.
Immobilization of a recombinant Escherichia coli producing a thermostable alpha -L-rhamnosidase: Creation of a bioreactor for hydrolyses of naringin
Enzyme Microb. Technol.
40
1181-1187
2007
Thermomicrobia bacterium (Q6RCI9)
-
brenda
Orrillo, A.G.; Ledesma, P.; Delgado, O.D.; Spagna, G.; Breccia, J.D.
Cold-active alpha -L-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem
Enzyme Microb. Technol.
40
236-241
2007
Ralstonia pickettii, Pseudoalteromonas sp.
-
brenda
Cui, Z.; Maruyama, Y.; Mikami, B.; Hashimoto, W.; Murata, K.
Crystal structure of glycoside hydrolase family 78 alpha-L-Rhamnosidase from Bacillus sp. GL1
J. Mol. Biol.
374
384-398
2007
Bacillus sp. (in: Bacteria) (Q93RE7), Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) GL1 (Q93RE7)
brenda
Magario, I.; Neumann, A.; Oliveros, E.; Syldatk, C.
Deactivation kinetics and response surface analysis of the stability of alpha-L-rhamnosidase from Penicillium decumbens
Appl. Biochem. Biotechnol.
152
29-41
2009
Penicillium decumbens
brenda
Koseki, T.; Mese, Y.; Nishibori, N.; Masaki, K.; Fujii, T.; Handa, T.; Yamane, Y.; Shiono, Y.; Murayama, T.; Iefuji, H.
Characterization of an alpha-L-rhamnosidase from Aspergillus kawachii and its gene
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