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Enzyme Nomenclature
Information on EC 3.2.1.37 - xylan 1,4-beta-xylosidase
for references in articles please use BRENDA:EC3.2.1.37
Word Map on EC 3.2.1.37
- 3.2.1.37
- xylans
- xylose
-
arabinoxylans
- xylobiose
- arabinose
- niger
- cellulase
- xylooligosaccharides
-
xylanolytic
- p-nitrophenyl
- xylanases
- trichoderma
-
hemicellulases
- arabinans
- endoxylanase
- spelt
- beta-d-xylopyranoside
- reesei
- endoglucanase
- pumilus
-
saccharification
- beta-d-galactosidase
- arabinogalactans
- cellobiohydrolase
-
arabinosidase
- beta-xylanase
- p-nitrophenyl-beta-d-xylopyranoside
-
xylan-degrading
-
feruloyl
-
birchwood
- thermomonospora
- endo-1,4-beta-xylanase
- l-arabitol
- fibrisolvens
-
thermoanaerobacterium
-
selenomonas
-
hemicellulolytic
- ruminantium
-
xylopyranosyl
- alpha-glucuronidase
-
bagasse
- cmcases
- saccharolyticum
- alpha-d-mannosidase
- beta-d-fucosidase
- butyrivibrio
- xylotetraose
- alpha-d-galactosidase
-
avicel
- nutrition
- analysis
-
xylanohydrolase
- synthesis
- biotechnology
- degradation
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
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EC NUMBER 
COMMENTARY 
3.2.1.37
-
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RECOMMENDED NAME
GeneOntology No.
xylan 1,4-beta-xylosidase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
Also hydrolyses xylobiose. Some other exoglycosidase activities have been found associated with this enzyme in sheep liver
-
-
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
mechanism
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
kinetics
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
ordered bi-bi mechanism, kinetics
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
Glu160 is the acid-base catalyst, kinetics
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
retention of anomeric configuration, two-step mechanism, SN2-like transition state
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
mechanism
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
catalytic residues D15, D128, and E187 are essential for activity, reaction mechanism
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
hydrolysis of 4-nitrophenyl-beta-D-xylopyranoside with overall retention of substrate anomeric configuration, double-displacement mechanism. Deglycosylation is the rate-limiting step for the enzymatic hydrolysis
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
active site residues are D242 and E441
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
active site residues are D264 and E464
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
hydrolysis of 4-nitrophenyl-beta-D-xylopyranoside with overall retention of substrate anomeric configuration, double-displacement mechanism. Deglycosylation is the rate-limiting step for the enzymatic hydrolysis
-
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
Glu160 is the acid-base catalyst, kinetics
-
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
retention of anomeric configuration, two-step mechanism, SN2-like transition state
-
-
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
ordered bi-bi mechanism, kinetics
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
4-beta-D-xylan xylohydrolase
Also hydrolyses xylobiose. Some other exoglycosidase activities have been found associated with this enzyme in sheep liver.
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CAS REGISTRY NUMBER
COMMENTARY
9025-53-0
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
production of enzyme when grown in liquid-state cultures on media containing oat spelt xylan as the carbon source
-
-
multifunctional enzyme with glucosidase, xylosidase, arabinosidase and cellobiosidase activities
-
-
bifunctional beta-xylosidase/alpha-arabinofuranosidase
UniProt
bifunctional beta-xylosidase/alpha-arabinofuranosidase
UniProt
AHT-1, optimum production of enzyme in medium containing oat-spelt xylan at 30°C, pH 7.0 after 6 days
-
-
Penicillium wortmanni
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
-
-
mutant strain NP54376 exhibiting high xylanolytic activity when grown on various xylan sources, maximal enzyme production by growth on xylan as the sole carbon source
-
-
bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase; soft rot fungus
UniProt
bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase; soft rot fungus
UniProt
Bxl1; bifunctional beta-D-xylosidase/alpha-L-arabinofuranosidase
SwissProt
coexpression of enzyme with Trichoderma reesei xylanayse II in Saccharomyces cerevisiae
SwissProt
i.e. Bacillus stearothermophilus, strain T-6, gene xynB3
SwissProt
bifunctional beta-xylosidase/alpha-L-arabinofuranosidase, EC 3.2.1.37/EC 3.2.1.55
UniProt
OPC-520, growth on xylan or xylooligosaccharides
SwissProt
Rubg3A, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
UniProt
Rubg3B, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
UniProt
RuBGX1, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.21, three catalytic amino acids of RuBGX1 are identified , as D272 is the catalytic nucleophile, and E167 and E481 are the catalytic acids; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
D3VYD8
UniProt
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
physiological function
metabolism
-
beta-xylosidase combined with a xylanase had an additive effect on beechwood xylan hydrolysis, generating xylose as a final product
metabolism
-
beta-xylosidase combined with a xylanase had an additive effect on beechwood xylan hydrolysis, generating xylose as a final product
-
physiological function
-
the beta-xylosidase exhibits synergism with endoxylanase upon hydrolysis of glucuronoxylan as well as with alpha-L-arabinofuranosidase and endoxylanase upon hydrolysis of arabinoxylan. Addition of beta-xylosidases increases efficiency of hydrolysis of plant raw materials with high hemicellulose content such as maize cobs by the enzymic preparation Celloviridine G20x depleted of its own beta-xylosidase
physiological function
-
the beta-xylosidase exhibits synergism with endoxylanase upon hydrolysis of glucuronoxylan as well as with alpha-L-arabinofuranosidase and endoxylanase upon hydrolysis of arabinoxylan. Addition of beta-xylosidases increases efficiency of hydrolysis of plant raw materials with high hemicellulose content such as maize cobs by the enzymic preparation Celloviridine G20x depleted of its own beta-xylosidase
physiological function
when incubated in combination with bifunctional xylanase-ferulic acid esterase Xyn10D-Fae1A, Xyl3A improves the release of xylose monomers from a hemicellulosic xylan substrate, suggesting that these two enzymes function synergistically to depolymerize xylan
physiological function
in T-DNA insertion mutant, mucilage release is both patchy and slow and seeds display delayed germination. Developmental analysis of mutant seeds reveals no change in mucilage secretory cell morphology, but changes in monosaccharide quantities are detected. Chemical and immunological analyses of mucilage extracted from bxl1 mutant seeds and antibody staining of developing seed coats reveal an increase in (1-5)-linked arabinans, suggesting that BXL1 is acting as an a-L-arabinofuranosidase in the seed coat
physiological function
RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3A with an activity ratio of 10:1 (xylosidase/xylanase) increases the liberation to 218% of that in the presence of XynR8 alone; RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3B with an activity ratio of 5:1 (xylosidase/xylanase) increases the liberation to 169% of that in the presence of XynR8 alone. RuBG3B shows no synergism with xylanase XynR8 at the optimal pH 7.5, but at pH 5.5, indicating that RuBG3B may have different pH preferences between aryl xylosidic substrate 4-nitrophenyl-beta-D-xylopyranoside and xylooligisaccharide xylan
physiological function
-
strain grows well on locust bean galactomannan, Solka floc, wheat bran and pectin and at 45°C
physiological function
-
strain grows well on locust bean galactomannan, Solka floc, wheat bran and pectin and at 45°C
physiological function
-
screening of thermophilic and thermotolerant filamentous fungi for the production of beta-xylosidase and arabinanase at different temperatures. beta-Xylosidase and arabinanase activities from mycelial extract (intracellular form) are higher in cultures grown at high temperatures (35-40°C), while the correspondent extracellular activities are favorably secreted from cultures at 30°C. Aspergillus niger and Aspergillus thermomutatus are the filamentous fungi with the most expressive production of beta-xylosidase and arabinanase, respectively
physiological function
-
growth on corncobs (coarse) induces the highest titres of xylosidase and acetyl esterase
physiological function
-
strain grows well on locust bean galactomannan, Solka floc, wheat bran and pectin and at 45°C
-
physiological function
-
strain grows well on locust bean galactomannan, Solka floc, wheat bran and pectin and at 45°C
-
physiological function
-
growth on corncobs (coarse) induces the highest titres of xylosidase and acetyl esterase
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O
2-hydroxymethylphenol + beta-D-glucose
-
low activity
-
-
?
1,4-beta-D-xylobiose + H2O
2 D-xylose
-
thermodynamic parameters for hydrolysis. Rate enhancement kcat/knon, increases with decreasing temperature
-
-
?
2 D-xylose
xylobiose + H2O
-
-
and higher xylooligosaccharides, immobilized enzyme in presence of high concentration of xylose
-
r
2,4-dinitrophenyl 2-deoxy-2-fluoro-beta-D-xyloside + H2O
2,4-dinitrophenol + 2-deoxy-2-fluoro-beta-D-xylose
-
-
-
-
?
2,4-dinitrophenyl-1-thio-beta-D-xylopyranoside + H2O
2,4-dinitrophenol + 1-thio-beta-D-xylopyranose
Penicillium wortmanni
-
-
-
?
2,5-dinitrophenyl-beta-D-xylopyranoside + H2O
2,5-dinitrophenol + beta-D-xylose
-
-
-
-
?
2-nitrophenyl xylopyranoside + H2O
2-nitrophenol + xylopyranose
-
-
-
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O
beta-D-glucose + D-glucose
-
sophorose, low activity
-
?
3,4-dimethylphenyl-beta-D-xylopyranoside + H2O
3,4-dimethylphenol + beta-D-xylopyranose
-
-
-
-
?
3,4-dinitrophenyl beta-D-xylopyranoside + H2O
3,4-dinitrophenol + D-xylopyranose
-
-
-
?
3,5-dichlorophenyl-beta-D-xylopyranoside + H2O
3,5-dicholrophenol + beta-D-xylopyranose
-
-
-
-
?
3,5-dinitrophenyl-beta-D-xylopyranoside + H2O
3,5-dinitrophenol + beta-D-xylose
-
-
-
-
?
3-chlorophenyl-beta-D-xylopyranoside + H2O
3-chlorophenol + beta-D-xylopyranose
-
-
-
-
?
3-nitrophenyl beta-D-xylopyranoside + H2O
3-nitrophenol + D-xylopyranose
-
-
-
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O
beta-D-glucose + D-glucose
-
laminaribiose, low activity
-
?
4-bromophenyl-beta-D-xylopyranoside + H2O
4-bromophenol + beta-D-xylopyranose
-
-
-
-
?
4-bromophenyl-beta-D-xylopyranoside + H2O
4-bromophenol + beta-D-xylose
-
low activity of mutant E178G, no activity of the wild-type enzyme
-
-
?
4-chloro-2-nitrophenyl beta-D-xylopyranoside + H2O
4-chloro-2-nitrophenol + beta-D-xylopyranose
-
-
-
?
4-chloro-2-nitrophenyl-beta-D-xylopyranoside + H2O
4-chloro-2-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-cyanophenyl-beta-D-xylopyranoside + H2O
4-cyanophenol + beta-D-xylopyranose
-
-
-
-
?
4-methylumbelliferyl-alpha-L-arabinofuranoside + H2O
4-methylumbelliferol + alpha-L-arabinofuranose
-
-
-
-
?
4-methylumbelliferyl-beta-D-xylopyranoside + H2O
4-methylumbelliferone + beta-D-xylopyranose
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + D-galactose
-
0.3% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl alpha-D-mannoside + H2O
4-nitrophenol + mannose
-
0.4% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + L-arabinofuranose
-
14.4% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinose
3.2% activity compared to 4-nitrophenyl alpha-D-xylopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
xylan-inducible enzyme shows 10% of activity with 4-nitrophenyl-beta-D-xylopyranoside, and xylose-inducible enzyme 2%
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + methanol
4-nitrophenol + methyl beta-D-xylopyranoside
-
transxylosylation activity, NMR study in the presence of 15% methanol, v/v
very weak peaks of D-xylose-alpha-anomers, most D-xylose-beta-anomers
?
4-nitrophenyl beta-D-xyloside + H2O
4-nitrophenol + D-xylose
-
100% activity
-
-
?
4-nitrophenyl xylopyranoside + H2O
4-nitrophenol + xylopyranose
-
-
-
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
-
cellobiose, 30% activity
-
?
6-bromo-2-naphthyl beta-D-xylopyranoside + H2O
6-bromo-2-naphthol + beta-D-xylopyranose
-
-
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
-
gentiobiose, low activity
-
?
arabinoxylan + H2O
D-xylose + L-arabinose
-
source of substrate: sugar beet
-
-
?
beta(1-4)xylobiose + H2O
beta-D-xylose + beta(1-3)xylobiose + beta(1-4)xylotriose + O-beta-D-xylopyranosyl(1-3)-O-beta-D-xylopyranosyl(1-4)-D-xylopyranose + O-beta-D-xylopyranosyl(1-4)[O-beta-D-xylopyranosyl(1-3)]O-beta-D-xylopyranosyl(1-4)-D-xylopyranose
Penicillium wortmanni
-
-
transxylosylation products
?
beta-1,3-xylan + H2O
beta-D-xylose + ?
-
complete hydrolysis after prolonged incubation
-
?
birchwood xylan + H2O
?
1.8% activity compared to 4-nitrophenyl alpha-D-xylopyranoside
-
-
?
cell wall polysaccharide from alfalfa root + H2O
xylose + arabinose
-
-
-
-
?
laminaribose + H2O
?
-
17.5% activity compared to xylobiose
-
-
?
laminaritriose + H2O
?
-
14.4% activity compared to xylobiose
-
-
?
m-nitrophenyl beta-D-xyloside + H2O
m-nitrophenol + beta-D-xylose
Malbranchea pulchella var. sulfurea
-
low activity
-
?
m-nitrophenyl-beta-D-xylopyranoside + H2O
m-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
Manalpha(1-6)[Manalpha(1-3)Xylbeta(1-2)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc--pyridylaminoside + H2O
beta-D-xylose + Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
complete hydrolysis
-
?
methyl beta-D-xylopyranoside + H2O
methanol + beta-D-xylopyranose
Penicillium wortmanni
-
-
-
?
n-pentyl beta-D-xylopyranoside + H2O
n-pentanol + beta-D-xylopyranose
Penicillium wortmanni
-
-
-
?
o-N-acetylamino-phenyl-beta-D-xylopyranoside + H2O
o-N-acetylamino-phenol + beta-D-xylopyranose
-
-
-
-
?
o-nitrophenyl beta-D-galactopyranoside + H2O
o-nitrophenol + beta-D-galactose
-
slight activity
-
?
p-chlorophenyl beta-D-xyloside + H2O
p-chlorophenol + beta-D-xylose
-
-
-
?
p-methoxyphenyl-beta-D-xylopyranoside + H2O
p-methoxyphenol + beta-D-xylopyranose
-
-
-
-
?
p-nitrophenyl alpha-L-arabinoside + H2O
p-nitrophenol + alpha-L-arabinose
-
low activity
-
?
p-nitrophenyl beta-D-xylopyranoside + beta-D-xylopyranoside
p-nitrophenyl beta-(1-4)-D-xylooligosaccharide + H2O
-
-
2-7 xylopyranose molecules per oligosaccharide
-
r
p-nitrophenyl-alpha-D-arabinoside + H2O
p-nitrophenol + alpha-D-arabinose
-
-
-
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
p-nitrophenol + alpha-L-arabinofuranose
-
5% of activity compared to p-nitrophenyl-beta-D-xylopyranoside
-
-
?
p-nitrophenyl-beta-D-arabinofuranoside + H2O
p-nitrophenol + beta-D-arabinofuranose
8.8% of the acticvity with p-nitrophenyl-beta-D-xylopyranoside
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
p-nitrophenol + beta-D-cellobiose
-
6% of activity compared to p-nitrophenyl-beta-D-xylopyranoside
-
-
?
p-nitrophenyl-beta-D-fucoside + H2O
p-nitrophenol + beta-D-fucose
-
-
-
-
?
p-nitrophenyl-beta-D-xyloside + H2O
p-nitrophenol + alpha-D-xylose
Malbranchea pulchella var. sulfurea
-
beta-xylosidase-1
inversion of anomeric configuration
?
phenyl beta-D-glucopyranoside + H2O
phenol + D-glucopyranose
-
low activity
-
?
phenyl beta-D-xylopyranoside + methanol
methyl beta-D-xylopyranoside + phenol
Penicillium wortmanni
-
in the presence of 1 M methanol, transxylosylation activity
-
?
phenyl beta-D-xylospyranoside + H2O
phenol + beta-D-xylopyranose
-
-
-
?
phenyl-4-O-beta-D-glucopyranosyl-D-glucose + H2O
phenyl-4-O-beta-D-glucose + D-glucose
-
25% activity
-
?
Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl + H2O
4 D-xylose
-
-
-
-
?
Xylbeta(1-2)Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside + H2O
beta-D-xylose + Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
complete hydrolysis
-
?
xylo-oligosaccharides + H2O
xylobiose + ?
-
-
major hydrolysis product
-
?
xylopentaose + H2O
xylose
-
compared to xylobiose, relative rate of xylose release is 75%
-
-
?
xylotetraose + H2O
xylose
-
compared to xylobiose, relative rate of xylose release is 81%
-
-
?
xylotriose + H2O
xylose
-
compared to xylobiose, relative rate of xylose release is 90%
-
-
?
xylotriose + H2O
xylotetraose + xylopentaose
-
transxylosylation activity
-
?
[Manalpha(1-6)Manalpha(1-3)Xylbeta(1-2)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside + H2O
beta-D-xylose + [Manalpha(1-6)Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
50% hydrolysis
-
?
2,4-dinitrophenyl beta-D-xylopyranoside + H2O
2,4-dinitrophenol + beta-D-xylopyranose
Penicillium wortmanni
-
-
-
?
2,4-dinitrophenyl beta-D-xylopyranoside + H2O
2,4-dinitrophenol + beta-D-xylopyranose
-
broad tolerance for the aglycone portion of the substrate
-
?
2,4-dinitrophenyl-beta-D-xylopyranoside + H2O
2,4-dinitrophenol + beta-D-xylopyranose
-
-
-
-
?
2,4-dinitrophenyl-beta-D-xylopyranoside + H2O
2,4-dinitrophenol + beta-D-xylopyranose
-
-
-
-
?
2,5-dinitrophenyl-beta-D-xylopyranoside + H2O
2,5-dinitrophenol + D-xylopyranose
-
-
-
-
?
2,5-dinitrophenyl-beta-D-xylopyranoside + H2O
2,5-dinitrophenol + D-xylopyranose
-
-
-
-
?
2,5-dinitrophenyl-beta-D-xylopyranoside + H2O
2,5-dinitrophenol + D-xylopyranose
-
-
-
-
?
2,5-dinitrophenyl-beta-D-xylopyranoside + H2O
2,5-dinitrophenol + D-xylopyranose
-
-
-
-
?
2,5-dinitrophenyl-beta-D-xylopyranoside + H2O
2,5-dinitrophenol + D-xylopyranose
-
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucose
-
1.7% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucose
-
1.7% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
2-nitrophenyl beta-D-glucoside + H2O
2-nitrophenol + D-glucose
-
6.4% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
2-nitrophenyl beta-D-glucoside + H2O
2-nitrophenol + D-glucose
-
6.4% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
2-nitrophenyl beta-D-xylopyranoside + H2O
2-nitrophenol + D-xylose
-
-
-
?
2-nitrophenyl beta-D-xylopyranoside + H2O
2-nitrophenol + D-xylose
-
-
-
?
2-nitrophenyl beta-D-xylopyranoside + H2O
2-nitrophenol + D-xylose
-
3% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
2-nitrophenyl beta-D-xylopyranoside + H2O
2-nitrophenol + D-xylose
-
3% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
2-nitrophenyl-beta-D-xylopyranoside + H2O
2-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
2-nitrophenyl-beta-D-xylopyranoside + H2O
2-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
2-nitrophenyl-beta-D-xylopyranoside + H2O
2-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
2-nitrophenyl-beta-D-xylopyranoside + H2O
2-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
2-nitrophenyl-beta-D-xylopyranoside + H2O
2-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
3,4-dinitrophenyl-beta-D-xylopyranoside + H2O
3,4-dinitrophenol + beta-D-xylopyranose
-
-
-
-
?
3,4-dinitrophenyl-beta-D-xylopyranoside + H2O
3,4-dinitrophenol + beta-D-xylopyranose
-
-
-
-
-
3,4-dinitrophenyl-beta-D-xylopyranoside + H2O
3,4-dinitrophenol + beta-D-xylopyranose
-
-
-
-
?
3,4-dinitrophenyl-beta-D-xylopyranoside + H2O
3,4-dinitrophenol + beta-D-xylopyranose
-
-
-
-
?
3,4-dinitrophenyl-beta-D-xylopyranoside + H2O
3,4-dinitrophenol + beta-D-xylopyranose
-
-
-
-
?
3,4-dinitrophenyl-beta-D-xylopyranoside + H2O
3,4-dinitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-methylumbelliferyl-beta-D-xylopyranoside + H2O
4-methylumbelliferol + beta-D-xylose
-
-
-
-
?
4-methylumbelliferyl-beta-D-xylopyranoside + H2O
4-methylumbelliferol + beta-D-xylose
-
substrate inhibition
-
-
?
4-methylumbelliferyl-beta-D-xylopyranoside + H2O
4-methylumbelliferone + beta-D-xylopyranose
-
-
-
-
?
4-methylumbelliferyl-beta-D-xylopyranoside + H2O
4-methylumbelliferone + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenol-beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
-
-
-
?
4-nitrophenol-beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
-
-
-
?
4-nitrophenyl alpha-D-glucoside + H2O
4-nitrophenol + D-glucose
-
1.5% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl alpha-D-glucoside + H2O
4-nitrophenol + D-glucose
-
1.5% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + D-xylopyranose
-
-
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + D-xylopyranose
-
-
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + D-xylopyranose
-
-
-
-
-
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
38.5% of the activity with 4-nitrophenyl beta-D-xylopyranoside
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
Aureobasidium melanogenum ATCC 20524
38.5% of the activity with 4-nitrophenyl beta-D-xylopyranoside
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
greater catalytic efficiency for hydrolysis of 4-nitrophenyl-alpha-L-arabinofuranoside than of 4-nitrophenyl-beta-D-xylopyranoside
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
poor substrate
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
thermodynamic parameters for hydrolysis. Rate enhancement kcat/knon, increases with decreasing temperature
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
low activity
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
low activity
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
-
less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinopyranose
-
less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
-
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
-
-
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
-
1.4% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
-
1.4% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
11.1% activity compared to beta-D-xylopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
11.1% activity compared to beta-D-xylopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
xylan-inducible enzyme shows 18% of activity with 4-nitrophenyl-beta-D-xylopyranoside, and xylose-inducible enzyme 8%
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
9% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
9% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
low activity
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
23% of activity compared to p-nitrophenyl-beta-D-xylopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
2.5% of the acticvity with p-nitrophenyl-beta-D-xylopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
-
9.6% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
-
9.6% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
hydrolysis with overall retention of substrate anomeric configuration
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
hydrolysis with overall retention of substrate anomeric configuration
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
Aureobasidium melanogenum ATCC 20524
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
substrate for isoform AbfA
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
100% activity
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
100% activity
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
100% activity
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
100% activity
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
greater catalytic efficiency for hydrolysis of 4-nitrophenyl-alpha-L-arabinofuranoside than of 4-nitrophenyl-beta-D-xylopyranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
46% of the activity with 4-nitrophenyl-beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
S7ZRR2;
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
S7ZRR2;
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
low activity levels, measured in whole fly homogenates, substrate concentration of 60 mM in acetate phosphate buffer
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
highest activity
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
highest activity
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
thermodynamic parameters for hydrolysis. Rate enhancement kcat/knon, increases with decreasing temperature
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
highest activity
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
preferred substrate, specific for, xylan-inducible enzyme and xylose-inducible enzyme
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
specific for
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
specific for
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
best substrate
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
best substrate
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
14% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
14% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylopyranose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylopyranose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylopyranose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
15.7% relative activity compared to the beta-glucosidase activity with 4-nitrophenyl beta-D-glucopyranoside as substrate
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
21.1% relative activity compared to the beta-glucosidase activity with 4-nitrophenyl beta-D-glucopyranoside as substrate
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
D3VYD8;
29% of its beta-glucosidase activity against p-nitrophenyl-beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-xyloside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
?
4-nitrophenyl beta-D-xyloside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinose
-
xylan-inducible enzyme shows 18% of activity with 4-nitrophenyl-beta-D-xylopyranoside, and xylose-inducible enzyme 26%
-
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinose
-
-
-
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
4-nitrophenol + alpha-L-arabinose
-
46% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside
-
-
?
beechwood xylan + H2O
?
1.8% activity compared to 4-nitrophenyl alpha-D-xylopyranoside
-
-
?
benzyl-beta-D-xyloside + H2O
phenol + beta-D-xylose
-
low activity
-
?
benzyl-beta-D-xyloside + H2O
phenol + beta-D-xylose
Penicillium wortmanni
-
low activity for xylosidases-2,-3,-4, 45% activity for xylosidase-1
-
?
beta-1,4-xylan + H2O
beta-D-xylose + ?
-
complete hydrolysis after prolonged incubation
-
?
birchwood xylan + H2O
D-xylose
-
initial product, after prolonged hydrolysis, xylo-oligosaccharides are obeserved, too
-
?
birchwood xylan + H2O
D-xylose
-
initial product, after prolonged hydrolysis, xylo-oligosaccharides are obeserved, too
-
?
butyl-beta-D-xyloside
butanol + beta-D-xylose
Penicillium wortmanni
-
low activity
-
?
cyclohexyl-beta-D-xyloside + H2O
cyclohexanol + beta-D-xylose
-
low activity
-
?
cyclohexyl-beta-D-xyloside + H2O
cyclohexanol + beta-D-xylose
Penicillium wortmanni
-
50% activity compared to that for xylobiose
-
?
cyclohexyl-beta-D-xyloside + H2O
cyclohexanol + beta-D-xylose
-
low activity
-
?
ginsenoside Ra1 + H2O
ginsenoside Rb2 + D-xylose
5.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside
-
-
?
ginsenoside Ra1 + H2O
ginsenoside Rb2 + D-xylose
5.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside
-
-
?
ginsenoside Ra2 + H2O
ginsenoside Rc + D-xylose
4.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside
-
-
?
ginsenoside Ra2 + H2O
ginsenoside Rc + D-xylose
4.2% activity compared to 4-nitrophenyl beta-D-xylopyranoside
-
-
?
hemicellulose xylogalacturonan + H2O
hemicellulose xylogalacturonan + beta-D-xylose
the enzyme is involved in reduction of hemicellulose glycan polymers during fruit ripening
-
-
?
hemicellulose xylogalacturonan + H2O
hemicellulose xylogalacturonan + beta-D-xylose
release of single beta-D-xylose monomer from the xylogalacturonan backbone of hemicellulose
-
-
?
methyl-beta-D-xyloside + H2O
beta-D-xylose + methanol
-
very low activity
-
?
methyl-beta-D-xyloside + H2O
beta-D-xylose + methanol
-
-
-
?
methyl-beta-D-xyloside + H2O
beta-D-xylose + methanol
Penicillium wortmanni
-
very low activity
-
?
methyl-beta-D-xyloside + H2O
beta-D-xylose + methanol
-
very low activity
-
?
o-chlorophenyl beta-D-xyloside + H2O
o-chlorophenol + beta-D-xylose
-
-
-
?
o-chlorophenyl beta-D-xyloside + H2O
o-chlorophenol + beta-D-xylose
-
-
-
?
o-methoxyphenyl beta-D-xyloside
o-methoxyphenol + beta-D-xylose
-
-
-
?
o-methoxyphenyl beta-D-xyloside
o-methoxyphenol + beta-D-xylose
-
-
-
?
o-methylphenyl beta-D-xyloside + H2O
o-methylphenol + beta-D-xylose
-
-
-
?
o-methylphenyl beta-D-xyloside + H2O
o-methylphenol + beta-D-xylose
-
-
-
?
o-nitrophenyl beta-D-xylopyranoside + H2O
o-nitrophenol + beta-D-xylopyranose
-
-
-
?
o-nitrophenyl beta-D-xylopyranoside + H2O
o-nitrophenol + beta-D-xylopyranose
-
-
-
?
o-nitrophenyl beta-D-xylopyranoside + H2O
o-nitrophenol + beta-D-xylopyranose
-
-
-
?
o-nitrophenyl beta-D-xylopyranoside + H2O
o-nitrophenol + beta-D-xylopyranose
-
-
-
?
o-nitrophenyl beta-D-xylopyranoside + H2O
o-nitrophenol + beta-D-xylopyranose
-
-
-
?
o-nitrophenyl beta-D-xylopyranoside + H2O
o-nitrophenol + beta-D-xylopyranose
-
-
-
?
o-nitrophenyl-beta-D-xylopyranoside + H2O
o-nitrophenol + beta-D-xylopyranose
-
9% of activity compared to p-nitrophenyl-beta-D-xylopyranoside
-
-
?
o-nitrophenyl-beta-D-xylopyranoside + H2O
o-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
o-nitrophenyl-beta-D-xylopyranoside + H2O
o-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
o-nitrophenyl-beta-D-xyloside + H2O
o-nitrophenol + beta-D-xylose
-
-
-
?
o-nitrophenyl-beta-D-xyloside + H2O
o-nitrophenol + beta-D-xylose
-
-
-
?
o-nitrophenyl-beta-D-xyloside + H2O
o-nitrophenol + beta-D-xylose
-
-
-
?
o-nitrophenyl-beta-D-xyloside + H2O
o-nitrophenol + beta-D-xylose
-
-
-
?
p-methoxyphenyl beta-D-xyloside + H2O
p-methoxyphenol + beta-D-xylose
-
-
-
?
p-methoxyphenyl beta-D-xyloside + H2O
p-methoxyphenol + beta-D-xylose
-
-
-
?
p-methylphenyl beta-D-xyloside + H2O
p-methylphenol + beta-D-xylose
-
-
-
?
p-methylphenyl beta-D-xyloside + H2O
p-methylphenol + beta-D-xylose
-
-
-
?
p-nitrophenyl alpha-L-arabinofuranoside + H2O
p-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
p-nitrophenyl alpha-L-arabinofuranoside + H2O
p-nitrophenol + alpha-L-arabinofuranose
-
low activity
-
?
p-nitrophenyl alpha-L-arabinofuranoside + H2O
p-nitrophenol + alpha-L-arabinofuranose
-
highest activity
-
?
p-nitrophenyl alpha-L-arabinofuranoside + H2O
p-nitrophenol + alpha-L-arabinofuranose
-
low affinity, 70% activity of that for p-nitrophenyl beta-D-xylopyranose
-
?
p-nitrophenyl alpha-L-arabinopyranoside + H2O
p-nitrophenol + alpha-L-arabinopyranose
-
-
-
?
p-nitrophenyl alpha-L-arabinopyranoside + H2O
p-nitrophenol + alpha-L-arabinopyranose
Penicillium wortmanni
-
-
-
?
p-nitrophenyl alpha-L-arabinopyranoside + H2O
p-nitrophenol + alpha-L-arabinopyranose
-
-
-
?
p-nitrophenyl alpha-L-arabinopyranoside + H2O
p-nitrophenol + alpha-L-arabinopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
activity after immobililization on alumina or silica
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
high activity
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
activity depends on carbon source, on which cells are grown
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
Penicillium wortmanni
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
more rapidly hydrolyzed than o-nitrophenyl beta-D-xylopyranoside
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
high specificity, transxylosylation activity
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
Malbranchea pulchella var. sulfurea
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
Malbranchea pulchella var. sulfurea
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
Malbranchea pulchella var. sulfurea
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
Penicillium wortmanni
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
Penicillium wortmanni
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl beta-D-xyloside + H2O
p-nitrophenol + beta-D-xylose
-
-
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
Phlebia radiata 79 / ATCC 64658
-
-
-
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
-
-
-
-
r
phenyl beta-D-xyloside + H2O
phenol + beta-D-xylose
-
with retention of configuration
-
?
phenyl beta-D-xyloside + H2O
phenol + beta-D-xylose
Malbranchea pulchella var. sulfurea
-
-
-
?
phenyl beta-D-xyloside + H2O
phenol + beta-D-xylose
-
with retention of configuration
-
?
phenyl-beta-D-xylopyranoside + H2O
phenol + beta-D-xylopyranose
-
-
-
-
?
phenyl-beta-D-xylopyranoside + H2O
phenol + beta-D-xylopyranose
-
-
-
-
?
vinasse + H2O
D-xylose + ?
-
by-product of ethanol fermentation
12.1% release of xylose by enzyme alone, combination of enzyme and the multicomponent enzyme product Ultraflo L results in 41.9% release of xylose
-
?
vinasse + H2O
D-xylose + ?
-
hydrolysis by an enzyme cocktail containing a 20%:20%:20%:40% mixture and a 25%:25%:25%:25% mixture, respectively, of the GH43 alpha-L-arabinofuranosidase from Humicola insolens, the GH51 alpha-L-arabinofuranosidase from M. giganteus, a GH10 endo-1,4-beta-xylanase from H. insolens, and a GH3 beta-xylosidase from Trichoderma reesei
-
?
vinasse + H2O
D-xylose + ?
-
by-product of ethanol fermentation
7.7% release of xylose by enzyme alone, combination of enzyme and the multicomponent enzyme product Ultraflo L results in 40.8% relaese of xylose
-
?
wheat arabinoxylan + H2O
D-xylose + ?
-
hydrolysis by an enzyme cocktail containing a 20%:20%:20%:40% mixture and a 25%:25%:25%:25% mixture, respectively, of the GH43 alpha-L-arabinofuranosidase from Humicola insolens, the GH51 alpha-L-arabinofuranosidase from M. giganteus, a GH10 endo-1,4-beta-xylanase from H. insolens, and a GH3 beta-xylosidase from Trichoderma reesei
-
?
xylan + H2O
?
0.4% activity compared to 4-nitrophenyl beta-D-xylopyranoside
-
-
?
xylan + H2O
?
0.4% activity compared to 4-nitrophenyl beta-D-xylopyranoside
-
-
?
xylan + H2O
?
-
from corncob, complete hydrolysis to beta-D-xylose in concert with xylanase
-
-
?
xylan + H2O
beta-D-xylose
from oat spelt or birchwood, low activity
-
-
?
xylan + H2O
beta-D-xylose
from oat spelt or birchwood, low activity
-
-
?
xylan + H2O
beta-D-xylose + ?
-
complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose
-
?
xylan + H2O
beta-D-xylose + ?
-
complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose
-
?
xylan + H2O
beta-D-xylose + ?
-
complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose
-
?
xylan + H2O
beta-D-xylose + ?
-
complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose
-
?
xylan + H2O
beta-D-xylose + ?
-
source of substrate: beechwood. Incubation with enzyme gives 32.8% hydrolysis in 4 h, hydrolysis by enzyme combined with xylanase from Anoxybacillus flavithermus BC gives 63.6% conversion after 4 h
-
-
?
xylan + H2O
beta-D-xylose + ?
-
source of substrate: beechwood. Incubation with enzyme gives 32.8% hydrolysis in 4 h, hydrolysis by enzyme combined with xylanase from Anoxybacillus flavithermus BC gives 63.6% conversion after 4 h
-
-
?
xylan + H2O
beta-D-xylose + ?
-
limited capability to produce xylose due to synergistic action of xylanase and beta-xylosidase, synergistic action also needed for libration of acidic substituents
-
-
-
xylo-oligosaccharides + H2O
beta-D-xylose + ?
-
derived from xylan
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
-
derived from xylan
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
-
decrease of activity with increasing polymerization of substrates
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
-
derived from xylan
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
-
derived from xylan
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
-
derived from xylan
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
-
high transferase activity
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
Penicillium wortmanni
-
operating by a double-inversion mechanism
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
-
derived from xylan
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
-
-
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
-
derived from xylan
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
-
smaller xylooligosaccharides from 2-5mers less rapidly hydrolyzed
-
?
xylobiose + H2O
2 beta-D-xylopyranose
30.8% of the activity with 4-nitrophenyl beta-D-xylopyranoside
-
-
?
xylobiose + H2O
2 beta-D-xylopyranose
Aureobasidium melanogenum ATCC 20524
30.8% of the activity with 4-nitrophenyl beta-D-xylopyranoside
-
-
?
xylobiose + H2O
beta-D-xylose
-
-
hydrolysis via retention of anomeric configuration
-
?
xylobiose + H2O
beta-D-xylose
-
-
hydrolysis via retention of anomeric configuration
-
?
xylobiose + H2O
beta-D-xylose + D-xylose
-
90% degradation within 6 to 9 h
-
?
xylobiose + H2O
beta-D-xylose + D-xylose
-
most rapidly hydrolyzed
-
?
xylobiose + H2O
beta-D-xylose + D-xylose
-
transxylosylation activity
-
?
xylobiose + H2O
beta-D-xylose + D-xylose
-
transxylosylation activity
-
?
xylobiose + H2O
beta-D-xylose + D-xylose
Malbranchea pulchella var. sulfurea
-
without forming any xylooligosaccharides
-
?
xylobiose + H2O
beta-D-xylose + D-xylose
Penicillium wortmanni
-
transxylosylation activity
-
?
xylobiose + H2O
beta-D-xylose + D-xylose
Penicillium wortmanni
-
four isoenzymes divided into three groups of action patterns
-
?
xylobiose + H2O
beta-D-xylose + D-xylose
-
-
-
?
xylobiose + H2O
D-xylose
-
the enzyme is highly active on xylobiose
-
-
?
xylohexaose + H2O
6 D-xylose
-
predominant release of xylose
-
?
xylohexaose + H2O
6 D-xylose
-
predominant release of xylose
-
?
xylohexaose + H2O
6 D-xylose
sole products released are xylose monomers
-
-
?
xylohexaose + H2O
beta-D-xylose
-
80% of the activity with xylobiose
-
-
r
xylohexaose + H2O
xylose
-
compared to xylobiose, relative rate of xylose release is 74%
-
-
?
xylohexaose + H2O
xylose
-
compared to xylobiose, relative rate of xylose release is 74%
-
-
?
xylopentaose + H2O
5 D-xylose
sole products released are xylose monomers
-
-
?
xylopentaose + H2O
beta-D-xylose
-
83% of the activity with xylobiose
-
-
r
xylopentaose + H2O
beta-D-xylose + ?
-
extend of hydrolysis varies with chain length of the substrate
-
?
xylopentaose + H2O
beta-D-xylose + ?
-
lower activity than on xylobiose
-
?
xylotetraose + H2O
beta-D-xylose
-
20-30% degradation after 16 h
-
?
xylotetraose + H2O
beta-D-xylose
-
acts by inverting the anomeric configuration
-
?
xylotetraose + H2O
beta-D-xylose
-
86% of the activity with xylobiose
-
-
r
xylotriose + 2 H2O
3 beta-D-xylopyranose
32.8% of the activity with 4-nitrophenyl beta-D-xylopyranoside
-
-
?
xylotriose + 2 H2O
3 beta-D-xylopyranose
Aureobasidium melanogenum ATCC 20524
32.8% of the activity with 4-nitrophenyl beta-D-xylopyranoside
-
-
?
xylotriose + H2O
beta-D-xylose
-
92% of the activity with xylobiose
-
-
r
xylotriose + H2O
beta-D-xylose + xylobiose
-
70-80% degradation after 16 h
-
?
xylotriose + H2O
beta-D-xylose + xylobiose
-
also transxylosylation activity to xylotetraose
-
?
xylotriose + H2O
beta-D-xylose + xylobiose
Malbranchea pulchella var. sulfurea
-
-
-
?
xylotriose + H2O
beta-D-xylose + xylobiose
-
-
-
?
xylotriose + H2O
xylobiose + D-xylose
-
100% activity compared to xylobiose
-
-
?