Literature summary for 3.2.1.37 extracted from

Zhuo, R.; Yu, H.; Qin, X.; Ni, H.; Jiang, Z.; Ma, F.; Zhang, X.
Heterologous expression and characterization of a xylanase and xylosidase from white rot fungi and their application in synergistic hydrolysis of lignocellulose (2018), Chemosphere, 212, 24-33 .

Search for more literature for 3.2.1.37

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris GS115	Irpex lacteus

Protein Variants

Protein Variants	Comment	Organism
biofuel production	potential application in the field of biomass pretreatment and biofuel production	Irpex lacteus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.15	-	4-nitrophenyl beta-D-xylopyranoside	pH 5.0, 40°C	Irpex lacteus
1.31	-	4-nitrophenyl alpha-L-arabinofuranoside	pH 5.0, 40°C	Irpex lacteus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	SDS-PAGE	Irpex lacteus
365400	-	calculated from sequence	Irpex lacteus

Organism

Organism	UniProt	Comment	Textmining
Irpex lacteus	A0A2U9Q8M3	-	-
Irpex lacteus CD2	A0A2U9Q8M3	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Irpex lacteus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O	low activity	Irpex lacteus	4-nitrophenol + alpha-L-arabinofuranose	-	?
4-nitrophenyl alpha-L-arabinofuranoside + H2O	low activity	Irpex lacteus CD2	4-nitrophenol + alpha-L-arabinofuranose	-	?
4-nitrophenyl beta-D-xylopyranoside + H2O	-	Irpex lacteus	4-nitrophenol + beta-D-xylopyranose	-	?
4-nitrophenyl beta-D-xylopyranoside + H2O	-	Irpex lacteus CD2	4-nitrophenol + beta-D-xylopyranose	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, SDS-PAGE	Irpex lacteus

Synonyms

Synonyms	Comment	Organism
beta-xylosidase	-	Irpex lacteus
XylCD2	-	Irpex lacteus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Irpex lacteus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	50	30°C: optimum, 50°C: about 50% of maximal activity, substrate: 4-nitrophenyl beta-D-xylopyranoside. 30°C: optimum, 50°C: about 25% of maximal activity, substrate: 4-nitrophenyl alpha-L-arabinofuranoside	Irpex lacteus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.81	-	4-nitrophenyl alpha-L-arabinofuranoside	pH 5.0, 40°C	Irpex lacteus
9.4	-	4-nitrophenyl beta-D-xylopyranoside	pH 5.0, 40°C	Irpex lacteus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Irpex lacteus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	pH 5.0: about 60% of maximal activity, pH 8.0: about 30% of maximal activity, substrate: 4-nitrophenyl beta-D-xylopyranoside. pH 5.0: about 60% of maximal activity, pH 8.0: about 40% of maximal activity, substrate: 4-nitrophenyl alpha-L-arabinofuranoside	Irpex lacteus

General Information

General Information	Comment	Organism
physiological function	endo-xylanase and beta-xylosidase are the major enzymes for hemicellulose hydrolysis, which play a significant role in biomass conversion	Irpex lacteus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.14	-	4-nitrophenyl alpha-L-arabinofuranoside	pH 5.0, 40°C	Irpex lacteus
8.18	-	4-nitrophenyl beta-D-xylopyranoside	pH 5.0, 40°C	Irpex lacteus

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Release 2023.1 (January 2023)