Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.37 extracted from

  • Zhang, J.; Cui, T.; Li, X.
    Screening and identification of an Enterobacter ludwigii strain expressing an active beta-xylosidase (2018), Ann. Microbiol., 68, 261-271 .
No PubMed abstract available
Search for more literature for 3.2.1.37

Cloned(Commentary)

Cloned (Comment) Organism
full-length protein is expressed in Escherichia coli as an N-terminal or C-terminal His-tagged fusions protein Enterobacter ludwigii

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ 2 mM, about 60% inhibition of N-terminal His-tagged fusions protein, about 55% inhibition of C-terminal His-tagged fusions protein Enterobacter ludwigii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.55
-
 4-nitrophenyl beta-D-xyloside pH 7.0, 50°C, N-terminal His-tagged fusions protein Enterobacter ludwigii
2.8
-
 4-nitrophenyl beta-D-xyloside pH 7.0, 50°C, C-terminal His-tagged fusions protein Enterobacter ludwigii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mM, about 1.8fold activation of N-terminal His-tagged fusions protein, about 2.2fold activation of C-terminal His-tagged fusions protein Enterobacter ludwigii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61290
-
 calculated from sequence Enterobacter ludwigii

Organism

Organism UniProt Comment Textmining
Enterobacter ludwigii W0BU85
-
-
Enterobacter ludwigii LY-62 W0BU85
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xyloside + H2O
-
 Enterobacter ludwigii 4-nitrophenol + beta-D-xylose
-
 ?
4-nitrophenyl beta-D-xyloside + H2O
-
 Enterobacter ludwigii LY-62 4-nitrophenol + beta-D-xylose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Enterobacter ludwigii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 20°C: about 40% of maximal activity, 45°C: about 60% of maximal activity Enterobacter ludwigii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.94
-
 4-nitrophenyl beta-D-xyloside pH 7.0, 50°C, C-terminal His-tagged fusions protein Enterobacter ludwigii
8.51
-
 4-nitrophenyl beta-D-xyloside pH 7.0, 50°C, N-terminal His-tagged fusions protein Enterobacter ludwigii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Enterobacter ludwigii

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8 at pH values ranging from pH 6.8 to 8.0, the relative activities are preserved at levels greater than 80% Enterobacter ludwigii

pI Value

Organism Comment pI Value Maximum pI Value
Enterobacter ludwigii calculated from sequence
-
 5.41

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.48
-
 4-nitrophenyl beta-D-xyloside pH 7.0, 50°C, C-terminal His-tagged fusions protein Enterobacter ludwigii
5.49
-
 4-nitrophenyl beta-D-xyloside pH 7.0, 50°C, N-terminal His-tagged fusions protein Enterobacter ludwigii