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Literature summary for 3.2.1.40 extracted from

  • Avila, M.; Jaquet, M.; Moine, D.; Requena, T.; Pelaez, C.; Arigoni, F.; Jankovic, I.
    Physiological and biochemical characterization of the two alpha-L-rhamnosidases of Lactobacillus plantarum NCC245 (2009), Microbiology, 155, 2739-2749.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA a slight activating influence on isoform RhaB1 is observed for EDTA at 10 mM Lactiplantibacillus plantarum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 S1 cells Lactiplantibacillus plantarum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline less effective inhibitor, when the 1,10-phenanthroline concentration is raised to 10 mM, the activity of isoform RhaB1 decreases by 50%; less effective inhibitor, when the 1,10-phenanthroline concentration is raised to 10 mM, the activity of isoform RhaB2 decreases by 50% Lactiplantibacillus plantarum
Cu2+
-
Lactiplantibacillus plantarum
Fe2+
-
Lactiplantibacillus plantarum
L-rhamnose isoform RhaB1 is slightly inhibited by L-rhamnose, showing noncompetitive inhibition Lactiplantibacillus plantarum
Mn2+
-
Lactiplantibacillus plantarum
additional information dithiothreitol and beta-mercaptoethanol have no or little effect on activity; dithiothreitol and beta-mercaptoethanol have no or little effect on activity Lactiplantibacillus plantarum
phosphoramidon isoform RhaB1 shows 23.8% residual activity at 1 mM; isoform RhaB2 shows 42.5% residual activity at 1 mM Lactiplantibacillus plantarum
polyhexamethylene biguanide 1 mM reduces the activity of isoform RhaB1 to 10.9% and isoform RhaB2 to 36.0% residual activity; 1 mM reduces the activity of isoform RhaB1 to 10.9% residual activity Lactiplantibacillus plantarum
SDS SDS produces partial inactivation; SDS produces partial inactivation Lactiplantibacillus plantarum
thiorphan less effective inhibitor; less effective inhibitor Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
4-nitrophenyl alpha-L-rhamnopyranoside isoform RhaB2 Lactiplantibacillus plantarum
0.57
-
4-nitrophenyl alpha-L-rhamnopyranoside isoform RhaB1 Lactiplantibacillus plantarum
0.78
-
hesperidin isoform RhaB2 Lactiplantibacillus plantarum
1.06
-
rutin isoform RhaB2 Lactiplantibacillus plantarum
1.77
-
rutin isoform RhaB1 Lactiplantibacillus plantarum
3.39
-
hesperidin isoform RhaB1 Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Lactiplantibacillus plantarum
Co2+
-
Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum C4PG45 isoform RhaB2
-
Lactiplantibacillus plantarum C4PG47 isoform RhaB1
-
Lactiplantibacillus plantarum NCC245 C4PG45 isoform RhaB2
-
Lactiplantibacillus plantarum NCC245 C4PG47 isoform RhaB1
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap chelating column chromatography, gel filtration Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
Lactiplantibacillus plantarum 4-nitrophenol + alpha-L-rhamnopyranose
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
Lactiplantibacillus plantarum NCC245 4-nitrophenol + alpha-L-rhamnopyranose
-
?
4-nitrophenyl alpha-L-rhamnoside + H2O
-
Lactiplantibacillus plantarum alpha-L-rhamnose + 4-nitrophenol
-
?
4-nitrophenyl alpha-L-rhamnoside + H2O
-
Lactiplantibacillus plantarum NCC245 alpha-L-rhamnose + 4-nitrophenol
-
?
hesperidin + H2O 11.2% relative activity compared to rutin Lactiplantibacillus plantarum hesperetin 7-O-glucoside + alpha-L-rhamnose
-
?
hesperidin + H2O isoform RhaB2 exhibits highest activity against hesperidin Lactiplantibacillus plantarum hesperetin 7-O-glucoside + alpha-L-rhamnose
-
?
hesperidin + H2O isoform RhaB2 exhibits highest activity against hesperidin Lactiplantibacillus plantarum NCC245 hesperetin 7-O-glucoside + alpha-L-rhamnose
-
?
hesperidin + H2O 11.2% relative activity compared to rutin Lactiplantibacillus plantarum NCC245 hesperetin 7-O-glucoside + alpha-L-rhamnose
-
?
additional information no activity is detected towards the alpha-1,2 linkage in naringin Lactiplantibacillus plantarum ?
-
?
additional information no activity towards quercitin, gellan gum, vitexin 2-O-rhamnoside, and alpha-chaconine Lactiplantibacillus plantarum ?
-
?
additional information no activity is detected towards the alpha-1,2 linkage in naringin Lactiplantibacillus plantarum NCC245 ?
-
?
additional information no activity towards quercitin, gellan gum, vitexin 2-O-rhamnoside, and alpha-chaconine Lactiplantibacillus plantarum NCC245 ?
-
?
proscillaridin A + H2O 0.4% relative activity compared to hesperidin Lactiplantibacillus plantarum ?
-
?
proscillaridin A + H2O 4.5% relative activity compared to rutin Lactiplantibacillus plantarum ?
-
?
rutin + H2O 10.1% relative activity compared to hesperidin Lactiplantibacillus plantarum isoquercitrin + alpha-L-rhamnose
-
?
rutin + H2O isoform RhaB1 exhibits highest activity against rutin Lactiplantibacillus plantarum isoquercitrin + alpha-L-rhamnose
-
?

Synonyms

Synonyms Comment Organism
RhaB1 isoform Lactiplantibacillus plantarum
RhaB2 isoform Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
isoform RhaB1 Lactiplantibacillus plantarum
60
-
isoform RhaB2 Lactiplantibacillus plantarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 the enzyme shows relative activities over 64% from 30 to 60°C Lactiplantibacillus plantarum
50 70 isoform RhaB2 is most active at temperatures between 50 and 70°C, still showing 70% relative activity at 70°C Lactiplantibacillus plantarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 65 isoform RhaB2 retains 43% and 25% activity after 5 h incubation at 55°C and 65°C, respectively Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
isoform RhaB2 Lactiplantibacillus plantarum
7
-
isoform RhaB1 Lactiplantibacillus plantarum

pH Range

pH Minimum pH Maximum Comment Organism
4.4 5.5 isoform RhaB2 maintains over 60% of its activity in the pH range from 4.4 to 5.5 Lactiplantibacillus plantarum
5 7.5 isoform RhaB1 maintains over 60% of its activity in the pH range from 5.0 to 7.5 Lactiplantibacillus plantarum

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 isoform RhaB1 remains active over a broad range of pH values, showing 90-100% remaining relative activities from pH 4.0 to 7.5, and over 40% at pH 3 and 9, after 5 h at 37°C Lactiplantibacillus plantarum
3 9 the stability of isoform RhaB2 is lower than that of isoform RhaB1, with 80-85% enzymatic activity from pH 5.0 to 7.0, and only 17% and 12% at pH 3.0 and 9.0, respectively Lactiplantibacillus plantarum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
19.88
-
L-rhamnose isoform RhaB1 Lactiplantibacillus plantarum

Expression

Organism Comment Expression
Lactiplantibacillus plantarum the enzyme activity is downregulated by D-glucose down
Lactiplantibacillus plantarum the enzyme activity is upregulated by L-rhamnose up