Cloned (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | 1 mM, 4% residual activity; 1 mM, 70% residual activity | Bacillus sp. (in: Bacteria) | |
D-glucose | 10 mM, 56% residual activity | Bacillus sp. (in: Bacteria) | |
FeCl3 | 1 mM, 76% residual activity | Bacillus sp. (in: Bacteria) | |
HgCl2 | 0.1 mM, 0.23% residual activity | Bacillus sp. (in: Bacteria) | |
L-rhamnose | 10 mM, 14% residual activity; 10 mM, 37% residual activity | Bacillus sp. (in: Bacteria) | |
p-chloromercuribenzoic acid | 0.1 mM, 50% residual activity | Bacillus sp. (in: Bacteria) | |
ZnCl2 | 1 mM, 73% residual activity | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.119 | - |
p-nitrophenyl-alpha-L-rhamnopyranoside | pH 7.0 | Bacillus sp. (in: Bacteria) | |
0.282 | - |
p-nitrophenyl-alpha-L-rhamnopyranoside | pH 7.0 | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
5 * 98300, deduced from gene sequence, 5 * 98000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
98300 | - |
5 * 98300, deduced from gene sequence, 5 * 98000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
100000 | - |
gel filtration, PAGE | Bacillus sp. (in: Bacteria) |
105000 | - |
1 * 106000, deduced from gene sequence, 1 * 105000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
106000 | - |
1 * 106000, deduced from gene sequence, 1 * 105000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
500000 | - |
gel filtration, PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus sp. (in: Bacteria) | isozyme plays a crucial role in complete metabolism of gellan | ? | - |
? | |
additional information | Bacillus sp. (in: Bacteria) GL1 | isozyme plays a crucial role in complete metabolism of gellan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | Q93RE7 | ene rhaB, when grown on gellan medium, only this isoform is expressed | - |
Bacillus sp. (in: Bacteria) | Q93RE8 | gene rhaA | - |
Bacillus sp. (in: Bacteria) GL1 | Q93RE7 | ene rhaB, when grown on gellan medium, only this isoform is expressed | - |
Bacillus sp. (in: Bacteria) GL1 | Q93RE8 | gene rhaA | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | - |
Bacillus sp. (in: Bacteria) |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54 | - |
pH 7.0 | Bacillus sp. (in: Bacteria) |
54.4 | - |
pH 7.0 | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gellan + H2O | - |
Bacillus sp. (in: Bacteria) | L-rhamnose + ? | - |
? | |
gellan + H2O | - |
Bacillus sp. (in: Bacteria) GL1 | L-rhamnose + ? | - |
? | |
additional information | isozyme plays a crucial role in complete metabolism of gellan | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | isozyme plays a crucial role in complete metabolism of gellan | Bacillus sp. (in: Bacteria) GL1 | ? | - |
? | |
naringin + H2O | - |
Bacillus sp. (in: Bacteria) | L-rhamnose + ? | - |
? | |
naringin + H2O | - |
Bacillus sp. (in: Bacteria) GL1 | L-rhamnose + ? | - |
? | |
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) | p-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
p-nitrophenyl-alpha-L-rhamnopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) GL1 | p-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
p-nitrophenyl-beta-D-fucopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) | p-nitrophenol + beta-D-fucopyranose | 2.9% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside | ? | |
p-nitrophenyl-beta-D-fucopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) GL1 | p-nitrophenol + beta-D-fucopyranose | 2.9% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside | ? | |
p-nitrophenyl-beta-D-galactopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) | p-nitrophenol + beta-D-galactopyranose | 1.6% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 106000, deduced from gene sequence, 1 * 105000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
pentamer | 5 * 98300, deduced from gene sequence, 5 * 98000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Bacillus sp. (in: Bacteria) |